An expression tag toolbox for microbial production of membrane bound plant cytochromes P450

The TOPCONS web server for consensus prediction of membrane protein topology and signal peptides

(2015) Konstantinos D. Tsirigos et al.   NUCLEIC ACIDS RESEARCH

Accurate DNA Assembly and Genome Engineering with Optimized Uracil Excision Cloning

(2015) Ana Mafalda Cavaleiro et al.   ACS Synthetic Biology

Enhanced Protein Production in Escherichia coli by Optimization of Cloning Scars at the Vector–Coding Sequence Junction

(2015) Kiavash Mirzadeh et al.   ACS Synthetic Biology

Identification of Putative Substrates for the Periplasmic Chaperone YfgM inEscherichia coliUsing Quantitative Proteomics

(2014) Hansjörg Götzke et al.   MOLECULAR & CELLULAR PROTEOMICS

Production of Bioactive Diterpenoids in the Euphorbiaceae Depends on Evolutionarily Conserved Gene Clusters

(2014) A. J. King et al.   PLANT CELL

Improved production of membrane proteins inEscherichia coliby selective codon substitutions

(2013) Morten H.H. Nørholm et al.   FEBS LETTERS

Cytochrome P450-encoding genes from theHeliconiusgenome as candidates for cyanogenesis

(2013) R. Chauhan et al.   INSECT MOLECULAR BIOLOGY

Novel strategy for production of aggregation-prone proteins and lytic enzymes in Escherichia coli based on an anchored periplasmic expression system

(2013) Jae Hyung Lee et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Geraniol hydroxylase and hydroxygeraniol oxidase activities of the CYP76 family of cytochrome P450 enzymes and potential for engineering the early steps of the (seco)iridoid pathway

(2013) René Höfer et al.   METABOLIC ENGINEERING

Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels

(2013) Susan Schlegel et al.   Microbial Cell Factories

Using Haloarcula marismortui Bacteriorhodopsin as a Fusion Tag for Enhancing and Visible Expression of Integral Membrane Proteins in Escherichia coli

(2013) Min-Feng Hsu et al.   PLoS One

Application of HaloTag technology to expression and purification of cannabinoid receptor CB2

(2013) Silvia Locatelli-Hoops et al.   PROTEIN EXPRESSION AND PURIFICATION

14-Step Synthesis of (+)-Ingenol from (+)-3-Carene

(2013) L. Jorgensen et al.   SCIENCE

Diversity of P450 enzymes in the biosynthesis of natural products

(2012) Larissa M. Podust et al.   NATURAL PRODUCT REPORTS

Consequences of the Overexpression of a Eukaryotic Membrane Protein, the Human KDEL Receptor, in Escherichia coli

(2011) Mirjam M. Klepsch et al.   JOURNAL OF MOLECULAR BIOLOGY

Characterization of DNA polymerase β from Danio rerio by overexpression in E. coli using the in vivo/in vitro compatible pIVEX plasmid

(2011) Tomomi Ishido et al.   Microbial Cell Factories

Comparison of two codon optimization strategies to enhance recombinant protein production in Escherichia coli

(2011) Hugo G Menzella   Microbial Cell Factories

SignalP 4.0: discriminating signal peptides from transmembrane regions

(2011) Thomas Nordahl Petersen et al.   NATURE METHODS

Conformational changes of the NADPH-dependent cytochrome P450 reductase in the course of electron transfer to cytochromes P450

(2010) Tomas Laursen et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli

(2010) P. K. Ajikumar et al.   SCIENCE

flowCore: a Bioconductor package for high throughput flow cytometry

(2009) Florian Hahne et al.   BMC BIOINFORMATICS

Coding-Sequence Determinants of Gene Expression in Escherichia coli

(2009) G. Kudla et al.   SCIENCE

Revolutionizing membrane protein overexpression in bacteria

(2009) Susan Schlegel et al.   Microbial Biotechnology

Using flowViz to visualize flow cytometry data

(2008) D. Sarkar et al.   BIOINFORMATICS

Tuning Escherichia coli for membrane protein overexpression

(2008) S. Wagner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA