Microphysiological models of the central nervous system with fluid flow

Utilizing Dynamic Contrast-Enhanced Magnetic Resonance Imaging (DCE-MRI) to Analyze Interstitial Fluid Flow and Transport in Glioblastoma and the Surrounding Parenchyma in Human Patients

(2021) Krishnashis Chatterjee et al.   Pharmaceutics

Studying Human Neurodevelopment and Diseases Using 3D Brain Organoids

(2020) Ai Tian et al.   JOURNAL OF NEUROSCIENCE

Cerebrospinal fluid influx drives acute ischemic tissue swelling

(2020) Humberto Mestre et al.   SCIENCE

Astrocytes and Microglia: In Sickness and in Health

(2020) Ilia D. Vainchtein et al.   TRENDS IN NEUROSCIENCES

Modeling Cell-Cell Interactions in Parkinson’s Disease Using Human Stem Cell-Based Models

(2020) Katrin Simmnacher et al.   Frontiers in Cellular Neuroscience

Identification of region-specific astrocyte subtypes at single cell resolution

(2020) Mykhailo Y. Batiuk et al.   Nature Communications

Decellularized Tissues as Platforms for In Vitro Modeling of Healthy and Diseased Tissues

(2020) Michaela W. McCrary et al.   Acta Biomaterialia

Bioprinting Neural Systems to Model Central Nervous System Diseases

(2020) Boning Qiu et al.   ADVANCED FUNCTIONAL MATERIALS

A primary neural cell culture model to study neuron, astrocyte, and microglia interactions in neuroinflammation

(2020) Noah Goshi et al.   Journal of Neuroinflammation

Modeling Controlled Cortical Impact Injury in 3D Brain‐Like Tissue Cultures

(2020) Volha Liaudanskaya et al.   Advanced Healthcare Materials

Human Cerebrospinal Fluid Induces Neuronal Excitability Changes in Resected Human Neocortical and Hippocampal Brain Slices

(2020) Jenny Wickham et al.   Frontiers in Neuroscience

A 3D human brain–like tissue model of herpes-induced Alzheimer’s disease

(2020) Dana M. Cairns et al.   Science Advances

Bioengineering tissue morphogenesis and function in human neural organoids

(2020) Nikolai J. Fedorchak et al.   SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY

Towards brain-tissue-like biomaterials

(2020) Eneko Axpe et al.   Nature Communications

Human ex vivo spinal cord slice culture as a useful model of neural development, lesion, and allogeneic neural cell therapy

(2020) Chenhong Lin et al.   Stem Cell Research & Therapy

Human iPSC-Derived Hippocampal Spheroids: An Innovative Tool for Stratifying Alzheimer Disease Patient-Specific Cellular Phenotypes and Developing Therapies

(2020) Yuriy Pomeshchik et al.   Stem Cell Reports

TGFβ3 is neuroprotective and alleviates the neurotoxic response induced by aligned poly-l-lactic acid fibers on naïve and activated primary astrocytes

(2020) Manoj K. Gottipati et al.   Acta Biomaterialia

Human Pluripotent Stem Cell-Derived Neural Cells and Brain Organoids Reveal SARS-CoV-2 Neurotropism Predominates in Choroid Plexus Epithelium

(2020) Fadi Jacob et al.   Cell Stem Cell

Negative feedback control of neuronal activity by microglia

(2020) Ana Badimon et al.   NATURE

Astrocyte Reactivity: Subtypes, States, and Functions in CNS Innate Immunity

(2020) Michael V. Sofroniew   TRENDS IN IMMUNOLOGY

3D-bioprinted all-inclusive bioanalytical platforms for cell studies

(2020) Roya Mazrouei et al.   Scientific Reports

Chemically Modified Biopolymers for the Formation of Biomedical Hydrogels

(2020) Victoria G. Muir et al.   CHEMICAL REVIEWS

Three-Dimensional Bioprinted Hyaluronic Acid Hydrogel Test Beds for Assessing Neural Cell Responses to Competitive Growth Stimuli

(2020) Tran B. Ngo et al.   ACS Biomaterials-Science & Engineering

Ciliary Beating Compartmentalizes Cerebrospinal Fluid Flow in the Brain and Regulates Ventricular Development

