Random mutagenesis and precise gene editing technologies: applications in algal crop improvement and functional genomics

Construction of Plastid Expression Vector and Development of Genetic Transformation System for the Seaweed Pyropia yezoensis

(2017) Fanna Kong et al.   MARINE BIOTECHNOLOGY

RNAi-based targeted gene knockdown in the model oleaginous microalgae Nannochloropsis oceanica

(2017) Li Wei et al.   PLANT JOURNAL

Evaluation of phenotype stability and ecological risk of a genetically engineered alga in open pond production

(2017) Shawn J. Szyjka et al.   Algal Research-Biomass Biofuels and Bioproducts

Towards developing algal synthetic biology

(2016) M. A. Scaife et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Microalgae engineering toolbox: Selectable and screenable markers

(2016) Khawaja Muhammad Imran Bashir et al.   BIOTECHNOLOGY AND BIOPROCESS ENGINEERING

Improvement of Nannochloropsis oceanica growth performance through chemical mutation and characterization of fast growth physiology by transcriptome profiling

(2016) Sijie Liang et al.   CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY

Algal Cell Factories: Approaches, Applications, and Potentials

(2016) Weiqi Fu et al.   Marine Drugs

CRISPR: gene editing is just the beginning

(2016) Heidi Ledford   NATURE

Protoplast fusion and genetic recombination betweenOchromonas danica(Chrysophyta) andHaematococcus pluvialis(Chlorophyta)

(2016) Abd El-Fatah Abomohra et al.   PHYCOLOGIA

AnAgrobacterium-delivered CRISPR/Cas9 system for high-frequency targeted mutagenesis in maize

(2016) Si Nian Char et al.   PLANT BIOTECHNOLOGY JOURNAL

Genome editing of model oleaginous microalgaeNannochloropsisspp. by CRISPR/Cas9

(2016) Qintao Wang et al.   PLANT JOURNAL

Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana

(2016) Amanda Hopes et al.   Plant Methods

Synergistic Action of D-Glucose and Acetosyringone on Agrobacterium Strains for Efficient Dunaliella Transformation

(2016) Ramachandran Srinivasan et al.   PLoS One

A CRISPR/Cas9 system adapted for gene editing in marine algae

(2016) Marianne Nymark et al.   Scientific Reports

Transgene Expression in Microalgae—From Tools to Applications

(2016) Lior Doron et al.   Frontiers in Plant Science

Transformation of lacZ using different promoters in the commercially important red alga, Gracilaria gracilis

(2016) M Huddy Suzanne et al.   AFRICAN JOURNAL OF BIOTECHNOLOGY

Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing

(2016) Yuichiro Miyaoka et al.   Scientific Reports

CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii

(2016) Sung-Eun Shin et al.   Scientific Reports

CRISPR RNA-guided FokI nucleases repair a PAH variant in a phenylketonuria model

(2016) Yi Pan et al.   Scientific Reports

Improving Cell Growth and Lipid Accumulation in Green Microalgae Chlorella sp. via UV Irradiation

(2015) Shuyu Liu et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

CRISPR-ERA: a comprehensive design tool for CRISPR-mediated gene editing, repression and activation: Fig. 1.

(2015) Honglei Liu et al.   BIOINFORMATICS

Improving high carbon dioxide tolerance and carbon dioxide fixation capability of Chlorella sp. by adaptive laboratory evolution

(2015) Dengjin Li et al.   BIORESOURCE TECHNOLOGY

Construction of a self-cloning system in the unicellular green alga Pseudochoricystis ellipsoidea

(2015) Yuki Kasai et al.   Biotechnology for Biofuels

An integrative Raman microscopy-based workflow for rapid in situ analysis of microalgal lipid bodies

(2015) Sudhir Kumar Sharma et al.   Biotechnology for Biofuels

Targeted genome modifications in soybean with CRISPR/Cas9

(2015) Thomas B Jacobs et al.   BMC BIOTECHNOLOGY

Small Molecules Enhance CRISPR Genome Editing in Pluripotent Stem Cells

(2015) Chen Yu et al.   Cell Stem Cell

Alga-Produced Malaria Transmission-Blocking Vaccine Candidate Pfs25 Formulated with a Human Use-Compatible Potent Adjuvant Induces High-Affinity Antibodies That Block Plasmodium falciparum Infection of Mosquitoes

(2015) Kailash P. Patra et al.   INFECTION AND IMMUNITY

Photo-Oxidative Stress-Driven Mutagenesis and Adaptive Evolution on the Marine Diatom Phaeodactylum tricornutum for Enhanced Carotenoid Accumulation

(2015) Zhiqian Yi et al.   Marine Drugs

Genomic analysis of parallel-evolved cyanobacterium Synechocystis sp. PCC 6803 under acid stress

(2015) Junji Uchiyama et al.   PHOTOSYNTHESIS RESEARCH

Molecular techniques to interrogate and edit theChlamydomonasnuclear genome

(2015) Robert E. Jinkerson et al.   PLANT JOURNAL

Production of genetically and developmentally modified seaweeds: exploiting the potential of artificial selection techniques

(2015) Bénédicte Charrier et al.   Frontiers in Plant Science

Development and Characterization of Somatic Hybrids of Ulva reticulata Forsskål (×) Monostroma oxyspermum (Kutz.)Doty

(2015) Vishal Gupta et al.   Frontiers in Plant Science

Genome editing for crop improvement: Challenges and opportunities

(2015) Naglaa A Abdallah et al.   GM Crops & Food-Biotechnology in Agriculture and the Food Chain

Genetic engineering of the green alga Chlorella zofingiensis: a modified norflurazon-resistant phytoene desaturase gene as a dominant selectable marker

(2014) Jin Liu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases

(2014) S. Bae et al.   BIOINFORMATICS

Development of microalga Scenedesmus dimorphus mutant with higher lipid content by radiation breeding

(2014) Jong-il Choi et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Screening and genetic characterization of thermo-tolerant Synechocystis sp. PCC6803 strains created by adaptive evolution

(2014) Ulrich M Tillich et al.   BMC BIOTECHNOLOGY

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

(2014) Hui-Li Xing et al.   BMC PLANT BIOLOGY

Successful Transient Expression of Cas9 and Single Guide RNA Genes in Chlamydomonas reinhardtii

(2014) Wenzhi Jiang et al.   EUKARYOTIC CELL

Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA

(2014) S. Ramakrishna et al.   GENOME RESEARCH

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

(2014) S. Kim et al.   GENOME RESEARCH

Enhancement of lipid productivity by ethyl methane sulfonate-mediated random mutagenesis and proteomic analysis in Chlamydomonas reinhardtii

(2014) Bongsoo Lee et al.   KOREAN JOURNAL OF CHEMICAL ENGINEERING

Tipping Points in Seaweed Genetic Engineering: Scaling Up Opportunities in the Next Decade

(2014) Hanzhi Lin et al.   Marine Drugs

Metabolomic basis of laboratory evolution of butanol tolerance in photosynthetic Synechocystis sp. PCC 6803

(2014) Yaxing Wang et al.   Microbial Cell Factories

CRISPR-P: A Web Tool for Synthetic Single-Guide RNA Design of CRISPR-System in Plants

(2014) Yang Lei et al.   Molecular Plant

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9

(2014) Samuel H. Sternberg et al.   NATURE

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification

(2014) John P Guilinger et al.   NATURE BIOTECHNOLOGY

Realizing the potential of synthetic biology

(2014) George M. Church et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Inactivation of Phaeodactylum tricornutum urease gene using transcription activator-like effector nuclease-based targeted mutagenesis

(2014) Philip D. Weyman et al.   PLANT BIOTECHNOLOGY JOURNAL

Efficient gene targeting and removal of foreign DNA by homologous recombination in the picoeukaryoteOstreococcus

(2014) Jean-Claude Lozano et al.   PLANT JOURNAL

Artificial microRNA mediated gene silencing in plants: progress and perspectives

(2014) Manish Tiwari et al.   PLANT MOLECULAR BIOLOGY

Using Natural Selection to Explore the Adaptive Potential of Chlamydomonas reinhardtii

(2014) Marie-Mathilde Perrineau et al.   PLoS One

sgRNAcas9: A Software Package for Designing CRISPR sgRNA and Evaluating Potential Off-Target Cleavage Sites

(2014) Shengsong Xie et al.   PLoS One

Development of a Nuclear Transformation System for Oleaginous Green Alga Lobosphaera (Parietochloris) incisa and Genetic Complementation of a Mutant Strain, Deficient in Arachidonic Acid Biosynthesis

(2014) Boris Zorin et al.   PLoS One

A Simple and Non-Invasive Method for Nuclear Transformation of Intact-walled Chlamydomonas reinhardtii

(2014) Sora Kim et al.   PLoS One

Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology

(2014) Fayza Daboussi et al.   Nature Communications

Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system

(2014) Tetsushi Sakuma et al.   Scientific Reports

A technical breakthrough close at hand: feasible approaches toward establishing a gene-targeting genetic transformation system in seaweeds

