Tailoring recombinant protein quality by rational media design

Betanin reduces the accumulation and cross-links of collagen in high-fructose-fed rat heart through inhibiting non-enzymatic glycation

(2015) Junyan Han et al.   CHEMICO-BIOLOGICAL INTERACTIONS

Effect of glucose feeding on the glycosylation quality of antibody produced by a human cell line, F2N78, in fed-batch culture

(2014) Jin Seok Seo et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Effect of lithium chloride on the production and sialylation of Fc-fusion protein in Chinese hamster ovary cell culture

(2014) Tae Kwang Ha et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Use of a small molecule cell cycle inhibitor to control cell growth and improve specific productivity and product quality of recombinant proteins in CHO cell cultures

(2014) Zhimei Du et al.   BIOTECHNOLOGY AND BIOENGINEERING

Recent advances in the understanding of biological implications and modulation methodologies of monoclonal antibody N-linked high mannose glycans

(2014) Helen H. Shi et al.   BIOTECHNOLOGY AND BIOENGINEERING

Identification of manipulated variables for a glycosylation control strategy

(2014) Melissa M. St. Amand et al.   BIOTECHNOLOGY AND BIOENGINEERING

Control of galactosylated glycoforms distribution in cell culture system

(2014) Neil A. McCracken et al.   BIOTECHNOLOGY PROGRESS

Cell culture media supplementation of uncommonly used sugars sucrose and tagatose for the targeted shifting of protein glycosylation profiles of recombinant protein therapeutics

(2014) Patrick Hossler et al.   BIOTECHNOLOGY PROGRESS

Modulation of mAb quality attributes using microliter scale fed-batch cultures

(2014) Yolande Rouiller et al.   BIOTECHNOLOGY PROGRESS

Direct analysis of mAb aggregates in mammalian cell culture supernatant

(2014) Albert J Paul et al.   BMC BIOTECHNOLOGY

Total Synthesis and Glycosidase Inhibition Studies of (-)-Gabosine J and Its Derivatives

(2014) Adiyala Vidyasagar et al.   EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

Evaluating the impact of cell culture process parameters on monoclonal antibody N-glycosylation

(2014) Marija Ivarsson et al.   JOURNAL OF BIOTECHNOLOGY

Donor Assists Acceptor Binding and Catalysis of Human α1,6-Fucosyltransferase

(2013) Miriam P. Kötzler et al.   ACS Chemical Biology

Method to convert N-terminal glutamine to pyroglutamate for characterization of recombinant monoclonal antibodies

(2013) Wei Xu et al.   ANALYTICAL BIOCHEMISTRY

Investigation of the correlation between charge and glycosylation of IgG1 variants by liquid chromatography–mass spectrometry

(2013) Jia-Ming Yang et al.   ANALYTICAL BIOCHEMISTRY

Arginine Modifications by Methylglyoxal: Discovery in a Recombinant Monoclonal Antibody and Contribution to Acidic Species

(2013) Chris Chumsae et al.   ANALYTICAL CHEMISTRY

Combined Inhibitor Free-Energy Landscape and Structural Analysis Reports on the Mannosidase Conformational Coordinate

(2013) Rohan J. Williams et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Selective inhibition of glycosyltransferases by bivalent imidazolium salts

(2013) Yin Gao et al.   BIOORGANIC & MEDICINAL CHEMISTRY

CHO cell line specific prediction and control of recombinant monoclonal antibodyN-glycosylation

(2013) Rhian K. Grainger et al.   BIOTECHNOLOGY AND BIOENGINEERING

Free light chain content in culture media reflects recombinant monoclonal antibody productivity and quality

(2013) Prachi Bhoskar et al.   BIOTECHNOLOGY PROGRESS

Improvement of mammalian cell culture performance through surfactant enabled concentrated feed media

(2013) Patrick Hossler et al.   BIOTECHNOLOGY PROGRESS

Effect of cell culture medium components on color of formulated monoclonal antibody drug substance

(2013) Natarajan Vijayasankaran et al.   BIOTECHNOLOGY PROGRESS

Development of fucosyltransferase and fucosidase inhibitors

(2013) Zhijay Tu et al.   CHEMICAL SOCIETY REVIEWS

Glycosylation analysis of an aggregated antibody produced by Chinese hamster ovary cells in bioreactor culture

