Efficient laboratory evolution of computationally designed enzymes with low starting activities using fluorescence-activated droplet sorting

Fast Knoevenagel Condensations Catalyzed by an Artificial Schiff-Base-Forming Enzyme

(2016) Xavier Garrabou et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Emergence of a catalytic tetrad during evolution of a highly active artificial aldolase

(2016) Richard Obexer et al.   Nature Chemistry

High-throughput analysis and protein engineering using microcapillary arrays

(2015) Bob Chen et al.   Nature Chemical Biology

Methods for the directed evolution of proteins

(2015) Michael S. Packer et al.   NATURE REVIEWS GENETICS

Functional Mapping of Protein-Protein Interactions in an Enzyme Complex by Directed Evolution

(2015) Kathrin Roderer et al.   PLoS One

One in a Million: Flow Cytometric Sorting of Single Cell-Lysate Assays in Monodisperse Picolitre Double Emulsion Droplets for Directed Evolution

(2014) Anastasia Zinchenko et al.   ANALYTICAL CHEMISTRY

Droplet-Based Microfluidics Platform for Ultra-High-Throughput Bioprospecting of Cellulolytic Microorganisms

(2014) Majdi Najah et al.   CHEMISTRY & BIOLOGY

Ultra-High-Throughput Screening Method for the Directed Evolution of Glucose Oxidase

(2014) Raluca Ostafe et al.   CHEMISTRY & BIOLOGY

de novo computational enzyme design

(2014) Alexandre Zanghellini   CURRENT OPINION IN BIOTECHNOLOGY

Evolution of enzyme catalysts caged in biomimetic gel-shell beads

(2014) Martin Fischlechner et al.   Nature Chemistry

Impact of scaffold rigidity on the design and evolution of an artificial Diels-Alderase

(2014) N. Preiswerk et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Computational Enzyme Design

(2013) Gert Kiss et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Design of Protein Catalysts

(2013) Donald Hilvert   Annual Review of Biochemistry

Exploration of Alternate Catalytic Mechanisms and Optimization Strategies for Retroaldolase Design

(2013) Sinisa Bjelic et al.   JOURNAL OF MOLECULAR BIOLOGY

Precision is essential for efficient catalysis in an evolved Kemp eliminase

(2013) Rebecca Blomberg et al.   NATURE

Evolution of a designed retro-aldolase leads to complete active site remodeling

(2013) Lars Giger et al.   Nature Chemical Biology

Single-cell analysis and sorting using droplet-based microfluidics

(2013) Linas Mazutis et al.   Nature Protocols

Picoliter Cell Lysate Assays in Microfluidic Droplet Compartments for Directed Enzyme Evolution

(2012) Balint Kintses et al.   CHEMISTRY & BIOLOGY

A completely in vitro ultrahigh-throughput droplet-based microfluidic screening system for protein engineering and directed evolution

(2012) Ali Fallah-Araghi et al.   LAB ON A CHIP

Iterative approach to computational enzyme design

(2012) H. K. Privett et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Bridging the gaps in design methodologies by evolutionary optimization of the stability and proficiency of designed Kemp eliminase KE59

(2012) O. Khersonsky et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Robust design and optimization of retroaldol enzymes

(2012) Eric A. Althoff et al.   PROTEIN SCIENCE

Optimization of the In-Silico-Designed Kemp Eliminase KE70 by Computational Design and Directed Evolution

(2011) Olga Khersonsky et al.   JOURNAL OF MOLECULAR BIOLOGY

De Novo Enzyme Design Using Rosetta3

(2011) Florian Richter et al.   PLoS One

Miniaturization and Parallelization of Biological and Chemical Assays in Microfluidic Devices

(2010) Saurabh Vyawahare et al.   CHEMISTRY & BIOLOGY

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

(2010) J. J. Agresti et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Computational Design of an Enzyme Catalyst for a Stereoselective Bimolecular Diels-Alder Reaction

(2010) J. B. Siegel et al.   SCIENCE

Palladium-catalyzed Substitution of Ketone or Aldehyde Bearing Aryl Triflates by Amines or Amides

(2009) Xiaochun TAO et al.   CHINESE JOURNAL OF CHEMISTRY

Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity

(2009) Jean-Christophe Baret et al.   LAB ON A CHIP

Biocompatible surfactants for water-in-fluorocarbon emulsions

(2008) C. Holtze et al.   LAB ON A CHIP

Kemp elimination catalysts by computational enzyme design

(2008) Daniela Röthlisberger et al.   NATURE

De Novo Computational Design of Retro-Aldol Enzymes

(2008) L. Jiang et al.   SCIENCE