Enzyme engineering: Reshaping the biocatalytic functions

Emerging contaminants of high concern and their enzyme-assisted biodegradation – A review

(2019) Muhammad Bilal et al.   ENVIRONMENT INTERNATIONAL

Modifying bio-catalytic properties of enzymes for efficient biocatalysis: a review from immobilization strategies viewpoint

(2019) Muhammad Bilal et al.   BIOCATALYSIS AND BIOTRANSFORMATION

Tailoring Multipurpose Biocatalysts via Protein Engineering Approaches: A Review

(2019) Muhammad Bilal et al.   CATALYSIS LETTERS

Chemical, physical, and biological coordination: An interplay between materials and enzymes as potential platforms for immobilization

(2019) Muhammad Bilal et al.   COORDINATION CHEMISTRY REVIEWS

Mitigation of bisphenol A using an array of laccase-based robust bio-catalytic cues – A review

(2019) Muhammad Bilal et al.   SCIENCE OF THE TOTAL ENVIRONMENT

Biocatalytic degradation/redefining “removal” fate of pharmaceutically active compounds and antibiotics in the aquatic environment

(2019) Muhammad Bilal et al.   SCIENCE OF THE TOTAL ENVIRONMENT

Persistence of pesticides-based contaminants in the environment and their effective degradation using laccase-assisted biocatalytic systems

(2019) Muhammad Bilal et al.   SCIENCE OF THE TOTAL ENVIRONMENT

Directed Evolution of Protein Catalysts

(2018) Cathleen Zeymer et al.   Annual Review of Biochemistry

Identifying and engineering a critical amino acid residue to enhance the catalytic efficiency of Pseudomonas sp. methyl parathion hydrolase

(2018) Yingnan Li et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Structure-based protein engineering of bacterial β-xylosidase to increase the production yield of xylobiose from xylose

(2018) Seokho Hong et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Protein-engineering of chitosanase from Bacillus sp. MN to alter its substrate specificity

(2018) Eva K. Regel et al.   BIOTECHNOLOGY AND BIOENGINEERING

Protein Crystallography and Site-Direct Mutagenesis Analysis of the Poly(ethylene terephthalate) Hydrolase PETase from Ideonella sakaiensis

(2018) Bing Liu et al.   CHEMBIOCHEM

Immobilization of peroxidase on polypyrrole-cellulose-graphene oxide nanocomposite via non-covalent interactions for the degradation of Reactive Blue 4 dye

(2018) Misha Ali et al.   CHEMOSPHERE

Speeding up enzyme discovery and engineering with ultrahigh-throughput methods

(2018) Hans Adrian Bunzel et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Guar gum blended alginate/agarose hydrogel as a promising support for the entrapment of peroxidase: Stability and reusability studies for the treatment of textile effluent

(2018) Misha Ali et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Significant improvement of the nitrilase activity by semi-rational protein engineering and its application in the production of iminodiacetic acid

(2018) Zhi-Qiang Liu et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

“Smart” chemistry and its application in peroxidase immobilization using different support materials

(2018) Muhammad Bilal et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Characterization and engineering of a plastic-degrading aromatic polyesterase

(2018) Harry P. Austin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Nano-peroxidase fabrication on cation exchanger nanocomposite: Augmenting catalytic efficiency and stability for the decolorization and detoxification of Methyl Violet 6B dye

(2018) Misha Ali et al.   SEPARATION AND PURIFICATION TECHNOLOGY

Characterization and protein engineering of a novel versatile fungal polyphenol oxidase with chlorophenol bioremediation potential

(2018) Efstratios Nikolaivits et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

The state-of-the-art strategies of protein engineering for enzyme stabilization

(2018) Qian Liu et al.   BIOTECHNOLOGY ADVANCES

One-step combined focused epPCR and saturation mutagenesis for thermostability evolution of a new cold-active xylanase

(2017) Juan Pablo Acevedo et al.   ENZYME AND MICROBIAL TECHNOLOGY

Repurposing designed mutants: a valuable strategy for computer-aided laccase engineering – the case of POXA1b

(2017) Valerio Guido Giacobelli et al.   Catalysis Science & Technology

Rational engineering of a native hyperthermostable lactonase into a broad spectrum phosphotriesterase

(2017) Pauline Jacquet et al.   Scientific Reports

Engineering a short-chain dehydrogenase/reductase for the stereoselective production of (2S,3R,4S)-4-hydroxyisoleucine with three asymmetric centers

(2017) Xuan Shi et al.   Scientific Reports

Solubilization and Iterative Saturation Mutagenesis of α1,3-fucosyltransferase fromHelicobacter pylorito enhance its catalytic efficiency

(2016) Yun Hee Choi et al.   BIOTECHNOLOGY AND BIOENGINEERING

Improvement of alkalophilicity of an alkaline xylanase Xyn11A-LC from Bacillus sp. SN5 by random mutation and Glu135 saturation mutagenesis

(2016) Wenqin Bai et al.   BMC BIOTECHNOLOGY

The Generation and Exploitation of Protein Mutability Landscapes for Enzyme Engineering

(2016) Jan-Ytzen van der Meer et al.   CHEMBIOCHEM

Ultrahigh-throughput–directed enzyme evolution by absorbance-activated droplet sorting (AADS)

(2016) Fabrice Gielen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Enhanced Catalytic Activity and Stability of Ginger Peroxidase Immobilized on Amino-Functionalized Silica-Coated Titanium Dioxide Nanocomposite: A Cost-Effective Tool for Bioremediation

(2016) Misha Ali et al.   WATER AIR AND SOIL POLLUTION

Computer-Aided Laccase Engineering: Toward Biological Oxidation of Arylamines

(2016) Gerard Santiago et al.   ACS Catalysis

Engineering of Candida glabrata Ketoreductase 1 for Asymmetric Reduction of α-Halo Ketones

(2016) Fengyu Qin et al.   ACS Catalysis

Bacillus pumilus Cyanide Dihydratase Mutants with Higher Catalytic Activity

(2016) Mary A. Crum et al.   Frontiers in Microbiology

Engineering of TM1459 from Thermotoga maritima for Increased Oxidative Alkene Cleavage Activity

(2016) Matthias Fink et al.   Frontiers in Microbiology

Improvements in Glucose Sensitivity and Stability of Trichoderma reesei β-Glucosidase Using Site-Directed Mutagenesis

(2016) Boyang Guo et al.   PLoS One

Creating Space for Large Acceptors: Rational Biocatalyst Design for Resveratrol Glycosylation in an Aqueous System

(2015) Mareike E. Dirks-Hofmeister et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Improving the catalytic performance of a GH11 xylanase by rational protein engineering

(2015) Ya-Shan Cheng et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Engineering the Active Site of the Amine Transaminase fromVibrio fluvialisfor the Asymmetric Synthesis of Aryl-Alkyl Amines and Amino Alcohols

(2015) Alberto Nobili et al.   ChemCatChem

De novo protein design: how do we expand into the universe of possible protein structures?

(2015) Derek N Woolfson et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

The Industrial Age of Biocatalytic Transamination

(2015) Michael Fuchs et al.   EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

Significantly improved thermostability of a reductase CgKR1 from Candida glabrata with a key mutation at Asp 138 for enhancing bioreduction of aromatic α-keto esters

(2015) Lei Huang et al.   JOURNAL OF BIOTECHNOLOGY

Insights into Laccase Engineering from Molecular Simulations: Toward a Binding-Focused Strategy

(2015) Emanuele Monza et al.   Journal of Physical Chemistry Letters

Engineering of Kuma030: A Gliadin Peptidase That Rapidly Degrades Immunogenic Gliadin Peptides in Gastric Conditions

(2015) Clancey Wolf et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Engineering of Helicobacter pylori L-Asparaginase: Characterization of Two Functionally Distinct Groups of Mutants

(2015) Maristella Maggi et al.   PLoS One

Rational design of organophosphorus hydrolase with high catalytic efficiency for detoxifying a V-type nerve agent

(2014) Young-Su Jeong et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Protein engineering of laccase to enhance its activity and stability in the presence of organic solvents

(2014) Behnam Rasekh et al.   ENGINEERING IN LIFE SCIENCES

High Throughput Screening and Selection Methods for Directed Enzyme Evolution

(2014) Han Xiao et al.   INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH

Evolution of enzyme catalysts caged in biomimetic gel-shell beads

(2014) Martin Fischlechner et al.   Nature Chemistry

SWISS-MODEL: modelling protein tertiary and quaternary structure using evolutionary information

(2014) Marco Biasini et al.   NUCLEIC ACIDS RESEARCH

Engineering Neprilysin Activity and Specificity to Create a Novel Therapeutic for Alzheimer’s Disease

(2014) Carl I. Webster et al.   PLoS One

A system for the continuous directed evolution of proteases rapidly reveals drug-resistance mutations

(2014) Bryan C. Dickinson et al.   Nature Communications

Computational Enzyme Design

(2013) Gert Kiss et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Protein Engineering by Random Mutagenesis and Structure-Guided Consensus of Geobacillus stearothermophilus Lipase T6 for Enhanced Stability in Methanol

(2013) Adi Dror et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Engineered thermostable fungal Cel6A and Cel7A cellobiohydrolases hydrolyze cellulose efficiently at elevated temperatures

(2013) Indira Wu et al.   BIOTECHNOLOGY AND BIOENGINEERING

Lipase-catalyzed process for biodiesel production: Protein engineering and lipase production

(2013) Hyun Tae Hwang et al.   BIOTECHNOLOGY AND BIOENGINEERING

Engineering of a bacterial tyrosinase for improved catalytic efficiency towards D-tyrosine using random and site directed mutagenesis approaches

(2013) Susan Molloy et al.   BIOTECHNOLOGY AND BIOENGINEERING

Enzyme immobilisation in biocatalysis: why, what and how

(2013) Roger A. Sheldon et al.   CHEMICAL SOCIETY REVIEWS

De novo enzymes by computational design

(2013) Hajo Kries et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Protein engineering of enzymes for process applications

(2013) John M Woodley   CURRENT OPINION IN CHEMICAL BIOLOGY

Use of ‘small but smart’ libraries to enhance the enantioselectivity of an esterase fromBacillus stearothermophilustowards tetrahydrofuran-3-yl acetate

(2013) Alberto Nobili et al.   FEBS Journal

Molecular Engineering of Organophosphate Hydrolysis Activity from a Weak Promiscuous Lactonase Template

(2013) Monika M. Meier et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Engineering an Enantioselective Amine Oxidase for the Synthesis of Pharmaceutical Building Blocks and Alkaloid Natural Products

(2013) Diego Ghislieri et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Engineering the loops in a lipase for stability in DMSO

(2013) Poornima Yedavalli et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Improved Thermostability of Clostridium thermocellum Endoglucanase Cel8A by Using Consensus-Guided Mutagenesis

(2012) Michael Anbar et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Biological and enzymatic treatment of bisphenol A and other endocrine disrupting compounds: a review

(2012) Qayyum Husain et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Computational Design of an α-Gliadin Peptidase

(2012) Sydney R. Gordon et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Redesign of enzyme for improving catalytic activity and enantioselectivity toward poor substrates: manipulation of the transition state

(2012) Tadashi Ema et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Enhanced activity of Thermotoga maritima cellulase 12A by mutating a unique surface loop

(2011) Ya-Shan Cheng et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Immobilized Peroxidase as a Valuable Tool in the Remediation of Aromatic Pollutants and Xenobiotic Compounds: A Review

(2011) Qayyum Husain et al.   CRITICAL REVIEWS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGY

Hyperthermophilic asparaginase mutants with enhanced substrate affinity and antineoplastic activity: structural insights on their mechanism of action

(2011) Saurabh Bansal et al.   FASEB JOURNAL

Engineering substrate specificity of E. carotovora l-asparaginase for the development of biosensor

(2011) Georgia A. Kotzia et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

A system for the continuous directed evolution of biomolecules

(2011) Kevin M. Esvelt et al.   NATURE

Directed evolution of hydrolases for prevention of G-type nerve agent intoxication

(2011) Rinkoo D Gupta et al.   Nature Chemical Biology

De Novo Enzyme Design Using Rosetta3

(2011) Florian Richter et al.   PLoS One

A general strategy for the evolution of bond-forming enzymes using yeast display

(2011) I. Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Thermostability Enhancement of Clostridium thermocellum Cellulosomal Endoglucanase Cel8A by a Single Glycine Substitution

(2010) Michael Anbar et al.   ChemCatChem

In search of a catalytic bioscavenger for the prophylaxis of nerve agent toxicity

(2010) Robert C. diTargiani et al.   CHEMICO-BIOLOGICAL INTERACTIONS

Laboratory Evolution of High-Redox Potential Laccases

(2010) Diana Maté et al.   CHEMISTRY & BIOLOGY

Beyond directed evolution—semi-rational protein engineering and design

(2010) Stefan Lutz   CURRENT OPINION IN BIOTECHNOLOGY

Enhanced thermostability of methyl parathion hydrolase from Ochrobactrum sp. M231 by rational engineering of a glycine to proline mutation

(2010) Jian Tian et al.   FEBS Journal

Improving thermostability of papain through structure-based protein engineering

(2010) Debi Choudhury et al.   PROTEIN ENGINEERING DESIGN & SELECTION

3DM: Systematic analysis of heterogeneous superfamily data to discover protein functionalities

(2010) Remko K. Kuipers et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Ultrahigh-Throughput FACS-Based Screening for Directed Enzyme Evolution

(2009) Guangyu Yang et al.   CHEMBIOCHEM

Computational tools for designing and engineering biocatalysts

(2009) Jiri Damborsky et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Engineering thermal stability of l-asparaginase by in vitro directed evolution

(2009) Georgia A. Kotzia et al.   FEBS Journal

Directed evolution of enzymes: Library screening strategies

(2009) Hans Leemhuis et al.   IUBMB LIFE

The effect of substitution of Phe181 and Phe182 with Ala on activity, substrate specificity and stabilization of substrate at the active site of Bacillus thermocatenulatus lipase

(2009) Ali Asghar Karkhane et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity

(2009) Jean-Christophe Baret et al.   LAB ON A CHIP

Redesigning dehalogenase access tunnels as a strategy for degrading an anthropogenic substrate

(2009) Martina Pavlova et al.   Nature Chemical Biology

Construction and flow cytometric screening of targeted enzyme libraries

(2009) Navin Varadarajan et al.   Nature Protocols

HotSpot Wizard: a web server for identification of hot spots in protein engineering

(2009) A. Pavelka et al.   NUCLEIC ACIDS RESEARCH

Rational stabilization of enzymes by computational redesign of surface charge–charge interactions

(2009) Alexey V. Gribenko et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Peroxidase mediated decolorization and remediation of wastewater containing industrial dyes: a review

(2009) Qayyum Husain   REVIEWS IN ENVIRONMENTAL SCIENCE AND BIO-TECHNOLOGY

Engineered enzymes for chemical production

(2008) Stephan Luetz et al.   BIOTECHNOLOGY AND BIOENGINEERING

Multiple protein sequence alignment

(2008) Jimin Pei   CURRENT OPINION IN STRUCTURAL BIOLOGY

Thermostable Bacillus subtilis Lipases: In Vitro Evolution and Structural Insight

(2008) Shoeb Ahmad et al.   JOURNAL OF MOLECULAR BIOLOGY

Protein engineering of improved prolyl endopeptidases for celiac sprue therapy

(2008) Jennifer Ehren et al.   PROTEIN ENGINEERING DESIGN & SELECTION

New opportunities for biocatalysis: making pharmaceutical processes greener

(2008) John M. Woodley   TRENDS IN BIOTECHNOLOGY