Article
Chemistry, Physical
Pengli Gao, Hui Hui, Chanjuan Guo, Yu Liu, Ya Su, Xiazi Huang, Kunxiong Guo, Wenting Shang, Jingying Jiang, Jie Tian
Summary: Successful preparation of NIR-CDs for real-time renal imaging was achieved, demonstrating high-efficiency renal clearance and reversible clearance in mice models, offering a new tool for imaging analysis of impaired kidney function.
Article
Oncology
Dongxin Liang, Xiaoqian Tuo, Qing Wang, Lanbo Zhao, Kailu Zhang, Yiran Wang, Xue Feng, Panyue Yin, Lin Guo, Yu Liu, Lei Wang, Lu Han, Ruifang An, Qiling Li
Summary: The study focused on synthesizing a new fluorescent agent for screening of endometrial cancer and precancerous change, which showed promising results in clinical validation, indicating that ZW-FA could be an effective cytologic strategy for in vitro diagnosis.
FRONTIERS IN ONCOLOGY
(2021)
Article
Chemistry, Analytical
Chao Jiang, Shining Li, Chuang Liu, Ruiyuan Liu, Jinqing Qu
Summary: A NIR cationic fluorescent probe PCTP with D-pi-A structure and large Stokes shift was designed and synthesized, which can differentiate RNA from DNA with high selectivity and sensitivity. The probe shows potential applications in RNA imaging and long-term cellular tracing.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Julia Ackermann, Jan Stegemann, Tim Smola, Eline Reger, Sebastian Jung, Anne Schmitz, Svenja Herbertz, Luise Erpenbeck, Karsten Seidl, Sebastian Kruss
Summary: Optical nanosensors based on single-wall carbon nanotubes are able to capture the dynamics of biochemical processes. A one-step phase separation approach has been developed to isolate monochiral (6,4)-SWCNTs from mixed SWCNT samples, enabling high-resolution imaging in the near-infrared spectrum using standard Si-based cameras. (6,4)-SWCNTs modified with (GT)(10)-ssDNA become highly sensitive to the neurotransmitter dopamine, allowing for fast and high-resolution imaging of dopamine release from cells.
Article
Chemistry, Analytical
Cynthia L. Schreiber, Dong-Hao Li, Bradley D. Smith
Summary: The study demonstrated that antibodies labeled with NIR heptamethine cyanine dye s775z outperformed commercially available NIR fluorophores in terms of brightness, photostability, and target specificity, making them highly suitable for sensitive NIR fluorescence detection and photon-intense methods.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Laura Moya-Anderico, Joana Admella, Eduard Torrents
Summary: This study presents an optimized and novel protocol for clearing Galleria mellonella larvae, allowing for visualization of internalized fluorescent nanoparticles using confocal microscopy and opening doors to a wide range of applications.
Article
Biochemistry & Molecular Biology
Sakkarapalayam M. Mahalingam, Karson S. Putt, Madduri Srinivasarao, Philip S. Low
Summary: The inadvertent severing of a ureter during colorectal surgeries is a common issue, leading to the development of new near-infrared dyes to help surgeons identify ureter location. The new PEGylated UreterGlow derivatives show promising optical and biodistribution characteristics, making them good candidates for translation into the clinic.
Article
Chemistry, Applied
Javier M. Abbas, Martin Stortz, Hernan B. Rodriguez, Valeria Levi, Alejandro Wolosiuk, Carla C. Spagnuolo
Summary: In this study, three novel near-infrared aminotricarbocyanine derivatives with antioxidant moieties gallic acid and Trolox were synthesized and investigated for their photophysical properties. The dyes showed enhanced photostability in aqueous and non-aqueous environments due to a self-healing process through redox quenching of reactive intermediates from excited states. Additionally, a functional group (-COOH) was included in the chemical structure of the dyes for potential conjugation with biomolecules or surfaces.
Review
Chemistry, Inorganic & Nuclear
Ya-Lin Qi, Yun-Zhan Li, Ming-Jun Tan, Fang-Fang Yuan, Niren Murthy, Yong-Tao Duan, Hai-Liang Zhu, Sheng-Yu Yang
Summary: In recent years, there has been great interest in the development of organic near-infrared (NIR) ratiometric fluorescent probes. Traditional intensity-based probes lack an internal standard method, resulting in unreliable and inaccurate detection signals as well as an inability to achieve quantitative determination of analytes. Smart dual emission probes with built-in correction ability have overcome the limitations of single channel fluorescent probes and enable reliable quantitative image analysis. Moreover, fluorescent chemosensors in the NIR window offer advantages such as high signal-to-noise ratio, low tissue damage, and deep tissue penetration. The combination of the advantages of ratiometric fluorescent probes and single-intensity-based NIR probes in NIR ratiometric fluorescent probes has led to their widespread application in biomedical research and clinical practice. This review provides a systematic summary of the recent advances (2020-2022) in this field, discussing the design strategies, sensing mechanisms, and bioapplications of these intelligent fluorescent probes. The challenges and prospects in this promising field are also addressed, with the anticipation that this work will inspire the development of organic NIR ratiometric fluorescent probes.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Chemistry, Physical
Yixiao Li, Shaohua Qu, Yumeng Xue, Lianbing Zhang, Li Shang
Summary: A thorough understanding of antimicrobial mechanism is crucial for developing novel antibacterial agents. In this study, cationic bovine serum albumin-protected AuAgNCs were synthesized and their antimicrobial process was investigated in real-time using fluorescence imaging. The cBSA-AuAgNCs exerted their antimicrobial effects by attaching on the outer membrane of bacteria without obvious internalization. Mechanistically, the cationic NCs caused disruption to the bacterial membrane due to strong electrostatic interactions, leading to the accumulation of reactive oxygen species (ROS) and eventually bactericidal action.
Article
Radiology, Nuclear Medicine & Medical Imaging
Hiroshi Fushiki, Tomoaki Yoshikawa, Toshihiro Matsuda, Takeshi Sato, Akira Suwa
Summary: This study investigated the use of a new fluorescence imaging technique for visualizing the ureter during surgery. The results showed that the technique was able to reliably visualize the ureter, and a dose of 0.01 mg/kg was found to be optimal for clinical use. This technology holds promise for reducing the risk of iatrogenic ureteral injury.
MOLECULAR IMAGING AND BIOLOGY
(2023)
Article
Chemistry, Analytical
Chao Zhao, Fangyuan Zhou, Kai Lu, Shikui Yang, Baojin Tan, Wanlu Sun, Lina Shangguan, Hai-Yan Wang, Yi Liu
Summary: The study designed and synthesized a novel near-infrared fluorescent probe to detect AChE activity, showing high specificity and sensitivity, as well as good biocompatibility both in vitro and in vivo. The probe provides a rapid and visual strategy for monitoring AChE activity.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Chemistry, Inorganic & Nuclear
Caiping Ding, Tianbing Ren
Summary: Near infrared (NIR) fluorescent probes have advantageous features including low background interference, strong tissue penetration, and minimal tissue damage. This paper provides a summary and discussion on the various types and properties of fluorophores used in the design of NIR fluorescent probes. It also discusses the latest research achievements in detecting and imaging reactive oxygen species, reactive nitrogen, and active sulfur in cells or in vivo. Additionally, a concise overview of the future development of NIR molecular fluorescence probes is proposed based on existing research, offering a reference for current research directions.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Pharmacology & Pharmacy
Gayoung Jo, Yoonbin Park, Min Ho Park, Hoon Hyun
Summary: A bone-targeted near-infrared fluorophore was developed for tumor-targeted imaging and photothermal therapy. The fluorescent probe exhibited excellent optical and photothermal properties, and could bind to hydroxyapatite, the main mineral component of bones. The nanoparticle-based probe showed high tumor targetability and effective photothermal treatment for cancer.
Review
Chemistry, Inorganic & Nuclear
Chenxu Yan, Yutao Zhang, Zhiqian Guo
Summary: Recent advances in molecularly NIR fluorescent probes for nanotheranostics have attracted increasing attention due to their potential in bridging in vivo imaging and therapeutic capability within a unimolecular system. The unique advantages of near-infrared (NIR) fluorescence imaging, such as low background, deep penetration, and minimal tissue damage, make it a promising tool for in vivo chemotherapy and phototherapy. The focus is on design strategy and functionalization of high-performance fluorophores, with discussions on current challenges and future prospects in bringing forth clinical applications for personalized treatment.
COORDINATION CHEMISTRY REVIEWS
(2021)
Article
Cell Biology
Maximilian E. A. Schaefer, Florian Keller, Jens Schumacher, Heinrich Haas, Fulvia Vascotto, Ugur Sahin, Mathias Hafner, Ruediger Rudolf
Summary: This study investigates the delivery of mRNA through lipoplex nanoparticles to melanoma spheroid cell culture models. The results demonstrate that the distribution and transfection efficiency of mRNA nanoparticles can be modulated by coculturing different cell types and through three-dimensional cell growth characteristics.
Review
Oncology
Aysel Ahadova, Johannes Witt, Saskia Haupt, Richard Gallon, Robert Hueneburg, Jacob Nattermann, Sanne Ten Broeke, Lena Bohaumilitzky, Alejandro Hernandez-Sanchez, Mauro Santibanez-Koref, Michael S. Jackson, Maarit Ahtiainen, Kirsi Pylvanainen, Katarina Andini, Vince Kornel Grolmusz, Gabriela Moeslein, Mev Dominguez-Valentin, Pal Moller, Daniel Fuerst, Rolf Sijmons, Gillian M. Borthwick, John Burn, Jukka-Pekka Mecklin, Vincent Heuveline, Magnus von Knebel Doeberitz, Toni Seppala, Matthias Kloor
Summary: Lynch syndrome is a common inherited cancer syndrome with a high risk of developing various malignancies. The HLA genotype of LS carriers plays an important role in the presentation of highly immunogenic antigens to the immune system and may influence cancer progression.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Cell Biology
Johannes Witt, Saskia Haupt, Aysel Ahadova, Lena Bohaumilitzky, Vera Fuchs, Alexej Ballhausen, Moritz Jakob Przybilla, Michael Jendrusch, Toni T. Seppala, Daniel Fuerst, Thomas Walle, Elena Busch, Georg Martin Haag, Robert Hueneburg, Jacob Nattermann, Magnus von Knebel Doeberitz, Vincent Heuveline, Matthias Kloor
Summary: The HLA system is an important component of antigen presentation machinery. Studying the relationship between HLA type and antigen presentation can improve our understanding of disease susceptibility. We have developed a refined approach to determine the presence or absence of HLA-A*02 in archival tissue samples.
Review
Cell Biology
Sarah Janice Hoerner, Nathalie Couturier, Daniele Caroline Gueiber, Mathias Hafner, Ruediger Rudolf
Summary: This article provides an overview of the current resources and methods for the differentiation of Schwann cells, supports the comparison and refinement of protocols, and aids the choice of suitable methods for specific applications.
Article
Biochemistry & Molecular Biology
Julian Schroeter, Tal Dattner, Jennifer Huellein, Alejandra Jayme, Vincent Heuveline, Georg F. Hoffmann, Stefan Koelker, Dominic Lenz, Thomas Opladen, Bernt Popp, Christian P. Schaaf, Christian Staufner, Steffen Syrbe, Sebastian Uhrig, Daniel Huebschmann, Heiko Brennenstuhl
Summary: The use of high-throughput sequencing techniques is producing a large number of disease-associated variants and candidate genes. Integration of genetic, protein, functional, and conservation knowledge is necessary to assess their relevance and prioritize further studies. However, accessing the required information from different databases can be challenging, leading to reduced accessibility for potential users. Here, we present a standalone webtool called aRgus, which allows simple extraction and intuitive visualization of multi-layered gene, protein, variant, and variant effect prediction data, enabling timely assessment of protein regions vulnerable to variation and providing applications in variant interpretation and experimental design.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Optics
Yaroslav Zharov, Evelina Ametova, Rebecca Spiecker, Tilo Baumbach, Genoveva Burca, Vincent Heuveline
Summary: This paper presents a method for improving the quality of noisy multi-channel imaging datasets by exploiting structural similarities between channels. The method draws pairs of samples from a data distribution with identical signals but uncorrelated noise. It is applicable to multi-channel datasets if adjacent channels provide images with similar enough information but independent noise. The authors demonstrate the applicability and performance of the method via three case studies.
Article
Multidisciplinary Sciences
Roman Bruch, Florian Keller, Moritz Boehland, Mario Vitacolonna, Lukas Klinger, Ruediger Rudolf, Markus Reischl
Summary: The analysis of 3D microscopic cell culture images is crucial for developing new therapeutics. Deep learning algorithms outperform conventional methods but require large amounts of training data. We propose a pipeline that combines simulation and deep learning optimization to generate synthetic 3D cell culture images with known labels.
Article
Biochemistry & Molecular Biology
Elaine Zaunseder, Ulrike Muetze, Sven F. Garbade, Saskia Haupt, Patrik Feyh, Georg F. Hoffmann, Vincent Heuveline, Stefan Koelker
Summary: Isovaleric aciduria (IVA) is a rare disorder of leucine metabolism that is included in newborn screening programs worldwide. However, the effectiveness of IVA screening is hindered by the identification of individuals with a milder variant of the disease and an increasing number of false positive results. In this study, machine learning methods were used to improve the classification of IVA, resulting in a significant reduction in false positive rates while maintaining high sensitivity.
Article
Medicine, General & Internal
Mev Dominguez-Valentin, Saskia Haupt, Toni T. Seppaelae, Julian R. Sampson, Lone Sunde, Inge Bernstein, Mark A. Jenkins, Christoph Engel, Stefan Aretz, Maartje Nielsen, Gabriel Capella, Francesc Balaguer, Dafydd Gareth Evans, John Burn, Elke Holinski-Feder, Lucio Bertario, Bernardo Bonanni, Annika Lindblom, Zohar Levi, Finlay Macrae, Ingrid Winship, John-Paul Plazzer, Rolf Sijmons, Luigi Laghi, Adriana Della Valle, Karl Heinimann, Tadeusz Debniak, Robert Fruscio, Francisco Lopez-Koestner, Karin Alvarez-Valenzuela, Lor H. Katz, Ido Laish, Elez Vainer, Carlos Vaccaro, Dirce Maria Carraro, Kevin Monahan, Elizabeth Half, Aine Stakelum, Des Winter, Rory Kennelly, Nathan Gluck, Harsh Sheth, Naim Abu-Freha, Marc Greenblatt, Benedito Mauro Rossi, Mabel Bohorquez, Giulia Martina Cavestro, Leonardo S. Lino-Silva, Karoline Horisberger, Maria Grazia Tibiletti, Ivana do Nascimento, Huw Thomas, Norma Teresa Rossi, Leandro Apolinario da Silva, Attila Zarand, Juan Ruiz-Banobre, Vincent Heuveline, Jukka-Pekka Mecklin, Kirsi Pylvaenaeinen, Laura Renkonen-Sinisalo, Anna Lepistoe, Paeivi Peltomaeki, Christina Therkildsen, Mia Gebauer Madsen, Stefan Kobbelgaard Burgdorf, John L. Hopper, Aung Ko Win, Robert W. Haile, Noralane Lindor, Steven Gallinge, Loic Le Marchand, Polly A. Newcomb, Jane Figueiredo, Daniel D. Buchanan, Stephen N. Thibodeau, Magnus von Knebel Doeberitz, Markus Loeffler, Nils Rahner, Evelin Schroeck, Verena Steinke-Lange, Wolff Schmiegel, Deepak Vangala, Claudia Perne, Robert Hueneburg, Silke Redler, Reinhard Buettner, Jurgen Weitz, Marta Pineda, Nuria Duenas, Joan Brunet Vidal, Leticia Moreira, Ariadna Sanchez, Eivind Hovig, Sigve Nakken, Kate Green, Fiona Lalloo, James Hill, Emma Crosbie, Miriam Mints, Yael Goldberg, Douglas Tjandra, Sanne W. ten Broeke, Revital Kariv, Guy Rosner, Suresh H. Advani, Lidiya Thomas, Pankaj Shah, Mithun Shah, Florencia Neffa, Patricia Esperon, Walter Pavicic, Giovana Tardin Torrezan, Thiago Bassaneze, Claudia Alejandra Martin, Gabriela Moslein, Pal Moller
Summary: This study used the latest PLSD cohort to investigate the mortality rates and age of cancer diagnoses in carriers of pathogenic MMR variants. The results showed that non-colorectal Lynch syndrome cancers caused more deaths than colorectal cancers in these carriers. Reducing deaths from non-colorectal cancers is a crucial challenge for the medical care of Lynch syndrome.
Review
Biochemistry & Molecular Biology
Sarah Janice Hoerner, Nathalie Couturier, Mathias Hafner, Ruediger Rudolf
Summary: Neuromuscular cell culture models are essential for studying synapse formation and function, as well as neuromuscular disease mechanisms. Recent advancements in 3D culture technique and hiPSC technology have improved their ability to complement in vivo models. However, the inclusion of Schwann cells in these cultures has been overlooked. This article provides an overview of different types of neuromuscular cocultures and highlights the effects of Schwann cells, along with proposing future research directions.
BIOLOGICAL CHEMISTRY
(2023)
Letter
Gastroenterology & Hepatology
Aysel Ahadova, Albrecht Stenzinger, Toni Seppala, Robert Hueneburg, Matthias Kloor, Hendrik Blaeker
Article
Chemistry, Analytical
Steffen Manser, Shaun Keck, Mario Vitacolonna, Felix Wuehler, Ruediger Rudolf, Matthias Raedle
Summary: Advancements in imaging techniques and adaptive visualization tools allow researchers to gain detailed insights into cellular organization and function, particularly in the field of biomedical research involving 3D cell cultures. The use of a multi-modal light sheet microscope can provide complementary spatial and molecular information for greater understanding and potential implications for therapy and drug development. The microscope's components specifically target inherent molecule properties like Rayleigh and Stokes Raman scattering, facilitating marker-free imaging and minimizing external influences on samples.
Article
Multidisciplinary Sciences
Michael Neumaier, Sophie Giesler, Volker Ast, Mathis Roemer, Timo-Daniel Voss, Eileen Reinz, Victor Costina, Martin Schmelz, Elina Nuernberg, Stefanie Nittka, Aino-Maija Leppae, Ruediger Rudolf, Andreas Trumpp, Tina Fuchs
Summary: This report introduces a new class of innate immune cells, called VIREMs, which are myeloid cells unrelated to lymphocytes. The researchers found that B-VIREMs, a subtype of VIREMs, have the ability to genetically recombine and express antibody genes, similar to B lymphocytes. They also discovered that B-VIREMs circulate in the blood of healthy individuals and undergo clonal expansion in response to disease stimuli. Live-cell imaging models suggest that B-VIREMs load their own Fc receptors with endogenous antibodies during vesicle transport. The findings suggest that these innate immune cells have important antigen-specific tissue maintenance functions.
Article
Mathematics, Applied
Philipp Gerstner, Vincent Heuveline
Summary: This paper proposes a full discretization scheme for the instationary thermal-electro-hydrodynamic (TEHD) Boussinesq equations. The scheme combines an H-1-conformal finite element method for spatial discretization with a backward differentiation formula (BDF) for time stepping. A priori convergence rates are derived and special care is taken of modeling the DEP force. Numerical experiments are conducted to verify the obtained error estimates.
ESAIM-MATHEMATICAL MODELLING AND NUMERICAL ANALYSIS
(2023)
Article
Oncology
Pal Moller, Toni Seppala, James G. Dowty, Saskia Haupt, Mev Dominguez-Valentin, Lone Sunde, Inge Bernstein, Christoph Engel, Stefan Aretz, Maartje Nielsen, Gabriel Capella, Dafydd Gareth Evans, John Burn, Elke Holinski-Feder, Lucio Bertario, Bernardo Bonanni, Annika Lindblom, Zohar Levi, Finlay Macrae, Ingrid Winship, John-Paul Plazzer, Rolf Sijmons, Luigi Laghi, Adriana Della Valle, Karl Heinimann, Elizabeth Half, Francisco Lopez-Koestner, Karin Alvarez-Valenzuela, Rodney J. Scott, Lior Katz, Ido Laish, Elez Vainer, Carlos Alberto Vaccaro, Dirce Maria Carraro, Nathan Gluck, Naim Abu-Freha, Aine Stakelum, Rory Kennelly, Des Winter, Benedito Mauro Rossi, Marc Greenblatt, Mabel Bohorquez, Harsh Sheth, Maria Grazia Tibiletti, Leonardo S. Lino-Silva, Karoline Horisberger, Carmen Portenkirchner, Ivana Nascimento, Norma Teresa Rossi, Leandro Apolinario da Silva, Huw Thomas, Attila Zarand, Jukka-Pekka Mecklin, Kirsi Pylvanainen, Laura Renkonen-Sinisalo, Anna Lepisto, Paivi Peltomaki, Christina Therkildsen, Lars Joachim Lindberg, Ole Thorlacius-Ussing, Magnus von Knebel Doeberitz, Markus Loeffler, Nils Rahner, Verena Steinke-Lange, Wolff Schmiegel, Deepak Vangala, Claudia Perne, Robert Hueneburg, Aida Falcon de Vargas, Andrew Latchford, Anne-Marie Gerdes, Ann-Sofie Backman, Carmen Guillen-Ponce, Carrie Snyder, Charlotte K. Lautrup, David Amor, Edenir Palmero, Elena Stoffel, Floor Duijkers, Michael J. Hall, Heather Hampel, Heinric Williams, Henrik Okkels, Jan Lubinski, Jeanette Reece, Joanne Ngeow, Jose G. Guillem, Julie Arnold, Karin Wadt, Kevin Monahan, Leigha Senter, Lene J. Rasmussen, Liselotte P. van Hest, Luigi Ricciardiello, Maija R. J. Kohonen-Corish, Marjolijn J. L. Ligtenberg, Melissa Southey, Melyssa Aronson, Mohd N. Zahary, N. Jewel Samadder, Nicola Poplawski, Nicoline Hoogerbrugge, Patrick J. Morrison, Paul James, Grant Lee, Rakefet Chen-Shtoyerman, Ravindran Ankathil, Rish Pai, Robyn Ward, Susan Parry, Tadeusz Debniak, Thomas John, Thomas van Overeem Hansen, Trinidad Caldes, Tatsuro Yamaguchi, Veronica Barca-Tierno, Pilar Garre, Giulia Martina Cavestro, Juergen Weitz, Silke Redler, Reinhard Buettner, VincentZ Heuveline, John L. Hopper, Aung Ko Win, Noralane Lindor, Steven Gallinger, Loic Le Marchand, Polly A. Newcomb, Jane Figueiredo, Daniel D. Buchanan, Stephen N. Thibodeau, Sanne W. ten Broeke, Eivind Hovig, Sigve Nakken, Marta Pineda, Nuria Duenas, Joan Brunet, Kate Green, Fiona Lalloo, Katie Newton, Emma J. Crosbie, Miriam Mints, Douglas Tjandra, Florencia Neffa, Patricia Esperon, Revital Kariv, Guy Rosner, Walter Hernan Pavicic, Pablo Kalfayan, Giovana Tardin Torrezan, Thiago Bassaneze, Claudia Martin, Gabriela Moslein, Aysel Ahadova, Matthias Kloor, Julian R. Sampson, Mark A. Jenkins
Summary: This study compared the colorectal cancer incidence in carriers of pathogenic variants of the MMR genes in two cohorts. The PLSD cohort, with mandatory colonoscopy surveillance, had higher CRC incidences compared to the retrospective IMRC cohort. Surprisingly, the incidence was not reduced in path_MSH6 carriers. However, the incidence in path_PMS2 carriers was reduced, though not significantly.
HEREDITARY CANCER IN CLINICAL PRACTICE
(2022)