(2013) Masayoshi Onitsuka et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Trehalose suppresses antibody aggregation during the culture of Chinese hamster ovary cells

(2013) Masayoshi Onitsuka et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

The availability of glucose to CHO cells affects the intracellular lipid-linked oligosaccharide distribution, site occupancy and the N-glycosylation profile of a monoclonal antibody

(2013) Bo Liu et al.   JOURNAL OF BIOTECHNOLOGY

Asparagine Deamidation Dependence on Buffer Type, pH, and Temperature

(2013) Amanda L. Pace et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

A high-throughput media design approach for high performance mammalian fed-batch cultures

(2013) Yolande Rouiller et al.   mAbs

Physiochemical and Biochemical Factors Influencing the Pharmacokinetics of Antibody Therapeutics

(2012) Daniela Bumbaca et al.   AAPS Journal

Recent advances in technology supporting biopharmaceutical production from mammalian cells

(2012) M. Butler et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Probing of C-terminal lysine variation in a recombinant monoclonal antibody production using Chinese hamster ovary cells with chemically defined media

(2012) Jun Luo et al.   BIOTECHNOLOGY AND BIOENGINEERING

Effect of hydrocortisone on the production and glycosylation of an Fc-fusion protein in CHO cell cultures

(2012) Yolande Rouiller et al.   BIOTECHNOLOGY PROGRESS

Quality attributes of recombinant therapeutic proteins: An assessment of impact on safety and efficacy as part of a quality by design development approach

(2012) Alex Eon-Duval et al.   BIOTECHNOLOGY PROGRESS

Cell culture medium improvement by rigorous shuffling of components using media blending

(2012) Martin Jordan et al.   CYTOTECHNOLOGY

Rational development of a serum-free medium and fed-batch process for a GS-CHO cell line expressing recombinant antibody

(2012) Huifeng Zhang et al.   CYTOTECHNOLOGY

C-terminal modification of monoclonal antibody drugs: Amidated species as a general product-related substance

(2012) Masahiro Tsubaki et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Inhibition of Galactosyltransferases by a Novel Class of Donor Analogues

(2012) Karine Descroix et al.   JOURNAL OF MEDICINAL CHEMISTRY

Characterization of the basic charge variants of a human IgG1

(2012) Timothy Kaschak et al.   mAbs

Marketing approval of mogamulizumab

(2012) Alain Beck et al.   mAbs

Global metabolic inhibitors of sialyl- and fucosyltransferases remodel the glycome

(2012) Cory D Rillahan et al.   Nature Chemical Biology

Alpha-mannosidase activity in stallion epididymal fluid and spermatozoa

(2012) C.A. Retamal et al.   THERIOGENOLOGY

Mammalian cell protein expression for biopharmaceutical production

(2011) Jianwei Zhu   BIOTECHNOLOGY ADVANCES

Modulation of antibody galactosylation through feeding of uridine, manganese chloride, and galactose

(2011) Michael J. Gramer et al.   BIOTECHNOLOGY AND BIOENGINEERING

High-End pH-controlled delivery of glucose effectively suppresses lactate accumulation in CHO Fed-batch cultures

(2011) Matthew Gagnon et al.   BIOTECHNOLOGY AND BIOENGINEERING

Effects of cell culture conditions on antibody N-linked glycosylation-what affects high mannose 5 glycoform

(2011) Efren Pacis et al.   BIOTECHNOLOGY AND BIOENGINEERING

Aggregates in monoclonal antibody manufacturing processes

(2011) María Vázquez-Rey et al.   BIOTECHNOLOGY AND BIOENGINEERING

Controlling glycation of recombinant antibody in fed-batch cell cultures

(2011) Inn H. Yuk et al.   BIOTECHNOLOGY AND BIOENGINEERING

Towards dynamic metabolic flux analysis in CHO cell cultures

(2011) Woo Suk Ahn et al.   Biotechnology Journal

Synergizing metabolic flux analysis and nucleotide sugar metabolism to understand the control of glycosylation of recombinant protein in CHO cells

(2011) Susan C Burleigh et al.   BMC BIOTECHNOLOGY

Fucose content of monoclonal antibodies can be controlled by culture medium osmolality for high antibody-dependent cellular cytotoxicity