(2019) Emilie W. Olstad et al.   CURRENT BIOLOGY

Oligodendrocyte Progenitor Cells Become Regionally Diverse and Heterogeneous with Age

(2019) Sonia Olivia Spitzer et al.   NEURON

Pioneer Factor NeuroD1 Rearranges Transcriptional and Epigenetic Profiles to Execute Microglia-Neuron Conversion

(2019) Taito Matsuda et al.   NEURON

Modeling Patient-Derived Glioblastoma with Cerebral Organoids

(2019) Amanda Linkous et al.   Cell Reports

Increasing cellular lifespan with a flow system in organotypic culture of the Laterodorsal Tegmentum (LDT)

(2019) César R. Romero-Leguizamón et al.   Scientific Reports

Flow-enhanced vascularization and maturation of kidney organoids in vitro

(2019) Kimberly A. Homan et al.   NATURE METHODS

Establishing Cerebral Organoids as Models of Human-Specific Brain Evolution

(2019) Alex A. Pollen et al.   CELL

A bioprinted human-glioblastoma-on-a-chip for the identification of patient-specific responses to chemoradiotherapy

(2019) Hee-Gyeong Yi et al.   Nature Biomedical Engineering

Bioengineered scaffolds for 3D culture demonstrate extracellular matrix-mediated mechanisms of chemotherapy resistance in glioblastoma

(2019) Weikun Xiao et al.   MATRIX BIOLOGY

Specification of positional identity in forebrain organoids

(2019) Gustav Y. Cederquist et al.   NATURE BIOTECHNOLOGY

Decreased Cerebrospinal Fluid Flow Is Associated With Cognitive Deficit in Elderly Patients

(2019) Jadwiga Attier-Zmudka et al.   Frontiers in Aging Neuroscience

Engineering Stem Cell Self-organization to Build Better Organoids

(2019) Jonathan A. Brassard et al.   Cell Stem Cell

Mimicking the Brain Extracellular Matrix in Vitro : A Review of Current Methodologies and Challenges

(2019) Rossana Rauti et al.   ISRAEL JOURNAL OF CHEMISTRY

Functionalization of Brain Region-specific Spheroids with Isogenic Microglia-like Cells

(2019) Liqing Song et al.   Scientific Reports

In vivo direct reprogramming of glial linage to mature neurons after cerebral ischemia

(2019) Toru Yamashita et al.   Scientific Reports

The Need for Physiological Micro-Nanofluidic Systems of the Brain

(2019) Jean-Philippe Frimat et al.   Frontiers in Bioengineering and Biotechnology

Brain-on-a-chip: A history of development and future perspective

(2019) Seokyoung Bang et al.   Biomicrofluidics

Assessing drug response in engineered brain microenvironments

(2019) Kinsley M. Tate et al.   BRAIN RESEARCH BULLETIN

Influence of Age on Global and Regional Brain Stiffness in Young and Middle‐Aged Adults

(2019) Tomohiro Takamura et al.   JOURNAL OF MAGNETIC RESONANCE IMAGING

CrossTalk proposal: The glymphatic system supports convective exchange of cerebrospinal fluid and brain interstitial fluid that is mediated by perivascular aquaporin‐4

(2019) Jeffrey Iliff et al.   JOURNAL OF PHYSIOLOGY-LONDON

Microglia affect α-synuclein cell-to-cell transfer in a mouse model of Parkinson’s disease

(2019) Sonia George et al.   Molecular Neurodegeneration

Modeling Alzheimer’s disease with iPSC-derived brain cells

(2019) Jay Penney et al.   MOLECULAR PSYCHIATRY

Electrical and synaptic integration of glioma into neural circuits

(2019) Humsa S. Venkatesh et al.   NATURE

Biofabrication of neural microphysiological systems using magnetic spheroid bioprinting

(2019) Devon Bowser et al.   Biofabrication

Biomanufacturing of organ-specific tissues with high cellular density and embedded vascular channels

(2019) Mark A. Skylar-Scott et al.   Science Advances

Coupled electrophysiological, hemodynamic, and cerebrospinal fluid oscillations in human sleep

(2019) Nina E. Fultz et al.   SCIENCE

Functional maturation of human neural stem cells in a 3D bioengineered brain model enriched with fetal brain-derived matrix

(2019) Disha Sood et al.   Scientific Reports

A simple DMSO-based method for cryopreservation of primary hippocampal and cortical neurons

(2019) Yuta Ishizuka et al.   JOURNAL OF NEUROSCIENCE METHODS

Preparation of dissociated mouse primary neuronal cultures from long-term cryopreserved brain tissue

(2019) M. Cano-Jaimez et al.   JOURNAL OF NEUROSCIENCE METHODS

Engineering geometrical 3-dimensional untethered in vitro neural tissue mimic

(2019) Gelson J. Pagan-Diaz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

An Improved in vitro Model of Cortical Tissue

(2019) Aaron Gilmour et al.   Frontiers in Neuroscience

Three-Dimensional-Bioprinted Dopamine-Based Matrix for Promoting Neural Regeneration

(2018) Xuan Zhou et al.   ACS Applied Materials & Interfaces

An advanced glioma cell invasion assay based on organotypic brain slice cultures

(2018) Tanja Eisemann et al.   BMC CANCER

Docetaxel facilitates lymphatic-tumor crosstalk to promote lymphangiogenesis and cancer progression

(2018) Alexandra R. Harris et al.   BMC CANCER

The choroid plexus epithelium as a novel player in the stomach-brain axis during Helicobacter infection

(2018) N. Gorlé et al.   BRAIN BEHAVIOR AND IMMUNITY

Biomimetic hydrogels direct spinal progenitor cell differentiation and promote functional recovery after spinal cord injury

(2018) Sydney A Geissler et al.   Journal of Neural Engineering

Human brain organoid-on-a-chip to model prenatal nicotine exposure

(2018) Yaqing Wang et al.   LAB ON A CHIP

User-defined local stimulation of live tissue through a movable microfluidic port

(2018) Megan A. Catterton et al.   LAB ON A CHIP

Interstitial Flow Promotes Macrophage Polarization toward an M2 Phenotype

(2018) Ran Li et al.   MOLECULAR BIOLOGY OF THE CELL

A 3D human triculture system modeling neurodegeneration and neuroinflammation in Alzheimer’s disease

(2018) Joseph Park et al.   NATURE NEUROSCIENCE

Fluid Dynamics Inside the Brain Barrier: Current Concept of Interstitial Flow, Glymphatic Flow, and Cerebrospinal Fluid Circulation in the Brain

(2018) Tsutomu Nakada et al.   NEUROSCIENTIST

Glial Cells Shape Pathology and Repair After Spinal Cord Injury

(2018) Andrew D. Gaudet et al.   Neurotherapeutics

The Biology of Regeneration Failure and Success After Spinal Cord Injury

(2018) Amanda Phuong Tran et al.   PHYSIOLOGICAL REVIEWS

Organ-Derived Decellularized Extracellular Matrix: A Game Changer for Bioink Manufacturing?

(2018) Deepak Choudhury et al.   TRENDS IN BIOTECHNOLOGY

Bioprinting for Neural Tissue Engineering

(2018) Stephanie Knowlton et al.   TRENDS IN NEUROSCIENCES

Temporal and Spatial Effects of Blast Overpressure on Blood-Brain Barrier Permeability in Traumatic Brain Injury

(2018) Matthew Kuriakose et al.   Scientific Reports

Towards organogenesis and morphogenesis in vitro: harnessing engineered microenvironment and autonomous behaviors of pluripotent stem cells

(2018) Ningwei Li et al.   Integrative Biology

Functional aspects of meningeal lymphatics in ageing and Alzheimer’s disease

(2018) Sandro Da Mesquita et al.   NATURE

Induction of myelinating oligodendrocytes in human cortical spheroids

(2018) Mayur Madhavan et al.   NATURE METHODS

Bidirectional Microglia–Neuron Communication in Health and Disease

(2018) Zsuzsanna Szepesi et al.   Frontiers in Cellular Neuroscience

Microglia/Astrocytes–Glioblastoma Crosstalk: Crucial Molecular Mechanisms and Microenvironmental Factors

