Ultra-high throughput functional enrichment of large monoamine oxidase (MAO-N) libraries by fluorescence activated cell sorting

Mapping the substrate scope of monoamine oxidase (MAO-N) as a synthetic tool for the enantioselective synthesis of chiral amines

(2018) Susanne Herter et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Biocatalysts for the pharmaceutical industry created by structure-guided directed evolution of stereoselective enzymes

(2018) Guangyue Li et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Simultaneous engineering of an enzyme's entrance tunnel and active site: the case of monoamine oxidase MAO-N

(2017) Guangyue Li et al.   Chemical Science

Bacterial Microcolonies in Gel Beads for High-Throughput Screening of Libraries in Synthetic Biology

(2017) José M. Duarte et al.   ACS Synthetic Biology

Characterization of the effects of n-butanol on the cell envelope of E. coli

(2016) Eugene Fletcher et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Directed evolution and synthetic biology applications to microbial systems

(2016) Marcelo C Bassalo et al.   CURRENT OPINION IN BIOTECHNOLOGY

Ultrahigh-throughput–directed enzyme evolution by absorbance-activated droplet sorting (AADS)

(2016) Fabrice Gielen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Catalytic Asymmetric Reduction of Difficult-to-Reduce Ketones: Triple-Code Saturation Mutagenesis of an Alcohol Dehydrogenase

(2016) Zhoutong Sun et al.   ACS Catalysis

Semi-rational Directed Evolution of Monoamine Oxidase for Kinetic Resolution of rac-Mexiletine

(2015) Zhenming Chen et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Iterative key-residues interrogation of a phytase with thermostability increasing substitutions identified in directed evolution

(2015) Amol V. Shivange et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Simultaneous Use of in Silico Design and a Correlated Mutation Network as a Tool To Efficiently Guide Enzyme Engineering

(2015) Alberto Nobili et al.   CHEMBIOCHEM

Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently

(2015) Andrew Currin et al.   CHEMICAL SOCIETY REVIEWS

Improving and repurposing biocatalysts via directed evolution

(2015) Carl A Denard et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Microbial individuality: how single-cell heterogeneity enables population level strategies

(2015) Bruno MC Martins et al.   CURRENT OPINION IN MICROBIOLOGY

High-throughput analysis and protein engineering using microcapillary arrays

(2015) Bob Chen et al.   Nature Chemical Biology

Membrane transporter engineering in industrial biotechnology and whole cell biocatalysis

(2015) Douglas B. Kell et al.   TRENDS IN BIOTECHNOLOGY

Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics

(2015) Pierre-Yves Colin et al.   Nature Communications

Deracemization By Simultaneous Bio-oxidative Kinetic Resolution and Stereoinversion

(2014) Joerg H. Schrittwieser et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Immobilised whole-cell recombinant monoamine oxidase biocatalysis

(2014) Petra Zajkoska et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Directed evolution of a cellodextrin transporter for improved biofuel production under anaerobic conditions inSaccharomyces cerevisiae

(2014) Jiazhang Lian et al.   BIOTECHNOLOGY AND BIOENGINEERING

Ultra-High-Throughput Screening Method for the Directed Evolution of Glucose Oxidase

(2014) Raluca Ostafe et al.   CHEMISTRY & BIOLOGY

High Throughput Screening and Selection Methods for Directed Enzyme Evolution

(2014) Han Xiao et al.   INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH

SpeedyGenes: an improved gene synthesis method for the efficient production of error-corrected, synthetic protein libraries for directed evolution

(2014) Andrew Currin et al.   PROTEIN ENGINEERING DESIGN & SELECTION

How drugs get into cells: tested and testable predictions to help discriminate between transporter-mediated uptake and lipoidal bilayer diffusion

(2014) Douglas B. Kell et al.   Frontiers in Pharmacology

A universal flow cytometry assay for screening carbohydrate-active enzymes using glycan microspheres

(2013) Aarthi Chandrasekaran et al.   CHEMICAL COMMUNICATIONS

Engineering an Enantioselective Amine Oxidase for the Synthesis of Pharmaceutical Building Blocks and Alkaloid Natural Products

(2013) Diego Ghislieri et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Biocatalytic Approaches to the Synthesis of Enantiomerically Pure Chiral Amines

(2013) Diego Ghislieri et al.   TOPICS IN CATALYSIS

Vanadium bromoperoxidase-coupled fluorescent assay for flow cytometry sorting of glucose oxidase gene libraries in double emulsions

(2012) Radivoje Prodanovic et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Scientific discovery as a combinatorial optimisation problem: How best to navigate the landscape of possible experiments?

(2012) Douglas B. Kell   BIOESSAYS

Directed Evolution of Highly Selective Proteases by Using a Novel FACS-Based Screen that Capitalizes on the p53 Regulator MDM2

(2012) Tae Hyeon Yoo et al.   CHEMBIOCHEM

Directed Evolution of the Enzyme Monoamine Oxidase (MAO-N): Highly Efficient Chemo-enzymatic Deracemisation of the Alkaloid (±)-Crispine A

(2012) Ian Rowles et al.   ChemCatChem

Thioflavin-S staining coupled to flow cytometry. A screening tool to detect in vivo protein aggregation

(2012) Alba Espargaró et al.   Molecular BioSystems

Engineering the third wave of biocatalysis

(2012) U. T. Bornscheuer et al.   NATURE

Directed evolution of a highly active Yersinia mollaretii phytase

(2011) Amol V. Shivange et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Methods for detection and measurement of hydrogen peroxide inside and outside of cells

(2010) Sue Goo Rhee et al.   MOLECULES AND CELLS

Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture

(2010) C. K. Savile et al.   SCIENCE

Ultrahigh-Throughput FACS-Based Screening for Directed Enzyme Evolution

(2009) Guangyu Yang et al.   CHEMBIOCHEM

A review of the use of genetically engineered enzymes in electrochemical biosensors

(2009) Mònica Campàs et al.   SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY

Directed Evolution of Methanococcus jannaschii Citramalate Synthase for Biosynthesis of 1-Propanol and 1-Butanol by Escherichia coli

(2008) S. Atsumi et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

The Structure of Monoamine Oxidase from Aspergillus niger Provides a Molecular Context for Improvements in Activity Obtained by Directed Evolution

(2008) Kate E. Atkin et al.   JOURNAL OF MOLECULAR BIOLOGY