标题
Current advances in overcoming obstacles of CRISPR/Cas9 off-target genome editing
作者
关键词
CRISPR/Cas9, Genome-editing, Off-target effects, Base editors, Prime editing, Anti-CRISPR proteins
出版物
MOLECULAR GENETICS AND METABOLISM
Volume -, Issue -, Pages -
出版商
Elsevier BV
发表日期
2021-08-08
DOI
10.1016/j.ymgme.2021.08.002
参考文献
相关参考文献
注意:仅列出部分参考文献,下载原文获取全部文献信息。- Approach for in vivo delivery of CRISPR/Cas system: a recent update and future prospect
- (2021) Yu-Fan Chuang et al. CELLULAR AND MOLECULAR LIFE SCIENCES
- Prime editing – an update on the field
- (2021) Janine Scholefield et al. GENE THERAPY
- Novel Engineered Programmable Systems for ADAR-Mediated RNA Editing
- (2020) Guillermo Aquino-Jarquin Molecular Therapy-Nucleic Acids
- Variability in Genome Editing Outcomes: Challenges for Research Reproducibility and Clinical Safety
- (2020) Lydia Teboul et al. MOLECULAR THERAPY
- Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies
- (2020) Nicole D. Marino et al. NATURE METHODS
- CRISPR off-target detection with DISCOVER-seq
- (2020) Beeke Wienert et al. Nature Protocols
- Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants
- (2020) Russell T. Walton et al. SCIENCE
- Progress and challenges towards CRISPR/Cas clinical translation
- (2020) Daniel Rosenblum et al. ADVANCED DRUG DELIVERY REVIEWS
- Current Status and Challenges of DNA Base Editing Tools
- (2020) You Kyeong Jeong et al. MOLECULAR THERAPY
- CHANGE-seq reveals genetic and epigenetic effects on CRISPR–Cas9 genome-wide activity
- (2020) Cicera R. Lazzarotto et al. NATURE BIOTECHNOLOGY
- CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells
- (2020) Ibrahim C. Kurt et al. NATURE BIOTECHNOLOGY
- The delivery challenge: fulfilling the promise of therapeutic genome editing
- (2020) Joost van Haasteren et al. NATURE BIOTECHNOLOGY
- Engineered CRISPR/Cas9 enzymes improve discrimination by slowing DNA cleavage to allow release of off-target DNA
- (2020) Mu-Sen Liu et al. Nature Communications
- Latest Developed Strategies to Minimize the Off-Target Effects in CRISPR-Cas-Mediated Genome Editing
- (2020) Muhammad Naeem et al. Cells
- Anticipating and Identifying Collateral Damage in Genome Editing
- (2020) Gaëtan Burgio et al. TRENDS IN GENETICS
- CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs
- (2020) Matthew A. Coelho et al. Nature Communications
- Various Aspects of a Gene Editing System—CRISPR–Cas9
- (2020) Edyta Janik et al. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- CRISPR-Cas “Non-Target” Sites Inhibit On-Target Cutting Rates
- (2020) Eirik A. Moreb et al. CRISPR Journal
- Methodologies for Improving HDR Efficiency
- (2019) Mingjie Liu et al. Frontiers in Genetics
- CRISPR-Cas9 genome editing induces megabase-scale chromosomal truncations
- (2019) Grégoire Cullot et al. Nature Communications
- A New Class of Medicines through DNA Editing
- (2019) Matthew H. Porteus NEW ENGLAND JOURNAL OF MEDICINE
- Diverse Mechanisms of CRISPR-Cas9 Inhibition by Type IIC Anti-CRISPR Proteins
- (2019) Yalan Zhu et al. MOLECULAR CELL
- Adenine base editing in an adult mouse model of tyrosinaemia
- (2019) Chun-Qing Song et al. Nature Biomedical Engineering
- Increasing the specificity of CRISPR systems with engineered RNA secondary structures
- (2019) D. Dewran Kocak et al. NATURE BIOTECHNOLOGY
- Optimal sequencing depth design for whole genome re-sequencing in pigs
- (2019) Yifan Jiang et al. BMC BIOINFORMATICS
- Engineering guide RNA to reduce the off-target effects of CRISPR
- (2019) Jing Wu et al. Journal of Genetics and Genomics
- Got mutation? ‘Base editors’ fix genomes one nucleotide at a time
- (2019) Sandeep Ravindran NATURE
- Search-and-replace genome editing without double-strand breaks or donor DNA
- (2019) Andrew V. Anzalone et al. NATURE
- A prime alternative
- (2019) Linda Koch NATURE REVIEWS GENETICS
- Improved Nucleic Acid Therapy with Advanced Nanoscale Biotechnology
- (2019) Yuhua Weng et al. Molecular Therapy-Nucleic Acids
- SaCas9 Requires 5′-NNGRRT-3′ PAM for Sufficient Cleavage and Possesses Higher Cleavage Activity than SpCas9 or FnCpf1 in Human Cells
- (2018) Haihua Xie et al. Biotechnology Journal
- UDiTaS™, a genome editing detection method for indels and genome rearrangements
- (2018) Georgia Giannoukos et al. BMC GENOMICS
- CRISPR–Cas13 Precision Transcriptome Engineering in Cancer
- (2018) Javier T. Granados-Riveron et al. CANCER RESEARCH
- Adenine base editing in mouse embryos and an adult mouse model of Duchenne muscular dystrophy
- (2018) Seuk-Min Ryu et al. NATURE BIOTECHNOLOGY
- Repair of double-strand breaks induced by CRISPR–Cas9 leads to large deletions and complex rearrangements
- (2018) Michael Kosicki et al. NATURE BIOTECHNOLOGY
- Partial DNA-guided Cas9 enables genome editing with reduced off-target activity
- (2018) Hao Yin et al. Nature Chemical Biology
- CRISPR–Cas9 genome editing induces a p53-mediated DNA damage response
- (2018) Emma Haapaniemi et al. NATURE MEDICINE
- Initiation of homologous recombination at DNA nicks
- (2018) Nancy Maizels et al. NUCLEIC ACIDS RESEARCH
- Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
- (2018) Christopher R. Cromwell et al. Nature Communications
- SaCas9 Requires 5′-NNGRRT-3′ PAM for Sufficient Cleavage and Possesses Higher Cleavage Activity than SpCas9 or FnCpf1 in Human Cells
- (2018) Haihua Xie et al. Biotechnology Journal
- In Vivo Genome Editing as a Therapeutic Approach
- (2018) Beatrice Ho et al. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- In vivo CRISPR editing with no detectable genome-wide off-target mutations
- (2018) Pinar Akcakaya et al. NATURE
- Evolved Cas9 variants with broad PAM compatibility and high DNA specificity
- (2018) Johnny H. Hu et al. NATURE
- Directed evolution of CRISPR-Cas9 to increase its specificity
- (2018) Jungjoon K. Lee et al. Nature Communications
- Base editing: precision chemistry on the genome and transcriptome of living cells
- (2018) Holly A. Rees et al. NATURE REVIEWS GENETICS
- Whole genome sequencing reveals rare off-target mutations and considerable inherent genetic or/and somaclonal variations in CRISPR/Cas9-edited cotton plants
- (2018) Jianying Li et al. PLANT BIOTECHNOLOGY JOURNAL
- Staphylococcus aureus Cas9 is a multiple-turnover enzyme
- (2018) Paul Yourik et al. RNA
- Enhanced proofreading governs CRISPR–Cas9 targeting accuracy
- (2017) Janice S. Chen et al. NATURE
- Structural basis of CRISPR–SpyCas9 inhibition by an anti-CRISPR protein
- (2017) De Dong et al. NATURE
- Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage
- (2017) Nicole M. Gaudelli et al. NATURE
- CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR–Cas9 nuclease off-targets
- (2017) Shengdar Q Tsai et al. NATURE METHODS
- Improving CRISPR–Cas specificity with chemical modifications in single-guide RNAs
- (2017) Daniel E Ryan et al. NUCLEIC ACIDS RESEARCH
- Lessons from Enzyme Kinetics Reveal Specificity Principles for RNA-Guided Nucleases in RNA Interference and CRISPR-Based Genome Editing
- (2017) Namita Bisaria et al. Cell Systems
- Disabling Cas9 by an anti-CRISPR DNA mimic
- (2017) Jiyung Shin et al. Science Advances
- Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
- (2016) Alexis C. Komor et al. NATURE
- High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects
- (2016) Benjamin P. Kleinstiver et al. NATURE
- A genome-editing strategy to treat β-hemoglobinopathies that recapitulates a mutation associated with a benign genetic condition
- (2016) Elizabeth A Traxler et al. NATURE MEDICINE
- Detecting DNA double-stranded breaks in mammalian genomes by linear amplification–mediated high-throughput genome-wide translocation sequencing
- (2016) Jiazhi Hu et al. Nature Protocols
- Repurposing the CRISPR-Cas9 system for targeted DNA methylation
- (2016) Aleksandar Vojta et al. NUCLEIC ACIDS RESEARCH
