Article
Biotechnology & Applied Microbiology
Kateryna Kukil, Elias Englund, Nick Crang, Elton P. Hudson, Pia Lindberg
Summary: In this study, phenylalanine resistant mutant strains of the unicellular cyanobacterium Synechocystis sp. PCC 6803 were generated by laboratory evolution. The mutant strains were able to secrete phenylalanine and showed potential for production of trans-cinnamic acid and para-coumaric acid. The PRM8 strain demonstrated the highest specific production of these compounds.
METABOLIC ENGINEERING
(2023)
Article
Genetics & Heredity
Chen Xu, Bing Wang, Lin Yang, Lucas Zhongming Hu, Lanxing Yi, Yaxuan Wang, Shenglan Chen, Andrew Emili, Cuihong Wan
Summary: In this study, using co-fractionation coupled with LC-MS/MS, the researchers identified 291 multi-protein complexes and 24,092 protein-protein interactions in Synechocystis sp. PCC 6803. These interactions reveal the roles of photosynthesis in various physiological processes and highlight the differences in protein functions between bacteria and higher plants.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2022)
Article
Biology
Kaisei Maeda, Yukiko Okuda, Gen Enomoto, Satoru Watanabe, Masahiko Ikeuchi
Summary: Extracellular polysaccharides of bacteria play crucial roles in biofilm formation, stress tolerance, and infectivity, with cyanobacteria uniquely producing sulfated polysaccharides to support phototrophic biofilms. Little is known about the biosynthesis and function of these polysaccharides in cyanobacteria, but a study on Synechocystis sp. strain PCC 6803 has identified the genes responsible for the synthesis of a sulfated polysaccharide called synechan, potentially leading to insights into the functions and biotechnological applications of similar polysaccharides found in cyanobacteria genomes.
Article
Biochemistry & Molecular Biology
Masakazu Toyoshima, Chiaki Yamamoto, Yoshifumi Ueno, Yoshihiro Toya, Seiji Akimoto, Hiroshi Shimizu
Summary: The study revealed that under specific Red1 light conditions, NDH-1 plays a significant role in the growth of Synechocystis, with its main function being in the electron transport pathway it depends on.
Article
Biochemical Research Methods
Ian Sofian Yunus, Zhixuan Wang, Pachara Sattayawat, Jonathan Muller, Fessehaye W. Zemichael, Klaus Hellgardt, Patrik R. Jones
Summary: The study focused on improving the productivity of 1-octanol by enhancing enzyme efficiency and optimizing environmental conditions. By adjusting the inducer concentration and light intensity, the researchers were able to increase the 1-octanol productivity significantly, reaching a carbon partitioning rate of 35% and a titer of 526 mg/L within 12 days.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Microbiology
Jin Ding, Nils Schuergers, Heike Baehre, Annegret Wilde
Summary: This study discovered functional CARF-domain effector and ring nuclease proteins in the cyanobacterium Synechocystis sp. PCC 6803. The Csx1 homolog, SyCsx1, is a strict cytosine-specific RNase that depends on cyclic tetraadenylate (cA4). Another CARF-domain protein similar to Csm6 effectors, SyCsm6, does not have RNase activity but can degrade cOAs and attenuate SyCsx1 RNase activity. This research suggests that the CRISPR systems in Synechocystis have a multi-layered cA4-mediated defense mechanism.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Plant Sciences
Parveen Akhtar, Avratanu Biswas, Fanny Balog-Vig, Ildiko Domonkos, Laszlo Kovacs, Petar H. Lambrev
Summary: Cyanobacterial mutants with monomeric PSI show changes in the composition and abundance of phycobilisomes and in the excitation energy transfer to PSII and PSI. The oligomeric state of PSI impacts the excitation energy flow in Synechocystis.
Article
Plant Sciences
Xiang Li, Chunling Xue, Hui Chen, Huafeng Zhang, Qiang Wang
Summary: This study discovered a small antisense RNA gene (ThfR) that interacts with the thf1 gene, regulating photosynthesis under nitrogen starvation or high-light stress, and enhancing the growth ability of cyanobacteria under high-light stress.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Lucas Gewehr, Benedikt Junglas, Ruven Jilly, Johannes Franz, Wenyu Eva Zhu, Tobias Weidner, Mischa Bonn, Carsten Sachse, Dirk Schneider
Summary: The dynamin-like protein SynDLP in the cyanobacterium Synechocystis sp. PCC 6803 forms ordered oligomers and interacts with negatively charged thylakoid membrane lipids independently of nucleotides. The structural characteristics of SynDLP suggest it to be a bacterial ancestor of eukaryotic dynamin.
NATURE COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Eleftheria-Angeliki Valsami, Angeliki Pateraki, Anastasios Melis, Demetrios F. Ghanotakis
Summary: This study investigated various cultivation conditions for heterologous beta-phellandrene production by Synechocystis sp. PCC 6803, finding that immobilized cells in calcium alginate beads resulted in higher production compared to suspended cultures. Additionally, extreme conditions of pH 10.5 and 600 mM NaCl proved to be conducive for cell growth and beta-phellandrene production, offering new approaches for sustainable production of terpenoids.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Vetaka Tharasirivat, Saowarath Jantaro
Summary: The overexpression of the RuBisCO gene can increase cyanobacterial biomass and improve biocompound production. We successfully constructed two strains of Synechocystis sp. PCC 6803 that overexpressed the rbc gene, resulting in faster growth compared to the wild type strain. The increased biomass of these strains led to higher production of polyhydroxybutyrate (PHB) under nutrient-deprived conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Gang Ruan, Wujuan Mi, Xuwang Yin, Gaofei Song, Yonghong Bi
Summary: This study investigated the physiological response of Synechocystis sp. PCC 6803 to cadmium stress and identified the toxicity targets and response mechanisms. Cadmium was found to damage the cell's photosynthesis and ultrastructure. The study also identified the target sites of cadmium and revealed that the upregulation of the ABC transporter pathway and its protein expression contributed to the response mechanism to cadmium toxicity.
Article
Plant Sciences
Shoki Ito, Atsuko Watanabe, Takashi Osanai
Summary: This study biochemically characterized the L-aspartate oxidase from Synechocystis sp. PCC 6803 and determined its role in NADP+ biosynthesis under photoautotrophic conditions. The results provide important insights into the regulatory mechanism of cyanobacterial NADP+ biosynthesis.
Article
Biotechnology & Applied Microbiology
Xinyu Hu, Tianyuan Zhang, Kai Ji, Ke Luo, Li Wang, Wenli Chen
Summary: This study investigated the response of Synechocystis sp. PCC 6803 to MV-induced oxidative stress using transcriptomic and metabolomic analyses. Results showed that oxidative stress was strongest at 6 hours, with the highest proportion of upregulated genes and metabolites involved in ten metabolic pathways. Photosynthesis positively regulated the response, while environmental information processing was inhibited by MV.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Sang-Hyeok Cho, Yujin Jeong, Seong-Joo Hong, Hookeun Lee, Hyung-Kyoon Choi, Dong-Myung Kim, Choul-Gyun Lee, Suhyung Cho, Byung-Kwan Cho
Summary: This study elucidated the inhibitory mechanisms of photosynthesis in cyanobacteria under high-light and low-temperature stress conditions, highlighting the differences in response mechanisms between the two stress conditions. Through multiomics data analysis and construction of genome-wide transcription unit architecture, factors affecting photosynthesis at transcription, posttranscription, and translation levels were identified, providing pivotal information for future cyanobacterial cell factories development.
Article
Multidisciplinary Sciences
Takeshi Kanda, Genta Abiko, Yu Kanesaki, Hirofumi Yoshikawa, Noritaka Iwai, Masaaki Wachi
SCIENTIFIC REPORTS
(2020)
Article
Marine & Freshwater Biology
Yohei Shimura, Takatomo Fujisawa, Yuu Hirose, Naomi Misawa, Yu Kanesaki, Yasukazu Nakamura, Masanobu Kawachi
Summary: This study determined the complete genome sequence of the bloom-forming cyanobacterium Planktothrix agardhii, revealing similarities and differences in genomic structure with other Planktothrix strains, suggesting possible horizontal gene transfer. Additionally, gene clusters for secondary metabolites, inorganic nitrogen and phosphate uptake components, and gas vesicles were identified, contributing to a better understanding of the ecologically important genus Planktothrix.
Article
Biochemistry & Molecular Biology
Nikea J. Ulrich, Hiroko Uchida, Yu Kanesaki, Euichi Hirose, Akio Murakami, Scott R. Miller
Summary: Phenotypic plasticity through horizontal gene transfer can enhance evolutionary adaptation, promote biological diversification, and broaden ecological niches.
Article
Plant Sciences
Sota Takahashi, Riho Okubo, Yu Kanesaki, Baifeng Zhou, Kazuhiro Takaya, Satoru Watanabe, Kan Tanaka, Sousuke Imamura
Summary: This study identified four transcription factors in the unicellular red alga Cyanidioschyzon merolae that positively regulate triacylglycerol accumulation, with overexpression of LPAT1 notably increasing triacylglycerol content.
Article
Genetics & Heredity
Yuu Hirose, Yoshiyuki Ohtsubo, Naomi Misawa, Chinatsu Yonekawa, Nobuyoshi Nagao, Yohei Shimura, Takatomo Fujisawa, Yu Kanesaki, Hiroshi Katoh, Mitsunori Katayama, Haruyo Yamaguchi, Hirofumi Yoshikawa, Masahiko Ikeuchi, Toshihiko Eki, Yasukazu Nakamura, Masanobu Kawachi
Summary: Cyanobacteria are a diverse group of prokaryotes that play a crucial role in the global ecosystem. Genome sequencing of specific strains can provide insights into their genetic and phenotypic diversity, contributing to a better understanding of this important phylum.
Article
Microbiology
Hiroshi Takagi, Ryota Moriuchi, Yu Kanesaki, Satoshi Katsuyama, Masahiro Suzuki, Reo Mochizuki, Ken Yokozawa, Kenji Iwahara, Hideo Dohra
Summary: L. plantarum NMZ-1139, isolated from whisky mash, has a draft genome sequence with 3,117 protein-coding sequences, including genes associated with hop resistance like horA and hitA, making it suitable for sour beer production.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Biology
Yu Kanesaki, Mitsuo Ogura
Summary: The addition of glucose enhanced the expression of extra-cytoplasmic function sigma factors in Bacillus subtilis, and regulatory factors including YqfO, CshA, and YlxR were identified. Further analysis revealed YqfO functions as a global regulator in B. subtilis.
BMC RESEARCH NOTES
(2021)
Article
Microbiology
Naoya Kataoka, Minenosuke Matsutani, Nami Matsumoto, Misuzu Oda, Yuki Mizumachi, Kohei Ito, Shuhei Tanaka, Yu Kanesaki, Toshiharu Yakushi, Kazunobu Matsushita
Summary: Acetic acid bacterium Komagataeibacter medellinensis NBRC 3288 was adapted to higher growth temperatures through experimental evolution, resulting in improved growth and fermentation ability. The adapted strains exhibited changes in cell morphology.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Sornsiri Pattanakittivorakul, Tatsuya Tsuzuno, Tomoyuki Kosaka, Masayuki Murata, Yu Kanesaki, Hirofumi Yoshikawa, Savitree Limtong, Mamoru Yamada
Summary: This study aims to develop multi-stress tolerant strains of Kluyveromyces marxianus through a novel evolutionary adaptation procedure called RLCGT, and to increase ethanol production at high temperatures. Four adapted strains showed increased tolerance to ethanol and other stress factors, as well as higher ethanol production at high temperatures. Possible mechanisms of stress tolerance in adapted strains were discussed based on genome and transcriptome analyses.
Article
Plant Sciences
Devi B. Effendi, Toshio Sakamoto, Shuji Ohtani, Koichiro Awai, Yu Kanesaki
Summary: Cyanobacteria strain Nostoc sp. SO-36, isolated from Antarctica, was found to be psychrotolerant and filamentous when grown at 30 degrees Celsius, with the ability to grow diazotrophically. At 10 degrees Celsius, the cells showed reduced growth, smaller size, and increased aggregation with extracellular polymeric substrates (EPSs) to protect from UV damage. The complete genome sequencing revealed the presence of one main chromosome with plasmids and conserved genes for EPS biosynthesis.
JOURNAL OF PLANT RESEARCH
(2022)
Article
Multidisciplinary Sciences
Takafumi Maruyama, Satoru Sumi, Mitsuru Kobayashi, Teppei Ebuchi, Yu Kanesaki, Hirofumi Yoshikawa, Kenji Ueda, Hideaki Takano
Summary: PmlR2 is shown to be an effector protein of PmSB-LOV. The direct association between PmSB-LOV and PmlR2 inhibits the DNA-binding activity of PmlR2, enabling transcription of light-inducible promoters.
SCIENTIFIC REPORTS
(2022)
Article
Ecology
Ryo Matsuzaki, Shigekatsu Suzuki, Haruyo Yamaguchi, Masanobu Kawachi, Yu Kanesaki, Hirofumi Yoshikawa, Toshiyuki Mori, Hisayoshi Nozaki
Summary: The study suggests that the hydrophobicity of RBCS helices is roughly correlated with the presence or absence of pyrenoids within the Reticulata group, and a decrease in hydrophobicity of RBCS helices may have primarily caused pyrenoid loss during the evolution of this group. This provides novel insight into a potential mechanism determining initial evolutionary steps of gain and loss of the pyrenoid.
BMC ECOLOGY AND EVOLUTION
(2021)
Article
Biochemistry & Molecular Biology
Ryota Moriuchi, Hideo Dohra, Yu Kanesaki, Naoto Ogawa
Summary: RNA-seq analysis of Cupriavidus necator NH9 cultured with 3-chlorobenzoate and benzoate revealed distinct gene expression patterns and strong enzyme induction. NH9 showed higher adaptability to benzoate than to 3-chlorobenzoate in terms of ortho-cleavage pathway and chemotaxis.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Microbiology
Toshihide Iwatsuki, Takahiro Kanazawa, Takato Ogasawara, Kento Hosotani, Karen Tsuchiya, Shinichi Watanabe, Tomoko Suzuki, Ryota Moriuchi, Yu Kanesaki, Hideo Dohra
Summary: The 16S rRNA gene amplicon sequence analysis of the gut microbiota in three species of deep-sea fish from Suruga Bay, Japan revealed that two species were dominated by the phylum Proteobacteria (genus Photobacterium), while one species was dominated by the phyla Spirochaetes (genus Brevinema) and Tenericutes (unclassified Mycoplasmataceae).
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Biotechnology & Applied Microbiology
Shinya Yoshikawa, Yu Kanesaki, Akira Uemura, Kazumasa Yamada, Maiko Okajima, Tatsuo Kaneko, Kaori Ohki
Summary: In this study, a unicellular cyanobacterium named Chroococcus sp. FPU101 was isolated, which produces a significant amount of exopolysaccharide (EPS). The research determined the growth conditions and chemical composition of EPS produced by Chroococcus sp. FPU101. Results indicated that an increase in NaCl concentration significantly boosted EPS content in the organism.
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
(2021)