(2018) Diana Matias et al.   Frontiers in Cellular Neuroscience

Astrocytes and microglia: Models and tools

(2018) Kevin A. Guttenplan et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Human microglia regional heterogeneity and phenotypes determined by multiplexed single-cell mass cytometry

(2018) Chotima Böttcher et al.   NATURE NEUROSCIENCE

Glia as architects of central nervous system formation and function

(2018) Nicola J. Allen et al.   SCIENCE

Flow of cerebrospinal fluid is driven by arterial pulsations and is reduced in hypertension

(2018) Humberto Mestre et al.   Nature Communications

Microglia innately develop within cerebral organoids

(2018) Paul R. Ormel et al.   Nature Communications

Neural Progenitor Cell Terminology

(2018) Verónica Martínez-Cerdeño et al.   Frontiers in Neuroanatomy

Convective forces increase CXCR4-dependent glioblastoma cell invasion in GL261 murine model

(2018) R. Chase Cornelison et al.   Scientific Reports

3D Printing of Neural Tissues Derived from Human Induced Pluripotent Stem Cells Using a Fibrin-Based Bioink

(2018) Emily Abelseth et al.   ACS Biomaterials-Science & Engineering

Uniform neural tissue models produced on synthetic hydrogels using standard culture techniques

(2017) Christopher Barry et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

Blood–brain barrier-on-a-chip: Microphysiological systems that capture the complexity of the blood–central nervous system interface

(2017) Duc TT Phan et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

Organotypic cultures of cerebellar slices as a model to investigate demyelinating disorders

(2017) Frédéric Doussau et al.   Expert Opinion on Drug Discovery

Understanding the functions and relationships of the glymphatic system and meningeal lymphatics

(2017) Antoine Louveau et al.   JOURNAL OF CLINICAL INVESTIGATION

Temporal and Spatial Evolution of Raised Intraspinal Pressure after Traumatic Spinal Cord Injury

(2017) Zin Z. Khaing et al.   JOURNAL OF NEUROTRAUMA

A three dimensional in vitro glial scar model to investigate the local strain effects from micromotion around neural implants

(2017) Kevin C. Spencer et al.   LAB ON A CHIP

Assembly of functionally integrated human forebrain spheroids

(2017) Fikri Birey et al.   NATURE

Maintenance of neural progenitor cell stemness in 3D hydrogels requires matrix remodelling

(2017) Christopher M. Madl et al.   NATURE MATERIALS

Diverse Requirements for Microglial Survival, Specification, and Function Revealed by Defined-Medium Cultures

(2017) Christopher J. Bohlen et al.   NEURON

Microphysiological Human Brain and Neural Systems-on-a-Chip: Potential Alternatives to Small Animal Models and Emerging Platforms for Drug Discovery and Personalized Medicine

(2017) Alexander P. Haring et al.   Stem Cell Reviews and Reports

The Role of Astrocyte Dysfunction in Parkinson’s Disease Pathogenesis

(2017) Heather D.E. Booth et al.   TRENDS IN NEUROSCIENCES

A human brain microphysiological system derived from induced pluripotent stem cells to study neurological diseases and toxicity

(2017) David Pamies   ALTEX-Alternatives to Animal Experimentation

A neuroprotective astrocyte state is induced by neuronal signal EphB1 but fails in ALS models

(2017) Giulia E. Tyzack et al.   Nature Communications

The soft mechanical signature of glial scars in the central nervous system

(2017) Emad Moeendarbary et al.   Nature Communications

Microphysiological Human Brain and Neural Systems-on-a-Chip: Potential Alternatives to Small Animal Models and Emerging Platforms for Drug Discovery and Personalized Medicine

(2017) Alexander P. Haring et al.   Stem Cell Reviews and Reports

Systematic Three-Dimensional Coculture Rapidly Recapitulates Interactions between Human Neurons and Astrocytes

(2017) Robert Krencik et al.   Stem Cell Reports

Oligodendrocyte Precursor Cell Viability, Proliferation, and Morphology is Dependent on Mesh Size and Storage Modulus in 3D Poly(ethylene glycol)-Based Hydrogels

