EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes intoSaccharomyces cerevisiaevia CRISPR-Cas9

CRISPR/Cas9 advances engineering of microbial cell factories

(2016) Tadas Jakočiūnas et al.   METABOLIC ENGINEERING

The metabolic background is a global player in Saccharomyces gene expression epistasis

(2016) Mohammad Tauqeer Alam et al.   Nature Microbiology

CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae

(2015) Robert Mans et al.   FEMS YEAST RESEARCH

EasyClone 2.0: expanded toolkit of integrative vectors for stable gene expression in industrial Saccharomyces cerevisiae strains

(2015) Vratislav Stovicek et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Establishing a synthetic pathway for high-level production of 3-hydroxypropionic acid in Saccharomyces cerevisiae via β-alanine

(2015) Irina Borodina et al.   METABOLIC ENGINEERING

Multiplex metabolic pathway engineering using CRISPR/Cas9 in Saccharomyces cerevisiae

(2015) Tadas Jakočiūnas et al.   METABOLIC ENGINEERING

New vectors for simple and streamlined CRISPR-Cas9 genome editing inSaccharomyces cerevisiae

(2015) Marian F. Laughery et al.   YEAST

CasEMBLR: Cas9-Facilitated Multiloci Genomic Integration of in Vivo Assembled DNA Parts in Saccharomyces cerevisiae

(2015) Tadas Jakočiu̅nas et al.   ACS Synthetic Biology

Efficient Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas

(2015) Andrew A. Horwitz et al.   Cell Systems

Advances in metabolic engineering of yeastSaccharomyces cerevisiaefor production of chemicals

(2014) Irina Borodina et al.   Biotechnology Journal

Efficient simultaneous excision of multiple selectable marker cassettes using I-SceI-induced double-strand DNA breaks inSaccharomyces cerevisiae

(2014) Daniel Solis-Escalante et al.   FEMS YEAST RESEARCH

Application of synthetic biology for production of chemicals in yeastSaccharomyces cerevisiae

(2014) Mingji Li et al.   FEMS YEAST RESEARCH

Recent applications of synthetic biology tools for yeast metabolic engineering

(2014) Michael K. Jensen et al.   FEMS YEAST RESEARCH

Coupled incremental precursor and co-factor supply improves 3-hydroxypropionic acid production in Saccharomyces cerevisiae

(2014) Yun Chen et al.   METABOLIC ENGINEERING

Engineering Acetyl Coenzyme A Supply: Functional Expression of a Bacterial Pyruvate Dehydrogenase Complex in the Cytosol of Saccharomyces cerevisiae

(2014) B. U. Kozak et al.   mBio

One-step assembly and targeted integration of multigene constructs assisted by the I-SceI meganuclease inSaccharomyces cerevisiae

(2013) Niels G.A. Kuijpers et al.   FEMS YEAST RESEARCH

EasyClone: method for iterative chromosomal integration of multiple genes inSaccharomyces cerevisiae

(2013) Niels B. Jensen et al.   FEMS YEAST RESEARCH

A versatile, efficient strategy for assembly of multi-fragment expression vectors in Saccharomyces cerevisiae using 60 bp synthetic recombination sequences

(2013) Niels GA Kuijpers et al.   Microbial Cell Factories

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

(2013) James E. DiCarlo et al.   NUCLEIC ACIDS RESEARCH

Microbial production of indolylglucosinolate through engineering of a multi-gene pathway in a versatile yeast expression platform

(2012) Michael Dalgaard Mikkelsen et al.   METABOLIC ENGINEERING

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Reiterative Recombination for the in vivo assembly of libraries of multigene pathways

(2011) L. M. Wingler et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA