Protein Engineering by Random Mutagenesis and Structure-Guided Consensus of Geobacillus stearothermophilus Lipase T6 for Enhanced Stability in Methanol

Unraveling the Rationale Behind Organic Solvent Stability of Lipases

(2012) Debamitra Chakravorty et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Stabilization of Candida antarctica lipase B in hydrophilic organic solvent by rational design of hydrogen bond

(2012) Hyun June Park et al.   BIOTECHNOLOGY AND BIOPROCESS ENGINEERING

An organic solvent and thermally stable lipase from Burkholderia ambifaria YCJ01: Purification, characteristics and application for chiral resolution of mandelic acid

(2012) Changjin Yao et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Application of a Burkholderia cepacia lipase-immobilized silica monolith to batch and continuous biodiesel production with a stoichiometric mixture of methanol and crude Jatropha oil

(2011) Koei Kawakami et al.   Biotechnology for Biofuels

Biodiesel production using enzymatic transesterification – Current state and perspectives

(2011) Adriana Gog et al.   RENEWABLE ENERGY

Isolation and Characterization of a Novel Thermophilic-Organic Solvent Stable Lipase From Acinetobacter baylyi

(2010) Sasithorn Uttatree et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Increasing the stability of an enzyme toward hostile organic solvents by directed evolution based on iterative saturation mutagenesis using the B-FIT method

(2010) Manfred T. Reetz et al.   CHEMICAL COMMUNICATIONS

Status of protein engineering for biocatalysts: how to design an industrially useful biocatalyst

(2010) Andreas S Bommarius et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Directed evolution of tyrosinase for enhanced monophenolase/diphenolase activity ratio

(2010) Vered Shuster Ben-Yosef et al.   ENZYME AND MICROBIAL TECHNOLOGY

Enhanced activity and stability in the presence of organic solvents by increased active site polarity and stabilization of a surface loop in a metalloprotease

(2010) A. Badoei-Dalfard et al.   JOURNAL OF BIOCHEMISTRY

Catalytic properties and potential applications of Bacillus lipases

(2010) Maya Guncheva et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

ConSurf 2010: calculating evolutionary conservation in sequence and structure of proteins and nucleic acids

(2010) H. Ashkenazy et al.   NUCLEIC ACIDS RESEARCH

Creation of an Amino Acid Network of Structurally Coupled Residues in the Directed Evolution of a Thermostable Enzyme

(2009) Manfred T. Reetz et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Organic solvent-tolerant enzymes

(2009) Noriyuki Doukyu et al.   BIOCHEMICAL ENGINEERING JOURNAL

Directed Evolution of Serum Paraoxonase PON3 by Family Shuffling and Ancestor/Consensus Mutagenesis, and Its Biochemical Characterization

(2009) Olga Khersonsky et al.   BIOCHEMISTRY

Enhancement of the organic solvent-stability of the LST-03 lipase by directed evolution

(2009) Takuya Kawata et al.   BIOTECHNOLOGY PROGRESS

Biodiesel fuel production via transesterification of oils using lipase biocatalyst

(2009) MAN XIAO et al.   Global Change Biology Bioenergy

Biodiesel production through lipase catalyzed transesterification: An overview

(2009) Akhil Bajaj et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Finding better protein engineering strategies

(2009) Romas J Kazlauskas et al.   Nature Chemical Biology

Directed evolution drives the next generation of biocatalysts

(2009) Nicholas J Turner   Nature Chemical Biology

Perspectives for biotechnological production of biodiesel and impacts

(2008) Wei Du et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Addressing the Numbers Problem in Directed Evolution

(2008) Manfred T. Reetz et al.   CHEMBIOCHEM

Enhancement of Novozym-435 catalytic properties by physical or chemical modification

(2008) Zaida Cabrera et al.   PROCESS BIOCHEMISTRY

Thermostable variants constructed via the structure-guided consensus method also show increased stability in salts solutions and homogeneous aqueous-organic media

(2008) E. Vazquez-Figueroa et al.   PROTEIN ENGINEERING DESIGN & SELECTION