3D Cell-Based Assays for Drug Screens: Challenges in Imaging, Image Analysis, and High-Content Analysis

A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity

(2018) Norman Sachs et al.   CELL

Disease Modeling Using 3D Organoids Derived from Human Induced Pluripotent Stem Cells

(2018) Beatrice Ho et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Imaging organoids: a bright future ahead

(2018) Anne C Rios et al.   NATURE METHODS

Patient-derived organoids model treatment response of metastatic gastrointestinal cancers

(2018) Georgios Vlachogiannis et al.   SCIENCE

Silencing NIK potentiates anti-VEGF therapy in a novel 3D model of colorectal cancer angiogenesis

(2018) Chrissta X. Maracle et al.   Oncotarget

Automated brightfield morphometry of 3D organoid populations by OrganoSeg

(2018) Michael A. Borten et al.   Scientific Reports

A high-throughput imaging and nuclear segmentation analysis protocol for cleared 3D culture models

(2018) Molly E. Boutin et al.   Scientific Reports

Stability of gene expression by primary bronchial epithelial cells over increasing passage number

(2018) Stephen R. Reeves et al.   BMC Pulmonary Medicine

Three-Dimensional in Vitro Cell Culture Models in Drug Discovery and Drug Repositioning

(2018) Sigrid A. Langhans   Frontiers in Pharmacology

A Novel Three-Dimensional Immune Oncology Model for High-Throughput Testing of Tumoricidal Activity

(2018) Hilary Sherman et al.   Frontiers in Immunology

Different culture media modulate growth, heterogeneity, and senescence in human mammary epithelial cell cultures

(2018) Jonathan K. Lee et al.   PLoS One

Patient-derived xenografts undergo mouse-specific tumor evolution

(2017) Uri Ben-David et al.   NATURE GENETICS

Improving your four-dimensional image: traveling through a decade of light-sheet-based fluorescence microscopy research

(2017) Frederic Strobl et al.   Nature Protocols

Imaging tissue-mimic with light sheet microscopy: A comparative guideline

(2017) Jordi Andilla et al.   Scientific Reports

Development of a 3D angiogenesis model to study tumour – endothelial cell interactions and the effects of anti-angiogenic drugs

(2017) Arno Amann et al.   Scientific Reports

Development of hydrogels for regenerative engineering

(2017) Xiaofei Guan et al.   Biotechnology Journal

Image-based ex-vivo drug screening for patients with aggressive haematological malignancies: interim results from a single-arm, open-label, pilot study

(2017) Berend Snijder et al.   Lancet Haematology

Drug Discovery Approaches Utilizing Three-Dimensional Cell Culture

(2016) Sarah-Louise Ryan et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

High throughput physiological screening of iPSC-derived cardiomyocytes for drug development

(2016) Juan C. del Álamo et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Collagen–Poly(N-isopropylacrylamide) Hydrogels with Tunable Properties

(2016) Amanda L. Barnes et al.   BIOMACROMOLECULES

A 3D in vitro model of patient-derived prostate cancer xenograft for controlled interrogation of in vivo tumor-stromal interactions

(2016) Eliza L.S. Fong et al.   BIOMATERIALS

A novel three-dimensional heterotypic spheroid model for the assessment of the activity of cancer immunotherapy agents

(2016) Sylvia Herter et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

Modeling Development and Disease with Organoids

(2016) Hans Clevers   CELL

Organoid Culture of Isolated Cells from Patient-derived Tissues with Colorectal Cancer

(2016) Ai-Wen Wu et al.   CHINESE MEDICAL JOURNAL

Engineering approaches to study fibrosis in 3-D in vitro systems

(2016) Ana M Porras et al.   CURRENT OPINION IN BIOTECHNOLOGY

Organoids: A historical perspective of thinking in three dimensions

(2016) Marina Simian et al.   JOURNAL OF CELL BIOLOGY

High-throughput imaging: Focusing in on drug discovery in 3D

(2016) Linfeng Li et al.   METHODS

Designer matrices for intestinal stem cell and organoid culture

(2016) Nikolce Gjorevski et al.   NATURE

Defined three-dimensional microenvironments boost induction of pluripotency

(2016) Massimiliano Caiazzo et al.   NATURE MATERIALS

Screening out irrelevant cell-based models of disease

(2016) Peter Horvath et al.   NATURE REVIEWS DRUG DISCOVERY

SAMA: A Method for 3D Morphological Analysis

(2016) Tessie Paulose et al.   PLoS One

Tumor-induced remote ECM network orientation steers angiogenesis

(2016) Hayri E. Balcioglu et al.   Scientific Reports

BioSig3D: High Content Screening of Three-Dimensional Cell Culture Models

(2016) Cemal Cagatay Bilgin et al.   PLoS One

High-Content Assays for Characterizing the Viability and Morphology of 3D Cancer Spheroid Cultures

(2015) Oksana Sirenko et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Alzheimer's in 3D culture: Challenges and perspectives

(2015) Carla D'Avanzo et al.   BIOESSAYS

Prospective Derivation of a Living Organoid Biobank of Colorectal Cancer Patients

(2015) Marc van de Wetering et al.   CELL

Identification of anti-tumour biologics using primary tumour models, 3-D phenotypic screening and image-based multi-parametric profiling

(2015) Alan M. Sandercock et al.   Molecular Cancer

Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis

(2015) Minoru Takasato et al.   NATURE

3D in vitro modeling of the central nervous system

(2015) Amy M. Hopkins et al.   PROGRESS IN NEUROBIOLOGY

Strategies for improving the physiological relevance of human engineered tissues

(2015) Rosalyn D. Abbott et al.   TRENDS IN BIOTECHNOLOGY

High-Throughput Screening to Identify Compounds That Increase Fragile X Mental Retardation Protein Expression in Neural Stem Cells Differentiated From Fragile X Syndrome Patient-Derived Induced Pluripotent Stem Cells

(2015) Daman Kumari et al.   Stem Cells Translational Medicine

Effect of Fibroblasts on Epithelial Regeneration on the Surface of a Bioengineered Trachea

(2014) Yukio Nomoto et al.   ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY

Three-Dimensional Cell Culture Systems and Their Applications in Drug Discovery and Cell-Based Biosensors

(2014) Rasheena Edmondson et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Organoid cultures for the analysis of cancer phenotypes

(2014) Norman Sachs et al.   CURRENT OPINION IN GENETICS & DEVELOPMENT

Integrins and epithelial cell polarity

(2014) Jessica L. Lee et al.   JOURNAL OF CELL SCIENCE

Hydrogel-Based 3D Model of Patient-Derived Prostate Xenograft Tumors Suitable for Drug Screening

(2014) Eliza L. S. Fong et al.   MOLECULAR PHARMACEUTICS

3-Dimensional Culture Systems for Anti-Cancer Compound Profiling and High-Throughput Screening Reveal Increases in EGFR Inhibitor-Mediated Cytotoxicity Compared to Monolayer Culture Systems

(2014) Amy L. Howes et al.   PLoS One

Ultra High Content Image Analysis and Phenotype Profiling of 3D Cultured Micro-Tissues

(2014) Zi Di et al.   PLoS One

 1 Integrin Inhibition Elicits a Prometastatic Switch Through the TGF -miR-200-ZEB Network in E-Cadherin-Positive Triple-Negative Breast Cancer

(2014) H. H. Truong et al.   Science Signaling

Tissue-engineered kidney disease models

(2013) Teresa M. DesRochers et al.   ADVANCED DRUG DELIVERY REVIEWS

Phenotypic vs. Target-Based Drug Discovery for First-in-Class Medicines

(2013) D C Swinney   CLINICAL PHARMACOLOGY & THERAPEUTICS

Phenotypic screens as a renewed approach for drug discovery

(2013) Wei Zheng et al.   DRUG DISCOVERY TODAY

3Din vitrotissue models and their potential for drug screening

(2013) Lauren Kimlin et al.   Expert Opinion on Drug Discovery

Functional 3-D cardiac co-culture model using bioactive chitosan nanofiber scaffolds

(2012) Ali Hussain et al.   BIOTECHNOLOGY AND BIOENGINEERING

Induced Pluripotent Stem Cells as a Disease Modeling and Drug Screening Platform

(2012) Antje D. Ebert et al.   JOURNAL OF CARDIOVASCULAR PHARMACOLOGY

Deconstructing the third dimension – how 3D culture microenvironments alter cellular cues

(2012) Brendon M. Baker et al.   JOURNAL OF CELL SCIENCE

NIH Image to ImageJ: 25 years of image analysis

(2012) Caroline A Schneider et al.   NATURE METHODS

ECM microenvironment regulates collective migration and local dissemination in normal and malignant mammary epithelium

(2012) K.-V. Nguyen-Ngoc et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Hydrogels for in vivo -like three-dimensional cellular studies

(2012) Ross DeVolder et al.   Wiley Interdisciplinary Reviews-Systems Biology and Medicine

Single cell transcriptional profiling reveals heterogeneity of human induced pluripotent stem cells

(2011) Kazim H. Narsinh et al.   JOURNAL OF CLINICAL INVESTIGATION

In vitro three-dimensional (3D) models in cancer research: An update

(2011) Lauren C. Kimlin et al.   MOLECULAR CARCINOGENESIS

How were new medicines discovered?

(2011) David C. Swinney et al.   NATURE REVIEWS DRUG DISCOVERY

The beautiful cell: high-content screening in drug discovery

(2010) Marc Bickle   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Bioactive hydrogels made from step-growth derived PEG–peptide macromers

(2010) Jordan S. Miller et al.   BIOMATERIALS

Modeling inherited metabolic disorders of the liver using human induced pluripotent stem cells

(2010) S. Tamir Rashid et al.   JOURNAL OF CLINICAL INVESTIGATION

Measurement of mechanical tractions exerted by cells in three-dimensional matrices

(2010) Wesley R Legant et al.   NATURE METHODS

Tissue-Engineered Three-Dimensional In Vitro Models for Normal and Diseased Kidney

(2010) Balajikarthick Subramanian et al.   TISSUE ENGINEERING PART A

Cell-Matrix Interactions in Mammary Gland Development and Breast Cancer

(2010) J. Muschler et al.   Cold Spring Harbor Perspectives in Biology

Hydrogels as extracellular matrix mimics for 3D cell culture

(2009) Mark W. Tibbitt et al.   BIOTECHNOLOGY AND BIOENGINEERING

In Vitro 3D Model for Human Vascularized Adipose Tissue

(2009) Jennifer H. Kang et al.   TISSUE ENGINEERING PART A

Disease-Specific Induced Pluripotent Stem Cells

(2008) In-Hyun Park et al.   CELL

Cell-Polarity Dynamics Controls the Mechanism of Lumen Formation in Epithelial Morphogenesis

(2008) Fernando Martín-Belmonte et al.   CURRENT BIOLOGY

Stem-cell-based therapy and lessons from the heart

(2008) Robert Passier et al.   NATURE

Induced Pluripotent Stem Cells Generated from Patients with ALS Can Be Differentiated into Motor Neurons

(2008) J. T. Dimos et al.   SCIENCE