Caught green-handed: methods for in vivo detection and visualization of protease activity

Chemogenetic control of gene expression and cell signaling with antiviral drugs

(2018) Elliot P. Tague et al.   NATURE METHODS

Bioorthogonal click chemistry for fluorescence imaging of choline phospholipids in plants

(2018) Janet M. Paper et al.   Plant Methods

Plant life needs cell death, but does plant cell death need Cys proteases?

(2017) Daniela J. Sueldo et al.   FEBS Journal

Ubiquitylation activates a peptidase that promotes cleavage and destabilization of its activating E3 ligases and diverse growth regulatory proteins to limit cell proliferation inArabidopsis

(2017) Hui Dong et al.   GENES & DEVELOPMENT

A molecular signature for anastasis, recovery from the brink of apoptotic cell death

(2017) Gongping Sun et al.   JOURNAL OF CELL BIOLOGY

Green light for quantitative live-cell imaging in plants

(2017) Guido Grossmann et al.   JOURNAL OF CELL SCIENCE

Quantitative proteomics in plant protease substrate identification

(2017) Fatih Demir et al.   NEW PHYTOLOGIST

Activation of the Arabidopsis membrane-bound transcription factor bZIP28 is mediated by site-2 protease, but not site-1 protease

(2017) Yuji Iwata et al.   PLANT JOURNAL

Improved split fluorescent proteins for endogenous protein labeling

(2017) Siyu Feng et al.   Nature Communications

Bright Bioluminescent BRET Sensor Proteins for Measuring Intracellular Caspase Activity

(2017) Anniek den Hamer et al.   ACS Sensors

Development of a luciferase-based biosensor to assess enterovirus 71 3C protease activity in living cells

(2017) Yuan Zhang et al.   Scientific Reports

A FRET-based biosensor for the detection of neutrophil elastase

(2016) C. Schulenburg et al.   ANALYST

Detection of protease activity in cells and animals

(2016) Martijn Verdoes et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Protease substrate profiling using bacterial display of self-blocking affinity proteins and flow-cytometric sorting

(2016) Lisa Sandersjöö et al.   Biotechnology Journal

Functional Characterization of Propeptides in Plant Subtilases as Intramolecular Chaperones and Inhibitors of the Mature Protease

(2016) Michael Meyer et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Juggling jobs: roles and mechanisms of multifunctional protease inhibitors in plants

(2016) Friederike M. Grosse-Holz et al.   NEW PHYTOLOGIST

The Increasing Impact of Activity-Based Protein Profiling in Plant Science

(2016) Kyoko Morimoto et al.   PLANT AND CELL PHYSIOLOGY

A Guide to Fluorescent Protein FRET Pairs

(2016) Bryce Bajar et al.   SENSORS

Versatile protein tagging in cells with split fluorescent protein

(2016) Daichi Kamiyama et al.   Nature Communications

Protease substrate profiling using bacterial display of self-blocking affinity proteins and flow-cytometric sorting

(2016) Lisa Sandersjöö et al.   Biotechnology Journal

CasExpress reveals widespread and diverse patterns of cell survival of caspase-3 activation during development in vivo

(2016) Austin Xun Ding et al.   eLife

Protein Maturation and Proteolysis in Plant Plastids, Mitochondria, and Peroxisomes

(2015) Klaas J. van Wijk   Annual Review of Plant Biology

Protease signaling in animal and plant-regulated cell death

(2015) Guy S. Salvesen et al.   FEBS Journal

Tunable and reversible drug control of protein production via a self-excising degron

(2015) Hokyung K Chung et al.   Nature Chemical Biology

Site-Specific Nitrosoproteomic Identification of EndogenouslyS-Nitrosylated Proteins in Arabidopsis

(2015) Jiliang Hu et al.   PLANT PHYSIOLOGY

Rationally designed fluorogenic protease reporter visualizes spatiotemporal dynamics of apoptosis in vivo

(2015) Tsz-Leung To et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A biosensor for the activity of the “sheddase” TACE (ADAM17) reveals novel and cell type–specific mechanisms of TACE activation

(2015) Douglas A. Chapnick et al.   Science Signaling

In vivo CaspaseTracker biosensor system for detecting anastasis and non-apoptotic caspase activity

(2015) Ho Lam Tang et al.   Scientific Reports

Extracellular peptidase hunting for improvement of protein production in plant cells and roots

(2015) Jérôme Lallemand et al.   Frontiers in Plant Science

Vacuolar processing enzyme in plant programmed cell death

(2015) Noriyuki Hatsugai et al.   Frontiers in Plant Science

A biosensor for the activity of the “sheddase” TACE (ADAM17) reveals novel and cell type–specific mechanisms of TACE activation

(2015) Douglas A. Chapnick et al.   Science Signaling

Activity-Based Profiling of Proteases

(2014) Laura E. Sanman et al.   Annual Review of Biochemistry

Protein Splicing: How Inteins Escape from Precursor Proteins

(2014) Kenneth V. Mills et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The Cysteine Protease CEP1, a Key Executor Involved in Tapetal Programmed Cell Death, Regulates Pollen Development inArabidopsis

(2014) Dandan Zhang et al.   PLANT CELL

Genetically encoded FRET-based biosensor for imaging MMP-9 activity

(2013) Michal Stawarski et al.   BIOMATERIALS

A Tunable, Modular Approach to Fluorescent Protease-Activated Reporters

(2013) Peng Wu et al.   BIOPHYSICAL JOURNAL

Engineering of weak helper interactions for high-efficiency FRET probes

(2013) Raik Grünberg et al.   NATURE METHODS

iGLuc: a luciferase-based inflammasome and protease activity reporter

(2013) Eva Bartok et al.   NATURE METHODS

A Membrane-Bound NAC Transcription Factor, ANAC017, Mediates Mitochondrial Retrograde Signaling in Arabidopsis

(2013) S. Ng et al.   PLANT CELL

Set-point control of RD21 protease activity by AtSerpin1 controls cell death in Arabidopsis

(2013) Nardy Lampl et al.   PLANT JOURNAL

In-Cell Protease Assay Systems Based on Trans-Localizing Molecular Beacon Proteins Using HCV Protease as a Model System

(2013) Jeong Hee Kim et al.   PLoS One

Differential processing of Arabidopsis ubiquitin-like Atg8 autophagy proteins by Atg4 cysteine proteases

(2013) J. Woo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineering fluorescent protein substrates for the AAA+ Lon protease

(2013) M. L. Wohlever et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Visualization of caspase-3-like activity in cells using a genetically encoded fluorescent biosensor activated by protein cleavage

(2013) Jiao Zhang et al.   Nature Communications

Engineered Luciferase Reporter from a Deep Sea Shrimp Utilizing a Novel Imidazopyrazinone Substrate

(2012) Mary P. Hall et al.   ACS Chemical Biology

A Nonpeptidic Cathepsin S Activity-Based Probe for Noninvasive Optical Imaging of Tumor-Associated Macrophages

(2012) Martijn Verdoes et al.   CHEMISTRY & BIOLOGY

Separase Sensor Reveals Dual Roles for Separase Coordinating Cohesin Cleavage and Cdk1 Inhibition

(2012) Norihisa Shindo et al.   DEVELOPMENTAL CELL

Functional Imaging of Legumain in Cancer Using a New Quenched Activity-Based Probe

(2012) Laura E. Edgington et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Cell survival, DNA damage, and oncogenic transformation after a transient and reversible apoptotic response

(2012) Ho Lam Tang et al.   MOLECULAR BIOLOGY OF THE CELL

Activity profiling of vacuolar processing enzymes reveals a role for VPE during oomycete infection

(2012) Johana C. Misas-Villamil et al.   PLANT JOURNAL

The Extended Cleavage Specificity of Human Thrombin

(2012) Maike Gallwitz et al.   PLoS One

Post-Translational Regulation and Trafficking of the Granulin-Containing Protease RD21 of Arabidopsis thaliana

(2012) Christian Gu et al.   PLoS One

Structural basis of fluorescence quenching in caspase activatable-GFP

(2012) Samantha B. Nicholls et al.   PROTEIN SCIENCE

Regulating the reapers: activating metacaspases for programmed cell death

(2012) Eric Lam et al.   TRENDS IN PLANT SCIENCE

Mechanism of a Genetically Encoded Dark-to-Bright Reporter for Caspase Activity

(2011) Samantha B. Nicholls et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Asparagine Peptide Lyases

