Non-animal test methods for predicting skin sensitization potentials

An expert consortium review of the EC-commissioned report “Alternative (Non-Animal) Methods for Cosmetics Testing: Current Status and Future Prospects – 2010”

(2014) Thomas Hartung   ALTEX-Alternatives to Animal Experimentation

Local Lymph Node Assay: how testing laboratories apply OECD TG 429 for REACH purposes

(2014) Costanza Rovida   ALTEX-Alternatives to Animal Experimentation

Skin sensitisation: the Colipa strategy for developing and evaluating non-animal test methods for risk assessment

(2014) Gavin Maxwell   ALTEX-Alternatives to Animal Experimentation

A critique of the EC’s expert (draft) reports on the status of alternatives for cosmetics testing to meet the 2013 deadline

(2014) Katy Taylor   ALTEX-Alternatives to Animal Experimentation

Integrating non-animal test information into an adaptive testing strategy – skin sensitization proof of concept case

(2014) Joanna Jaworska   ALTEX-Alternatives to Animal Experimentation

Assessment of the sensitizing potential of textile disperse dyes and some of their metabolites by the loose-fit coculture-based sensitization assay (LCSA)

(2012) Anna Sonnenburg et al.   ARCHIVES OF TOXICOLOGY

Putting the parts together: Combining in vitro methods to test for skin sensitizing potentials

(2012) Caroline Bauch et al.   REGULATORY TOXICOLOGY AND PHARMACOLOGY

Implementation challenges for designing Integrated In Vitro Testing Strategies (ITS) aiming at reducing and replacing animal experimentation

(2012) Bart De Wever et al.   TOXICOLOGY IN VITRO

Transfer of a two-tiered keratinocyte assay: IL-18 production by NCTC2544 to determine the skin sensitizing capacity and epidermal equivalent assay to determine sensitizer potency

(2012) Marc Teunis et al.   TOXICOLOGY IN VITRO

Alternative (non-animal) methods for cosmetics testing: current status and future prospects—2010

(2011) Sarah Adler et al.   ARCHIVES OF TOXICOLOGY

A genomic biomarker signature can predict skin sensitizers using a cell-based in vitro alternative to animal tests

(2011) Henrik Johansson et al.   BMC GENOMICS

Allergic contact dermatitis: epidemiology, molecular mechanisms, in vitro methods and regulatory aspects

(2011) M. Peiser et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Modification and Expulsion of Keratins by Human Epidermal Keratinocytes upon Hapten Exposure in Vitro

(2011) Brigitte Bauer et al.   CHEMICAL RESEARCH IN TOXICOLOGY

Predictive performance for human skin sensitizing potential of the human cell line activation test (h-CLAT)

(2011) Yuko Nukada et al.   CONTACT DERMATITIS

A new organotypic model containing dermal-type macrophages

(2011) Nicolas Bechetoille et al.   EXPERIMENTAL DERMATOLOGY

Evaluation of the skin sensitization potential of chemicals in THP-1/keratinocyte co-cultures

(2011) Yu-ping Cao et al.   IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY

The relationship between CD86 and CD54 protein expression and cytotoxicity following stimulation with contact allergen in THP-1 cells

(2011) Yuko Nukada et al.   JOURNAL OF TOXICOLOGICAL SCIENCES

Evaluating the sensitization potential of surfactants: Integrating data from the local lymph node assay, guinea pig maximization test, and in vitro methods in a weight-of-evidence approach

(2011) Nicholas Ball et al.   REGULATORY TOXICOLOGY AND PHARMACOLOGY

An In Vitro Test to Screen Skin Sensitizers Using a Stable THP-1–Derived IL-8 Reporter Cell Line, THP-G8

(2011) Toshiya Takahashi et al.   TOXICOLOGICAL SCIENCES

The Incorporation of Lysine into the Peroxidase Peptide Reactivity Assay for Skin Sensitization Assessments

(2011) John A. Troutman et al.   TOXICOLOGICAL SCIENCES

Cross Talk between Keratinocytes and Dendritic Cells: Impact on the Prediction of Sensitization

(2011) Jenny Hennen et al.   TOXICOLOGICAL SCIENCES

Inter-laboratory study of the in vitro dendritic cell migration assay for identification of contact allergens

(2011) B. Rees et al.   TOXICOLOGY IN VITRO

Intralaboratory validation of four in vitro assays for the prediction of the skin sensitizing potential of chemicals

(2011) Caroline Bauch et al.   TOXICOLOGY IN VITRO

Functionality and specificity of gene markers for skin sensitization in dendritic cells

(2011) Nathalie Lambrechts et al.   TOXICOLOGY LETTERS

T-cell recognition of chemicals, protein allergens and drugs: towards the development of in vitro assays

(2010) Stefan F. Martin et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Global QSAR models of skin sensitisers for regulatory purposes

(2010) Qasim Chaudhry et al.   Chemistry Central Journal

Non-animal sensitization testing: State-of-the-art

(2010) Rob J. Vandebriel et al.   CRITICAL REVIEWS IN TOXICOLOGY

A newin vitromethod for identifying chemical sensitizers combining peptide binding with ARE/EpRE-mediated gene expression in human skin cells

(2010) James M. McKim et al.   Cutaneous and Ocular Toxicology

Inter-laboratory validation of the modified murine local lymph node assay based on 5-bromo-2′-deoxyuridine incorporation

(2010) Hajime Kojima et al.   JOURNAL OF APPLIED TOXICOLOGY

Assessment of Chemical Skin-Sensitizing Potency by an In Vitro Assay Based on Human Dendritic Cells

(2010) Nathalie Lambrechts et al.   TOXICOLOGICAL SCIENCES

Tracking Human Contact Allergens: From Mass Spectrometric Identification of Peptide-Bound Reactive Small Chemicals to Chemical-Specific Naive Human T-Cell Priming

(2010) Lisa Dietz et al.   TOXICOLOGICAL SCIENCES

Keratinocyte Gene Expression Profiles Discriminate Sensitizing and Irritating Compounds

(2010) Rob J. Vandebriel et al.   TOXICOLOGICAL SCIENCES

Chemical Allergy: Translating Biology into Hazard Characterization

(2010) I. Kimber et al.   TOXICOLOGICAL SCIENCES

Performance of a novel keratinocyte-based reporter cell line to screen skin sensitizers in vitro

(2010) Roger Emter et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Identifying and characterizing chemical skin sensitizers without animal testing: Colipa’s research and method development program

(2010) P. Aeby et al.   TOXICOLOGY IN VITRO

Predicting skin sensitization potential and inter-laboratory reproducibility of a human Cell Line Activation Test (h-CLAT) in the European Cosmetics Association (COLIPA) ring trials

(2010) Hitoshi Sakaguchi et al.   TOXICOLOGY IN VITRO

Use of IL-8 release and p38 MAPK activation in THP-1 cells to identify allergens and to assess their potency in vitro

(2010) M. Mitjans et al.   TOXICOLOGY IN VITRO

In vitro detection of contact allergens: Development of an optimized protocol using human peripheral blood monocyte-derived dendritic cells

(2010) Hendrik Reuter et al.   TOXICOLOGY IN VITRO

A potential in vitro epidermal equivalent assay to determine sensitizer potency

(2010) Guilherme G. dos Santos et al.   TOXICOLOGY IN VITRO

The intra- and inter-laboratory reproducibility and predictivity of the KeratinoSens assay to predict skin sensitizers in vitro: Results of a ring-study in five laboratories

(2010) Natsch Andreas et al.   TOXICOLOGY IN VITRO

Further development of the NCTC 2544 IL-18 assay to identify in vitro contact allergens

(2010) V. Galbiati et al.   TOXICOLOGY IN VITRO

Gene markers in dendritic cells unravel pieces of the skin sensitization puzzle

(2010) Nathalie Lambrechts et al.   TOXICOLOGY LETTERS

Prediction of the contact sensitizing potential of chemicals using analysis of gene expression changes in human THP-1 monocytes

(2010) Joanna Arkusz et al.   TOXICOLOGY LETTERS

Th17/Tc17 infiltration and associated cytokine gene expression in elicitation phase of allergic contact dermatitis

(2009) Y. Zhao et al.   BRITISH JOURNAL OF DERMATOLOGY

Expression of surface markers on the human monocytic leukaemia cell line, THP-1, as indicators for the sensitizing potential of chemicals

(2009) Susun An et al.   CONTACT DERMATITIS

Keratinocytes Function as Accessory Cells for Presentation of Endogenous Antigen Expressed in the Epidermis

(2009) Brian S. Kim et al.   JOURNAL OF INVESTIGATIVE DERMATOLOGY

The Phenion® Full-Thickness Skin Model for Percutaneous Absorption Testing

(2009) K. Ackermann et al.   SKIN PHARMACOLOGY AND PHYSIOLOGY

A Toxicology for the 21st Century—Mapping the Road Ahead

(2009) Thomas Hartung   TOXICOLOGICAL SCIENCES

The Nrf2-Keap1-ARE Toxicity Pathway as a Cellular Sensor for Skin Sensitizers—Functional Relevance and a Hypothesis on Innate Reactions to Skin Sensitizers

(2009) Andreas Natsch   TOXICOLOGICAL SCIENCES

Investigation of Peptide Reactivity of Pro-hapten Skin Sensitizers Using a Peroxidase-Peroxide Oxidation System

(2009) G. Frank Gerberick et al.   TOXICOLOGICAL SCIENCES

Reactivity Profiling: Covalent Modification of Single Nucleophile Peptides for Skin Sensitization Risk Assessment

