Rapid and marker-free refactoring of xylose-fermenting yeast strains with Cas9/CRISPR

Deletion ofPHO13, Encoding Haloacid Dehalogenase Type IIA Phosphatase, Results in Upregulation of the Pentose Phosphate Pathway in Saccharomyces cerevisiae

(2014) Soo Rin Kim et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Construction of a Quadruple Auxotrophic Mutant of an Industrial Polyploid Saccharomyces cerevisiae Strain by Using RNA-Guided Cas9 Nuclease

(2014) Guo-Chang Zhang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

2,3-Butanediol production from cellobiose by engineered Saccharomyces cerevisiae

(2014) Hong Nan et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Advances in metabolic engineering of yeastSaccharomyces cerevisiaefor production of chemicals

(2014) Irina Borodina et al.   Biotechnology Journal

Production of 2,3-butanediol from xylose by engineered Saccharomyces cerevisiae

(2014) Soo-Jung Kim et al.   JOURNAL OF BIOTECHNOLOGY

Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system

(2014) Tetsushi Sakuma et al.   Scientific Reports

Antibacterial gene transfer across the tree of life

(2014) Jason A Metcalf et al.   eLife

Strain engineering of Saccharomyces cerevisiae for enhanced xylose metabolism

(2013) Soo Rin Kim et al.   BIOTECHNOLOGY ADVANCES

A versatile, efficient strategy for assembly of multi-fragment expression vectors in Saccharomyces cerevisiae using 60 bp synthetic recombination sequences

(2013) Niels GA Kuijpers et al.   Microbial Cell Factories

Genome-scale engineering for systems and synthetic biology

(2013) K. M. Esvelt et al.   Molecular Systems Biology

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

(2013) James E. DiCarlo et al.   NUCLEIC ACIDS RESEARCH

Rational and Evolutionary Engineering Approaches Uncover a Small Set of Genetic Changes Efficient for Rapid Xylose Fermentation in Saccharomyces cerevisiae

(2013) Soo Rin Kim et al.   PLoS One

High expression of XYL2 coding for xylitol dehydrogenase is necessary for efficient xylose fermentation by engineered Saccharomyces cerevisiae

(2012) Soo Rin Kim et al.   METABOLIC ENGINEERING

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Simultaneous co-fermentation of mixed sugars: a promising strategy for producing cellulosic ethanol

(2012) Soo Rin Kim et al.   TRENDS IN BIOTECHNOLOGY

FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method

(2011) Chaokun Li et al.   BMC BIOTECHNOLOGY

Effects of NADH-preferring xylose reductase expression on ethanol production from xylose in xylose-metabolizing recombinant Saccharomyces cerevisiae

(2011) Sung-Haeng Lee et al.   JOURNAL OF BIOTECHNOLOGY

Functional Expression of a Bacterial Xylose Isomerase in Saccharomyces cerevisiae

(2009) D. Brat et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Deleting the para-nitrophenyl phosphatase (pNPPase), PHO13, in recombinant Saccharomyces cerevisiae improves growth and ethanol production on d-xylose

(2008) Jennifer Headman Van Vleet et al.   METABOLIC ENGINEERING