Advancing Metabolic Engineering of Saccharomyces cerevisiae Using the CRISPR/Cas System

Systematic testing of enzyme perturbation sensitivities via graded dCas9 modulation in Saccharomyces cerevisiae

(2017) Matthew Deaner et al.   METABOLIC ENGINEERING

Engineering biological systems using automated biofoundries

(2017) Ran Chao et al.   METABOLIC ENGINEERING

SWITCH: a dynamic CRISPR tool for genome engineering and metabolic pathway control for cell factory construction in Saccharomyces cerevisiae

(2017) Katherina García Vanegas et al.   Microbial Cell Factories

Transcriptional reprogramming in yeast using dCas9 and combinatorial gRNA strategies

(2017) Emil D. Jensen et al.   Microbial Cell Factories

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

(2017) Nicole M. Gaudelli et al.   NATURE

Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion

(2017) Yuan Zong et al.   NATURE BIOTECHNOLOGY

CRISPR/Cpf1 enables fast and simple genome editing of Saccharomyces cerevisiae

(2017) René Verwaal et al.   YEAST

Digital logic circuits in yeast with CRISPR-dCas9 NOR gates

(2017) Miles W. Gander et al.   Nature Communications

Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system

(2017) Jiazhang Lian et al.   Nature Communications

Automated multiplex genome-scale engineering in yeast

(2017) Tong Si et al.   Nature Communications

Enabling Graded and Large-Scale Multiplex of Desired Genes Using a Dual-Mode dCas9 Activator in Saccharomyces cerevisiae

(2017) Matthew Deaner et al.   ACS Synthetic Biology

sgRNA Scorer 2.0: A Species-Independent Model To Predict CRISPR/Cas9 Activity

(2017) Raj Chari et al.   ACS Synthetic Biology

Targeting Specificity of the CRISPR/Cas9 System

(2017) Ipek Tasan et al.   ACS Synthetic Biology

Construction of plasmids with tunable copy numbers in Saccharomyces cerevisiae and their applications in pathway optimization and multiplex genome integration

(2016) Jiazhang Lian et al.   BIOTECHNOLOGY AND BIOENGINEERING

EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes intoSaccharomyces cerevisiaevia CRISPR-Cas9

(2016) Mathew M Jessop-Fabre et al.   Biotechnology Journal

Engineering Cellular Metabolism

(2016) Jens Nielsen et al.   CELL

CRISPR/Cas9 advances engineering of microbial cell factories

(2016) Tadas Jakočiūnas et al.   METABOLIC ENGINEERING

A highly efficient single-step, markerless strategy for multi-copy chromosomal integration of large biochemical pathways in Saccharomyces cerevisiae

(2016) Shuobo Shi et al.   METABOLIC ENGINEERING

PHO13 deletion-induced transcriptional activation prevents sedoheptulose accumulation during xylose metabolism in engineered Saccharomyces cerevisiae

(2016) Haiqing Xu et al.   METABOLIC ENGINEERING

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering

(2016) Andrew D Garst et al.   NATURE BIOTECHNOLOGY

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

A Cas9-based toolkit to program gene expression inSaccharomyces cerevisiae

(2016) Amanda Reider Apel et al.   NUCLEIC ACIDS RESEARCH

CHOPCHOP v2: a web tool for the next generation of CRISPR genome engineering

(2016) Kornel Labun et al.   NUCLEIC ACIDS RESEARCH

Teleseismic S wave microseisms

(2016) K. Nishida et al.   SCIENCE

EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes intoSaccharomyces cerevisiaevia CRISPR-Cas9

(2016) Mathew M Jessop-Fabre et al.   Biotechnology Journal

Rapid and marker-free refactoring of xylose-fermenting yeast strains with Cas9/CRISPR

(2015) Ching-Sung Tsai et al.   BIOTECHNOLOGY AND BIOENGINEERING

Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds

(2015) Jesse G. Zalatan et al.   CELL

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae

(2015) Robert Mans et al.   FEMS YEAST RESEARCH

Sequence determinants of improved CRISPR sgRNA design

(2015) Han Xu et al.   GENOME RESEARCH

Multiplex metabolic pathway engineering using CRISPR/Cas9 in Saccharomyces cerevisiae

(2015) Tadas Jakočiūnas et al.   METABOLIC ENGINEERING

The New State of the Art: Cas9 for Gene Activation and Repression

(2015) Marie F. La Russa et al.   MOLECULAR AND CELLULAR BIOLOGY

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

(2015) Benjamin P. Kleinstiver et al.   NATURE

Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library approach

(2015) Raj Chari et al.   NATURE METHODS

Highly efficient Cas9-mediated transcriptional programming

(2015) Alejandro Chavez et al.   NATURE METHODS

High-throughput functional genomics using CRISPR–Cas9

(2015) Ophir Shalem et al.   NATURE REVIEWS GENETICS

Cell–Cell Communication in Yeast Using Auxin Biosynthesis and Auxin Responsive CRISPR Transcription Factors

(2015) Arjun Khakhar et al.   ACS Synthetic Biology

CasEMBLR: Cas9-Facilitated Multiloci Genomic Integration of in Vivo Assembled DNA Parts in Saccharomyces cerevisiae

(2015) Tadas Jakočiu̅nas et al.   ACS Synthetic Biology

Efficient Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas

(2015) Andrew A. Horwitz et al.   Cell Systems

Construction of a Quadruple Auxotrophic Mutant of an Industrial Polyploid Saccharomyces cerevisiae Strain by Using RNA-Guided Cas9 Nuclease

(2014) Guo-Chang Zhang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites

(2014) Yuki Naito et al.   BIOINFORMATICS

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

Recent advances in biosynthesis of fatty acids derived products in Saccharomyces cerevisiae via enhanced supply of precursor metabolites

(2014) Jiazhang Lian et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Design and construction of acetyl-CoA overproducing Saccharomyces cerevisiae strains

(2014) Jiazhang Lian et al.   METABOLIC ENGINEERING

Metabolic engineering of a Saccharomyces cerevisiae strain capable of simultaneously utilizing glucose and galactose to produce enantiopure (2R,3R)-butanediol

(2014) Jiazhang Lian et al.   METABOLIC ENGINEERING

Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation

(2014) John G Doench et al.   NATURE BIOTECHNOLOGY

CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing

(2014) Tessa G. Montague et al.   NUCLEIC ACIDS RESEARCH

Altered sterol composition renders yeast thermotolerant

(2014) L. Caspeta et al.   SCIENCE

Homology-Integrated CRISPR–Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae

(2014) Zehua Bao et al.   ACS Synthetic Biology

Selection of chromosomal DNA libraries using a multiplex CRISPR system

(2014) Owen W Ryan et al.   eLife

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

Self-processing of ribozyme-flanked RNAs into guide RNAsin vitroandin vivofor CRISPR-mediated genome editing

(2013) Yangbin Gao et al.   Journal of Integrative Plant Biology

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

CRISPR RNA–guided activation of endogenous human genes

(2013) Morgan L Maeder et al.   NATURE METHODS

Orthogonal Cas9 proteins for RNA-guided gene regulation and editing

(2013) Kevin M Esvelt et al.   NATURE METHODS

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

(2013) James E. DiCarlo et al.   NUCLEIC ACIDS RESEARCH

Rational and Evolutionary Engineering Approaches Uncover a Small Set of Genetic Changes Efficient for Rapid Xylose Fermentation in Saccharomyces cerevisiae

(2013) Soo Rin Kim et al.   PLoS One

Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis

(2013) Z. Hou et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Metabolic engineering of Saccharomyces cerevisiae: a key cell factory platform for future biorefineries

(2012) Kuk-Ki Hong et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Engineering microbial factories for synthesis of value-added products

(2011) Jing Du et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY