Targeted bacterial conjugation mediated by synthetic cell-to-cell adhesions

4-bit adhesion logic enables universal multicellular interface patterning

(2022) Honesty Kim et al.   NATURE

Mating pair stabilization mediates bacterial conjugation species specificity

(2022) Wen Wen Low et al.   Nature Microbiology

High‐efficiency delivery of CRISPR‐Cas9 by engineered probiotics enables precise microbiome editing

(2021) Kevin Neil et al.   Molecular Systems Biology

Mucins and the Microbiome

(2020) Gunnar C. Hansson   Annual Review of Biochemistry

Targeted Depletion of Bacteria from Mixed Populations by Programmable Adhesion with Antagonistic Competitor Cells

(2020) See-Yeun Ting et al.   Cell Host & Microbe

Mucins in Intestinal Mucosal Defense and Inflammation: Learning From Clinical and Experimental Studies

(2020) Jensine A. Grondin et al.   Frontiers in Immunology

From conjugation to T4S systems in Gram‐negative bacteria: a mechanistic biology perspective

(2019) Gabriel Waksman   EMBO REPORTS

Metagenomic engineering of the mammalian gut microbiome in situ

(2019) Carlotta Ronda et al.   NATURE METHODS

Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system

(2019) David Ruano-Gallego et al.   Microbial Cell Factories

Engineered toxin–intein antimicrobials can selectively target and kill antibiotic-resistant bacteria in mixed populations

(2019) Rocío López-Igual et al.   NATURE BIOTECHNOLOGY

A nanobody targeting the translocated intimin receptor inhibits the attachment of enterohemorrhagic E. coli to human colonic mucosa

(2019) David Ruano-Gallego et al.   PLoS Pathogens

A Synthetic Bacterial Cell-Cell Adhesion Toolbox for Programming Multicellular Morphologies and Patterns

(2018) David S. Glass et al.   CELL

Two pKM101-Encoded Proteins, the Pilus-Tip Protein TraC and Pep, Assemble on the Escherichia coli Cell Surface as Adhesins Required for Efficient Conjugative DNA Transfer

(2018) Christian Gonzalez-Rivera et al.   MOLECULAR MICROBIOLOGY

Conversion of staphylococcal pathogenicity islands to CRISPR-carrying antibacterial agents that cure infections in mice

(2018) Geeta Ram et al.   NATURE BIOTECHNOLOGY

Engineered integrative and conjugative elements for efficient and inducible DNA transfer to undomesticated bacteria

(2018) Jennifer A. N. Brophy et al.   Nature Microbiology

Synthetic consortia of nanobody-coupled and formatted bacteria for prophylaxis and therapy interventions targeting microbiome dysbiosis-associated diseases and co-morbidities

(2018) Kenneth Timmis et al.   Microbial Biotechnology

Conjugation efficiency depends on intra and intercellular interactions between distinct plasmids: Plasmids promote the immigration of other plasmids but repress co-colonizing plasmids

(2017) João Alves Gama et al.   PLASMID

PifC and Osa, Plasmid Weapons against Rival Conjugative Coupling Proteins

(2017) María Getino et al.   Frontiers in Microbiology

Escherichia coli surface display for the selection of nanobodies

(2017) Valencio Salema et al.   Microbial Biotechnology

Manipulating Bacterial Communities by in situ Microbiome Engineering

(2016) Ravi U. Sheth et al.   TRENDS IN GENETICS

Fate of ingested fluids: factors affecting gastric emptying and intestinal absorption of beverages in humans

(2015) John B. Leiper   NUTRITION REVIEWS

Elucidating the Crucial Role of Poly N-Acetylglucosamine from Staphylococcus aureus in Cellular Adhesion and Pathogenesis

(2015) Mei Hui Lin et al.   PLoS One

Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases

(2014) Robert J Citorik et al.   NATURE BIOTECHNOLOGY

Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials

(2014) David Bikard et al.   NATURE BIOTECHNOLOGY

Programming Controlled Adhesion of E. coli to Target Surfaces, Cells, and Tumors with Synthetic Adhesins

(2014) Carlos Piñero-Lambea et al.   ACS Synthetic Biology

Nanobodies: Natural Single-Domain Antibodies

(2013) Serge Muyldermans   Annual Review of Biochemistry

Selection of Single Domain Antibodies from Immune Libraries Displayed on the Surface of E. coli Cells with Two β-Domains of Opposite Topologies

(2013) Valencio Salema et al.   PLoS One

Determination of conjugation rates on solid surfaces

(2012) Irene del Campo et al.   PLASMID

Crystal Structures of the Outer Membrane Domain of Intimin and Invasin from Enterohemorrhagic E. coli and Enteropathogenic Y. pseudotuberculosis

(2012) James W. Fairman et al.   STRUCTURE

Enteropathogenic and enterohaemorrhagic Escherichia coli: even more subversive elements

(2011) Alexander R. C. Wong et al.   MOLECULAR MICROBIOLOGY

Comparative Analysis of the Biochemical and Functional Properties of C-Terminal Domains of Autotransporters

(2010) E. Marin et al.   JOURNAL OF BACTERIOLOGY

Crystal Structure of EHEC Intimin: Insights into the Complementarity between EPEC and EHEC

(2010) Yong Yi et al.   PLoS One

Mechanisms of Diarrhea in Inflammatory Bowel Diseases

(2009) Henry J. Binder   Annals of the New York Academy of Sciences

Escherichia coli genes affecting recipient ability in plasmid conjugation: Are there any?

(2009) Daniel Pérez-Mendoza et al.   BMC GENOMICS

Role of Periplasmic Chaperones and BamA (YaeT/Omp85) in Folding and Secretion of Intimin from Enteropathogenic Escherichia coli Strains

(2009) G. Bodelon et al.   JOURNAL OF BACTERIOLOGY

Attaching effacing Escherichia coli and paradigms of Tir-triggered actin polymerization: getting off the pedestal

(2008) Gad Frankel et al.   CELLULAR MICROBIOLOGY

Type IV secretion systems: tools of bacterial horizontal gene transfer and virulence

(2008) Mario Juhas et al.   CELLULAR MICROBIOLOGY

Transient Shielding of Intimin and the Type III Secretion System of Enterohemorrhagic and Enteropathogenic Escherichia coli by a Group 4 Capsule

(2008) Y. Shifrin et al.   JOURNAL OF BACTERIOLOGY

Why is entry exclusion an essential feature of conjugative plasmids?

(2008) M. Pilar Garcillán-Barcia et al.   PLASMID