Recent advances in technology supporting biopharmaceutical production from mammalian cells

Novel roles for the IgG Fc glycan

(2012) Robert M. Anthony et al.   Annals of the New York Academy of Sciences

Examination of a genetic algorithm for the application in high-throughput downstream process development

(2012) Katrin Treier et al.   Biotechnology Journal

Analysis of dynamic changes in the proteome of a Bcl-XL overexpressing Chinese hamster ovary cell culture during exponential and stationary phases

(2012) Tyler Carlage et al.   BIOTECHNOLOGY PROGRESS

State-of-the-art in downstream processing of monoclonal antibodies: Process trends in design and validation

(2012) P. A. Marichal-Gallardo et al.   BIOTECHNOLOGY PROGRESS

Susceptibility of the PER.C6 cell line for infection with clinical human respiratory syncytial virus isolates

(2012) Lieselot Houspie et al.   JOURNAL OF VIROLOGICAL METHODS

Which are the antibodies to watch in 2012?

(2012) Janice M. Reichert   mAbs

Global metabolic inhibitors of sialyl- and fucosyltransferases remodel the glycome

(2012) Cory D Rillahan et al.   Nature Chemical Biology

Glycan variability on a recombinant IgG antibody transiently produced in HEK-293E cells

(2012) Sophie Nallet et al.   New Biotechnology

Use of the α-mannosidase I inhibitor kifunensine allows the crystallization of apo CTLA-4 homodimer produced in long-term cultures of Chinese hamster ovary cells

(2011) Chao Yu et al.   Acta Crystallographica Section F-Structural Biology and Crystallization Communications

Proteomic understanding of intracellular responses of recombinant Chinese hamster ovary cells cultivated in serum-free medium supplemented with hydrolysates

(2011) Jee Yon Kim et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Evaluation of protein engineering and process optimization approaches to enhance antibody drug manufacturability

(2011) Greg P. Conley et al.   BIOTECHNOLOGY AND BIOENGINEERING

Inhibition of glutamine-dependent autophagy increases t-PA production in CHO Cell fed-batch processes

(2011) Mario A. Jardon et al.   BIOTECHNOLOGY AND BIOENGINEERING

Autophagy and apoptosis of recombinant Chinese hamster ovary cells during fed-batch culture: Effect of nutrient supplementation

(2011) Young Kue Han et al.   BIOTECHNOLOGY AND BIOENGINEERING

Microarray expression profiling identifies genes regulating sustained cell specific productivity (S-Qp) in CHO K1 production cell lines

(2011) Padraig Doolan et al.   Biotechnology Journal

Defining viability in mammalian cell cultures

(2011) Susan M. Browne et al.   BIOTECHNOLOGY LETTERS

Overcoming challenges in WAVE bioreactors without feedback controls for pH and dissolved oxygen

(2011) Inn H. Yuk et al.   BIOTECHNOLOGY PROGRESS

Performance of high intensity fed-batch mammalian cell cultures in disposable bioreactor systems

(2011) John Paul Smelko et al.   BIOTECHNOLOGY PROGRESS

Predicting mab product yields from cultivation media components, using near-infrared and 2D-fluorescence spectroscopies

(2011) Gledson E. Jose et al.   BIOTECHNOLOGY PROGRESS

Necroptosis: An emerging form of programmed cell death

(2011) Wei Wu et al.   CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY

Manufacturing recombinant proteins in kg-ton quantities using animal cells in bioreactors

(2011) Maria De Jesus et al.   EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS

The relevance of tick bites to the production of IgE antibodies to the mammalian oligosaccharide galactose-α-1,3-galactose

(2011) Scott P. Commins et al.   JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

Bcl-xL overexpression delays the onset of autophagy and apoptosis in hyperosmotic recombinant Chinese hamster ovary cell cultures

(2011) Young Kue Han et al.   JOURNAL OF BIOTECHNOLOGY

Large scale microarray profiling and coexpression network analysis of CHO cells identifies transcriptional modules associated with growth and productivity

(2011) Colin Clarke et al.   JOURNAL OF BIOTECHNOLOGY

Necroptosis, necrostatins and tissue injury

(2011) Christopher C.T. Smith et al.   JOURNAL OF CELLULAR AND MOLECULAR MEDICINE

Designing new monoclonal antibody purification processes using mixed-mode chromatography sorbents

(2011) Magali Toueille et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

What's fueling the biotech engine—2010 to 2011

(2011) Saurabh Aggarwal   NATURE BIOTECHNOLOGY

First CHO genome

(2011) Florian M Wurm et al.   NATURE BIOTECHNOLOGY

The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line

(2011) Xun Xu et al.   NATURE BIOTECHNOLOGY

Necroptosis: Biochemical, Physiological and Pathological Aspects

(2011) Zsuzsanna Dunai et al.   PATHOLOGY & ONCOLOGY RESEARCH

Towards Protein Crystallization as a Process Step in Downstream Processing of Therapeutic Antibodies: Screening and Optimization at Microbatch Scale

(2011) Yuguo Zang et al.   PLoS One

Autophagy and apoptosis: what is the connection?

(2011) Jacob M. Gump et al.   TRENDS IN CELL BIOLOGY

MDCK and Vero cells for influenza virus vaccine production: a one-to-one comparison up to lab-scale bioreactor cultivation

(2010) Yvonne Genzel et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Engineering mammalian cells in bioprocessing – current achievements and future perspectives

(2010) Yiping Lim et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Highly efficient deletion of FUT8 in CHO cell lines using zinc-finger nucleases yields cells that produce completely nonfucosylated antibodies

(2010) Laetitia Malphettes et al.   BIOTECHNOLOGY AND BIOENGINEERING

Using partition designs to enhance purification process understanding

(2010) John Pieracci et al.   BIOTECHNOLOGY AND BIOENGINEERING

Profiling of N-glycosylation gene expression in CHO cell fed-batch cultures

(2010) Danny Chee Furng Wong et al.   BIOTECHNOLOGY AND BIOENGINEERING

Cell line-specific control of recombinant monoclonal antibody production by CHO cells

(2010) Peter M. O'Callaghan et al.   BIOTECHNOLOGY AND BIOENGINEERING

A single-use purification process for the production of a monoclonal antibody produced in a PER.C6 human cell line

(2010) Michael Kuczewski et al.   Biotechnology Journal

Experimental characterization of flow conditions in 2- and 20-l bioreactors with wave-induced motion

(2010) Andreas Kalmbach et al.   BIOTECHNOLOGY PROGRESS

Cell death in mammalian cell culture: molecular mechanisms and cell line engineering strategies

(2010) Britta Krampe et al.   CYTOTECHNOLOGY

Discovery and basic pharmacology of erythropoiesis-stimulating agents (ESAs), including the hyperglycosylated ESA, darbepoetin alfa: an update of the rationale and clinical impact

(2010) Zoltán Kiss et al.   EUROPEAN JOURNAL OF CLINICAL PHARMACOLOGY

Using precipitation by polyamines as an alternative to chromatographic separation in antibody purification processes

(2010) Junfen Ma et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Optimized Workflow for Preparation of APTS-Labeled N-Glycans Allowing High-Throughput Analysis of Human Plasma Glycomes using 48-Channel Multiplexed CGE-LIF

(2010) L. Renee Ruhaak et al.   JOURNAL OF PROTEOME RESEARCH

Recovery and purification process development for monoclonal antibody production

(2010) Hui F. Liu et al.   mAbs

Chinese hamster ovary cells can produce galactose-α-1,3-galactose antigens on proteins

(2010) Carlos J Bosques et al.   NATURE BIOTECHNOLOGY

Development of serum-free medium supplemented with hydrolysates for the production of therapeutic antibodies in CHO cell cultures using design of experiments

(2009) Sung Hyun Kim et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Expression of anti-apoptosis genes alters lactate metabolism of Chinese Hamster Ovary cells in culture

(2009) Haimanti Dorai et al.   BIOTECHNOLOGY AND BIOENGINEERING

Differential tumor-targeting abilities of three single-domain antibody formats

(2009) Andrea Bell et al.   CANCER LETTERS

Immunotherapy of Malignant Disease with Tumor Antigen-Specific Monoclonal Antibodies

(2009) M. Campoli et al.   CLINICAL CANCER RESEARCH

Continuous cell lines as a production system for influenza vaccines

(2009) Yvonne Genzel et al.   Expert Review of Vaccines

The distinctive separation attributes of mixed-mode resins and their application in monoclonal antibody downstream purification process

(2009) Jie Chen et al.   JOURNAL OF CHROMATOGRAPHY A

Sensitive and Specific Detection of the Non-Human Sialic Acid N-Glycolylneuraminic Acid In Human Tissues and Biotherapeutic Products

(2009) Sandra L. Diaz et al.   PLoS One

NS0 cell damage by high gas velocity sparging in protein-free and cholesterol-free cultures

(2008) Ying Zhu et al.   BIOTECHNOLOGY AND BIOENGINEERING

Cetuximab-Induced Anaphylaxis and IgE Specific for Galactose-α-1,3-Galactose

(2008) Christine H. Chung et al.   NEW ENGLAND JOURNAL OF MEDICINE

Improved production of recombinant human antithrombin III in Chinese hamster ovary cells by ATF4 overexpression

(2007) Tomoshi Ohya et al.   BIOTECHNOLOGY AND BIOENGINEERING

Nutrient deprivation induces autophagy as well as apoptosis in Chinese hamster ovary cell culture

(2007) Sun Ok Hwang et al.   BIOTECHNOLOGY AND BIOENGINEERING

Serum-free transient protein production system based on adenoviral vector and PER.C6 technology: High yield and preserved bioactivity

(2007) M.J.E. Havenga et al.   BIOTECHNOLOGY AND BIOENGINEERING

Comparison of standard and new generation hydrophobic interaction chromatography resins in the monoclonal antibody purification process

(2007) Jie Chen et al.   JOURNAL OF CHROMATOGRAPHY A