Novel bioactive natural products from bacteria via bioprospecting, genome mining and metabolic engineering

Discovery of the Cyclic Lipopeptide Gacamide A by Genome Mining and Repair of the Defective GacA Regulator in Pseudomonas fluorescens Pf0-1

(2019) Gahzaleh Jahanshah et al.   JOURNAL OF NATURAL PRODUCTS

Rare taxa and dark microbial matter: novel bioactive actinobacteria abound in Atacama Desert soils

(2018) Michael Goodfellow et al.   ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY

Identification and heterologous expression of the actinoallolide biosynthetic gene cluster

(2018) Yuki Inahashi et al.   JOURNAL OF ANTIBIOTICS

Hybrid MS/NMR methods on the prioritization of natural products: Applications in drug discovery

(2018) Nelson G.M. Gomes et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Design, development and application of whole-cell based antibiotic-specific biosensor

(2018) Yuriy Rebets et al.   METABOLIC ENGINEERING

Heterologous expression and antitumor activity analysis of syringolin from Pseudomonas syringae pv. syringae B728a

(2018) Fan Huang et al.   Microbial Cell Factories

Characterization and heterologous expression of the neoabyssomicin/abyssomicin biosynthetic gene cluster from Streptomyces koyangensis SCSIO 5802

(2018) Jiajia Tu et al.   Microbial Cell Factories

Discovery, Biosynthesis, and Heterologous Production of Streptoseomycin, an Anti-Microaerophilic Bacteria Macrodilactone

(2018) Bo Zhang et al.   ORGANIC LETTERS

Self-resistance guided genome mining uncovers new topoisomerase inhibitors from myxobacteria

(2018) Fabian Panter et al.   Chemical Science

A standardized workflow for submitting data to the Minimum Information about a Biosynthetic Gene cluster (MIBiG) repository: prospects for research-based educational experiences

(2018) Samuel C. Epstein et al.   Standards in Genomic Sciences

Bioprospecting Deep-Sea Actinobacteria for Novel Anti-infective Natural Products

(2018) Dongbo Xu et al.   Frontiers in Microbiology

Genomics-Based Insights Into the Biosynthesis and Unusually High Accumulation of Free Fatty Acids by Streptomyces sp. NP10

(2018) Olha Schneider et al.   Frontiers in Microbiology

High-Yield Production of Herbicidal Thaxtomins and Thaxtomin Analogs in a Nonpathogenic Streptomyces Strain

(2018) Guangde Jiang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Generation of a cluster-free Streptomyces albus chassis strains for improved heterologous expression of secondary metabolite clusters

(2018) Maksym Myronovskyi et al.   METABOLIC ENGINEERING

Genome Mining of Streptomyces sp. YIM 130001 Isolated From Lichen Affords New Thiopeptide Antibiotic

(2018) Olha Schneider et al.   Frontiers in Microbiology

PCR-Independent Method of Transformation-Associated Recombination Reveals the Cosmomycin Biosynthetic Gene Cluster in an Ocean Streptomycete

(2017) Charles B. Larson et al.   JOURNAL OF NATURAL PRODUCTS

Identification and heterologous expression of the kocurin biosynthetic gene cluster

(2017) Luis Linares-Otoya et al.   MICROBIOLOGY-SGM

Comparative mass spectrometry-based metabolomics strategies for the investigation of microbial secondary metabolites

(2017) Brett C. Covington et al.   NATURAL PRODUCT REPORTS

Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea

(2017) Robert M Bowers et al.   NATURE BIOTECHNOLOGY

CRISPR–Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters

(2017) Mingzi M Zhang et al.   Nature Chemical Biology

Development of a Biosensor Concept to Detect the Production of Cluster-Specific Secondary Metabolites

(2017) Yi-Qian Sun et al.   ACS Synthetic Biology

Identification by Genome Mining of a Type I Polyketide Gene Cluster from Streptomyces argillaceus Involved in the Biosynthesis of Pyridine and Piperidine Alkaloids Argimycins P

(2017) Suhui Ye et al.   Frontiers in Microbiology

Marizomib irreversibly inhibits proteasome to overcome compensatory hyperactivation in multiple myeloma and solid tumour patients

(2016) Nancy Levin et al.   BRITISH JOURNAL OF HAEMATOLOGY

Natural Products as Sources of New Drugs from 1981 to 2014

(2016) David J. Newman et al.   JOURNAL OF NATURAL PRODUCTS

Biosynthetic engineering of nonribosomal peptide synthetases

(2016) Hajo Kries   JOURNAL OF PEPTIDE SCIENCE

Dual control system – A novel scaffolding architecture of an inducible regulatory device for the precise regulation of gene expression

(2016) L. Horbal et al.   METABOLIC ENGINEERING

Activation of a plasmid-situated type III PKS gene cluster by deletion of a wbl gene in deepsea-derived Streptomyces somaliensis SCSIO ZH66

(2016) Huiming Huang et al.   Microbial Cell Factories

New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters

(2016) Yunzi Luo et al.   NATURAL PRODUCT REPORTS

Genetic engineering of modular PKSs: from combinatorial biosynthesis to synthetic biology

(2016) Kira J. Weissman   NATURAL PRODUCT REPORTS

Human commensals producing a novel antibiotic impair pathogen colonization

(2016) Alexander Zipperer et al.   NATURE

CasHRA (Cas9-facilitated Homologous Recombination Assembly) method of constructing megabase-sized DNA

(2016) Jianting Zhou et al.   NUCLEIC ACIDS RESEARCH

Cloning and Heterologous Expression of the Grecocycline Biosynthetic Gene Cluster

(2016) Oksana Bilyk et al.   PLoS One

Genomic charting of ribosomally synthesized natural product chemical space facilitates targeted mining

(2016) Michael A. Skinnider et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces

(2015) He Huang et al.   ACTA BIOCHIMICA ET BIOPHYSICA SINICA

Pseudomycoicidin, a Class II Lantibiotic from Bacillus pseudomycoides

(2015) Shradha Basi-Chipalu et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Highly efficient editing of the actinorhodin polyketide chain length factor gene in Streptomyces coelicolor M145 using CRISPR/Cas9-CodA(sm) combined system

(2015) Hu Zeng et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp. NP10

(2015) Tatjana Ilic-Tomic et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

CRISPR/Cas9 nuclease cleavage combined with Gibson assembly for seamless cloning

(2015) Jia-Wang Wang et al.   BIOTECHNIQUES

Identification and analysis of the bacterial endosymbiont specialized for production of the chemotherapeutic natural product ET-743

(2015) Michael M. Schofield et al.   ENVIRONMENTAL MICROBIOLOGY

Antibiotics and evolution: food for thought

(2015) C. R. Strachan et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Application of iChip to Grow “Uncultivable” Microorganisms and its Impact on Antibiotic Discovery

(2015) Rinzhin T. Sherpa et al.   JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES

A new antibiotic kills pathogens without detectable resistance

(2015) Losee L. Ling et al.   NATURE

An enzyme-coupled biosensor enables (S)-reticuline production in yeast from glucose

(2015) William C DeLoache et al.   Nature Chemical Biology

antiSMASH 3.0—a comprehensive resource for the genome mining of biosynthetic gene clusters

(2015) Tilmann Weber et al.   NUCLEIC ACIDS RESEARCH

Dynamic signal processing by ribozyme-mediated RNA circuits to control gene expression

(2015) Shensi Shen et al.   NUCLEIC ACIDS RESEARCH

Expanding the Chemical Diversity of the Antitumoral Compound Mithramycin by Combinatorial Biosynthesis and Biocatalysis: The Quest for Mithralogs with Improved Therapeutic Window