(2011) Yoshinobu Konno et al.   CYTOTECHNOLOGY

Calystegine B3 as a specific inhibitor for cytoplasmic  -mannosidase, Man2C1

(2011) A. Kato et al.   JOURNAL OF BIOCHEMISTRY

Effects of zinc and manganese on advanced glycation end products (AGEs) formation and AGEs-mediated endothelial cell dysfunction

(2011) Xiuyuan Zhuang et al.   LIFE SCIENCES

Fragmentation of monoclonal antibodies

(2011) Josef Vlasak et al.   mAbs

Acceptable changes in quality attributes of glycosylated biopharmaceuticals

(2011) Martin Schiestl et al.   NATURE BIOTECHNOLOGY

The first C-glycosidic analogue of a novel galactosyltransferase inhibitor

(2011) Karine Descroix et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Identification of cell culture conditions to control protein aggregation of IgG fusion proteins expressed in Chinese hamster ovary cells

(2011) Ying Jing et al.   PROCESS BIOCHEMISTRY

Inhibition of advanced glycation endproduct formation by foodstuffs

(2011) Chi-Hao Wu et al.   Food & Function

Effects of Charge on Antibody Tissue Distribution and Pharmacokinetics

(2010) C. Andrew Boswell et al.   BIOCONJUGATE CHEMISTRY

Mechanism of antibody reduction in cell culture production processes

(2010) Yung-Hsiang Kao et al.   BIOTECHNOLOGY AND BIOENGINEERING

Fragmentation of a highly purified monoclonal antibody attributed to residual CHO cell protease activity

(2010) Sharon X. Gao et al.   BIOTECHNOLOGY AND BIOENGINEERING

An investigation of intracellular glycosylation activities in CHO cells: Effects of nucleotide sugar precursor feeding

(2010) Niki S.C. Wong et al.   BIOTECHNOLOGY AND BIOENGINEERING

Control of misincorporation of serine for asparagine during antibody production using CHO cells

(2010) Anurag Khetan et al.   BIOTECHNOLOGY AND BIOENGINEERING

Understanding the intracellular effect of enhanced nutrient feeding toward high titer antibody production process

(2010) Marcella Yu et al.   BIOTECHNOLOGY AND BIOENGINEERING

Towards the implementation of quality by design to the production of therapeutic monoclonal antibodies with desired glycosylation patterns

(2010) Ioscani Jimenez del Val et al.   BIOTECHNOLOGY PROGRESS

Maximizing productivity of CHO cell-based fed-batch culture using chemically defined media conditions and typical manufacturing equipment

(2010) Yao-Ming Huang et al.   BIOTECHNOLOGY PROGRESS

Mass spectrometric analysis of asparagine deamidation and aspartate isomerization in polypeptides

(2010) Hongqian Yang et al.   ELECTROPHORESIS

Specificity of β1,4-galactosyltransferase inhibition by 2-naphthyl 2-butanamido-2-deoxy-1-thio-β-D-glucopyranoside

(2010) Yin Gao et al.   GLYCOCONJUGATE JOURNAL

An Efficient Approach to the Discovery of Potent Inhibitors against Glycosyltransferases

(2010) Kensaku Hosoguchi et al.   JOURNAL OF MEDICINAL CHEMISTRY

Comparative Evaluation of Disodium Edetate and Diethylenetriaminepentaacetic Acid as Iron Chelators to Prevent Metal -Catalyzed Destabilization of a Therapeutic Monoclonal Antibody

(2010) Shuxia Zhou et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Molecular Mechanism of the Glycosylation Step Catalyzed by Golgi α-Mannosidase II: A QM/MM Metadynamics Investigation

(2010) Luis Petersen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Cell culture processes for monoclonal antibody production

(2010) Feng Li et al.   mAbs

Charge variants in IgG1

(2010) Leslie A. Khawli et al.   mAbs

Mechanistic insights into a Ca2+-dependent family of α-mannosidases in a human gut symbiont

(2010) Yanping Zhu et al.   Nature Chemical Biology

Elevated cleavage of human immunoglobulin gamma molecules containing a lambda light chain mediated by iron and histidine

(2009) David Ouellette et al.   ANALYTICAL BIOCHEMISTRY

Human sialidase inhibitors: Design, synthesis, and biological evaluation of 4-acetamido-5-acylamido-2-fluoro benzoic acids

(2009) Sadagopan Magesh et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Strategies for analysing and improving the expression and quality of recombinant proteins made in mammalian cells

(2009) Nigel Jenkins et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Degradation of an Fc-fusion recombinant protein by host cell proteases: Identification of a CHO cathepsin D protease

(2009) Flavie Robert et al.   BIOTECHNOLOGY AND BIOENGINEERING

A Novel Galactosyltransferase Inhibitor with Diamino Sugar as a Pyrophosphate Mimic

(2009) Nobuyuki Mitsuhashi et al.   EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

Optimal and consistent protein glycosylation in mammalian cell culture

(2009) P. Hossler et al.   GLYCOBIOLOGY

Novel mannosidase inhibitors probe glycoprotein degradation pathways in cells

(2009) Terry D. Butters et al.   GLYCOCONJUGATE JOURNAL

Alpha Mannosidase Inhibitory Effect of Some Iranian Plant Extracts

(2009) A. Gholamhoseinian et al.   International Journal of Pharmacology

Discovery and Investigation of Misincorporation of Serine at Asparagine Positions in Recombinant Proteins Expressed in Chinese Hamster Ovary Cells

(2009) Dingyi Wen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Use of NaCl prevents aggregation of recombinant COMP–Angiopoietin-1 in Chinese hamster ovary cells

(2009) Hye Kyung Ju et al.   JOURNAL OF BIOTECHNOLOGY

Increasing the sialylation of therapeutic glycoproteins: The potential of the sialic acid biosynthetic pathway

(2009) Kaya Bork et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Methionine, tryptophan, and histidine oxidation in a model protein, PTH: Mechanisms and stabilization

(2009) Junyan A. Ji et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Altered metabolism of copper, zinc, and magnesium is associated with increased levels of glycated hemoglobin in patients with diabetes mellitus

(2009) Alena Viktorínová et al.   METABOLISM-CLINICAL AND EXPERIMENTAL

Glycosylation as a strategy to improve antibody-based therapeutics

(2009) Roy Jefferis   NATURE REVIEWS DRUG DISCOVERY

Class I  -Mannosidases Are Required for N-Glycan Processing and Root Development in Arabidopsis thaliana

(2009) E. Liebminger et al.   PLANT CELL

Method to Differentiate Asn Deamidation That Occurred Prior to and during Sample Preparation of a Monoclonal Antibody

(2008) Georgeen Gaza-Bulseco et al.   ANALYTICAL CHEMISTRY

C-terminal lysine variants in fully human monoclonal antibodies: Investigation of test methods and possible causes

(2008) Lawrence W. Dick et al.   BIOTECHNOLOGY AND BIOENGINEERING

The Molecular Basis of Inhibition of Golgi α-Mannosidase II by Mannostatin A

(2008) Douglas A. Kuntz  et al.   CHEMBIOCHEM

Monoclonal Antibody Pharmacokinetics and Pharmacodynamics

(2008) W Wang et al.   CLINICAL PHARMACOLOGY & THERAPEUTICS

Heterogeneity of Monoclonal Antibodies Revealed by Charge-Sensitive Methods

(2008) J. Vlasak et al.   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

Deoxygenated Disaccharide Analogs as Specific Inhibitors of β1–4-Galactosyltransferase 1 and Selectin-mediated Tumor Metastasis

(2008) Jillian R. Brown et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Protein aggregation: Pathways, induction factors and analysis

(2008) Hanns-Christian Mahler et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Post-translational Modifications of Recombinant Proteins: Significance for Biopharmaceuticals

(2008) Nigel Jenkins et al.   MOLECULAR BIOTECHNOLOGY

A study in glycation of a therapeutic recombinant humanized monoclonal antibody: Where it is, how it got there, and how it affects charge-based behavior

(2007) Cynthia Quan et al.   ANALYTICAL BIOCHEMISTRY

Heterogeneity of Monoclonal Antibodies

(2007) Hongcheng Liu et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Enhanced Production of Monomeric Interferon-β by CHO Cells through the Control of Culture Conditions

(2005) J. Rodriguez et al.   BIOTECHNOLOGY PROGRESS

Effect of Copper Sulfate on Performance of a Serum-Free CHO Cell Culture Process and the Level of Free Thiol in the Recombinant Antibody Expressed

(2005) Wendy B. Chaderjian et al.   BIOTECHNOLOGY PROGRESS