Navigating across multi-dimensional space of tissue clearing parameters

Tissue clearing and its applications in neuroscience

(2020) Hiroki R. Ueda et al.   NATURE REVIEWS NEUROSCIENCE

Visualization and molecular characterization of whole-brain vascular networks with capillary resolution

(2020) Takeyuki Miyawaki et al.   Nature Communications

MACS: Rapid Aqueous Clearing System for 3D Mapping of Intact Organs

(2020) Jingtan Zhu et al.   Advanced Science

Versatile whole-organ/body staining and imaging based on electrolyte-gel properties of biological tissues

(2020) Etsuo A. Susaki et al.   Nature Communications

A versatile depigmentation, clearing, and labeling method for exploring nervous system diversity

(2020) Marko Pende et al.   Science Advances

New technical approaches for 3D morphological imaging and quantification of measurements

(2020) Cinzia Brenna et al.   Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology

C-ECi: A cubic-ECi combined clearing method for 3D follicular content analysis in The fish ovary†

(2020) Manon Lesage et al.   BIOLOGY OF REPRODUCTION

Biomedical applications of tissue clearing and three-dimensional imaging in health and disease

(2020) Maria Victoria Gómez-Gaviro et al.   iScience

Tissue clearing technique: Recent progress and biomedical applications

(2020) Ting Tian et al.   JOURNAL OF ANATOMY

Broad applicability of a streamlined Ethyl Cinnamate-based clearing procedure

(2019) Wouter Masselink et al.   DEVELOPMENT

A cationic near infrared fluorescent agent and ethyl-cinnamate tissue clearing protocol for vascular staining and imaging

(2019) Jiaguo Huang et al.   Scientific Reports

A Novel Optical Tissue Clearing Protocol for Mouse Skeletal Muscle to Visualize Endplates in Their Tissue Context

(2019) Marion Patrick Ivey Williams et al.   Frontiers in Cellular Neuroscience

High‐resolution imaging of fluorescent whole mouse brains using stabilised organic media (sDISCO)

(2019) Christian Hahn et al.   Journal of Biophotonics

High-dimensional cell-level analysis of tissues with Ce3D multiplex volume imaging

(2019) Weizhe Li et al.   Nature Protocols

Ultrafast optical clearing method for three-dimensional imaging with cellular resolution

(2019) Xinpei Zhu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Optical clearing methods: An overview of the techniques used for the imaging of 3D spheroids

(2019) Elisabete C. Costa et al.   BIOTECHNOLOGY AND BIOENGINEERING

Optical clearing potential of immersion-based agents applied to thick mouse brain sections

(2019) Mathew Loren et al.   PLoS One

Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues

(2019) Adam K. Glaser et al.   Nature Communications

In-vivo and ex-vivo optical clearing methods for biological tissues: review

(2019) Irene Costantini et al.   Biomedical Optics Express

Advanced CUBIC tissue clearing for whole-organ cell profiling

(2019) Katsuhiko Matsumoto et al.   Nature Protocols

A Clearing Technique to Enhance Endogenous Fluorophores in Skin and Soft Tissue

(2019) Deshka S. Foster et al.   Scientific Reports

Two simple criteria to estimate an objective’s performance when imaging in non design tissue clearing solutions

(2019) Sabrina Asteriti et al.   JOURNAL OF NEUROSCIENCE METHODS

Tissue clearing of both hard and soft tissue organs with the PEGASOS method

(2018) Dian Jing et al.   CELL RESEARCH

Optimized perfusion-based CUBIC protocol for the efficient whole-body clearing and imaging of rat organs

(2018) Paweł Matryba et al.   Journal of Biophotonics

Rapid spheroid clearing on a microfluidic chip

(2018) Tomas Silva Santisteban et al.   LAB ON A CHIP

A three-dimensional single-cell-resolution whole-brain atlas using CUBIC-X expansion microscopy and tissue clearing

(2018) Tatsuya C. Murakami et al.   NATURE NEUROSCIENCE

Three-dimensional intact-tissue sequencing of single-cell transcriptional states

(2018) Xiao Wang et al.   SCIENCE

Imaging transparent intact cardiac tissue with single-cell resolution

(2018) Zhiwei Wang et al.   Biomedical Optics Express

Multiplexed imaging of high-density libraries of RNAs with MERFISH and expansion microscopy

(2018) Guiping Wang et al.   Scientific Reports

RTF: a rapid and versatile tissue optical clearing method

(2018) Tingting Yu et al.   Scientific Reports

Optimizing tissue-clearing conditions based on analysis of the critical factors affecting tissue-clearing procedures

(2018) June Hoan Kim et al.   Scientific Reports

Multidirectional digital scanned light-sheet microscopy enables uniform fluorescence excitation and contrast-enhanced imaging

(2018) Adam K. Glaser et al.   Scientific Reports

Chemical Landscape for Tissue Clearing Based on Hydrophilic Reagents

(2018) Kazuki Tainaka et al.   Cell Reports

Evaluation of seven optical clearing methods in mouse brain

(2018) Peng Wan et al.   Neurophotonics

Quantitative assessment of optical clearing methods in various intact mouse organs

(2018) Jianyi Xu et al.   Journal of Biophotonics

Protection of tissue physicochemical properties using polyfunctional crosslinkers

(2018) Young-Gyun Park et al.   NATURE BIOTECHNOLOGY

Panoptic imaging of transparent mice reveals whole-body neuronal projections and skull–meninges connections