(2015) Carmen Méndez et al.   PLANTA MEDICA

In Vitro CRISPR/Cas9 System for Efficient Targeted DNA Editing

(2015) Y. Liu et al.   mBio

Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning of large gene clusters

(2015) Wenjun Jiang et al.   Nature Communications

Directed natural product biosynthesis gene cluster capture and expression in the model bacterium Bacillus subtilis

(2015) Yongxin Li et al.   Scientific Reports

Discovery of Clostrubin, an Exceptional Polyphenolic Polyketide Antibiotic from a Strictly Anaerobic Bacterium

(2014) Sacha Pidot et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Systematic Analysis of Biosynthetic Gene Clusters in the Human Microbiome Reveals a Common Family of Antibiotics

(2014) Mohamed S. Donia et al.   CELL

Genome Mining-Directed Activation of a Silent Angucycline Biosynthetic Gene Cluster inStreptomyces chattanoogensis

(2014) Zhenxing Zhou et al.   CHEMBIOCHEM

Mining the Metabiome: Identifying Novel Natural Products from Microbial Communities

(2014) Aleksandr Milshteyn et al.   CHEMISTRY & BIOLOGY

Direct Capture and Heterologous Expression of Salinispora Natural Product Genes for the Biosynthesis of Enterocin

(2014) Bailey Bonet et al.   JOURNAL OF NATURAL PRODUCTS

Principles of genetic circuit design

(2014) Jennifer A N Brophy et al.   NATURE METHODS

Heterologous Expression of the Thiopeptide Antibiotic GE2270 from Planobispora rosea ATCC 53733 in Streptomyces coelicolor Requires Deletion of Ribosomal Genes from the Expression Construct

(2014) Katrin Flinspach et al.   PLoS One

Genomics of Sponge-Associated Streptomyces spp. Closely Related to Streptomyces albus J1074: Insights into Marine Adaptation and Secondary Metabolite Biosynthesis Potential

(2014) Elena Ian et al.   PLoS One

Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A

(2014) Kazuya Yamanaka et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Diversity and evolution of secondary metabolism in the marine actinomycete genus Salinispora

(2014) N. Ziemert et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Targeted Capture and Heterologous Expression of the Pseudoalteromonas Alterochromide Gene Cluster in Escherichia coli Represents a Promising Natural Product Exploratory Platform

(2014) Avena C. Ross et al.   ACS Synthetic Biology

High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System

(2014) Ryan E. Cobb et al.   ACS Synthetic Biology

Activation and identification of five clusters for secondary metabolites inStreptomyces albus J1074

(2014) Carlos Olano et al.   Microbial Biotechnology

A Decade of Riboswitches

(2013) Alexander Serganov et al.   CELL

Seven Ways to Preserve the Miracle of Antibiotics

(2013) J. G. Bartlett et al.   CLINICAL INFECTIOUS DISEASES

Cloning, Characterization and Heterologous Expression of the Indolocarbazole Biosynthetic Gene Cluster from Marine-Derived Streptomyces sanyensis FMA

(2013) Tong Li et al.   Marine Drugs

Molecular Regulation of Antibiotic Biosynthesis in Streptomyces

(2013) G. Liu et al.   MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS

BAGEL3: automated identification of genes encoding bacteriocins and (non-)bactericidal posttranslationally modified peptides

(2013) Auke J. van Heel et al.   NUCLEIC ACIDS RESEARCH

Discovery of indolotryptoline antiproliferative agents by homology-guided metagenomic screening

(2013) F.-Y. Chang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The posttranslational modification cascade to the thiopeptide berninamycin generates linear forms and altered macrocyclic scaffolds

(2013) S. J. Malcolmson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Activation and characterization of a cryptic polycyclic tetramate macrolactam biosynthetic gene cluster

(2013) Yunzi Luo et al.   Nature Communications

Engineered Streptomyces avermitilis Host for Heterologous Expression of Biosynthetic Gene Cluster for Secondary Metabolites

(2013) Mamoru Komatsu et al.   ACS Synthetic Biology

Diversity and isolation of rare actinomycetes: an overview

(2012) Kavita Tiwari et al.   CRITICAL REVIEWS IN MICROBIOLOGY

A Novel Mithramycin Analogue with High Antitumor Activity and Less Toxicity Generated by Combinatorial Biosynthesis

(2012) Luz E. Núñez et al.   JOURNAL OF MEDICINAL CHEMISTRY

Tetarimycin A, an MRSA-Active Antibiotic Identified through Induced Expression of Environmental DNA Gene Clusters

(2012) Dimitris Kallifidas et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Synthetic biology and the development of tools for metabolic engineering

(2012) Jay D. Keasling   METABOLIC ENGINEERING

Are Uncultivated Bacteria Really Uncultivable?

(2012) Indun Dewi Puspita et al.   MICROBES AND ENVIRONMENTS

Genome streamlining and chemical defense in a coral reef symbiosis

(2012) J. C. Kwan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure and biosynthesis of the unusual polyketide alkaloid coelimycin P1, a metabolic product of the cpk gene cluster of Streptomyces coelicolor M145

(2012) Juan Pablo Gomez-Escribano et al.   Chemical Science

Polyketide synthase pathways identified from a metagenomic library are derived from soil Acidobacteria

(2011) Larissa C. Parsley et al.   FEMS MICROBIOLOGY ECOLOGY

Proposals Seek to Reduce Resistance, Boost Development of New Antibiotics

(2011) Bridget M. Kuehn   JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION

Identification of a bioactive 51-membered macrolide complex by activation of a silent polyketide synthase in Streptomyces ambofaciens

(2011) L. Laureti et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genetically Engineered Lipopeptide Antibiotics Related to A54145 and Daptomycin with Improved Properties

(2010) K. T. Nguyen et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

Sequence-Based Analysis of Secondary-Metabolite Biosynthesis in Marine Actinobacteria

(2010) E. A. Gontang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Exploitation of phage battery in the search for bioactive actinomycetes

(2010) D. İpek Kurtböke   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Cloning large natural product gene clusters from the environment: Piecing environmental DNA gene clusters back together with TAR

(2010) Jeffrey H. Kim et al.   BIOPOLYMERS

Heterologous expression of the biosynthetic gene clusters of coumermycin A1, clorobiocin and caprazamycins in genetically modified Streptomyces coelicolor strains

(2010) Katrin Flinspach et al.   BIOPOLYMERS

Deletion of a regulatory gene within the cpk gene cluster reveals novel antibacterial activity in Streptomyces coelicolor A3(2)

(2010) M. Gottelt et al.   MICROBIOLOGY-SGM

Exploiting plug-and-play synthetic biology for drug discovery and production in microorganisms

(2010) Marnix H. Medema et al.   NATURE REVIEWS MICROBIOLOGY

Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism

(2010) M. Komatsu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineering Streptomyces coelicolor for heterologous expression of secondary metabolite gene clusters

(2010) Juan Pablo Gomez-Escribano et al.   Microbial Biotechnology

Production of the Novel Two-Peptide Lantibiotic Lichenicidin by Bacillus licheniformis DSM 13

(2009) Jasmin Dischinger et al.   PLoS One

Improved Antifungal Polyene Macrolides via Engineering of the Nystatin Biosynthetic Genes in Streptomyces noursei

(2008) Trygve Brautaset et al.   CHEMISTRY & BIOLOGY

Mithramycin Analogues Generated by Combinatorial Biosynthesis Show Improved Bioactivity

(2008) Irfan Baig et al.   JOURNAL OF NATURAL PRODUCTS

Non-ribosomal peptide synthetase module fusions to produce derivatives of daptomycin in Streptomyces roseosporus

(2008) S. Doekel et al.   MICROBIOLOGY-SGM