Structural and functional diversity of type IV secretion systems

Mechanism of regulation of the Helicobacter pylori Cagβ ATPase by CagZ

(2023) Xiuling Wu et al.   Nature Communications

Agrobacterium tumefaciens: a Transformative Agent for Fundamental Insights into Host-Microbe Interactions, Genome Biology, Chemical Signaling, and Cell Biology

(2023) Pamela J. B. Brown et al.   JOURNAL OF BACTERIOLOGY

Cryo-EM structure of the Agrobacterium tumefaciens T4SS-associated T-pilus reveals stoichiometric protein-phospholipid assembly

(2023) Stefan Kreida et al.   STRUCTURE

Substrate recruitment mechanism by gram-negative type III, IV, and VI bacterial injectisomes

(2023) Amit Meir et al.   TRENDS IN MICROBIOLOGY

The F-pilus biomechanical adaptability accelerates conjugative dissemination of antimicrobial resistance and biofilm formation

(2023) Jonasz B. Patkowski et al.   Nature Communications

Archaeal DNA-import apparatus is homologous to bacterial conjugation machinery

(2023) Leticia C. Beltran et al.   Nature Communications

Structural basis for effector recognition by an antibacterial type IV secretion system

(2022) Gabriel U. Oka et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of a type IV secretion system core complex encoded by multi-drug resistance F plasmids

(2022) Xiangan Liu et al.   Nature Communications

The Legionella pneumophila Dot/Icm type IV secretion system and its effectors

(2022) Daniel C. Lockwood et al.   MICROBIOLOGY-SGM

Cryo-EM structure of a type IV secretion system

(2022) Kévin Macé et al.   NATURE

Molecular architecture of bacterial type IV secretion systems

(2022) Michael J. Sheedlo et al.   PLoS Pathogens

Developmental Transitions Coordinate Assembly of the Coxiella burnetii Dot/Icm Type IV Secretion System

(2022) Donghyun Park et al.   INFECTION AND IMMUNITY

Targeted bacterial conjugation mediated by synthetic cell-to-cell adhesions

(2022) Marta Robledo et al.   NUCLEIC ACIDS RESEARCH

Cryo-EM structure of the Agrobacterium tumefaciens T-pilus reveals the importance of positive charges in the lumen

(2022) Jaafar Amro et al.   STRUCTURE

Targeted-antibacterial-plasmids (TAPs) combining conjugation and CRISPR/Cas systems achieve strain-specific antibacterial activity

(2021) Audrey Reuter et al.   NUCLEIC ACIDS RESEARCH

Driving to Safety: CRISPR-Based Genetic Approaches to Reducing Antibiotic Resistance

(2021) Ethan Bier et al.   TRENDS IN GENETICS

Secretion of Pertussis Toxin from Bordetella pertussis

(2021) Drusilla L. Burns   Toxins

Cryo-EM reveals new species-specific proteins and symmetry elements in the Legionella pneumophila Dot/Icm T4SS

(2021) Michael J Sheedlo et al.   eLife

Recent advances in structural studies of the Legionella pneumophila Dot/Icm type IV secretion system

(2021) Tomoe Kitao et al.   MICROBIOLOGY AND IMMUNOLOGY

Proteins DotY and DotZ modulate the dynamics and localization of the type IVB coupling complex of Legionella pneumophila

(2021) Kevin Macé et al.   MOLECULAR MICROBIOLOGY

In Situ Visualization of the pKM101-Encoded Type IV Secretion System Reveals a Highly Symmetric ATPase Energy Center

(2021) Pratick Khara et al.   mBio

Architecture of the outer-membrane core complex from a conjugative type IV secretion system

(2021) Himani Amin et al.   Nature Communications

Analysis of Dot/Icm Type IVB Secretion System Subassemblies by Cryoelectron Tomography Reveals Conformational Changes Induced by DotB Binding

(2020) Donghyun Park et al.   mBio

Electrostatic Switching Controls Channel Dynamics of the Sensor Protein VirB10 in A. tumefaciens Type IV Secretion System

(2020) Vidya Chandran Darbari et al.   ACS Omega

The Helicobacter pylori Cag Type IV Secretion System

(2020) Timothy L. Cover et al.   TRENDS IN MICROBIOLOGY

Structural basis for effector protein recognition by the Dot/Icm Type IVB coupling protein complex

(2020) Hyunmin Kim et al.   Nature Communications

Targeting Plasmids to Limit Acquisition and Transmission of Antimicrobial Resistance

(2020) Corneliu Ovidiu Vrancianu et al.   Frontiers in Microbiology

Mechanism of effector capture and delivery by the type IV secretion system from Legionella pneumophila

(2020) Amit Meir et al.   Nature Communications

Type IV Coupling Proteins as Potential Targets to Control the Dissemination of Antibiotic Resistance

(2020) Itxaso Álvarez-Rodríguez et al.   Frontiers in Molecular Biosciences

Cryoelectron-Microscopic Structure of the pKpQIL Conjugative Pili from Carbapenem-Resistant Klebsiella pneumoniae

(2020) Weili Zheng et al.   STRUCTURE

Toll-like Receptor 5 Activation by the CagY Repeat Domains of Helicobacter pylori

(2020) Nicole Tegtmeyer et al.   Cell Reports

Structural analysis of the Legionella pneumophila Dot/Icm type IV secretion system core complex

(2020) Clarissa L Durie et al.   eLife

Cryo-EM reveals species-specific components within the Helicobacter pylori Cag type IV secretion system core complex

(2020) Michael J Sheedlo et al.   eLife

Type IV secretion systems: Advances in structure, function, and activation

(2020) Tiago R. D. Costa et al.   MOLECULAR MICROBIOLOGY

From conjugation to T4S systems in Gram‐negative bacteria: a mechanistic biology perspective

(2019) Gabriel Waksman   EMBO REPORTS

More than 18,000 effectors in the Legionella genus genome provide multiple, independent combinations for replication in human cells

(2019) Laura Gomez-Valero et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

ADP heptose, a novel pathogen-associated molecular pattern identified in Helicobacter pylori

(2019) Lennart Pfannkuch et al.   FASEB JOURNAL

In Situ Molecular Architecture of the Helicobacter pylori Cag Type IV Secretion System

(2019) Bo Hu et al.   mBio

Bacteria-Killing Type IV Secretion Systems

(2019) Germán G. Sgro et al.   Frontiers in Microbiology

Structural bases for F plasmid conjugation and F pilus biogenesis inEscherichia coli

(2019) Bo Hu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Fragment-based screening identifies inhibitors of ATPase activity and of hexamer formation of Cagα from the Helicobacter pylori type IV secretion system

(2019) Tarun Arya et al.   Scientific Reports

Structure of the Helicobacter pylori Cag type IV secretion system

(2019) Jeong Min Chung et al.   eLife

Efficient inter-species conjugative transfer of a CRISPR nuclease for targeted bacterial killing

(2019) Thomas A. Hamilton et al.   Nature Communications

Plasmids carrying antimicrobial resistance genes in Enterobacteriaceae

(2018) M Rozwandowicz et al.   JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

X-ray crystal structures of the type IVb secretion system DotB ATPases

(2018) Marie S. Prevost et al.   PROTEIN SCIENCE

CagY-Dependent Regulation of Type IV Secretion in Helicobacter pylori Is Associated with Alterations in Integrin Binding

(2018) Emma C. Skoog et al.   mBio

Legionella DotM structure reveals a role in effector recruiting to the Type 4B secretion system

(2018) Amit Meir et al.   Nature Communications

In Vivo Structures of the Helicobacter pylori cag Type IV Secretion System

(2018) Yi-Wei Chang et al.   Cell Reports

A unique cytoplasmic ATPase complex defines the Legionella pneumophila type IV secretion channel

(2018) David Chetrit et al.   Nature Microbiology

Conjugation inhibitors compete with palmitic acid for binding to the conjugative traffic ATPase TrwD, providing a mechanism to inhibit bacterial conjugation

(2018) Yolanda García-Cazorla et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Cryo-EM structure of the bacteria-killing type IV secretion system core complex from Xanthomonas citri

(2018) Germán G. Sgro et al.   Nature Microbiology

Subversion of host kinases: a key network in cellular signaling hijacked by Helicobacter pylori CagA

(2017) Nicole Tegtmeyer et al.   MOLECULAR MICROBIOLOGY

Use of chimeric type IV secretion systems to define contributions of outer membrane subassemblies for contact-dependent translocation

(2017) Jay E. Gordon et al.   MOLECULAR MICROBIOLOGY

A comprehensive guide to pilus biogenesis in Gram-negative bacteria

(2017) Manuela K. Hospenthal et al.   NATURE REVIEWS MICROBIOLOGY

Structural insights into the roles of the IcmS–IcmW complex in the type IVb secretion system ofLegionella pneumophila

(2017) Jianpo Xu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Fragment-based screening identifies novel targets for inhibitors of conjugative transfer of antimicrobial resistance by plasmid pKM101

(2017) Bastien Casu et al.   Scientific Reports

Conjugation Inhibitors and Their Potential Use to Prevent Dissemination of Antibiotic Resistance Genes in Bacteria

(2017) Elena Cabezón et al.   Frontiers in Microbiology

Architecture of the type IV coupling protein complex of Legionella pneumophila

(2017) Mi-Jeong Kwak et al.   Nature Microbiology

Structure of the Bacterial Sex F Pilus Reveals an Assembly of a Stoichiometric Protein-Phospholipid Complex

(2016) Tiago R.D. Costa et al.   CELL

Type IV traffic ATPase TrwD as molecular target to inhibit bacterial conjugation

(2016) Jorge Ripoll-Rozada et al.   MOLECULAR MICROBIOLOGY

Pathogenic Helicobacter pylori strains translocate DNA and activate TLR9 via the cancer-associated cag type IV secretion system

(2016) M G Varga et al.   ONCOGENE

Molecular and Structural Analysis of the Helicobacter pylori cag Type IV Secretion System Core Complex

(2016) Arwen E. Frick-Cheng et al.   mBio

Peptidomimetic Small Molecules Disrupt Type IV Secretion System Activity in Diverse Bacterial Pathogens

(2016) Carrie L. Shaffer et al.   mBio

Systematic site-directed mutagenesis of the Helicobacter pylori CagL protein of the Cag type IV secretion system identifies novel functional domains

(2016) Tobias Bönig et al.   Scientific Reports

The All-Alpha Domains of Coupling Proteins from the Agrobacterium tumefaciens VirB/VirD4 and Enterococcus faecalis pCF10-Encoded Type IV Secretion Systems Confer Specificity to Binding of Cognate DNA Substrates

(2015) Neal Whitaker et al.   JOURNAL OF BACTERIOLOGY

Novel export control of aLegionella Dot/Icm substrate is mediated by dual, independent signal sequences

(2015) Kwang Cheol Jeong et al.   MOLECULAR MICROBIOLOGY

Secretion systems in Gram-negative bacteria: structural and mechanistic insights

(2015) Tiago R. D. Costa et al.   NATURE REVIEWS MICROBIOLOGY

Bacterial Pathogen Manipulation of Host Membrane Trafficking

(2014) Seblewongel Asrat et al.   Annual Review of Cell and Developmental Biology

Structure of a type IV secretion system

(2014) Harry H. Low et al.   NATURE

Structure of a bacterial type IV secretion core complex at subnanometre resolution

(2013) Angel Rivera-Calzada et al.   EMBO JOURNAL

DNA Substrate-Induced Activation of the Agrobacterium VirB/VirD4 Type IV Secretion System

(2013) E. Cascales et al.   JOURNAL OF BACTERIOLOGY

Functional Interactions of VirB11 Traffic ATPases with VirB4 and VirD4 Molecular Motors in Type IV Secretion Systems

(2013) J. Ripoll-Rozada et al.   JOURNAL OF BACTERIOLOGY

Effector Protein Translocation by the Coxiella burnetii Dot/Icm Type IV Secretion System Requires Endocytic Maturation of the Pathogen-Occupied Vacuole

(2013) Hayley J. Newton et al.   PLoS One

Functional Plasticity in the Type IV Secretion System of Helicobacter pylori

(2013) Roberto M. Barrozo et al.   PLoS Pathogens

Identification of the DotL coupling protein subcomplex of the Legionella Dot/Icm type IV secretion system

(2012) Carr D. Vincent et al.   MOLECULAR MICROBIOLOGY

CagI Is an Essential Component of the Helicobacter pylori Cag Type IV Secretion System and Forms a Complex with CagL

(2012) Kieu Thuy Pham et al.   PLoS One

An Agrobacterium VirB10 Mutation Conferring a Type IV Secretion System Gating Defect

(2011) L. M. Banta et al.   JOURNAL OF BACTERIOLOGY

Structural basis of cooperative DNA recognition by the plasmid conjugation factor, TraM

(2011) Joyce J. W. Wong et al.   NUCLEIC ACIDS RESEARCH

A Component of the Xanthomonadaceae Type IV Secretion System Combines a VirB7 Motif with a N0 Domain Found in Outer Membrane Transport Proteins

(2011) Diorge P. Souza et al.   PLoS Pathogens

Two-step and one-step secretion mechanisms in Gram-negative bacteria: contrasting the type IV secretion system and the chaperone-usher pathway of pilus biogenesis

(2010) Ana Toste Rêgo et al.   BIOCHEMICAL JOURNAL

AnIn VivoHigh-Throughput Screening Approach Targeting the Type IV Secretion System Component VirB8 Identified Inhibitors ofBrucella abortus2308 Proliferation

(2010) Athanasios Paschos et al.   INFECTION AND IMMUNITY

Phylogenomics Reveals a Diverse Rickettsiales Type IV Secretion System

(2010) J. J. Gillespie et al.   INFECTION AND IMMUNITY

Crystal Structure of Legionella DotD: Insights into the Relationship between Type IVB and Type II/III Secretion Systems

(2010) Noboru Nakano et al.   PLoS Pathogens

Biological Diversity of Prokaryotic Type IV Secretion Systems

(2009) C. E. Alvarez-Martinez et al.   MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS

Structure of the outer membrane complex of a type IV secretion system

(2009) Vidya Chandran et al.   NATURE

An Anomalous Type IV Secretion System in Rickettsia Is Evolutionarily Conserved

(2009) Joseph J. Gillespie et al.   PLoS One

Structure of a Type IV Secretion System Core Complex

(2009) R. Fronzes et al.   SCIENCE

Genetic and Functional Characterization of the Type IV Secretion System in Wolbachia

(2008) E. Rances et al.   JOURNAL OF BACTERIOLOGY

Structural basis of specific TraD-TraM recognition during F plasmid-mediated bacterial conjugation

(2008) Jun Lu et al.   MOLECULAR MICROBIOLOGY

VirB2 and VirB5 proteins: specialized adhesins in bacterial type-IV secretion systems?

(2008) Steffen Backert et al.   TRENDS IN MICROBIOLOGY