Structural analyses of the PKA RIIβ holoenzyme containing the oncogenic DnaJB1-PKAc fusion protein reveal protomer asymmetry and fusion-induced allosteric perturbations in fibrolamellar hepatocellular carcinoma

Allosteric pluripotency as revealed by protein kinase A

(2020) J. A. Byun et al.   Science Advances

Structures of the PKA RIα Holoenzyme with the FLHCC Driver J-PKAcα or Wild-Type PKAcα

(2019) Baohua Cao et al.   STRUCTURE

Two PKA RIα holoenzyme states define ATP as an isoform-specific orthosteric inhibitor that competes with the allosteric activator, cAMP

(2019) Tsan-Wen Lu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Activation of PKA via asymmetric allosteric coupling of structurally conserved cyclic nucleotide binding domains

(2019) Yuxin Hao et al.   Nature Communications

New Opportunities Created by Single-Particle Cryo-EM: The Mapping of Conformational Space

(2018) Joachim Frank   BIOCHEMISTRY

Conformational Landscape of the PRKACA-DNAJB1 Chimeric Kinase, the Driver for Fibrolamellar Hepatocellular Carcinoma

(2018) Michael D. Tomasini et al.   Scientific Reports

Switching of the folding-energy landscape governs the allosteric activation of protein kinase A

(2018) Jeneffer P. England et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CRISPR/Cas9 Engineering of Adult Mouse Liver Demonstrates That the Dnajb1 – Prkaca Gene Fusion Is Sufficient to Induce Tumors Resembling Fibrolamellar Hepatocellular Carcinoma

(2017) Lars H. Engelholm et al.   GASTROENTEROLOGY

DNAJB1–PRKACAfusion kinase interacts with β-catenin and the liver regenerative response to drive fibrolamellar hepatocellular carcinoma

(2017) Edward R. Kastenhuber et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mechanisms for restraining cAMP-dependent protein kinase revealed by subunit quantitation and cross-linking approaches

(2017) Ryan Walker-Gray et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Liberated PKA Catalytic Subunits Associate with the Membrane via Myristoylation to Preferentially Phosphorylate Membrane Substrates

(2017) Shane E. Tillo et al.   Cell Reports

Isoform-specific subcellular localization and function of protein kinase A identified by mosaic imaging of mouse brain

(2017) Ronit Ilouz et al.   eLife

Structure of a PKA RIα Recurrent Acrodysostosis Mutant Explains Defective cAMP-Dependent Activation

(2016) Jessica GH Bruystens et al.   JOURNAL OF MOLECULAR BIOLOGY

Enhanced cAMP-stimulated protein kinase A activity in human fibrolamellar hepatocellular carcinoma

(2016) Kevin M. Riggle et al.   PEDIATRIC RESEARCH

I-TASSER server: new development for protein structure and function predictions

(2015) Jianyi Yang et al.   NUCLEIC ACIDS RESEARCH

Transcriptomic characterization of fibrolamellar hepatocellular carcinoma

(2015) Elana P. Simon et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural insights into mis-regulation of protein kinase A in human tumors

(2015) Jonah Cheung et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

An Isoform-Specific Myristylation Switch Targets Type II PKA Holoenzymes to Membranes

(2015) Ping Zhang et al.   STRUCTURE

Mapping the Free Energy Landscape of PKA Inhibition and Activation: A Double-Conformational Selection Model for the Tandem cAMP-Binding Domains of PKA RIα

(2015) Madoka Akimoto et al.   PLOS BIOLOGY

Isoform-Selective Disruption of AKAP-Localized PKA Using Hydrocarbon Stapled Peptides

(2014) Yuxiao Wang et al.   ACS Chemical Biology

Differentially Regulated Protein Kinase A (PKA) Activity in Adipose Tissue and Liver Is Associated With Resistance to Diet-Induced Obesity and Glucose Intolerance in Mice That Lack PKA Regulatory Subunit Type IIα

(2014) Edra London et al.   ENDOCRINOLOGY

Using Markov State Models to Develop a Mechanistic Understanding of Protein Kinase A Regulatory Subunit RIα Activation in Response to cAMP Binding

(2014) Britton W. Boras et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Dynamic architecture of a protein kinase

(2014) C. L. McClendon et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Detection of a Recurrent DNAJB1-PRKACA Chimeric Transcript in Fibrolamellar Hepatocellular Carcinoma

(2014) J. N. Honeyman et al.   SCIENCE

PKA: Lessons learned after twenty years

(2013) Susan S. Taylor et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Intrinsic disorder within an AKAP-protein kinase A complex guides local substrate phosphorylation

(2013) F Donelson Smith et al.   eLife

New developments in theATSASprogram package for small-angle scattering data analysis

(2012) Maxim V. Petoukhov et al.   JOURNAL OF APPLIED CRYSTALLOGRAPHY

Assembly of allosteric macromolecular switches: lessons from PKA

(2012) Susan S. Taylor et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Localization and quaternary structure of the PKA RI  holoenzyme

(2012) R. Ilouz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure and Allostery of the PKA RII  Tetrameric Holoenzyme

(2012) P. Zhang et al.   SCIENCE

Characterizing flexible and intrinsically unstructured biological macromolecules by SAS using the Porod-Debye law

(2011) Robert P. Rambo et al.   BIOPOLYMERS

Isoform-specific targeting of PKA to multivesicular bodies

(2011) Michele E. Day et al.   JOURNAL OF CELL BIOLOGY

A Myristoyl/Phosphoserine Switch Controls cAMP-Dependent Protein Kinase Association to Membranes

(2011) Ece C. Gaffarogullari et al.   JOURNAL OF MOLECULAR BIOLOGY

Communication between Tandem cAMP Binding Domains in the Regulatory Subunit of Protein Kinase A-Iα as Revealed by Domain-silencing Mutations

(2010) E. Tyler McNicholl et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Disruption of Protein Kinase A Localization Using a Trans-activator of Transcription (TAT)-conjugated A-kinase-anchoring Peptide Reduces Cardiac Function

(2010) Hemal H. Patel et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A conserved deamidation site at asn 2 in the catalytic subunit of mammalian cAMP-dependent protein kinase detected by capillary LC-MS and tandem mass spectrometry

(2010) Paul T. Jedrzejewski et al.   PROTEIN SCIENCE

Importance of the A-helix of the catalytic subunit of cAMP-dependent protein kinase for stability and for orienting subdomains at the cleft interface

(2010) Friedrich W. Herberg et al.   PROTEIN SCIENCE

Structure of D-AKAP2:PKA RI Complex: Insights into AKAP Specificity and Selectivity

(2010) Ganapathy N. Sarma et al.   STRUCTURE

Robust, high-throughput solution structural analyses by small angle X-ray scattering (SAXS)

(2009) Greg L Hura et al.   NATURE METHODS

A Generalized Allosteric Mechanism for cis-Regulated Cyclic Nucleotide Binding Domains

(2008) Alexandr P. Kornev et al.   PLoS Computational Biology