(2017) Lauren N. Russell et al.   ACS Biomaterials-Science & Engineering

Oligodendrocyte Precursor Cell Viability, Proliferation, and Morphology is Dependent on Mesh Size and Storage Modulus in 3D Poly(ethylene glycol)-Based Hydrogels

(2017) Lauren N. Russell et al.   ACS Biomaterials Science & Engineering

Flow Shear Stress Enhances the Proliferative Potential of Cultured Radial Glial Cells Possibly Via an Activation of Mechanosensitive Calcium Channel

(2017) Min Gu Park et al.   Experimental Neurobiology

ECM hydrogel for the treatment of stroke: Characterization of the host cell infiltrate

(2016) Harmanvir Ghuman et al.   BIOMATERIALS

Recent advances in hyaluronic acid hydrogels for biomedical applications

(2016) Christopher B Highley et al.   CURRENT OPINION IN BIOTECHNOLOGY

Development-on-chip:in vitroneural tube patterning with a microfluidic device

(2016) Christopher J. Demers et al.   DEVELOPMENT

Generation of highly enriched V2a interneurons from mouse embryonic stem cells

(2016) Nisha R. Iyer et al.   EXPERIMENTAL NEUROLOGY

Interstitial flow differentially increases patient-derived glioblastoma stem cell invasion via CXCR4, CXCL12, and CD44-mediated mechanisms

(2016) Kathryn M. Kingsmore et al.   Integrative Biology

Blood–brain barrier, bulk flow, and interstitial clearance in epilepsy

(2016) Nicola Marchi et al.   JOURNAL OF NEUROSCIENCE METHODS

Brain-on-a-chip model enables analysis of human neuronal differentiation and chemotaxis

(2016) Onur Kilic et al.   LAB ON A CHIP

Sex differences in astrocyte and microglia responses immediately following middle cerebral artery occlusion in adult mice

(2016) Helena W. Morrison et al.   NEUROSCIENCE

Modeling Ischemic Stroke In Vitro: Status Quo and Future Perspectives

(2016) Paul M. Holloway et al.   STROKE

Generating Diverse Spinal Motor Neuron Subtypes from Human Pluripotent Stem Cells

(2016) Rickie Patani   Stem Cells International

Regional brain stiffness changes across the Alzheimer's disease spectrum

(2016) Matthew C. Murphy et al.   NeuroImage-Clinical

Strain and rate-dependent neuronal injury in a 3D in vitro compression model of traumatic brain injury

(2016) Eyal Bar-Kochba et al.   Scientific Reports

High-throughput compound evaluation on 3D networks of neurons and glia in a microfluidic platform

(2016) Nienke R. Wevers et al.   Scientific Reports

Engineering Controllable Architecture in Matrigel for 3D Cell Alignment

(2015) Jae Myung Jang et al.   ACS Applied Materials & Interfaces

Solubilized extracellular matrix from brain and urinary bladder elicits distinct functional and phenotypic responses in macrophages

(2015) Fan Wei Meng et al.   BIOMATERIALS

3D printing of layered brain-like structures using peptide modified gellan gum substrates

(2015) Rodrigo Lozano et al.   BIOMATERIALS

Microfluidic device to control interstitial flow-mediated homotypic and heterotypic cellular communication

(2015) Luis F. Alonzo et al.   LAB ON A CHIP

Three-dimensional brain-on-a-chip with an interstitial level of flow and its application as an in vitro model of Alzheimer's disease

(2015) JiSoo Park et al.   LAB ON A CHIP

Organotypic Hippocampal Slices as Models for Stroke and Traumatic Brain Injury

(2015) Qian Li et al.   MOLECULAR NEUROBIOLOGY

Generation and isolation of oligodendrocyte progenitor cells from human pluripotent stem cells

(2015) Panagiotis Douvaras et al.   Nature Protocols

Measuring the effects of aging and sex on regional brain stiffness with MR elastography in healthy older adults

(2015) Arvin Arani et al.   NEUROIMAGE

3D in vitro modeling of the central nervous system

(2015) Amy M. Hopkins et al.   PROGRESS IN NEUROBIOLOGY

Engineering-Aligned 3D Neural Circuit in Microfluidic Device

(2015) Seokyoung Bang et al.   Advanced Healthcare Materials

Direct Conversion of Fibroblasts into Functional Astrocytes by Defined Transcription Factors

