Strategies for precise gene edits in mammalian cells

Bacterial Retrons Enable Precise Gene Editing in Human Cells

(2022) Bin Zhao et al.   CRISPR Journal

Efficient targeted insertion of large DNA fragments without DNA donors

(2022) Jinlin Wang et al.   NATURE METHODS

Enhancement of prime editing via xrRNA motif-joined pegRNA

(2022) Guiquan Zhang et al.   Nature Communications

Designing and executing prime editing experiments in mammalian cells

(2022) Jordan L. Doman et al.   Nature Protocols

Mechanistic details of CRISPR-associated transposon recruitment and integration revealed by cryo-EM

(2022) Jung-Un Park et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Multiplex base editing to convert TAG into TAA codons in the human genome

(2022) Yuting Chen et al.   Nature Communications

CRISPR-assisted transposition: TnsC finds (and threads) the needle in the haystack

(2022) Amer A. Hossain et al.   MOLECULAR CELL

Selective TnsC recruitment enhances the fidelity of RNA-guided transposition

(2022) Florian T. Hoffmann et al.   NATURE

Structure of the TnsB transposase-DNA complex of type V-K CRISPR-associated transposon

(2022) Francisco Tenjo-Castaño et al.   Nature Communications

Structural basis for the assembly of the type V CRISPR-associated transposon complex

(2022) Michael Schmitz et al.   CELL

Structures of the holo CRISPR RNA-guided transposon integration complex

(2022) Jung-Un Park et al.   NATURE

Decorating chromatin for enhanced genome editing using CRISPR-Cas9

(2022) Evelyn Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Cas9–transcription factor fusion protein enhances homology-directed repair efficiency

(2021) Guoling Li et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

DNA Repair Pathway Choices in CRISPR-Cas9-Mediated Genome Editing

(2021) Chaoyou Xue et al.   TRENDS IN GENETICS

Improved prime editors enable pathogenic allele correction and cancer modelling in adult mice

(2021) Pengpeng Liu et al.   Nature Communications

Homology-based repair induced by CRISPR-Cas nucleases in mammalian embryo genome editing

(2021) Xiya Zhang et al.   Protein & Cell

A Novel Set of Cas9 Fusion Proteins to Stimulate Homologous Recombination: Cas9-HRs

(2021) Chris R. Hackley   CRISPR Journal

Enhancing prime editing by Csy4-mediated processing of pegRNA

(2021) Yao Liu et al.   CELL RESEARCH

Enhanced prime editing systems by manipulating cellular determinants of editing outcomes

(2021) Peter J. Chen et al.   CELL

Reconsidering pathway choice: a sequential model of mammalian DNA double-strand break pathway decisions

(2021) Tanya T Paull   CURRENT OPINION IN GENETICS & DEVELOPMENT

Transposon-associated TnpB is a programmable RNA-guided DNA endonuclease

(2021) Tautvydas Karvelis et al.   NATURE

Deletion and replacement of long genomic sequences using prime editing

(2021) Tingting Jiang et al.   NATURE BIOTECHNOLOGY

Engineered pegRNAs improve prime editing efficiency

(2021) James W. Nelson et al.   NATURE BIOTECHNOLOGY

Increasing the efficiency and precision of prime editing with guide RNA pairs

(2021) Yuan Zhuang et al.   Nature Chemical Biology

Precise genome editing across kingdoms of life using retron-derived DNA

(2021) Santiago C. Lopez et al.   Nature Chemical Biology

Rapid genome editing by CRISPR-Cas9-POLD3 fusion

(2021) Ganna Reint et al.   eLife

DNA repair protein RAD51 enhances the CRISPR/Cas9-mediated knock-in efficiency in brain neurons

(2020) Taiga Kurihara et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Expanding the genome-targeting scope and the site selectivity of high-precision base editors

(2020) Junjie Tan et al.   Nature Communications

Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair

(2020) Beeke Wienert et al.   Nature Communications

Improving the efficiency of precise genome editing with site-specific Cas9-oligonucleotide conjugates

(2020) Xinyu Ling et al.   Science Advances

Role of RS-1 derivatives in homology-directed repair at the human genome ATG5 locus

(2020) In-Sook Jeon et al.   ARCHIVES OF PHARMACAL RESEARCH

Increasing CRISPR/Cas9-mediated homology-directed DNA repair by histone deacetylase inhibitors

(2020) Guoling Li et al.   INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY

Suppression of SHROOM1 Improves In Vitro and In Vivo Gene Integration by Promoting Homology-Directed Repair

(2020) Zhihua Zhao et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells

(2020) Ibrahim C. Kurt et al.   NATURE BIOTECHNOLOGY

Efficient gene editing of human long-term hematopoietic stem cells validated by clonal tracking

