Staphylococcus aureus Cas9 is a multiple-turnover enzyme

SaCas9 Requires 5′-NNGRRT-3′ PAM for Sufficient Cleavage and Possesses Higher Cleavage Activity than SpCas9 or FnCpf1 in Human Cells

(2018) Haihua Xie et al.   Biotechnology Journal

The Biology of CRISPR-Cas: Backward and Forward

(2018) Frank Hille et al.   CELL

Functional Insights Revealed by the Kinetic Mechanism of CRISPR/Cas9

(2018) Austin T. Raper et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Bidirectional Degradation of DNA Cleavage Products Catalyzed by CRISPR/Cas9

(2018) Anthony A. Stephenson et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

DNA Unwinding Is the Primary Determinant of CRISPR-Cas9 Activity

(2018) Shanzhong Gong et al.   Cell Reports

SaCas9 Requires 5′-NNGRRT-3′ PAM for Sufficient Cleavage and Possesses Higher Cleavage Activity than SpCas9 or FnCpf1 in Human Cells

(2018) Haihua Xie et al.   Biotechnology Journal

Genome editing via delivery of Cas9 ribonucleoprotein

(2017) Mark A. DeWitt et al.   METHODS

Diversity and evolution of class 2 CRISPR–Cas systems

(2017) Sergey Shmakov et al.   NATURE REVIEWS MICROBIOLOGY

The initiation, propagation and dynamics of CRISPR-SpyCas9 R-loop complex

(2017) Yan Zeng et al.   NUCLEIC ACIDS RESEARCH

Kinetics of dCas9 target search inEscherichia coli

(2017) Daniel Lawson Jones et al.   SCIENCE

Lessons from Enzyme Kinetics Reveal Specificity Principles for RNA-Guided Nucleases in RNA Interference and CRISPR-Based Genome Editing

(2017) Namita Bisaria et al.   Cell Systems

Earth-abundant catalysts for electrochemical and photoelectrochemical water splitting

(2017) Isolda Roger et al.   Nature Reviews Chemistry

CRISPR-Cas9 cleavage efficiency correlates strongly with target-sgRNA folding stability: from physical mechanism to off-target assessment

(2017) Xiaojun Xu et al.   Scientific Reports

CRISPR/Cas9 in Genome Editing and Beyond

(2016) Haifeng Wang et al.   Annual Review of Biochemistry

Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA

(2016) Christopher D Richardson et al.   NATURE BIOTECHNOLOGY

Kinetics of the CRISPR-Cas9 effector complex assembly and the role of 3′-terminal segment of guide RNA

(2016) Vladimir Mekler et al.   NUCLEIC ACIDS RESEARCH

Internal guide RNA interactions interfere with Cas9-mediated cleavage

(2016) Summer B. Thyme et al.   Nature Communications

Crystal Structure of Staphylococcus aureus Cas9

(2015) Hiroshi Nishimasu et al.   CELL

Single-Stranded DNA Cleavage by Divergent CRISPR-Cas9 Enzymes

(2015) Enbo Ma et al.   MOLECULAR CELL

In vivo genome editing using Staphylococcus aureus Cas9

(2015) F. Ann Ran et al.   NATURE

Adapting capillary gel electrophoresis as a sensitive, high-throughput method to accelerate characterization of nucleic acid metabolic enzymes

(2015) Lucia Greenough et al.   NUCLEIC ACIDS RESEARCH

Rational design of a split-Cas9 enzyme complex

(2015) Addison V. Wright et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Cas9-guide RNA complex preorganized for target DNA recognition

(2015) F. Jiang et al.   SCIENCE

De novo assembly of bacterial transcriptomes from RNA-seq data

(2015) Brian Tjaden   GENOME BIOLOGY

CRISPR/Cas9-mediated genome engineering: An adeno-associated viral (AAV) vector toolbox

(2014) Elena Senís et al.   Biotechnology Journal

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

(2014) Hiroshi Nishimasu et al.   CELL

Guide RNA Functional Modules Direct Cas9 Activity and Orthogonality

(2014) Alexandra E. Briner et al.   MOLECULAR CELL

Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease

(2014) Carolin Anders et al.   NATURE

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9

(2014) Samuel H. Sternberg et al.   NATURE

Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation

(2014) John G Doench et al.   NATURE BIOTECHNOLOGY

Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes

(2014) Mark D. Szczelkun et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation

(2014) M. Jinek et al.   SCIENCE

Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

(2014) Steven Lin et al.   eLife

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

A Guide to Simple and Informative Binding Assays

(2010) Thomas D. Pollard   MOLECULAR BIOLOGY OF THE CELL

Short motif sequences determine the targets of the prokaryotic CRISPR defence system

(2009)   MICROBIOLOGY-SGM