Recent Advances of CRISPR/Cas9-Based Genetic Engineering and Transcriptional Regulation in Industrial Biology

Fast gene disruption in Trichoderma reesei using in vitro assembled Cas9/gRNA complex

(2019) Zhenzhen Hao et al.   BMC BIOTECHNOLOGY

Application of the CRISPR/Cas system for genome editing in microalgae

(2019) Yu-Ting Zhang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

CRISPR-mediated genome editing in non-conventional yeasts for biotechnological applications

(2019) Peng Cai et al.   Microbial Cell Factories

CRISPR system in the yeast Saccharomyces cerevisiae and its application in the bioproduction of useful chemicals

(2019) Ryosuke Mitsui et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts: Current state and future prospects

(2018) Hana Raschmanová et al.   BIOTECHNOLOGY ADVANCES

Genetic tool development and systemic regulation in biosynthetic technology

(2018) Zhongxue Dai et al.   Biotechnology for Biofuels

CRISPR/Cas9 Assisted Multiplex Genome Editing Technique in Escherichia coli

(2018) Xu Feng et al.   Biotechnology Journal

Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System

(2018) Jiazhang Lian et al.   Biotechnology Journal

Hijacking CRISPR-Cas for high-throughput bacterial metabolic engineering: advances and prospects

(2018) Ioannis Mougiakos et al.   CURRENT OPINION IN BIOTECHNOLOGY

A CRISPR–dCas Toolbox for Genetic Engineering and Synthetic Biology

(2018) Xiaoshu Xu et al.   JOURNAL OF MOLECULAR BIOLOGY

Advances in Industrial Biotechnology Using CRISPR-Cas Systems

(2018) Paul D. Donohoue et al.   TRENDS IN BIOTECHNOLOGY

CRISPR/Cas9 Assisted Multiplex Genome Editing Technique in Escherichia coli

(2018) Xu Feng et al.   Biotechnology Journal

Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System

(2018) Jiazhang Lian et al.   Biotechnology Journal

Efficient CRISPR–Cas9 mediated multiplex genome editing in yeasts

(2018) Laiyou Wang et al.   Biotechnology for Biofuels

Heterologous Production of Squalene from Glucose in Engineered Corynebacterium glutamicum Using Multiplex CRISPR Interference and High-Throughput Fermentation

(2018) Jaehyun Park et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Efficient genome editing by CRISPR/Cas9 with a tRNA-sgRNA fusion in the methylotrophic yeast Ogataea polymorpha

(2017) Minori Numamoto et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Expanding the CRISPR/Cas9 toolkit for Pichia pastoris with efficient donor integration and alternative resistance markers

(2017) Astrid Weninger et al.   JOURNAL OF CELLULAR BIOCHEMISTRY

A comparison of Agrobacterium -mediated transformation and protoplast-mediated transformation with CRISPR-Cas9 and bipartite gene targeting substrates, as effective gene targeting tools for Aspergillus carbonarius

(2017) István Weyda et al.   JOURNAL OF MICROBIOLOGICAL METHODS

A two-plasmid inducible CRISPR/Cas9 genome editing tool for Clostridium acetobutylicum

(2017) François Wasels et al.   JOURNAL OF MICROBIOLOGICAL METHODS

CRISPR/Cas9-coupled recombineering for metabolic engineering of Corynebacterium glutamicum

(2017) Jae Sung Cho et al.   METABOLIC ENGINEERING

CRISPR EnAbled Trackable genome Engineering for isopropanol production in Escherichia coli

(2017) Liya Liang et al.   METABOLIC ENGINEERING

Development of a CRISPR/Cas9 genome editing toolbox for Corynebacterium glutamicum

(2017) Jiao Liu et al.   Microbial Cell Factories

In Silico Meets In Vivo : Towards Computational CRISPR-Based sgRNA Design

(2017) Guo-hui Chuai et al.   TRENDS IN BIOTECHNOLOGY

A Highly Efficient CRISPR-Cas9-Mediated Large Genomic Deletion in Bacillus subtilis

(2017) Younju So et al.   Frontiers in Microbiology

Development of a CRISPR-Cas9 Tool Kit for Comprehensive Engineering of Bacillus subtilis

(2016) Adam W. Westbrook et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Extending CRISPR-Cas9 Technology from Genome Editing to Transcriptional Engineering in the Genus Clostridium

(2016) Mark R. Bruder et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Genome editing of Clostridium autoethanogenum using CRISPR/Cas9

(2016) Shilpa Nagaraju et al.   Biotechnology for Biofuels

Highly efficient CRISPR mutagenesis by microhomology-mediated end joining in Aspergillus fumigatus

(2016) Chi Zhang et al.   FUNGAL GENETICS AND BIOLOGY

Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris

(2016) Astrid Weninger et al.   JOURNAL OF BIOTECHNOLOGY

CRISPR-Cas-Assisted Multiplexing (CAM): Simple Same-Day Multi-Locus Engineering in Yeast

(2016) Jessica M. Walter et al.   JOURNAL OF CELLULAR PHYSIOLOGY

Multiplex gene editing of the Yarrowia lipolytica genome using the CRISPR-Cas9 system

