Spider Venom: Components, Modes of Action, and Novel Strategies in Transcriptomic and Proteomic Analyses

The Dual Prey-Inactivation Strategy of Spiders—In-Depth Venomic Analysis of Cupiennius salei

(2019) Lucia Kuhn-Nentwig et al.   Toxins

Transcriptomic Analysis of the Spider Venom Gland Reveals Venom Diversity and Species Consanguinity

(2019) Zhaotun Hu et al.   Toxins

Structural Basis of Nav1.7 Inhibition by a Gating-Modifier Spider Toxin

(2019) Hui Xu et al.   CELL

Transcriptome analysis of the spider Phoneutria pertyi venom glands reveals novel venom components for the genus Phoneutria

(2019) Ana L.B. Paiva et al.   TOXICON

Structure–Function and Therapeutic Potential of Spider Venom-Derived Cysteine Knot Peptides Targeting Sodium Channels

(2019) Fernanda C. Cardoso et al.   Frontiers in Pharmacology

Alternative Transcription at Venom Genes and Its Role as a Complementary Mechanism for the Generation of Venom Complexity in the Common House Spider

(2019) Robert A. Haney et al.   Frontiers in Ecology and Evolution

Characterization and remediation of sample index swaps by non-redundant dual indexing on massively parallel sequencing platforms

(2018) Maura Costello et al.   BMC GENOMICS

Gating modifier toxins isolated from spider venom: Modulation of voltage-gated sodium channels and the role of lipid membranes

(2018) Akello J. Agwa et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Transcriptomics-guided bottom-up and top-down venomics of neonate and adult specimens of the arboreal rear-fanged Brown Treesnake, Boiga irregularis , from Guam

(2018) Davinia Pla et al.   Journal of Proteomics

Detection and removal of barcode swapping in single-cell RNA-seq data

(2018) Jonathan A. Griffiths et al.   Nature Communications

Toxins in Drug Discovery and Pharmacology

(2018) et al.   Toxins

True Lies: Using Proteomics to Assess the Accuracy of Transcriptome-Based Venomics in Centipedes Uncovers False Positives and Reveals Startling Intraspecific Variation in Scolopendra subspinipes

(2018) Jennifer Smith et al.   Toxins

Sexually dimorphic venom proteins in long-jawed orb-weaving spiders (Tetragnatha) comprise novel gene families

(2018) Pamela A. Zobel-Thropp et al.   PeerJ

Recent progress and prospects for advancing arachnid genomics

(2018) Jessica E Garb et al.   Current Opinion in Insect Science

Toxin diversity revealed by the venom gland transcriptome of Pardosa pseudoannulata, a natural enemy of several insect pests

(2018) Lixin Huang et al.   Comparative Biochemistry and Physiology D-Genomics & Proteomics

Mechanisms of Channel Block in Calcium-Permeable AMPA Receptors

(2018) Edward C. Twomey et al.   NEURON

An overview of Phoneutria nigriventer spider venom using combined transcriptomic and proteomic approaches

(2018) Marcelo R. V. Diniz et al.   PLoS One

A perspective view of top-down proteomics in snake venom research

(2018) Parviz Ghezellou et al.   RAPID COMMUNICATIONS IN MASS SPECTROMETRY

Structural basis for the modulation of voltage-gated sodium channels by animal toxins

(2018) Huaizong Shen et al.   SCIENCE

The Pfam protein families database in 2019

(2018) Sara El-Gebali et al.   NUCLEIC ACIDS RESEARCH

InterPro in 2019: improving coverage, classification and access to protein sequence annotations

(2018) Alex L Mitchell et al.   NUCLEIC ACIDS RESEARCH

Enhancing the therapeutic potential of peptide toxins

(2017) Raymond S. Norton   Expert Opinion on Drug Discovery

Identification of a precursor processing protease from the spiderCupiennius saleiessential for venom neurotoxin maturation

(2017) Nicolas Langenegger et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Absolute venomics: Absolute quantification of intact venom proteins through elemental mass spectrometry

(2017) Francisco Calderón-Celis et al.   Journal of Proteomics

Strategies in ‘snake venomics’ aiming at an integrative view of compositional, functional, and immunological characteristics of venoms

(2017) Bruno Lomonte et al.   JOURNAL OF VENOMOUS ANIMALS AND TOXINS INCLUDING TROPICAL DISEASES

It is time for top-down venomics

(2017) Rafael D. Melani et al.   JOURNAL OF VENOMOUS ANIMALS AND TOXINS INCLUDING TROPICAL DISEASES

Protein-species quantitative venomics: looking through a crystal ball

(2017) Juan J. Calvete et al.   JOURNAL OF VENOMOUS ANIMALS AND TOXINS INCLUDING TROPICAL DISEASES

A novel post hoc method for detecting index switching finds no evidence for increased switching on the Illumina HiSeq X

(2017) Gregory L. Owens et al.   Molecular Ecology Resources

The Nephila clavipes genome highlights the diversity of spider silk genes and their complex expression

(2017) Paul L Babb et al.   NATURE GENETICS

Potent neuroprotection after stroke afforded by a double-knot spider-venom peptide that inhibits acid-sensing ion channel 1a

(2017) Irène R. Chassagnon et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Identifying different transcribed proteins in the newly described Theraphosidae Pamphobeteus verdolaga

(2017) Sebastian Estrada-Gómez et al.   TOXICON

Spider Neurotoxins, Short Linear Cationic Peptides and Venom Protein Classification Improved by an Automated Competition between Exhaustive Profile HMM Classifiers

(2017) Dominique Koua et al.   Toxins

Venom Profiling of a Population of the Theraphosid Spider Phlogius crassipes Reveals Continuous Ontogenetic Changes from Juveniles through Adulthood

(2017) Renan Santana et al.   Toxins

Venomics of Remipede Crustaceans Reveals Novel Peptide Diversity and Illuminates the Venom’s Biological Role

(2017) Björn von Reumont et al.   Toxins

A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom

(2017) Dilza Trevisan-Silva et al.   Scientific Data

Peptidomic and transcriptomic profiling of four distinct spider venoms

(2017) Vera Oldrati et al.   PLoS One

De novo yeast genome assemblies from MinION, PacBio and MiSeq platforms

(2017) Francesca Giordano et al.   Scientific Reports

Elemental Mass Spectrometry for Absolute Intact Protein Quantification without Protein-Specific Standards: Application to Snake Venomics

(2016) Francisco Calderón-Celis et al.   ANALYTICAL CHEMISTRY

Structure of purotoxin-2 from wolf spider: modular design and membrane-assisted mode of action in arachnid toxins

(2016) P. B. Oparin et al.   BIOCHEMICAL JOURNAL

Membrane-binding properties of gating modifier and pore-blocking toxins: Membrane interaction is not a prerequisite for modification of channel gating

(2016) Evelyne Deplazes et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Efficient enzymatic cyclization of an inhibitory cystine knot-containing peptide

(2016) Soohyun Kwon et al.   BIOTECHNOLOGY AND BIOENGINEERING

Identification and characterization of toxins in the venom gland of the Chinese bird spider,Haplopelma hainanum, by transcriptomic analysis

(2016) Ting-Cai Cheng et al.   Insect Science

Intragenome Diversity of Gene Families Encoding Toxin-like Proteins in Venomous Animals

(2016) Ricardo C. Rodríguez de la Vega et al.   INTEGRATIVE AND COMPARATIVE BIOLOGY

Interaction of Tarantula Venom Peptide ProTx-II with Lipid Membranes Is a Prerequisite for Its Inhibition of Human Voltage-gated Sodium Channel NaV1.7

(2016) Sónia Troeira Henriques et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Top-down venomics of the East African green mamba, Dendroaspis angusticeps , and the black mamba, Dendroaspis polylepis , highlight the complexity of their toxin arsenals

(2016) Daniel Petras et al.   Journal of Proteomics

Mapping Proteoforms and Protein Complexes From King Cobra Venom Using Both Denaturing and Native Top-down Proteomics

(2016) Rafael D. Melani et al.   MOLECULAR & CELLULAR PROTEOMICS

Mass-spectrometric exploration of proteome structure and function

(2016) Ruedi Aebersold et al.   NATURE

Molecular basis of the interaction between gating modifier spider toxins and the voltage sensor of voltage-gated ion channels

(2016) Carus H. Y. Lau et al.   Scientific Reports

Insights into the Hypertensive Effects of Tityus serrulatus Scorpion Venom: Purification of an Angiotensin-Converting Enzyme-Like Peptidase

(2016) Daniela Cajado-Carvalho et al.   Toxins

Latarcins: versatile spider venom peptides

(2015) Peter V. Dubovskii et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Oxford Nanopore sequencing, hybrid error correction, and de novo assembly of a eukaryotic genome

(2015) Sara Goodwin et al.   GENOME RESEARCH

Structural and Functional Diversity of Peptide Toxins from TarantulaHaplopelma hainanum(Ornithoctonus hainana) Venom Revealed by Transcriptomic, Peptidomic, and Patch Clamp Approaches

(2015) Yi-Ya Zhang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Engineering Potent and Selective Analogues of GpTx-1, a Tarantula Venom Peptide Antagonist of the NaV1.7 Sodium Channel

(2015) Justin K. Murray et al.   JOURNAL OF MEDICINAL CHEMISTRY

Venom Proteomics of Indonesian King Cobra, Ophiophagus hannah: Integrating Top-Down and Bottom-Up Approaches

(2015) Daniel Petras et al.   JOURNAL OF PROTEOME RESEARCH

The history of venomous spider identification, venom extraction methods and antivenom production: a long journey at the Butantan Institute, São Paulo, Brazil

(2015) Sylvia M Lucas   JOURNAL OF VENOMOUS ANIMALS AND TOXINS INCLUDING TROPICAL DISEASES

A Comparative Analysis of the Venom Gland Transcriptomes of the Fishing Spiders Dolomedes mizhoanus and Dolomedes sulfurous

(2015) Xunxun Xu et al.   PLoS One

Weaponization of a Hormone: Convergent Recruitment of Hyperglycemic Hormone into the Venom of Arthropod Predators

(2015) Eivind A.B. Undheim et al.   STRUCTURE

A survey of the venom of the spider Lycosa vittata by biochemical, pharmacological and transcriptomic analyses

(2015) Fan Zhang et al.   TOXICON

Mass spectrometry guided venom profiling and bioactivity screening of the Anatolian Meadow Viper, Vipera anatolica

(2015) Bayram Göçmen et al.   TOXICON

Recent Advances in Research on Widow Spider Venoms and Toxins

(2015) Shuai Yan et al.   Toxins

Tarantula toxins use common surfaces for interacting with Kv and ASIC ion channels

(2015) Kanchan Gupta et al.   eLife

Multi-tissue transcriptomics of the black widow spider reveals expansions, co-options, and functional processes of the silk gland gene toolkit

(2014) Thomas H Clarke et al.   BMC GENOMICS

Dramatic expansion of the black widow toxin arsenal uncovered by multi-tissue transcriptomics and venom proteomics

(2014) Robert A Haney et al.   BMC GENOMICS

Genes and evolution of two-domain toxins from lynx spider venom

(2014) Maria Y. Sachkova et al.   FEBS LETTERS

Spider venomics: implications for drug discovery

(2014) Sandy S Pineda et al.   Future Medicinal Chemistry

Structure of the yellow sac spiderCheiracanthium punctoriumgenes provides clues to evolution of insecticidal two-domain knottin toxins

(2014) M. Y. Sachkova et al.   INSECT MOLECULAR BIOLOGY

Multifunctional warheads: Diversification of the toxin arsenal of centipedes via novel multidomain transcripts

(2014) Eivind A.B. Undheim et al.   Journal of Proteomics

High Conopeptide Diversity in Conus tribblei Revealed Through Analysis of Venom Duct Transcriptome Using Two High-Throughput Sequencing Platforms

(2014) Neda Barghi et al.   MARINE BIOTECHNOLOGY

Toxin Diversity Revealed by a Transcriptomic Study of Ornithoctonus huwena

(2014) Yiya Zhang et al.   PLoS One

The Hidden Snake in the Grass: Superior Detection of Snakes in Challenging Attentional Conditions

(2014) Sandra C. Soares et al.   PLoS One

Structural interactions of a voltage sensor toxin with lipid membranes

(2014) Mihaela Mihailescu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Spider genomes provide insight into composition and evolution of venom and silk

(2014) Kristian W. Sanggaard et al.   Nature Communications

Comprehensive analysis of the venom gland transcriptome of the spider Dolomedes fimbriatus

(2014) Sergey A. Kozlov et al.   Scientific Data

Functional evolution of scorpion venom peptides with an inhibitor cystine knot fold

(2013) Bin Gao et al.   BIOSCIENCE REPORTS

The insecticidal potential of venom peptides

(2013) Jennifer J. Smith et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Spider toxins comprising disulfide-rich and linear amphipathic domains: a new class of molecules identified in the lynx spiderOxyopes takobius

(2013) Alexander A. Vassilevski et al.   FEBS Journal

Recruitment and diversification of an ecdysozoan family of neuropeptide hormones for black widow spider venom expression

(2013) Caryn McCowan et al.   GENE

Salivary Antigen-5/CAP Family Members Are Cu2+-dependent Antioxidant Enzymes That Scavenge O2⨪and Inhibit Collagen-induced Platelet Aggregation and Neutrophil Oxidative Burst

(2013) Teresa C. F. Assumpção et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Sea Anemone Peptide with Uncommon β-Hairpin Structure Inhibits Acid-sensing Ion Channel 3 (ASIC3) and Reveals Analgesic Activity

(2013) Dmitry I. Osmakov et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Opening the Shaker K+channel with hanatoxin

(2013) Mirela Milescu et al.   JOURNAL OF GENERAL PHYSIOLOGY

Spit and Venom from Scytodes Spiders: A Diverse and Distinct Cocktail

(2013) Pamela A. Zobel-Thropp et al.   JOURNAL OF PROTEOME RESEARCH

Molecular Evolution of α-Latrotoxin, the Exceptionally Potent Vertebrate Neurotoxin in Black Widow Spider Venom

(2013) Jessica E. Garb et al.   MOLECULAR BIOLOGY AND EVOLUTION

Potency optimization of Huwentoxin-IV on hNav1.7: A neurotoxin TTX-S sodium-channel antagonist from the venom of the Chinese bird-eating spider Selenocosmia huwena

(2013) Jefferson D. Revell et al.   PEPTIDES

SVM-Based Prediction of Propeptide Cleavage Sites in Spider Toxins Identifies Toxin Innovation in an Australian Tarantula

(2013) Emily S. W. Wong et al.   PLoS One

The Venom Gland Transcriptome of Latrodectus tredecimguttatus Revealed by Deep Sequencing and cDNA Library Analysis

(2013) Quanze He et al.   PLoS One

Synthesis and biological characterization of synthetic analogs of Huwentoxin-IV (Mu-theraphotoxin-Hh2a), a neuronal tetrodotoxin-sensitive sodium channel inhibitor

(2013) Meichun Deng et al.   TOXICON

Transcriptome analysis of venom glands from a single fishing spider Dolomedes mizhoanus

(2013) Liping Jiang et al.   TOXICON

A Proteomics and Transcriptomics Investigation of the Venom from the Barychelid Spider Trittame loki (Brush-Foot Trapdoor)

(2013) Eivind Undheim et al.   Toxins

Evolution Stings: The Origin and Diversification of Scorpion Toxin Peptide Scaffolds

(2013) Kartik Sunagar et al.   Toxins

Spider-Venom Peptides: Structure, Pharmacology, and Potential for Control of Insect Pests

(2012) Glenn F. King et al.   Annual Review of Entomology

A Venom-derived Neurotoxin, CsTx-1, from the SpiderCupiennius saleiExhibits Cytolytic Activities

(2012) Lucia Kuhn-Nentwig et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Comparison of Next-Generation Sequencing Systems

(2012) Lin Liu et al.   JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY

A combined de novo protein sequencing and cDNA library approach to the venomic analysis of Chinese spider Araneus ventricosus

(2012) Zhigui Duan et al.   Journal of Proteomics

Structural plasticity and dynamic selectivity of acid-sensing ion channel–spider toxin complexes

(2012) Isabelle Baconguis et al.   NATURE

Improving PacBio Long Read Accuracy by Short Read Alignment

(2012) Kin Fai Au et al.   PLoS One

Sphingomyelinase D in sicariid spider venom is a potent insecticidal toxin

(2012) Pamela A. Zobel-Thropp et al.   TOXICON

The UniProtKB/Swiss-Prot Tox-Prot program: A central hub of integrated venom protein data

(2012) Florence Jungo et al.   TOXICON

Complex cocktails: the evolutionary novelty of venoms

(2012) Nicholas R. Casewell et al.   TRENDS IN ECOLOGY & EVOLUTION

Structure of the Acid-sensing ion channel 1 in complex with the gating modifier Psalmotoxin 1

(2012) Roger J.P. Dawson et al.   Nature Communications

Spider-Venom Peptides as Bioinsecticides

(2012) Monique J. Windley et al.   Toxins

Research strategies to improve snakebite treatment: Challenges and progress

(2011) Robert A. Harrison et al.   Journal of Proteomics

Double indexing overcomes inaccuracies in multiplex sequencing on the Illumina platform

(2011) Martin Kircher et al.   NUCLEIC ACIDS RESEARCH

Cupiennin 1a exhibits a remarkably broad, non-stereospecific cytolytic activity on bacteria, protozoan parasites, insects, and human cancer cells

(2010) Lucia Kuhn-Nentwig et al.   AMINO ACIDS

Molecular diversity of spider venom

(2010) A. A. Vassilevski et al.   BIOCHEMISTRY-MOSCOW

A Bivalent Tarantula Toxin Activates the Capsaicin Receptor, TRPV1, by Targeting the Outer Pore Domain

(2010) Christopher J. Bohlen et al.   CELL

Venom components from Citharischius crawshayi spider (Family Theraphosidae): exploring transcriptome, venomics, and function

(2010) Elia Diego-García et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Mesothelae have venom glands

(2010) Rainer Foelix et al.   JOURNAL OF ARACHNOLOGY

Use of Venom Peptides to Probe Ion Channel Structure and Function

(2010) Sébastien Dutertre et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Novel Class of Spider Toxin

(2010) Alexander A. Vassilevski et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Molecular Diversification of Peptide Toxins from the TarantulaHaplopelma hainanum(Ornithoctonus hainana) Venom Based on Transcriptomic, Peptidomic, and Genomic Analyses

(2010) Xing Tang et al.   JOURNAL OF PROTEOME RESEARCH

Snake Venomics of African Spitting Cobras: Toxin Composition and Assessment of Congeneric Cross-Reactivity of the Pan-African EchiTAb-Plus-ICP Antivenom by Antivenomics and Neutralization Approaches

(2010) Daniel Petras et al.   JOURNAL OF PROTEOME RESEARCH

Molecular Diversity and Functional Evolution of Scorpion Potassium Channel Toxins

(2010) Shunyi Zhu et al.   MOLECULAR & CELLULAR PROTEOMICS

A novel expression profile of the Loxosceles intermedia spider venomous gland revealed by transcriptome analysis

(2010) Luiza Helena Gremski et al.   Molecular BioSystems

ArachnoServer 2.0, an updated online resource for spider toxin sequences and structures

(2010) V. Herzig et al.   NUCLEIC ACIDS RESEARCH

Immunome and venome of Bothrops jararacussu: A proteomic approach to study the molecular immunology of snake toxins

(2010) Carlos Correa-Netto et al.   TOXICON

Diversity of conotoxin types from Conus californicus reflects a diversity of prey types and a novel evolutionary history

(2010) C.A. Elliger et al.   TOXICON

Spider-Venom Peptides as Therapeutics

(2010) Natalie J. Saez et al.   Toxins

Processing of peptide and hormone precursors at the dibasic cleavage sites

(2009) Mohamed Rholam et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Herbivory in a spider through exploitation of an ant–plant mutualism

(2009) Christopher J. Meehan et al.   CURRENT BIOLOGY

Interactions between lipids and voltage sensor paddles detected with tarantula toxins

(2009) Mirela Milescu et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Geographic Variation in Venom Allelic Composition and Diets of the Widespread Predatory Marine Gastropod Conus ebraeus

(2009) Thomas F. Duda et al.   PLoS One

Coevolution of diet and prey-specific venom activity supports the role of selection in snake venom evolution

(2009) A. Barlow et al.   PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Functional basis of a molecular adaptation: Prey-specific toxic effects of venom from Sistrurus rattlesnakes

(2009) H. Lisle Gibbs et al.   TOXICON

Anti-arthritic activity of Indian monocellate cobra (Naja kaouthia) venom on adjuvant induced arthritis

(2009) Antony Gomes et al.   TOXICON

Transcriptome analysis of the venom glands of the Chinese wolf spider Lycosa singoriensis

(2009) Yongqun Zhang et al.   ZOOLOGY

Epidemiology of scorpionism: A global appraisal

(2008) J.-P. Chippaux et al.   ACTA TROPICA

Cyto-insectotoxins, a novel class of cytolytic and insecticidal peptides from spider venom

(2008) Alexander A. Vassilevski et al.   BIOCHEMICAL JOURNAL

Three-Dimensional Structure/Hydrophobicity of Latarcins Specifies Their Mode of Membrane Activity†,‡

(2008) Peter V. Dubovskii et al.   BIOCHEMISTRY

Transcriptome analysis of Loxosceles laeta (Araneae, Sicariidae) spider venomous gland using expressed sequence tags

(2008) Matheus de F Fernandes-Pedrosa et al.   BMC GENOMICS

Structural and functional characterization of two genetically related meucin peptides highlights evolutionary divergence and convergence in antimicrobial peptides

(2008) Bin Gao et al.   FASEB JOURNAL

Proteomic analysis ofLatrodectus tredecimguttatusvenom for uncovering potential latrodectism-related proteins

(2008) Zhigui Duan et al.   JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY

Venomics: unravelling the complexity of animal venoms with mass spectrometry

(2008) P. Escoubas et al.   JOURNAL OF MASS SPECTROMETRY

Molecular Evolution, Functional Variation, and Proposed Nomenclature of the Gene Family That Includes Sphingomyelinase D in Sicariid Spider Venoms

(2008) G. J. Binford et al.   MOLECULAR BIOLOGY AND EVOLUTION

NMR-spectroscopic screening of spider venom reveals sulfated nucleosides as major components for the brown recluse and related species

(2008) F. C. Schroeder et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural analysis of the venom glands of the armed spider Phoneutria nigriventer (Keyserling, 1891): Microanatomy, fine structure and confocal observations

(2008) Luciana Maria Silva et al.   TOXICON

Molecular diversification based on analysis of expressed sequence tags from the venom glands of the Chinese bird spider Ornithoctonus huwena,

(2008) Liping Jiang et al.   TOXICON

Transcriptome analysis revealed novel possible venom components and cellular processes of the tarantula Chilobrachys jingzhao venom gland

(2008) Jinjun Chen et al.   TOXICON

A rational nomenclature for naming peptide toxins from spiders and other venomous animals

(2008) Glenn F. King et al.   TOXICON