(2009) Maja Aleksic et al.   TOXICOLOGICAL SCIENCES

THP-1 monocytes but not macrophages as a potential alternative for CD34+ dendritic cells to identify chemical skin sensitizers

(2009) Nathalie Lambrechts et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Comparative DNA microarray analysis of human monocyte derived dendritic cells and MUTZ-3 cells exposed to the moderate skin sensitizer cinnamaldehyde

(2009) François Python et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Progress on the development of human in vitro dendritic cell based assays for assessment of the sensitizing potential of a compound

(2009) G. Galvão dos Santos et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Gene profiles of THP-1 macrophages after in vitro exposure to respiratory (non-)sensitizing chemicals: Identification of discriminating genetic markers and pathway analysis

(2009) Sandra Verstraelen et al.   TOXICOLOGY IN VITRO

Use of IL-18 production in a human keratinocyte cell line to discriminate contact sensitizers from irritants and low molecular weight respiratory allergens

(2009) Emanuela Corsini et al.   TOXICOLOGY IN VITRO

Comparison of a novel CXCL12/CCL5 dependent migration assay with CXCL8 secretion and CD86 expression for distinguishing sensitizers from non-sensitizers using MUTZ-3 Langerhans cells

(2009) Krista Ouwehand et al.   TOXICOLOGY IN VITRO

The relationship between CD86/CD54 expression and THP-1 cell viability in an in vitro skin sensitization test – human cell line activation test (h-CLAT)

(2008) Hitoshi Sakaguchi et al.   CELL BIOLOGY AND TOXICOLOGY

Suitability of macrophage inflammatory protein-1β production by THP-1 cells in differentiating skin sensitizers from irritant chemicals

(2008) Yeon-Mi Lim et al.   CONTACT DERMATITIS

Development of a ModifiedIn vitroSkin Absorption Method to Study the Epidermal/Dermal Disposition of a Contact Allergen in Human Skin

(2008) Ruth U. Pendlington et al.   Cutaneous and Ocular Toxicology

Comparison of the skin sensitizing potential of unsaturated compounds as assessed by the murine local lymph node assay (LLNA) and the guinea pig maximization test (GPMT)

(2008) R. Kreiling et al.   FOOD AND CHEMICAL TOXICOLOGY

Role of TNF-α and extracellular ATP in THP-1 cell activation following allergen exposure

(2008) Masaaki Miyazawa et al.   JOURNAL OF TOXICOLOGICAL SCIENCES

Production of IL-8 in THP-1 cells following contact allergen stimulation via mitogen-activated protein kinase activation or tumor necrosis factor-α production

(2008) Yuko Nukada et al.   JOURNAL OF TOXICOLOGICAL SCIENCES

Filling the Concept with Data: Integrating Data from Different In Vitro and In Silico Assays on Skin Sensitizers to Explore the Battery Approach for Animal-Free Skin Sensitization Testing

(2008) Andreas Natsch et al.   TOXICOLOGICAL SCIENCES

LC-MS–Based Characterization of the Peptide Reactivity of Chemicals to Improve the In Vitro Prediction of the Skin Sensitization Potential

(2008) Andreas Natsch et al.   TOXICOLOGICAL SCIENCES

HMOX1 and NQO1 Genes are Upregulated in Response to Contact Sensitizers in Dendritic Cells and THP-1 Cell Line: Role of the Keap1/Nrf2 Pathway

(2008) Nadège Ade et al.   TOXICOLOGICAL SCIENCES

A new dendritic cell type suitable as sentinel of contact allergens

(2008) Maximilian Schreiner et al.   TOXICOLOGY

A cell-based in vitro alternative to identify skin sensitizers by gene expression

(2008) Jef Hooyberghs et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Characterization of early events involved in human dendritic cell maturation induced by sensitizers: Cross talk between MAPK signalling pathways

(2008) Sandra Trompezinski et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Gene expression profiling of in vitro cultured macrophages after exposure to the respiratory sensitizer hexamethylene diisocyanate

(2008) S. Verstraelen et al.   TOXICOLOGY IN VITRO

Reconstruction of three-dimensional human skin model composed of dendritic cells, keratinocytes and fibroblasts utilizing a handy scaffold of collagen vitrigel membrane

(2008) T. Uchino et al.   TOXICOLOGY IN VITRO

Gene profiles of a human alveolar epithelial cell line after in vitro exposure to respiratory (non-)sensitizing chemicals: Identification of discriminating genetic markers and pathway analysis

(2008) Sandra Verstraelen et al.   TOXICOLOGY LETTERS

A review of critical factors in the conduct and interpretation of the human repeat insult patch test

(2007) Pauline M. McNamee et al.   REGULATORY TOXICOLOGY AND PHARMACOLOGY

Role of p38 MAPK in the selective release of IL-8 induced by chemical allergen in naïve THP-1 cells

(2007) Montserrat Mitjans et al.   TOXICOLOGY IN VITRO