(2011) Neil David Rawlings et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Substrate Profiling of Tobacco Etch Virus Protease Using a Novel Fluorescence-Assisted Whole-Cell Assay

(2011) George Kostallas et al.   PLoS One

Novel Fluorescence-Assisted Whole-Cell Assay for Engineering and Characterization of Proteases and Their Substrates

(2010) G. Kostallas et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Plant cystatins

(2010) Meriem Benchabane et al.   BIOCHIMIE

Protease Activation of Split Green Fluorescent Protein

(2010) Brian P. Callahan et al.   CHEMBIOCHEM

Activity-based probes: discovering new biology and new drug targets

(2010) William P. Heal et al.   CHEMICAL SOCIETY REVIEWS

Phytaspase, a relocalisable cell death promoting plant protease with caspase specificity

(2010) Nina V Chichkova et al.   EMBO JOURNAL

Proteasome Activity Imaging and Profiling Characterizes Bacterial Effector Syringolin A

(2010) I. Kolodziejek et al.   PLANT PHYSIOLOGY

Copper-free click chemistry in living animals

(2010) P. V. Chang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

TEV protease-facilitated stoichiometric delivery of multiple genes using a single expression vector

(2010) Xi Chen et al.   PROTEIN SCIENCE

Designing Protease Sensors for Real-Time Imaging of Trypsin Activation in Pancreatic Cancer Cells†

(2009) Ning Chen et al.   BIOCHEMISTRY

Rational design of novel red-shifted BRET pairs: Platforms for real-time single-chain protease biosensors

(2009) Seth T. Gammon et al.   BIOTECHNOLOGY PROGRESS

Designing Caspase-3 Sensors for Imaging of Apoptosis in Living Cells

(2009) Ning Chen et al.   CHEMISTRY-A EUROPEAN JOURNAL

An Autoinhibited Coiled-Coil Design Strategy for Split-Protein Protease Sensors

(2009) Sujan S. Shekhawat et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Diversity of Serine Hydrolase Activities of Unchallenged andBotrytis-infectedArabidopsis thaliana

(2009) Farnusch Kaschani et al.   MOLECULAR & CELLULAR PROTEOMICS

Noninvasive optical imaging of apoptosis by caspase-targeted activity-based probes

(2009) Laura E Edgington et al.   NATURE MEDICINE

Real-Time Detection of Caspase-3-Like Protease Activation in Vivo Using Fluorescence Resonance Energy Transfer during Plant Programmed Cell Death Induced by Ultraviolet C Overexposure

(2009) L. Zhang et al.   PLANT PHYSIOLOGY

Novel Genetically Encoded Biosensors Using Firefly Luciferase

(2008) Frank Fan et al.   ACS Chemical Biology

Plant Proteases: From Phenotypes to Molecular Mechanisms

(2008) Renier A. L. van der Hoorn   Annual Review of Plant Biology

Fluorescence Resonance Energy Transfer-Based Assay for Characterization of Hepatitis C Virus NS3-4A Protease Activity in Live Cells

(2008) R. Sabariegos et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

DEVD-NucView488: a novel class of enzyme substrates for real-time detection of caspase-3 activity in live cells

(2008) Hui Cen et al.   FASEB JOURNAL

Visualization of Polarized Membrane Type 1 Matrix Metalloproteinase Activity in Live Cells by Fluorescence Resonance Energy Transfer Imaging

(2008) Mingxing Ouyang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Characterization of a randomized FRET library for protease specificity determination

(2008) Jonathan F. Fretwell et al.   Molecular BioSystems

Minitags for small molecules: detecting targets of reactive small molecules in living plant tissues using ‘click chemistry’

(2008) Farnusch Kaschani et al.   PLANT JOURNAL

Mutational optimization of the coelenterazine-dependent luciferase from Renilla

(2008) Jongchan Woo et al.   Plant Methods

A fluorescent reporter of caspase activity for live imaging

(2008) P.-L. Bardet et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A drug-controllable tag for visualizing newly synthesized proteins in cells and whole animals

(2008) M. Z. Lin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Proteomic analysis of S-nitrosylated proteins inArabidopsis thaliana undergoing hypersensitive response

(2008) Maria C. Romero-Puertas et al.   PROTEOMICS