(2016) Shuliang Gao et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

CRISPR/Cas9 advances engineering of microbial cell factories

(2016) Tadas Jakočiūnas et al.   METABOLIC ENGINEERING

CRISPR-Cas9 for the genome engineering of cyanobacteria and succinate production

(2016) Hung Li et al.   METABOLIC ENGINEERING

Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering

(2016) Andrew D Garst et al.   NATURE BIOTECHNOLOGY

Functional Reconstitution of a Fungal Natural Product Gene Cluster by Advanced Genome Editing

(2016) Jakob Weber et al.   ACS Synthetic Biology

Synthetic RNA Polymerase III Promoters Facilitate High-Efficiency CRISPR–Cas9-Mediated Genome Editing in Yarrowia lipolytica

(2016) Cory M. Schwartz et al.   ACS Synthetic Biology

Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium

(2016) Michael E. Pyne et al.   Scientific Reports

Efficient Genome Editing in Clostridium cellulolyticum via CRISPR-Cas9 Nickase

(2015) Tao Xu et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Rapid and marker-free refactoring of xylose-fermenting yeast strains with Cas9/CRISPR

(2015) Ching-Sung Tsai et al.   BIOTECHNOLOGY AND BIOENGINEERING

Cellulase production by recombinant Trichoderma reesei and its application in enzymatic hydrolysis of agricultural residues

(2015) Hao Fang et al.   FUEL

Metabolic engineering of Escherichia coli using CRISPR–Cas9 meditated genome editing

(2015) Yifan Li et al.   METABOLIC ENGINEERING

Multiplex metabolic pathway engineering using CRISPR/Cas9 in Saccharomyces cerevisiae

(2015) Tadas Jakočiūnas et al.   METABOLIC ENGINEERING

Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

(2015) Silvana Konermann et al.   NATURE

CasEMBLR: Cas9-Facilitated Multiloci Genomic Integration of in Vivo Assembled DNA Parts in Saccharomyces cerevisiae

(2015) Tadas Jakočiu̅nas et al.   ACS Synthetic Biology

Efficient Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas

(2015) Andrew A. Horwitz et al.   Cell Systems

Efficient genome editing in filamentous fungus Trichoderma reesei using the CRISPR/Cas9 system

(2015) Rui Liu et al.   Cell Discovery

A CRISPR-Cas9 System for Genetic Engineering of Filamentous Fungi

(2015) Christina S. Nødvig et al.   PLoS One

Construction of a Quadruple Auxotrophic Mutant of an Industrial Polyploid Saccharomyces cerevisiae Strain by Using RNA-Guided Cas9 Nuclease

(2014) Guo-Chang Zhang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Accumulation of yellow Monascus pigments by extractive fermentation in nonionic surfactant micelle aqueous solution

(2014) Xu Xiong et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Technical guide for genetic advancement of underdeveloped and intractable Clostridium

(2014) Michael E. Pyne et al.   BIOTECHNOLOGY ADVANCES

Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation

(2014) Luke A. Gilbert et al.   CELL

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells

(2014) Lior Nissim et al.   MOLECULAR CELL

Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks

(2014) A. A. Nielsen et al.   Molecular Systems Biology

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9

(2014) Samuel H. Sternberg et al.   NATURE

CRISPR–Cas9-assisted recombineering in Lactobacillus reuteri

(2014) Jee-Hwan Oh et al.   NUCLEIC ACIDS RESEARCH

Implementation of the CRISPR-Cas9 system in fission yeast

(2014) Jake Z. Jacobs et al.   Nature Communications

Homology-Integrated CRISPR–Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae

(2014) Zehua Bao et al.   ACS Synthetic Biology

Selection of chromosomal DNA libraries using a multiplex CRISPR system

(2014) Owen W Ryan et al.   eLife

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

Fungal arachidonic acid-rich oil: research, development and industrialization

(2013) Xiao-Jun Ji et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

New opportunities by synthetic biology for biopharmaceutical production in Pichia pastoris

(2013) Thomas Vogl et al.   CURRENT OPINION IN BIOTECHNOLOGY

Filamentous fungi are large-scale producers of pigments and colorants for the food industry

(2013) Laurent Dufossé et al.   CURRENT OPINION IN BIOTECHNOLOGY

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system

(2013) David Bikard et al.   NUCLEIC ACIDS RESEARCH

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

(2013) James E. DiCarlo et al.   NUCLEIC ACIDS RESEARCH

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

Optimization of a Yeast RNA Interference System for Controlling Gene Expression and Enabling Rapid Metabolic Engineering

(2013) Nathan C. Crook et al.   ACS Synthetic Biology

Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas

(2013) Fahim Farzadfard et al.   ACS Synthetic Biology

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

CRISPR/Cas, the Immune System of Bacteria and Archaea

(2010) P. Horvath et al.   SCIENCE

Engineering solventogenic clostridia

(2008) Eleftherios T Papoutsakis   CURRENT OPINION IN BIOTECHNOLOGY