- Selection-free genome editing of the sickle mutation in human adult hematopoietic stem/progenitor cells
- (2016) M. A. DeWitt et al. Science Translational Medicine
- Reprogrammable CRISPR/Cas9-based system for inducing site-specific DNA methylation
- (2016) James I. McDonald et al. Biology Open
- CRISPR-Cas9D10A nickase-based genotypic and phenotypic screening to enhance genome editing
- (2016) Ting-Wei Will Chiang et al. Scientific Reports
- Different Effects of sgRNA Length on CRISPR-mediated Gene Knockout Efficiency
- (2016) Jian-Ping Zhang et al. Scientific Reports
- Genome Editing Technologies: Defining a Path to Clinic
- (2015) Jacqueline Corrigan-Curay et al. MOLECULAR THERAPY
- Chromothripsis from DNA damage in micronuclei
- (2015) Cheng-Zhong Zhang et al. NATURE
- A split-Cas9 architecture for inducible genome editing and transcription modulation
- (2015) Bernd Zetsche et al. NATURE BIOTECHNOLOGY
- Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers
- (2015) Isaac B Hilton et al. NATURE BIOTECHNOLOGY
- Digenome-seq: genome-wide profiling of CRISPR-Cas9 off-target effects in human cells
- (2015) Daesik Kim et al. NATURE METHODS
- Rationally engineered Cas9 nucleases with improved specificity
- (2015) I. M. Slaymaker et al. SCIENCE
- Deletions, Inversions, Duplications: Engineering of Structural Variants using CRISPR/Cas in Mice
- (2015) Katerina Kraft et al. Cell Reports
- siRNA Versus miRNA as Therapeutics for Gene Silencing
- (2015) Jenny K W Lam et al. Molecular Therapy-Nucleic Acids
- Development and Applications of CRISPR-Cas9 for Genome Engineering
- (2014) Patrick D. Hsu et al. CELL
- Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease
- (2014) Carolin Anders et al. NATURE
- Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing
- (2014) Shengdar Q Tsai et al. NATURE BIOTECHNOLOGY
- Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification
- (2014) John P Guilinger et al. NATURE BIOTECHNOLOGY
- GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
- (2014) Shengdar Q Tsai et al. NATURE BIOTECHNOLOGY
- Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases
- (2014) Richard L Frock et al. NATURE BIOTECHNOLOGY
- CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences
- (2014) Y. Lin et al. NUCLEIC ACIDS RESEARCH
- The new frontier of genome engineering with CRISPR-Cas9
- (2014) J. A. Doudna et al. SCIENCE
- Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity
- (2013) F. Ann Ran et al. CELL
- CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
- (2013) Luke A. Gilbert et al. CELL
- Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases
- (2013) S. W. Cho et al. GENOME RESEARCH
- CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering
- (2013) Prashant Mali et al. NATURE BIOTECHNOLOGY
- DNA targeting specificity of RNA-guided Cas9 nucleases
- (2013) Patrick D Hsu et al. NATURE BIOTECHNOLOGY
- High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity
- (2013) Vikram Pattanayak et al. NATURE BIOTECHNOLOGY
- High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
- (2013) Yanfang Fu et al. NATURE BIOTECHNOLOGY
- CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity
- (2013) Thomas J. Cradick et al. NUCLEIC ACIDS RESEARCH
- Mammalian Base Excision Repair: the Forgotten Archangel
- (2013) Grigory L. Dianov et al. NUCLEIC ACIDS RESEARCH
- RNA-Guided Human Genome Engineering via Cas9
- (2013) P. Mali et al. SCIENCE
- Bacteriophage genes that inactivate the CRISPR/Cas bacterial immune system
- (2012) Joe Bondy-Denomy et al. NATURE
- Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria
- (2012) G. Gasiunas et al. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
- A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity
- (2012) M. Jinek et al. SCIENCE
- Short motif sequences determine the targets of the prokaryotic CRISPR defence system
- (2009) MICROBIOLOGY-SGM
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