(2014) Koji Mikami   Frontiers in Plant Science

COSMID: A Web-based Tool for Identifying and Validating CRISPR/Cas Off-target Sites

(2014) Thomas J Cradick et al.   Molecular Therapy-Nucleic Acids

Preparation of transgenic Dunaliella salina for immunization against white spot syndrome virus in crayfish

(2013) Shuying Feng et al.   ARCHIVES OF VIROLOGY

TALENoffer: genome-wide TALEN off-target prediction

(2013) Jan Grau et al.   BIOINFORMATICS

Enhancement of lipid production in low-starch mutants Chlamydomonas reinhardtii by adaptive laboratory evolution

(2013) Shuiyan Yu et al.   BIORESOURCE TECHNOLOGY

Effect of the expression and knockdown of citrate synthase gene on carbon flux during triacylglycerol biosynthesis by green algae Chlamydomonas reinhardtii

(2013) Xiaodong Deng et al.   BMC BIOCHEMISTRY

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases

(2013) S. W. Cho et al.   GENOME RESEARCH

BBS mutations modify phenotypic expression of CEP290-related ciliopathies

(2013) Yan Zhang et al.   HUMAN MOLECULAR GENETICS

Traditional and Modern Plant Breeding Methods with Examples in Rice (Oryza sativa L.)

(2013) Flavio Breseghello et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

A Codon-Optimized Bacterial Antibiotic Gene Used as Selection Marker for Stable Nuclear Transformation in the Marine Red Alga Pyropia yezoensis

(2013) Toshiki Uji et al.   MARINE BIOTECHNOLOGY

Adaptive laboratory evolution – principles and applications for biotechnology

(2013) Martin Dragosits et al.   Microbial Cell Factories

An Efficient Protocol for the Agrobacterium-mediated Genetic Transformation of Microalga Chlamydomonas reinhardtii

(2013) P. T. Pratheesh et al.   MOLECULAR BIOTECHNOLOGY

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

(2013) Seung Woo Cho et al.   NATURE BIOTECHNOLOGY

Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9

(2013) Jian-Feng Li et al.   NATURE BIOTECHNOLOGY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

RNA interference in the nucleus: roles for small RNAs in transcription, epigenetics and beyond

(2013) Stephane E. Castel et al.   NATURE REVIEWS GENETICS

E-TALEN: a web tool to design TALENs for genome engineering

(2013) Florian Heigwer et al.   NUCLEIC ACIDS RESEARCH

Mammalian Base Excision Repair: the Forgotten Archangel

(2013) Grigory L. Dianov et al.   NUCLEIC ACIDS RESEARCH

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Genetic engineering of multispecies microbial cell factories as an alternative for bioenergy production

(2013) Juan Cesar Federico Ortiz-Marquez et al.   TRENDS IN BIOTECHNOLOGY

Cultivated microalgae spills: hard to predict/easier to mitigate risks

(2013) Jonathan Gressel et al.   TRENDS IN BIOTECHNOLOGY

Environmental risks of large scale cultivation of microalgae: Mitigation of spills

(2013) Jonathan Gressel et al.   Algal Research-Biomass Biofuels and Bioproducts

Enhancement of carotenoid biosynthesis in the green microalga Dunaliella salina with light-emitting diodes and adaptive laboratory evolution

(2012) Weiqi Fu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Advances in genetic engineering of marine algae

(2012) Song Qin et al.   BIOTECHNOLOGY ADVANCES

Establishment of an efficient genetic transformation system in Scenedesmus obliquus

(2012) Suo-Lian Guo et al.   JOURNAL OF BIOTECHNOLOGY

Efficient Heterologous Transformation of Chlamydomonas reinhardtii npq2 Mutant with the Zeaxanthin Epoxidase Gene Isolated and Characterized from Chlorella zofingiensis

(2012) Inmaculada Couso et al.   Marine Drugs

Development of a New Method for Genetic Transformation of the Green Alga Chlorella ellipsoidea

(2012) Lili Liu et al.   MOLECULAR BIOTECHNOLOGY

Exploiting diversity and synthetic biology for the production of algal biofuels

(2012) D. Ryan Georgianna et al.   NATURE

TALENs: a widely applicable technology for targeted genome editing

(2012) J. Keith Joung et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Nuclear gene targeting inChlamydomonasusing engineered zinc-finger nucleases

(2012) Irina Sizova et al.   PLANT JOURNAL

The Molecular Diversity of Adaptive Convergence

(2012) O. Tenaillon et al.   SCIENCE

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Initial risk assessment of genetically modified (GM) microalgae for commodity-scale biofuel cultivation

