Recombinant production of eukaryotic cytochrome P450s in microbial cell factories

Impact on CYP19A1 activity by mutations in NADPH cytochrome P450 oxidoreductase

(2017) Christa E. Flück et al.   JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY

An expression tag toolbox for microbial production of membrane bound plant cytochromes P450

(2016) Dario Vazquez-Albacete et al.   BIOTECHNOLOGY AND BIOENGINEERING

Applications of microbial cytochrome P450 enzymes in biotechnology and synthetic biology

(2016) Hazel M Girvan et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Heterologous expression of fungal cytochromes P450 (CYP5136A1 and CYP5136A3) from the white-rot basidiomycete Phanerochaete chrysosporium : Functionalization with cytochrome b 5 in Escherichia coli

(2016) Mayumi Hatakeyama et al.   ENZYME AND MICROBIAL TECHNOLOGY

Co-expression of active human cytochrome P450 1A2 and cytochrome P450 reductase on the cell surface of Escherichia coli

(2016) Paul Quehl et al.   Microbial Cell Factories

Recent Structural Insights into Cytochrome P450 Function

(2016) F. Peter Guengerich et al.   TRENDS IN PHARMACOLOGICAL SCIENCES

Functional characterization of zebrafish cytochrome P450 1 family proteins expressed in yeast

(2015) John J. Stegeman et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Expression in yeast, new substrates, and construction of a first 3D model of human orphan cytochrome P450 2U1: Interpretation of substrate hydroxylation regioselectivity from docking studies

(2015) Lionel Ducassou et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Sequence modifications and heterologous expression of eukaryotic cytochromes P450 in Escherichia coli

(2015) Hirofumi Ichinose et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Characterization of the Ala62Pro polymorphic variant of human cytochrome P450 1A1 using recombinant protein expression

(2015) Seung Heon Lee et al.   TOXICOLOGY AND APPLIED PHARMACOLOGY

Whole-cell biotransformation with recombinant cytochrome P450 for the selective oxidation of Grundmann’s ketone

(2014) Alba Hernández-Martín et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Heterologous expression and functional characterization of the NADPH-cytochrome P450 reductase from Capsicum annuum

(2014) Ga-Young Lee et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY

A Novel Methodology for Enhanced and Consistent Heterologous Expression of Unmodified Human Cytochrome P450 1B1 (CYP1B1)

(2014) Muneeb A. Faiq et al.   PLoS One

Architecture of a single membrane spanning cytochrome P450 suggests constraints that orient the catalytic domain relative to a bilayer

(2014) B. C. Monk et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Recombinant protein expression in Escherichia coli: advances and challenges

(2014) Germán L. Rosano et al.   Frontiers in Microbiology

High-level heterologous expression of fungal cytochrome P450s in Escherichia coli

(2013) Hirofumi Ichinose et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

The central role of mosquito cytochrome P450 CYP6Zs in insecticide detoxification revealed by functional expression and structural modelling

(2013) Alexia Chandor-Proust et al.   BIOCHEMICAL JOURNAL

Soluble and membrane-bound Drosophila melanogaster CYP6G1 expressed in Escherichia coli: Purification, activity, and binding properties toward multiple pesticides

(2013) Matthew J. Cheesman et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

Production of ortho-hydroxydaidzein derivatives by a recombinant strain of Pichia pastoris harboring a cytochrome P450 fusion gene

(2013) Te-Sheng Chang et al.   PROCESS BIOCHEMISTRY

Co-expression of human cytochrome b5 increases expression of cytochrome P450 3A4 in Escherichia coli by stabilizing mRNA

(2013) Mi-Sook Dong et al.   PROTEIN EXPRESSION AND PURIFICATION

Optimizations to achieve high-level expression of cytochrome P450 proteins using Escherichia coli expression systems

(2013) Susan Zelasko et al.   PROTEIN EXPRESSION AND PURIFICATION

Strategies for the Production of Recombinant Protein in Escherichia coli

(2013) Gopal Jee Gopal et al.   PROTEIN JOURNAL

Construction and Application of a Functional Library of Cytochrome P450 Monooxygenases from the Filamentous Fungus Aspergillus oryzae

(2011) K. H. M. Nazmul Hussain Nazir et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Heterologous Expression of Human Cytochromes P450 2D6 and CYP3A4 in Escherichia coli and Their Functional Characterization

(2011) Yan Pan et al.   PROTEIN JOURNAL

Genome-to-function characterization of novel fungal P450 monooxygenases oxidizing polycyclic aromatic hydrocarbons (PAHs)

(2010) Khajamohiddin Syed et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Improved expression of recombinant cytochrome P450 monooxygenase in Escherichia coli for asymmetric oxidation of sulfides

(2010) Jian-Dong Zhang et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Cloning, Expression and Functional Characterization of Cytochrome P450 3A37 from Turkey Liver with High Aflatoxin B1Epoxidation Activity†

(2010) Sumit Rawal et al.   CHEMICAL RESEARCH IN TOXICOLOGY

Functional characterization of eight human cytochrome P450 1A2 gene variants by recombinant protein expression

(2010) B Brito Palma et al.   PHARMACOGENOMICS JOURNAL

Co-expression of ferrochelatase allows for complete heme incorporation into recombinant proteins produced in E. coli

(2010) Jawahar Sudhamsu et al.   PROTEIN EXPRESSION AND PURIFICATION

Human cytochrome P450 4F11: Heterologous expression in bacteria, purification, and characterization of catalytic function

(2009) Zhongmei Tang et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Tuning Escherichia coli for membrane protein overexpression

(2008) S. Wagner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA