Driving to Safety: CRISPR-Based Genetic Approaches to Reducing Antibiotic Resistance

Inherently confinable split-drive systems in Drosophila

(2021) Gerard Terradas et al.   Nature Communications

Anti-CRISPRs: Protein Inhibitors of CRISPR-Cas Systems

(2020) Alan R. Davidson et al.   Annual Review of Biochemistry

Vesiduction: the fourth way of HGT

(2020) Nicolas Soler et al.   ENVIRONMENTAL MICROBIOLOGY

A male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiae

(2020) Alekos Simoni et al.   NATURE BIOTECHNOLOGY

On-target activity predictions enable improved CRISPR–dCas9 screens in bacteria

(2020) Alicia Calvo-Villamañán et al.   NUCLEIC ACIDS RESEARCH

Toward the Definition of Efficacy and Safety Criteria for Advancing Gene Drive-Modified Mosquitoes to Field Testing

(2020) Stephanie L. James et al.   VECTOR-BORNE AND ZOONOTIC DISEASES

In Vivo Targeting of Clostridioides difficile Using Phage-Delivered CRISPR-Cas3 Antimicrobials

(2020) Kurt Selle et al.   mBio

One-Day Construction of Multiplex Arrays to Harness Natural CRISPR-Cas Systems

(2020) Robert M. Cooper et al.   ACS Synthetic Biology

Multiplex Generation, Tracking, and Functional Screening of Substitution Mutants Using a CRISPR/Retron System

(2020) Hyeonseob Lim et al.   ACS Synthetic Biology

Broad-spectrum anti-CRISPR proteins facilitate horizontal gene transfer

(2020) Caroline Mahendra et al.   Nature Microbiology

Safety Aspects of Genetically Modified Lactic Acid Bacteria

(2020) Tina Vida Plavec et al.   Microorganisms

Beyond the CRISPR-Cas safeguard: PICI-encoded innate immune systems protect bacteria from bacteriophage predation

(2020) Alfred Fillol-Salom et al.   CURRENT OPINION IN MICROBIOLOGY

Development of CRISPR-Cas13a-based antimicrobials capable of sequence-specific killing of target bacteria

(2020) Kotaro Kiga et al.   Nature Communications

Active Genetic Neutralizing Elements for Halting or Deleting Gene Drives

(2020) Xiang-Ru Shannon Xu et al.   MOLECULAR CELL

ssRNA phage penetration triggers detachment of the F-pilus

(2020) Laith Harb et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Next-generation gene drive for population modification of the malaria vector mosquito, Anopheles gambiae

(2020) Rebeca Carballar-Lejarazú et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Diverse enzymatic activities mediate antiviral immunity in prokaryotes

(2020) Linyi Gao et al.   SCIENCE

Bacterial Retrons Function In Anti-Phage Defense

(2020) Adi Millman et al.   CELL

Metagenomic engineering of the mammalian gut microbiome in situ

(2019) Carlotta Ronda et al.   NATURE METHODS

Super-Mendelian inheritance mediated by CRISPR–Cas9 in the female mouse germline

(2019) Hannah A. Grunwald et al.   NATURE

Engineered toxin–intein antimicrobials can selectively target and kill antibiotic-resistant bacteria in mixed populations

(2019) Rocío López-Igual et al.   NATURE BIOTECHNOLOGY

Regulation of Gram-Positive Conjugation

(2019) Verena Kohler et al.   Frontiers in Microbiology

Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integration

(2019) Sanne E. Klompe et al.   NATURE

Sustainable Discovery and Development of Antibiotics — Is a Nonprofit Approach the Future?

(2019) Travis B. Nielsen et al.   NEW ENGLAND JOURNAL OF MEDICINE

Delineation of the integrase-attachment and origin-of-transfer regions of the symbiosis island ICEMlSymR7A

(2019) Callum J. Verdonk et al.   PLASMID

Multiple origins of reverse transcriptases linked to CRISPR-Cas systems

(2019) Nicolás Toro et al.   RNA Biology

RNA-guided DNA insertion with CRISPR-associated transposases

(2019) Jonathan Strecker et al.   SCIENCE

Efficient allelic-drive in Drosophila

(2019) Annabel Guichard et al.   Nature Communications

Enterococcus faecalis CRISPR-Cas Is a Robust Barrier to Conjugative Antibiotic Resistance Dissemination in the Murine Intestine

(2019) Valerie J. Price et al.   mSphere

Antibiotic Pollution in the Environment: From Microbial Ecology to Public Policy

(2019) Susanne A. Kraemer et al.   Microorganisms

Conjugative delivery of CRISPR-Cas9 for the selective depletion of antibiotic-resistant enterococci

(2019) Marinelle Rodrigues et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

A review of bacteriophage therapy for pathogenic bacteria inactivation in the soil environment

(2019) Mao Ye et al.   ENVIRONMENT INTERNATIONAL

Mathematical modelling to study the horizontal transfer of antimicrobial resistance genes in bacteria: current state of the field and recommendations

(2019) Quentin J. Leclerc et al.   Journal of the Royal Society Interface

Gene silencing with CRISPRi in bacteria and optimization of dCas9 expression levels

(2019) Florence Depardieu et al.   METHODS

Molecular epidemiology and mechanisms of antibiotic resistance in Enterococcus spp., Staphylococcus spp., and Streptococcus spp. in Africa: a systematic review from a One Health perspective

(2019) John Osei Sekyere et al.   Annals of the New York Academy of Sciences

Antibiotics: past, present and future

(2019) Matthew I Hutchings et al.   CURRENT OPINION IN MICROBIOLOGY

Evolutionary entanglement of mobile genetic elements and host defence systems: guns for hire

(2019) Eugene V. Koonin et al.   NATURE REVIEWS GENETICS

Efficient inter-species conjugative transfer of a CRISPR nuclease for targeted bacterial killing

(2019) Thomas A. Hamilton et al.   Nature Communications

Porins and small-molecule translocation across the outer membrane of Gram-negative bacteria

(2019) Julia Vergalli et al.   NATURE REVIEWS MICROBIOLOGY

Antibiotic resistance genes identified in wastewater treatment plant systems – A review

(2019) Magdalena Pazda et al.   SCIENCE OF THE TOTAL ENVIRONMENT

A bacterial gene-drive system efficiently edits and inactivates a high copy number antibiotic resistance locus

(2019) J. Andrés Valderrama et al.   Nature Communications

Assessment of a Split Homing Based Gene Drive for Efficient Knockout of Multiple Genes

(2019) Nikolay P. Kandul et al.   G3-Genes Genomes Genetics

Pathway to Deployment of Gene Drive Mosquitoes as a Potential Biocontrol Tool for Elimination of Malaria in Sub-Saharan Africa: Recommendations of a Scientific Working Group †

(2018) Stephanie James et al.   AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE

Understanding Biological Regulation Through Synthetic Biology

(2018) Caleb J. Bashor et al.   Annual Review of Biophysics

Transmission ratio distortion is frequent in Arabidopsis thaliana controlled crosses

(2018) Danelle K. Seymour et al.   HEREDITY

Sub-inhibitory concentrations of fluoroquinolones increase conjugation frequency

(2018) E. Shun-Mei et al.   MICROBIAL PATHOGENESIS

Type IV secretion in Gram-negative and Gram-positive bacteria

(2018) Elisabeth Grohmann et al.   MOLECULAR MICROBIOLOGY

Next-generation biocontainment systems for engineered organisms

(2018) Jeong Wook Lee et al.   Nature Chemical Biology

Destabilizing mutations encode nongenetic variation that drives evolutionary innovation

(2018) Katherine L. Petrie et al.   SCIENCE

CRISPR-Cas-Mediated Phage Resistance Enhances Horizontal Gene Transfer by Transduction

(2018) Bridget N. J. Watson et al.   mBio

Human evolutionary loss of epithelial Neu5Gc expression and species-specific susceptibility to cholera

(2018) Frederico Alisson-Silva et al.   PLoS Pathogens

Horizontal transfer of antibiotic resistance genes in clinical environments

(2018) Nicole A. Lerminiaux et al.   CANADIAN JOURNAL OF MICROBIOLOGY

A CRISPR–Cas9 gene drive targeting doublesex causes complete population suppression in caged Anopheles gambiae mosquitoes

(2018) Kyros Kyrou et al.   NATURE BIOTECHNOLOGY

Conversion of staphylococcal pathogenicity islands to CRISPR-carrying antibacterial agents that cure infections in mice

(2018) Geeta Ram et al.   NATURE BIOTECHNOLOGY

Engineered integrative and conjugative elements for efficient and inducible DNA transfer to undomesticated bacteria

(2018) Jennifer A. N. Brophy et al.   Nature Microbiology

Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors

(2018) François Rousset et al.   PLoS Genetics

Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi

(2018) Jason M. Peters et al.   Nature Microbiology

Genetic conflicts: the usual suspects and beyond

(2017) Richard N. McLaughlin et al.   JOURNAL OF EXPERIMENTAL BIOLOGY

Rules of the road for insect gene drive research and testing

(2017) Zach Adelman et al.   NATURE BIOTECHNOLOGY

Recruitment of CRISPR-Cas systems by Tn7-like transposons

(2017) Joseph E. Peters et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Pharmacological Targeting of the Host–Pathogen Interaction: Alternatives to Classical Antibiotics to Combat Drug-Resistant Superbugs

(2017) Jason Munguia et al.   TRENDS IN PHARMACOLOGICAL SCIENCES

Inter-species population dynamics enhance microbial horizontal gene transfer and spread of antibiotic resistance

(2017) Robert M Cooper et al.   eLife

CRISPR/Cas9 and active genetics-based trans-species replacement of the endogenous Drosophila kni-L2 CRM reveals unexpected complexity

(2017) Xiang-Ru Shannon Xu et al.   eLife

Quorum Sensing N-acyl Homoserine Lactones-SdiA Suppresses Escherichia coli-Pseudomonas aeruginosa Conjugation through Inhibiting traI Expression

(2017) Yang Lu et al.   Frontiers in Cellular and Infection Microbiology

Global epidemiology of urinary tract infections

(2016) Zafer Tandogdu et al.   CURRENT OPINION IN INFECTIOUS DISEASES

Reexamining the P -Element Invasion of Drosophila melanogaster Through the Lens of piRNA Silencing

(2016) Erin S. Kelleher   GENETICS

CRISPR/Cas9-Mediated Re-Sensitization of Antibiotic-Resistant Escherichia coli Harboring Extended-Spectrum ��-Lactamases

(2016) Jun-Seob Kim et al.   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

Understanding the mechanisms and drivers of antimicrobial resistance

(2016) Alison H Holmes et al.   LANCET

Averting a malaria disaster: will insecticide resistance derail malaria control?

(2016) Janet Hemingway et al.   LANCET

Plasmid-like replication of a minimal streptococcal integrative and conjugative element

(2016) Nicolas Carraro et al.   MICROBIOLOGY-SGM

Ecological speciation of bacteriophage lambda in allopatry and sympatry

(2016) J. R. Meyer et al.   SCIENCE

Leveraging antimicrobial stewardship into improving rates of carbapenem-resistant Enterobacteriaceae

(2016) Darren Wong et al.   Virulence

Dissemination of Antimicrobial Resistance in Microbial Ecosystems through Horizontal Gene Transfer

(2016) Christian J. H. von Wintersdorff et al.   Frontiers in Microbiology

Reexamining the P -Element Invasion of Drosophila melanogaster Through the Lens of piRNA Silencing

(2016) Erin S. Kelleher   GENETICS

The dawn of active genetics

(2015) Valentino M. Gantz et al.   BIOESSAYS

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

The structural biology of CRISPR-Cas systems

(2015) Fuguo Jiang et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

No evidence of inhibition of horizontal gene transfer by CRISPR–Cas on evolutionary timescales

(2015) Uri Gophna et al.   ISME Journal

Expanding the Biologist’s Toolkit with CRISPR-Cas9

(2015) Samuel H. Sternberg et al.   MOLECULAR CELL

In vivo genome editing using Staphylococcus aureus Cas9

(2015) F. Ann Ran et al.   NATURE

Safeguarding CRISPR-Cas9 gene drives in yeast

(2015) James E DiCarlo et al.   NATURE BIOTECHNOLOGY

A CRISPR-Cas9 gene drive system targeting female reproduction in the malaria mosquito vector Anopheles gambiae

(2015) Andrew Hammond et al.   NATURE BIOTECHNOLOGY

Antibiotic resistance genes in the environment: prioritizing risks

(2015) Johan Bengtsson-Palme et al.   NATURE REVIEWS MICROBIOLOGY

Temperate and lytic bacteriophages programmed to sensitize and kill antibiotic-resistant bacteria

(2015) Ido Yosef et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Highly efficient Cas9-mediated gene drive for population modification of the malaria vector mosquitoAnopheles stephensi

(2015) Valentino M. Gantz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The mutagenic chain reaction: A method for converting heterozygous to homozygous mutations

(2015) V. M. Gantz et al.   SCIENCE

Biophysical mechanisms that maintain biodiversity through trade-offs

(2015) Justin R. Meyer et al.   Nature Communications

Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases

(2014) Robert J Citorik et al.   NATURE BIOTECHNOLOGY

Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials

(2014) David Bikard et al.   NATURE BIOTECHNOLOGY

RAB11-mediated trafficking in host–pathogen interactions

(2014) Annabel Guichard et al.   NATURE REVIEWS MICROBIOLOGY

Genomically encoded analog memory with precise in vivo DNA writing in living cell populations

(2014) F. Farzadfard et al.   SCIENCE

Positive selection and compensatory adaptation interact to stabilize non-transmissible plasmids

(2014) A. San Millan et al.   Nature Communications

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Dealing with the Evolutionary Downside of CRISPR Immunity: Bacteria and Beneficial Plasmids

(2013) Wenyan Jiang et al.   PLoS Genetics

Correction: Unique Biofilm Signature, Drug Susceptibility and Decreased Virulence in Drosophila through the Pseudomonas aeruginosa Two-Component System PprAB

(2013) Sophie de Bentzmann et al.   PLoS Pathogens

Human Transposon Tectonics

(2012) Kathleen H. Burns et al.   CELL

CRISPR Interference Can Prevent Natural Transformation and Virulence Acquisition during In Vivo Bacterial Infection

(2012) David Bikard et al.   Cell Host & Microbe

Regulatory circuits controlling enterococcal conjugation: lessons for functional genomics

(2011) Gary M Dunny et al.   CURRENT OPINION IN MICROBIOLOGY

Deconstructing host-pathogen interactions in Drosophila

(2011) E. Bier et al.   Disease Models & Mechanisms

New insights into the biological effects of anthrax toxins: linking cellular to organismal responses

(2011) Annabel Guichard et al.   MICROBES AND INFECTION

Rapid Rise and Fall of Selfish Sex-Ratio X Chromosomes in Drosophila simulans: Spatiotemporal Analysis of Phenotypic and Molecular Data

(2011) Héloïse Bastide et al.   MOLECULAR BIOLOGY AND EVOLUTION

Spatially selective colonization of the arthropod intestine through activation of Vibrio cholerae biofilm formation

(2011) A. E. Purdy et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Drosophila melanogaster as an Animal Model for the Study of Pseudomonas aeruginosa Biofilm Infections In Vivo

(2011) Heidi Mulcahy et al.   PLoS Pathogens

Mobility of Plasmids

(2010) C. Smillie et al.   MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS

Transposable elements in natural populations of Drosophila melanogaster

(2010) Y. C. G. Lee et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Recurrent Selection on the Winters sex-ratio Genes in Drosophila simulans

(2009) S. B. Kingan et al.   GENETICS

Large-Scale Selective Sweep among Segregation Distorter Chromosomes in African Populations of Drosophila melanogaster

(2009) Daven C. Presgraves et al.   PLoS Genetics

CRISPR Interference Limits Horizontal Gene Transfer in Staphylococci by Targeting DNA

(2008) Luciano A. Marraffini et al.   SCIENCE