(2018) Ruiyao Cai et al.   NATURE NEUROSCIENCE

Optical clearing for multiscale biological tissues

(2017) Tingting Yu et al.   Journal of Biophotonics

Bone CLARITY: Clearing, imaging, and computational analysis of osteoprogenitors within intact bone marrow

(2017) Alon Greenbaum et al.   Science Translational Medicine

Elevated-temperature-induced acceleration of PACT clearing process of mouse brain tissue

(2017) Tingting Yu et al.   Scientific Reports

Whole-Body Profiling of Cancer Metastasis with Single-Cell Resolution

(2017) Shimpei I. Kubota et al.   Cell Reports

ePro-ClearSee: a simple immunohistochemical method that does not require sectioning of plant samples

(2017) Kiyotaka Nagaki et al.   Scientific Reports

UbasM: An effective balanced optical clearing method for intact biomedical imaging

(2017) Lingling Chen et al.   Scientific Reports

Exploring Passive Clearing for 3D Optical Imaging of Nanoparticles in Intact Tissues

(2016) Shrey Sindhwani et al.   BIOCONJUGATE CHEMISTRY

Single-molecule RNA detection at depth by hybridization chain reaction and tissue hydrogel embedding and clearing

(2016) Sheel Shah et al.   DEVELOPMENT

Comparison of different tissue clearing methods and 3D imaging techniques for visualization of GFP-expressing mouse embryos and embryonic hearts

(2016) Hana Kolesová et al.   HISTOCHEMISTRY AND CELL BIOLOGY

Fully Automated Evaluation of Total Glomerular Number and Capillary Tuft Size in Nephritic Kidneys Using Lightsheet Microscopy

(2016) Anika Klingberg et al.   JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY

Shrinkage-mediated imaging of entire organs and organisms using uDISCO

(2016) Chenchen Pan et al.   NATURE METHODS

High-performance multiplexed fluorescence in situ hybridization in culture and tissue with matrix imprinting and clearing

(2016) Jeffrey R. Moffitt et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Clarifying intact 3D tissues on a microfluidic chip for high-throughput structural analysis

(2016) Yih Yang Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

ACT-PRESTO: Rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging

(2016) Eunsoo Lee et al.   Scientific Reports

Simple, Scalable Proteomic Imaging for High-Dimensional Profiling of Intact Systems

(2015) Evan Murray et al.   CELL

Clarifying Tissue Clearing

(2015) Douglas S. Richardson et al.   CELL

ClearSee: a rapid optical clearing reagent for whole-plant fluorescence imaging

(2015) D. Kurihara et al.   DEVELOPMENT

Optical properties of mouse brain tissue after optical clearing with FocusClear™

(2015) Austin J. Moy et al.   JOURNAL OF BIOMEDICAL OPTICS

ScaleS: an optical clearing palette for biological imaging

(2015) Hiroshi Hama et al.   NATURE NEUROSCIENCE

Advanced CUBIC protocols for whole-brain and whole-body clearing and imaging

(2015) Etsuo A Susaki et al.   Nature Protocols

Stochastic electrotransport selectively enhances the transport of highly electromobile molecules

(2015) Sung-Yon Kim et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Expansion microscopy

(2015) F. Chen et al.   SCIENCE

Scalable and DiI-compatible optical clearance of the mammalian brain

(2015) Bing Hou et al.   Frontiers in Neuroanatomy

A versatile clearing agent for multi-modal brain imaging

(2015) Irene Costantini et al.   Scientific Reports

Fluorescent-Protein Stabilization and High-Resolution Imaging of Cleared, Intact Mouse Brains

(2015) Martin K. Schwarz et al.   PLoS One

Whole-Brain Imaging with Single-Cell Resolution Using Chemical Cocktails and Computational Analysis

(2014) Etsuo A. Susaki et al.   CELL

Single-Cell Phenotyping within Transparent Intact Tissue through Whole-Body Clearing

(2014) Bin Yang et al.   CELL

iDISCO: A Simple, Rapid Method to Immunolabel Large Tissue Samples for Volume Imaging

(2014) Nicolas Renier et al.   CELL

Whole-Body Imaging with Single-Cell Resolution by Tissue Decolorization

(2014) Kazuki Tainaka et al.   CELL

Genetic targeting of chemical indicators in vivo

(2014) Guoying Yang et al.   NATURE METHODS

ClearT: a detergent- and solvent-free clearing method for neuronal and non-neuronal tissue

(2013) T. Kuwajima et al.   DEVELOPMENT

Structural and molecular interrogation of intact biological systems

(2013) Kwanghun Chung et al.   NATURE

SeeDB: a simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction

(2013) Meng-Tsen Ke et al.   NATURE NEUROSCIENCE

Optical Histology: A Method to Visualize Microvasculature in Thick Tissue Sections of Mouse Brain

(2013) Austin J. Moy et al.   PLoS One

Chemical Clearing and Dehydration of GFP Expressing Mouse Brains

(2012) Klaus Becker et al.   PLoS One

Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain

(2011) Hiroshi Hama et al.   NATURE NEUROSCIENCE

Microtome-Free 3-Dimensional Confocal Imaging Method for Visualization of Mouse Intestine With Subcellular-Level Resolution

(2009) Ya–Yuan Fu et al.   GASTROENTEROLOGY