TORC1 Determines Fab1 Lipid Kinase Function at Signaling Endosomes and Vacuoles

The flipside of the TOR coin – TORC2 and plasma membrane homeostasis at a glance

(2020) Margot Riggi et al.   JOURNAL OF CELL SCIENCE

A spatially and functionally distinct pool of TORC1 defines signaling endosomes in yeast

(2019) Riko Hatakeyama et al.   Autophagy

TORC1 specifically inhibits microautophagy through ESCRT-0

(2019) Riko Hatakeyama et al.   CURRENT GENETICS

Interaction of the late endo-lysosomal lipid PI(3,5)P2 with the Vph1 isoform of yeast V-ATPase increases its activity and cellular stress tolerance

(2019) Subhrajit Banerjee et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Lysosome enlargement during inhibition of the lipid kinase PIKfyve proceeds through lysosome coalescence

(2018) Christopher H. Choy et al.   JOURNAL OF CELL SCIENCE

Vacuole-mediated selective regulation of TORC1-Sch9 signaling following oxidative stress

(2018) Eigo Takeda et al.   MOLECULAR BIOLOGY OF THE CELL

Control of vacuole membrane homeostasis by a resident PI-3,5-kinase inhibitor

(2018) PC Malia et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Gtr/Ego-independent TORC1 activation is achieved through a glutamine-sensitive interaction with Pib2 on the vacuolar membrane

(2018) Hirofumi Ukai et al.   PLoS Genetics

Ivy1 is a negative regulator of Gtr-dependent TORC1 activation

(2018) Natalia V. Varlakhanova et al.   JOURNAL OF CELL SCIENCE

Decrease in plasma membrane tension triggers PtdIns(4,5)P2 phase separation to inactivate TORC2

(2018) Margot Riggi et al.   NATURE CELL BIOLOGY

Multi-layered regulation of TORC1-body formation in budding yeast

(2018) Arron Sullivan et al.   MOLECULAR BIOLOGY OF THE CELL

Spatially Distinct Pools of TORC1 Balance Protein Homeostasis

(2018) Riko Hatakeyama et al.   MOLECULAR CELL

Early protection to stress mediated by CDK-dependent PI3,5P2signaling from the vacuole/lysosome

(2017) Natsuko Jin et al.   JOURNAL OF CELL BIOLOGY

Pib2 and the EGO complex are both required for activation of TORC1

(2017) Natalia V. Varlakhanova et al.   JOURNAL OF CELL SCIENCE

An In Vitro TORC1 Kinase Assay That Recapitulates the Gtr-Independent Glutamine-Responsive TORC1 Activation Mechanism on Yeast Vacuoles

(2017) Mirai Tanigawa et al.   MOLECULAR AND CELLULAR BIOLOGY

An intramolecular interaction within the lipid kinase Fab1 regulates cellular phosphatidylinositol 3,5-bisphosphate lipid levels

(2017) Michael J. Lang et al.   MOLECULAR BIOLOGY OF THE CELL

TORC1 organized in inhibited domains (TOROIDs) regulate TORC1 activity

(2017) Manoël Prouteau et al.   NATURE

Emerging Roles for the Lysosome in Lipid Metabolism

(2017) Ashley M. Thelen et al.   TRENDS IN CELL BIOLOGY

Feedback Inhibition of the Rag GTPase GAP Complex Lst4-Lst7 Safeguards TORC1 from Hyperactivation by Amino Acid Signals

(2017) Marie-Pierre Péli-Gulli et al.   Cell Reports

Recruitment dynamics of ESCRT-III and Vps4 to endosomes and implications for reverse membrane budding

(2017) Manuel Alonso Y Adell et al.   eLife

Functional mapping of yeast genomes by saturated transposition

(2017) Agnès H Michel et al.   eLife

Structure of the Tuberous Sclerosis Complex 2 (TSC2) N Terminus Provides Insight into Complex Assembly and Tuberous Sclerosis Pathogenesis

(2016) Reinhard Zech et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Simplified CRISPR-Cas genome editing for Saccharomyces cerevisiae

(2016) Wesley Cardoso Generoso et al.   JOURNAL OF MICROBIOLOGICAL METHODS

The Perseus computational platform for comprehensive analysis of (prote)omics data

(2016) Stefka Tyanova et al.   NATURE METHODS

The I-BAR protein Ivy1 is an effector of the Rab7 GTPase Ypt7 involved in vacuole membrane homeostasis

(2015) J. Numrich et al.   JOURNAL OF CELL SCIENCE

A LAPF/phafin1-like protein regulates TORC1 and lysosomal membrane permeabilization in response to endoplasmic reticulum membrane stress

(2015) Adam Kim et al.   MOLECULAR BIOLOGY OF THE CELL

Fast and easy phosphopeptide fractionation by combinatorial ERLIC-SCX solid-phase extraction for in-depth phosphoproteome analysis

(2015) Mostafa Zarei et al.   Nature Protocols

Phosphatidylinositol 3,5-Bisphosphate-Rich Membrane Domains in Endosomes and Lysosomes

(2015) Sho Takatori et al.   TRAFFIC

Nitrogen Source Activates TOR (Target of Rapamycin) Complex 1 via Glutamine and Independently of Gtr/Rag Proteins

(2014) Daniele Stracka et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Roles for PI(3,5)P2 in nutrient sensing through TORC1

(2014) Natsuko Jin et al.   MOLECULAR BIOLOGY OF THE CELL

The signaling lipid PI(3,5)P2 stabilizes V1–Vo sector interactions and activates the V-ATPase

(2014) Sheena Claire Li et al.   MOLECULAR BIOLOGY OF THE CELL

Retromer: A Master Conductor of Endosome Sorting

(2014) C. Burd et al.   Cold Spring Harbor Perspectives in Biology

Endocytosis, Signaling, and Beyond

(2014) P. P. Di Fiore et al.   Cold Spring Harbor Perspectives in Biology

Phosphatidylinositol 3,5-bisphosphate: Low abundance, high significance

(2013) Amber J. McCartney et al.   BIOESSAYS

SEACing the GAP that nEGOCiates TORC1 activation

(2013) Nicolas Panchaud et al.   CELL CYCLE

Vac14 Protein Multimerization Is a Prerequisite Step for Fab1 Protein Complex Assembly and Function

(2013) Tamadher A. Alghamdi et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Phosphoinositides: Tiny Lipids With Giant Impact on Cell Regulation

(2013) Tamas Balla   PHYSIOLOGICAL REVIEWS

A Tumor Suppressor Complex with GAP Activity for the Rag GTPases That Signal Amino Acid Sufficiency to mTORC1

(2013) L. Bar-Peled et al.   SCIENCE

Amino Acid Deprivation Inhibits TORC1 Through a GTPase-Activating Protein Complex for the Rag Family GTPase Gtr1

(2013) N. Panchaud et al.   Science Signaling

Phosphatidylinositol 3,5-bisphosphate plays a role in the activation and subcellular localization of mechanistic target of rapamycin 1

(2012) Dave Bridges et al.   MOLECULAR BIOLOGY OF THE CELL

Cell-free reconstitution of vacuole membrane fragmentation reveals regulation of vacuole size and number by TORC1

(2012) Lydie Michaillat et al.   MOLECULAR BIOLOGY OF THE CELL

In vivo, Pikfyve generates PI(3,5)P2, which serves as both a signaling lipid and the major precursor for PI5P

(2012) S. N. Zolov et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Endosome maturation

(2011) Jatta Huotari et al.   EMBO JOURNAL

Target of Rapamycin (TOR) in Nutrient Signaling and Growth Control

(2011) Robbie Loewith et al.   GENETICS

Phosphatidylinositol-3,5-Bisphosphate: No Longer the Poor PIP2

(2011) Cheuk Y. Ho et al.   TRAFFIC

Pathogenic Mechanism of the FIG4 Mutation Responsible for Charcot-Marie-Tooth Disease CMT4J

(2011) Guy M. Lenk et al.   PLoS Genetics

Genetic Interaction between MTMR2 and FIG4 Phospholipid Phosphatases Involved in Charcot-Marie-Tooth Neuropathies

(2011) Ilaria Vaccari et al.   PLoS Genetics

PI(3,5)P2 controls membrane trafficking by direct activation of mucolipin Ca2+ release channels in the endolysosome

(2010) Xian-ping Dong et al.   Nature Communications

Deleterious Variants of FIG4, a Phosphoinositide Phosphatase, in Patients with ALS

(2009) Clement Y. Chow et al.   AMERICAN JOURNAL OF HUMAN GENETICS

TORC2 Plasma Membrane Localization Is Essential for Cell Viability and Restricted to a Distinct Domain

(2009) Doris Berchtold et al.   MOLECULAR BIOLOGY OF THE CELL

The Vam6 GEF Controls TORC1 by Activating the EGO Complex

(2009) Matteo Binda et al.   MOLECULAR CELL

VAC14 nucleates a protein complex essential for the acute interconversion of PI3P and PI(3,5)P2 in yeast and mouse

(2008) Natsuko Jin et al.   EMBO JOURNAL

TOR1 and TOR2 Have Distinct Locations in Live Cells

(2008) Thomas W. Sturgill et al.   EUKARYOTIC CELL

Assembly of a Fab1 Phosphoinositide Kinase Signaling Complex Requires the Fig4 Phosphoinositide Phosphatase

(2008) Roberto J. Botelho et al.   MOLECULAR BIOLOGY OF THE CELL

MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification

(2008) Jürgen Cox et al.   NATURE BIOTECHNOLOGY