Efficient CRISPR–Cas9 mediated multiplex genome editing in yeasts

Ct shift: A novel and accurate real-time PCR quantification model for direct comparison of different nucleic acid sequences and its application for transposon quantifications

(2017) Orsolya Kolacsek et al.   GENE

Efficient genome editing by CRISPR/Cas9 with a tRNA-sgRNA fusion in the methylotrophic yeast Ogataea polymorpha

(2017) Minori Numamoto et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

CRISPR/Cas9-coupled recombineering for metabolic engineering of Corynebacterium glutamicum

(2017) Jae Sung Cho et al.   METABOLIC ENGINEERING

Development of a CRISPR/Cas9 genome editing toolbox for Corynebacterium glutamicum

(2017) Jiao Liu et al.   Microbial Cell Factories

Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system

(2017) Feng Peng et al.   Microbial Cell Factories

A Novel Tool for Microbial Genome Editing Using the Restriction-Modification System

(2017) Hua Bai et al.   ACS Synthetic Biology

A New Era of Genome Integration—Simply Cut and Paste!

(2017) Zihe Liu et al.   ACS Synthetic Biology

Bacterial Genome Editing via a Designed Toxin–Antitoxin Cassette

(2017) Jie Wu et al.   ACS Synthetic Biology

Development of a CRISPR-Cas9 Tool Kit for Comprehensive Engineering of Bacillus subtilis

(2016) Adam W. Westbrook et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Construction of plasmids with tunable copy numbers in Saccharomyces cerevisiae and their applications in pathway optimization and multiplex genome integration

(2016) Jiazhang Lian et al.   BIOTECHNOLOGY AND BIOENGINEERING

Synthetic biology and molecular genetics in non-conventional yeasts: Current tools and future advances

(2016) James M. Wagner et al.   FUNGAL GENETICS AND BIOLOGY

A new set of rDNA-NTS-based multiple integrative cassettes for the development of antibiotic-marker-free recombinant yeasts

(2016) Hye Yun Moon et al.   JOURNAL OF BIOTECHNOLOGY

Multiplex gene editing of the Yarrowia lipolytica genome using the CRISPR-Cas9 system

(2016) Shuliang Gao et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

A highly efficient single-step, markerless strategy for multi-copy chromosomal integration of large biochemical pathways in Saccharomyces cerevisiae

(2016) Shuobo Shi et al.   METABOLIC ENGINEERING

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Standardized Markerless Gene Integration for Pathway Engineering in Yarrowia lipolytica

(2016) Cory Schwartz et al.   ACS Synthetic Biology

Expression of a chimeric human/salmon calcitonin gene integrated into the Saccharomyces cerevisiae genome using rDNA sequences as recombination sites

(2015) Hengyi Sun et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Development of the CRISPR/Cas9 System for Targeted Gene Disruption in Aspergillus fumigatus

(2015) Kevin K. Fuller et al.   EUKARYOTIC CELL

De novo production of resveratrol from glucose or ethanol by engineered Saccharomyces cerevisiae

(2015) Mingji Li et al.   METABOLIC ENGINEERING

Efficient Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas

(2015) Andrew A. Horwitz et al.   Cell Systems

Metabolic engineering and classical selection of the methylotrophic thermotolerant yeast Hansenula polymorpha for improvement of high-temperature xylose alcoholic fermentation

(2014) Olena O Kurylenko et al.   Microbial Cell Factories

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

Implementation of the CRISPR-Cas9 system in fission yeast

(2014) Jake Z. Jacobs et al.   Nature Communications

Homology-Integrated CRISPR–Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae

(2014) Zehua Bao et al.   ACS Synthetic Biology

Generation of hepatitis B virus PreS2-S antigen in Hansenula polymorpha

(2014) Xiaowei Xu et al.   VIROLOGICA SINICA

Genome-wide analysis of intraspecific transposon diversity in yeast

(2013) Claudine Bleykasten-Grosshans et al.   BMC GENOMICS

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

(2013) James E. DiCarlo et al.   NUCLEIC ACIDS RESEARCH

Expression of recombinant staphylokinase in the methylotrophic yeast Hansenula polymorpha

(2012) Manal Moussa et al.   BMC BIOTECHNOLOGY

Construction of integrative plasmids suitable for genetic modification of industrial strains of Saccharomyces cerevisiae

(2012) Fernanda Cristina Bezerra Leite et al.   PLASMID

A Mimicking-of-DNA-Methylation-Patterns Pipeline for Overcoming the Restriction Barrier of Bacteria

(2012) Guoqiang Zhang et al.   PLoS Genetics

Optimization of glutathione production in batch and fed-batch cultures by the wild-type and recombinant strains of the methylotrophic yeast Hansenula polymorpha DL-1

(2011) Vira M Ubiyvovk et al.   BMC BIOTECHNOLOGY

Novel genetic tools for Hansenula polymorpha

(2011) Ruchi Saraya et al.   FEMS YEAST RESEARCH

Cocktail δ-integration: a novel method to construct cellulolytic enzyme expression ratio-optimized yeast strains

(2010) Ryosuke Yamada et al.   Microbial Cell Factories

Improved gene disruption method and Cre-loxP mutant system for multiple gene disruptions in Hansenula polymorpha

(2009) Weidong Qian et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Development of strains of the thermotolerant yeast Hansenula polymorpha capable of alcoholic fermentation of starch and xylan

(2009) Andriy Y. Voronovsky et al.   METABOLIC ENGINEERING

New selectable host-marker systems for multiple genetic manipulations based onTRP1, MET2andADE2in the methylotrophic yeastHansenula polymorpha

(2009) Seon Ah Cheon et al.   YEAST

Uricase production by a recombinant Hansenula polymorpha strain harboring Candida utilis uricase gene

(2008) Zhiyu Chen et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Glycoengineering of the methylotrophic yeastHansenula polymorpha for the production of glycoproteins with trimannosyl coreN-glycan by blocking core oligosaccharide assembly

(2008) Doo-Byoung Oh et al.   Biotechnology Journal