Systematic engineering pinpoints a versatile strategy for the expression of functional cytochrome P450 enzymes in Escherichia coli cell factories

Efficient heterologous expression of cytochrome P450 enzymes in microorganisms for the biosynthesis of natural products

(2022) Baodong Hu et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Engineering and optimization of the 2‐phenylethylglucosinolate production in Nicotiana benthamiana by combining biosynthetic genes from Barbarea vulgaris and Arabidopsis thaliana

(2021) Cuiwei Wang et al.   PLANT JOURNAL

Rational design strategies for functional reconstitution of plant cytochrome P450s in microbial systems

(2021) Anqi Zhou et al.   CURRENT OPINION IN PLANT BIOLOGY

Influence of Transmembrane Helix Mutations on Cytochrome P450-Membrane Interactions and Function

(2019) Ghulam Mustafa et al.   BIOPHYSICAL JOURNAL

De novo production of benzyl glucosinolate in Escherichia coli

(2019) Annette Petersen et al.   METABOLIC ENGINEERING

Recombinant production of eukaryotic cytochrome P450s in microbial cell factories

(2018) Johanna Hausjell et al.   BIOSCIENCE REPORTS

An expression tag toolbox for microbial production of membrane bound plant cytochromes P450

(2016) Dario Vazquez-Albacete et al.   BIOTECHNOLOGY AND BIOENGINEERING

Overcoming heterologous protein interdependency to optimize P450-mediated Taxol precursor synthesis inEscherichia coli

(2016) Bradley Walters Biggs et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Total biosynthesis of opiates by stepwise fermentation using engineered Escherichia coli

(2016) Akira Nakagawa et al.   Nature Communications

Engineering of Secondary Metabolism

(2015) Sarah E. O'Connor   Annual Review of Genetics

Sequence modifications and heterologous expression of eukaryotic cytochromes P450 in Escherichia coli

(2015) Hirofumi Ichinose et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Complete biosynthesis of opioids in yeast

(2015) S. Galanie et al.   SCIENCE

Accurate DNA Assembly and Genome Engineering with Optimized Uracil Excision Cloning

(2015) Ana Mafalda Cavaleiro et al.   ACS Synthetic Biology

Biosynthetic Pathway for the Cyanide-Free Production of Phenylacetonitrile in Escherichia coli by Utilizing Plant Cytochrome P450 79A2 and Bacterial Aldoxime Dehydratase

(2014) Yuta Miki et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Semi-synthetic artemisinin: a model for the use of synthetic biology in pharmaceutical development

(2014) Chris J. Paddon et al.   NATURE REVIEWS MICROBIOLOGY

A fragmentation-based method for the differentiation of glutathione conjugates by high-resolution mass spectrometry with electrospray ionization

(2013) Cen Xie et al.   ANALYTICA CHIMICA ACTA

Soluble and membrane-bound Drosophila melanogaster CYP6G1 expressed in Escherichia coli: Purification, activity, and binding properties toward multiple pesticides

(2013) Matthew J. Cheesman et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

Optimizations to achieve high-level expression of cytochrome P450 proteins using Escherichia coli expression systems

(2013) Susan Zelasko et al.   PROTEIN EXPRESSION AND PURIFICATION

Plant natural products: history, limitations and the potential of cambial meristematic cells

(2012) Byung-Wook Yun et al.   Biotechnology & Genetic Engineering Reviews

Microbial production of indolylglucosinolate through engineering of a multi-gene pathway in a versatile yeast expression platform

(2012) Michael Dalgaard Mikkelsen et al.   METABOLIC ENGINEERING

Cytosolic γ-Glutamyl Peptidases Process Glutathione Conjugates in the Biosynthesis of Glucosinolates and Camalexin inArabidopsis

(2011) Fernando Geu-Flores et al.   PLANT CELL

Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli

(2010) P. K. Ajikumar et al.   SCIENCE

Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli

(2009) Bryson D Bennett et al.   Nature Chemical Biology