(2012) William J. Henley et al.   Algal Research-Biomass Biofuels and Bioproducts

Application of synthetic biology in cyanobacteria and algae

(2012) Bo Wang et al.   Frontiers in Microbiology

Identification and characterization of microRNAs from Phaeodactylum tricornutum by high-throughput sequencing and bioinformatics analysis

(2011) Aiyou Huang et al.   BMC GENOMICS

The Many Faces of RNAi

(2011) René F. Ketting   DEVELOPMENTAL CELL

RNA-Mediated Silencing in Algae: Biological Roles and Tools for Analysis of Gene Function

(2011) Heriberto Cerutti et al.   EUKARYOTIC CELL

Establishment ofAgrobacterium tumefaciens-mediated genetic transformation inDunaliella bardawil

(2011) N. Anila et al.   EUROPEAN JOURNAL OF PHYCOLOGY

Genome Engineering With Zinc-Finger Nucleases

(2011) Dana Carroll   GENETICS

A new inducible expression system in a transformed green alga, Chlorella vulgaris

(2011) Y.F. Niu et al.   GENETICS AND MOLECULAR RESEARCH

Development of an expression system using the heat shock protein 70 promoter in the red macroalga, Porphyra tenera

(2011) Su Hyun Son et al.   JOURNAL OF APPLIED PHYCOLOGY

Cinnamic acid, coumarin and vanillin: Alternative phenolic compounds for efficient Agrobacterium-mediated transformation of the unicellular green alga, Nannochloropsis sp.

(2011) Thye-San Cha et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Transient Gene Expression System Established in Porphyra yezoensis Is Widely Applicable in Bangiophycean Algae

(2011) Ryo Hirata et al.   MARINE BIOTECHNOLOGY

Enhancement of Lutein Production in Chlorella sorokiniana (Chorophyta) by Improvement of Culture Conditions and Random Mutagenesis

(2011) Baldo F. Cordero et al.   Marine Drugs

Agrobacterium tumefaciens mediated transformation of marine microalgae Schizochytrium

(2011) Rubin Cheng et al.   MICROBIOLOGICAL RESEARCH

Genome-wide analysis of mutagenesis bias and context sensitivity of N-methyl-N′-nitro-N-nitrosoguanidine (NTG)

(2011) Marc Harper et al.   MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS

Stable nuclear transformation of the diatom Chaetoceros sp.

(2011) Arisa Miyagawa-Yamaguchi et al.   PHYCOLOGICAL RESEARCH

The chloroplast transformation toolbox: selectable markers and marker removal

(2011) Anil Day et al.   PLANT BIOTECHNOLOGY JOURNAL

Identification of the UMP Synthase Gene by Establishment of Uracil Auxotrophic Mutants and the Phenotypic Complementation System in the Marine Diatom Phaeodactylum tricornutum

(2011) T. Sakaguchi et al.   PLANT PHYSIOLOGY

A Modular Cloning System for Standardized Assembly of Multigene Constructs

(2011) Ernst Weber et al.   PLoS One

Genetic transformation in Kappaphycus alvarezii using micro-particle bombardment: a potential strategy for germplasm improvement

(2010) Jinfeng Wang et al.   AQUACULTURE INTERNATIONAL

An inducible artificial microRNA system for Chlamydomonas reinhardtii confirms a key role for heat shock factor 1 in regulating thermotolerance

(2010) Stefan Schmollinger et al.   CURRENT GENETICS

Genetic Engineering of Algae for Enhanced Biofuel Production

(2010) Randor Radakovits et al.   EUKARYOTIC CELL

Genetic engineering of fatty acid chain length in Phaeodactylum tricornutum

(2010) Randor Radakovits et al.   METABOLIC ENGINEERING

The Ectocarpus genome and the independent evolution of multicellularity in brown algae

(2010) J. Mark Cock et al.   NATURE

Genome editing with engineered zinc finger nucleases

(2010) Fyodor D. Urnov et al.   NATURE REVIEWS GENETICS

Gene transfer into conchospores of Porphyra haitanensis (Bangiales, Rhodophyta) by glass bead agitation

(2010) Jinfeng Wang et al.   PHYCOLOGIA

Production of therapeutic proteins in algae, analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii

(2010) Beth A. Rasala et al.   PLANT BIOTECHNOLOGY JOURNAL

Identification of miRNA from Porphyra yezoensis by High-Throughput Sequencing and Bioinformatics Analysis

(2010) Chengwei Liang et al.   PLoS One

Factors effecting expression of vaccines in microalgae

(2009) Raymond Surzycki et al.   BIOLOGICALS

Stable nuclear transformation of Gonium pectorale

(2009) Kai Lerche et al.   BMC BIOTECHNOLOGY

Accumulation and processing of a recombinant protein designed as a cleavable fusion to the endogenous Rubisco LSU protein in Chlamydomonas chloroplast

(2009) Machiko Muto et al.   BMC BIOTECHNOLOGY

Origins and Mechanisms of miRNAs and siRNAs

(2009) Richard W. Carthew et al.   CELL

Characterization of the Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Gene from the Halotolerant Alga Dunaliella salina and Inhibition of Its Expression by RNAi

(2009) Yanlong Jia et al.   CURRENT MICROBIOLOGY

Metabolic engineering of cyanobacteria for ethanol production

(2009) Jason Dexter et al.   Energy & Environmental Science

Isolation and characterization of the rbcS genes from a sterile mutant of Ulva pertusa (Ulvales, Chlorophyta) and transient gene expression using the rbcS gene promoter

(2009) Makoto Kakinuma et al.   FISHERIES SCIENCE

Heat-stable oral alga-based vaccine protects mice from Staphylococcus aureus infection

(2009) Imke A.J. Dreesen et al.   JOURNAL OF BIOTECHNOLOGY

AGROBACTERIUM-MEDIATED TRANSFORMATION IN THE GREEN ALGAHAEMATOCOCCUS PLUVIALIS(CHLOROPHYCEAE, VOLVOCALES)

(2009) S. Kathiresan et al.   JOURNAL OF PHYCOLOGY

Visualization of Nuclear Localization of Transcription Factors with Cyan and Green Fluorescent Proteins in the Red Alga Porphyra yezoensis

(2009) Toshiki Uji et al.   MARINE BIOTECHNOLOGY

Visualization of Phosphoinositides via the Development of the Transient Expression System of a Cyan Fluorescent Protein in the Red Alga Porphyra yezoensis

(2009) Koji Mikami et al.   MARINE BIOTECHNOLOGY

Small silencing RNAs: an expanding universe

(2009) Megha Ghildiyal et al.   NATURE REVIEWS GENETICS

Gene silencing in the marine diatom Phaeodactylum tricornutum

(2009) Valentina De Riso et al.   NUCLEIC ACIDS RESEARCH

Enhancement of carotenoids by mutation and stress induced carotenogenic genes in Haematococcus pluvialis mutants

(2008) B. Sandesh Kamath et al.   BIORESOURCE TECHNOLOGY

Unstable RNAi Effects Through Epigenetic Silencing of an Inverted Repeat Transgene in Chlamydomonas reinhardtii

(2008) T. Yamasaki et al.   GENETICS

The integrative expression of GUS gene driven by FCP promoter in the seaweed Laminaria japonica (Phaeophyta)

(2008) Fuchao Li et al.   JOURNAL OF APPLIED PHYCOLOGY

The Pathway via D-Galacturonate/L-Galactonate Is Significant for Ascorbate Biosynthesis inEuglena gracilis

(2008) Takahiro Ishikawa et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Genetically modified plants and human health

(2008) Suzie Key et al.   JOURNAL OF THE ROYAL SOCIETY OF MEDICINE

Inhibition of pds Gene Expression via the RNA Interference Approach in Dunaliella salina (Chlorophyta)

(2008) Guohua Sun et al.   MARINE BIOTECHNOLOGY

Improvement of efficiency of genetic transformation for Dunaliella salina by glass beads method

(2008) Shuying Feng et al.   MOLECULAR BIOLOGY REPORTS

Gene silencing by artificial microRNAs in Chlamydomonas

(2008) Tao Zhao et al.   PLANT JOURNAL

Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii

(2008) Attila Molnar et al.   PLANT JOURNAL

Factors influencing efficiency of transient gene expression in the red macrophyte Porphyra yezoensis

(2008) Satoru Fukuda et al.   PLANT SCIENCE

Recombinant expression of rt-PA gene (encoding Reteplase) in gametophytes of the seaweed Laminaria japonica (Laminariales, Phaeophyta)

(2008) YiChen Zhang et al.   Science in China. Series C, Life sciences / Chinese Academy of Sciences

Polyethylene Glycol (PEG)-Mediated Transient Gene Expression in a Red Alga, Cyanidioschyzon merolae 10D

(2007) M. Ohnuma et al.   PLANT AND CELL PHYSIOLOGY