Biosynthetic engineering of nonribosomal peptide synthetases

Targeting Antibiotic Resistance

(2016) Mathieu F. Chellat et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Reprogramming the Biosynthesis of Cyclodepsipeptide Synthetases to Obtain New Enniatins and Beauvericins

(2016) Sophia Zobel et al.   CHEMBIOCHEM

Recent advances in engineering nonribosomal peptide assembly lines

(2016) M. Winn et al.   NATURAL PRODUCT REPORTS

A structural model for multimodular NRPS assembly lines

(2016) Mohamed A. Marahiel   NATURAL PRODUCT REPORTS

Structures of two distinct conformations of holo-non-ribosomal peptide synthetases

(2016) Eric J. Drake et al.   NATURE

A Subdomain Swap Strategy for Reengineering Nonribosomal Peptides

(2015) Hajo Kries et al.   CHEMISTRY & BIOLOGY

A new antibiotic kills pathogens without detectable resistance

(2015) Losee L. Ling et al.   NATURE

Reinvigorating natural product combinatorial biosynthesis with synthetic biology

(2015) Eunji Kim et al.   Nature Chemical Biology

De novo design of a four-fold symmetric TIM-barrel protein with atomic-level accuracy

(2015) Po-Ssu Huang et al.   Nature Chemical Biology

Reprogramming Nonribosomal Peptide Synthetases for “Clickable” Amino Acids

(2014) Hajo Kries et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Genetic manipulation of non-ribosomal peptide synthetases to generate novel bioactive peptide products

(2014) Mark J. Calcott et al.   BIOTECHNOLOGY LETTERS

Advances in the directed evolution of proteins

(2014) Michael D Lane et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Computational tools for designing and engineering enzymes

(2014) Jiri Damborsky et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Accurate design of co-assembling multi-component protein nanomaterials

(2014) Neil P. King et al.   NATURE

Computational design of a red fluorophore ligase for site-specific protein labeling in living cells

(2014) D. S. Liu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Nonproteinogenic Amino Acid Building Blocks for Nonribosomal Peptide and Hybrid Polyketide Scaffolds

(2013) Christopher T. Walsh et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Engineering the Substrate Specificity of the DhbE Adenylation Domain by Yeast Cell Surface Display

(2013) Keya Zhang et al.   CHEMISTRY & BIOLOGY

De novo enzymes by computational design

(2013) Hajo Kries et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Engineering polyketide synthases and nonribosomal peptide synthetases

(2013) Gavin J Williams   CURRENT OPINION IN STRUCTURAL BIOLOGY

Antibiotic resistance—the need for global solutions

(2013) Ramanan Laxminarayan et al.   LANCET INFECTIOUS DISEASES

Precision is essential for efficient catalysis in an evolved Kemp eliminase

(2013) Rebecca Blomberg et al.   NATURE

Evolution of a designed retro-aldolase leads to complete active site remodeling

(2013) Lars Giger et al.   Nature Chemical Biology

Platforms for antibiotic discovery

(2013) Kim Lewis   NATURE REVIEWS DRUG DISCOVERY

Introduction of a Non-Natural Amino Acid into a Nonribosomal Peptide Antibiotic by Modification of Adenylation Domain Specificity

(2012) Jenny Thirlway et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Structural and Functional Investigation of the Intermolecular Interaction between NRPS Adenylation and Carrier Protein Domains

(2012) Jesse A. Sundlov et al.   CHEMISTRY & BIOLOGY

Ribosome-independent biosynthesis of biologically active peptides: Application of synthetic biology to generate structural diversity

(2012) Tobias W. Giessen et al.   FEBS LETTERS

Structural Basis of the Interaction of MbtH-like Proteins, Putative Regulators of Nonribosomal Peptide Biosynthesis, with Adenylating Enzymes

(2012) Dominik A. Herbst et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The generation of “unNatural” products: Synthetic biology meets synthetic chemistry

(2012) Rebecca J. M. Goss et al.   NATURAL PRODUCT REPORTS

Engineering the third wave of biocatalysis

(2012) U. T. Bornscheuer et al.   NATURE

Iterative approach to computational enzyme design

(2012) H. K. Privett et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Evolution-guided engineering of nonribosomal peptide synthetase adenylation domains

(2012) Max Crüsemann et al.   Chemical Science

Combinatorial Biosynthesis of Cyclic Lipopeptide Antibiotics: A Model for Synthetic Biology To Accelerate the Evolution of Secondary Metabolite Biosynthetic Pathways

(2012) Richard H. Baltz   ACS Synthetic Biology

Directed Evolution of a Gatekeeper Domain in Nonribosomal Peptide Synthesis

(2011) Benoit Villiers et al.   CHEMISTRY & BIOLOGY

Tailor-Made Peptide Synthetases

(2011) Hajo Kries et al.   CHEMISTRY & BIOLOGY

Directed Evolution of the Nonribosomal Peptide Synthetase AdmK Generates New Andrimid Derivatives In Vivo

(2011) Bradley S. Evans et al.   CHEMISTRY & BIOLOGY

Insights into the Biosynthesis of Hormaomycin, An Exceptionally Complex Bacterial Signaling Metabolite

(2011) Ivonne Höfer et al.   CHEMISTRY & BIOLOGY

A system for the continuous directed evolution of biomolecules

(2011) Kevin M. Esvelt et al.   NATURE

NRPSpredictor2—a web server for predicting NRPS adenylation domain specificity

(2011) Marc Röttig et al.   NUCLEIC ACIDS RESEARCH

De Novo Enzyme Design Using Rosetta3

(2011) Florian Richter et al.   PLoS One

Biocatalysts by evolution

(2010) Christian Jäckel et al.   CURRENT OPINION IN BIOTECHNOLOGY

Optimizing non-natural protein function with directed evolution

(2010) Eric M Brustad et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Control of active-site compression

(2010) Judith P. Klinman   Nature Chemistry

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

(2010) J. J. Agresti et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

An exciting but challenging road ahead for computational enzyme design

(2010) David Baker   PROTEIN SCIENCE

Computational Design of an Enzyme Catalyst for a Stereoselective Bimolecular Diels-Alder Reaction

(2010) J. B. Siegel et al.   SCIENCE

Mapping the Limits of Substrate Specificity of the Adenylation Domain of TycA

(2009) Benoit R. M. Villiers et al.   CHEMBIOCHEM

Designing artificial enzymes by intuition and computation

(2009) Vikas Nanda et al.   Nature Chemistry

Computational structure-based redesign of enzyme activity

(2009) C.-Y. Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Addressing the Numbers Problem in Directed Evolution

(2008) Manfred T. Reetz et al.   CHEMBIOCHEM

Kemp elimination catalysts by computational enzyme design

(2008) Daniela Röthlisberger et al.   NATURE

De Novo Computational Design of Retro-Aldol Enzymes

(2008) L. Jiang et al.   SCIENCE

Crystal Structure of the Termination Module of a Nonribosomal Peptide Synthetase

(2008) A. Tanovic et al.   SCIENCE