Cloning and Heterologous Expression of the Grecocycline Biosynthetic Gene Cluster

Highly efficient CRISPR/Cas9-mediated TAR cloning of genes and chromosomal loci from complex genomes in yeast

(2015) Nicholas C.O. Lee et al.   NUCLEIC ACIDS RESEARCH

Insights into naturally minimised Streptomyces albus J1074 genome

(2014) Nestor Zaburannyi et al.   BMC GENOMICS

Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A

(2014) Kazuya Yamanaka et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

antiSMASH 2.0—a versatile platform for genome mining of secondary metabolite producers

(2013) Kai Blin et al.   NUCLEIC ACIDS RESEARCH

Delineation of gilvocarcin, jadomycin, and landomycin pathways through combinatorial biosynthetic enzymology

(2012) Madan K Kharel et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Customized optimization of metabolic pathways by combinatorial transcriptional engineering

(2012) Jing Du et al.   NUCLEIC ACIDS RESEARCH

Elucidation of post-PKS tailoring steps involved in landomycin biosynthesis

(2012) Madan K. Kharel et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Tandem assembly of the epothilone biosynthetic gene cluster by in vitro site-specific recombination

(2011) Lin Zhang et al.   Scientific Reports

Cloning large natural product gene clusters from the environment: Piecing environmental DNA gene clusters back together with TAR

(2010) Jeffrey H. Kim et al.   BIOPOLYMERS

Grecocyclines: New Angucyclines from Streptomyces sp. Acta 1362

(2010) Thomas Paululat et al.   EUROPEAN JOURNAL OF ORGANIC CHEMISTRY

Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli

(2010) P. K. Ajikumar et al.   SCIENCE

Cloning and Sequencing of the Biosynthetic Gene Cluster for Saquayamycin Z and Galtamycin B and the Elucidation of the Assembly of Their Saccharide Chains

(2009) Annette Erb et al.   CHEMBIOCHEM

A plasmid RK2-based broad-host-range cloning vector useful for transfer of metagenomic libraries to a variety of bacterial species

(2009) Trine Aakvik et al.   FEMS MICROBIOLOGY LETTERS

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Elucidation of Oxygenation Steps during Oviedomycin Biosynthesis and Generation of Derivatives with Increased Antitumor Activity

(2008) Felipe Lombó et al.   CHEMBIOCHEM

Selective isolation of genomic loci from complex genomes by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae

(2008) Natalay Kouprina et al.   Nature Protocols