(2015) Massimiliano Caiazzo et al.   Stem Cell Reports

High and Low Molecular Weight Hyaluronic Acid Differentially Influence Macrophage Activation

(2015) Jamie E. Rayahin et al.   ACS Biomaterials-Science & Engineering

High and Low Molecular Weight Hyaluronic Acid Differentially Influence Macrophage Activation

(2015) Jamie E. Rayahin et al.   ACS Biomaterials Science & Engineering

Large-scale generation of human iPSC-derived neural stem cells/early neural progenitor cells and their neuronal differentiation

(2015) Leonardo D’Aiuto et al.   Organogenesis

S100β as an early biomarker of excitotoxic damage in spinal cord organotypic cultures

(2014) Graciela L. Mazzone et al.   JOURNAL OF NEUROCHEMISTRY

3D bioprinting of tissues and organs

(2014) Sean V Murphy et al.   NATURE BIOTECHNOLOGY

In Vivo Direct Reprogramming of Reactive Glial Cells into Functional Neurons after Brain Injury and in an Alzheimer’s Disease Model

(2013) Ziyuan Guo et al.   Cell Stem Cell

Oligodendrocyte-microglia cross-talk in the central nervous system

(2013) Laura Peferoen et al.   IMMUNOLOGY

Brain-wide pathway for waste clearance captured by contrast-enhanced MRI

(2013) Jeffrey J. Iliff et al.   JOURNAL OF CLINICAL INVESTIGATION

Acellular spinal cord scaffold seeded with bone marrow stromal cells protects tissue and promotes functional recovery in spinal cord-injured rats

(2013) Jian Chen et al.   JOURNAL OF NEUROSCIENCE RESEARCH

Adaptation of Endothelial Cells to Physiologically-Modeled, Variable Shear Stress

(2013) Joseph S. Uzarski et al.   PLoS One

A 3D in vitro model reveals differences in the astrocyte response elicited by potential stem cell therapies for CNS injury

(2013) Emma East et al.   Regenerative Medicine

Origin and differentiation of microglia

(2013) Florent Ginhoux et al.   Frontiers in Cellular Neuroscience

Biologic scaffolds composed of central nervous system extracellular matrix

(2012) Peter M. Crapo et al.   BIOMATERIALS

Hydrogels derived from central nervous system extracellular matrix

(2012) Christopher J. Medberry et al.   BIOMATERIALS

Interstitial Flow in a 3D Microenvironment Increases Glioma Invasion by a CXCR4-Dependent Mechanism

(2012) J. M. Munson et al.   CANCER RESEARCH

Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues

(2012) Jordan S. Miller et al.   NATURE MATERIALS

A Novel Porcine In Vitro Model of the Blood-Cerebrospinal Fluid Barrier with Strong Barrier Function

(2012) Mira Schroten et al.   PLoS One

A Paravascular Pathway Facilitates CSF Flow Through the Brain Parenchyma and the Clearance of Interstitial Solutes, Including Amyloid  

(2012) J. J. Iliff et al.   Science Translational Medicine

High molecular weight hyaluronic acid limits astrocyte activation and scar formation after spinal cord injury

(2011) Zin Z Khaing et al.   Journal of Neural Engineering

Decellularized Porcine Brain Matrix for Cell Culture and Tissue Engineering Scaffolds

(2011) Jessica A. DeQuach et al.   TISSUE ENGINEERING PART A

Direct conversion of fibroblasts to functional neurons by defined factors

(2010) Thomas Vierbuchen et al.   NATURE

Minireview: A Tiny Touch: Activation of Cell Signaling Pathways with Magnetic Nanoparticles

(2009) Nathan J. Sniadecki   ENDOCRINOLOGY

Vulnerability of glial cells to hydrogen peroxide in cultured hippocampal slices

(2008) Chris J. Feeney et al.   BRAIN RESEARCH

In Vitro and In Vivo Pharmacological Models to Assess Demyelination and Remyelination

(2008) Jean E Merrill   NEUROPSYCHOPHARMACOLOGY