(2020) Samuele Ferrari et al.   NATURE BIOTECHNOLOGY

Very fast CRISPR on demand

(2020) Yang Liu et al.   SCIENCE

Controlled Cycling and Quiescence Enables Efficient HDR in Engraftment-Enriched Adult Hematopoietic Stem and Progenitor Cells

(2020) Jiyung J. Shin et al.   Cell Reports

CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genome engineering

(2020) Phuc Leo H. Vo et al.   NATURE BIOTECHNOLOGY

CRISPR-Cas9 Gene Editing for Sickle Cell Disease and β-Thalassemia

(2020) Haydar Frangoul et al.   NEW ENGLAND JOURNAL OF MEDICINE

MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits

(2020) Linyuan Ma et al.   Nature Communications

Highly efficient therapeutic gene editing of human hematopoietic stem cells

(2019) Yuxuan Wu et al.   NATURE MEDICINE

Precise Gene Editing Preserves Hematopoietic Stem Cell Function following Transient p53-Mediated DNA Damage Response

(2019) Giulia Schiroli et al.   Cell Stem Cell

Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors

(2019) Tony P. Huang et al.   NATURE BIOTECHNOLOGY

Targeted homology-directed repair in blood stem and progenitor cells with CRISPR nanoformulations

(2019) Reza Shahbazi et al.   NATURE MATERIALS

Enhancement of Precise Gene Editing by the Association of Cas9 With Homologous Recombination Factors

(2019) Ngoc-Tung Tran et al.   Frontiers in Genetics

Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integration

(2019) Sanne E. Klompe et al.   NATURE

DNA double-strand break repair-pathway choice in somatic mammalian cells

(2019) Ralph Scully et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Development of hRad51–Cas9 nickase fusions that mediate HDR without double-stranded breaks

(2019) Holly A. Rees et al.   Nature Communications

Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant

(2019) Tarun S. Nambiar et al.   Nature Communications

CRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites

(2019) Rajeswari Jayavaradhan et al.   Nature Communications

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

Retrons and their applications in genome engineering

(2019) Anna J Simon et al.   NUCLEIC ACIDS RESEARCH

Recruitment of DNA Repair MRN Complex by Intrinsically Disordered Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated Genome Editing

(2019) Nina Reuven et al.   Biomolecules

BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity

(2018) Wen Jiang et al.   CELL RESEARCH

A Versatile Tool for the Quantification of CRISPR/Cas9-Induced Genome Editing Events in Human Hematopoietic Cell Lines and Hematopoietic Stem/Progenitor Cells

(2018) Rajeswari Jayavaradhan et al.   JOURNAL OF MOLECULAR BIOLOGY

Enhanced Bacterial Immunity and Mammalian Genome Editing via RNA-Polymerase-Mediated Dislodging of Cas9 from Double-Strand DNA Breaks

(2018) Ryan Clarke et al.   MOLECULAR CELL

Base editing with a Cpf1–cytidine deaminase fusion

(2018) Xiaosa Li et al.   NATURE BIOTECHNOLOGY

Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos

(2018) Bin Gu et al.   NATURE BIOTECHNOLOGY

Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction

(2018) Luke W Koblan et al.   NATURE BIOTECHNOLOGY

A multi-landing pad DNA integration platform for mammalian cell engineering

(2018) Leonid Gaidukov et al.   NUCLEIC ACIDS RESEARCH

CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair

(2018) M. Charpentier et al.   Nature Communications

Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair

(2018) Natasa Savic et al.   eLife

Efficient Knock-in of a Point Mutation in Porcine Fibroblasts Using the CRISPR/Cas9-GMNN Fusion Gene

(2018) Max Gerlach et al.   Genes

Priming Human Repopulating Hematopoietic Stem and Progenitor Cells for Cas9/sgRNA Gene Targeting

(2018) Carsten T. Charlesworth et al.   Molecular Therapy-Nucleic Acids

Functional Genetic Variants Revealed by Massively Parallel Precise Genome Editing

(2018) Eilon Sharon et al.   CELL

Improving Gene Editing Outcomes in Human Hematopoietic Stem and Progenitor Cells by Temporal Control of DNA Repair

(2018) Anastasia Lomova et al.   STEM CELLS

Enhancing CRISPR/Cas9-mediated homology-directed repair in mammalian cells by expressing Saccharomyces cerevisiae Rad52

(2017) Simin Shao et al.   INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY

CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons

(2017) Pierre Billon et al.   MOLECULAR CELL

Precise Editing of a Target Base in the Rice Genome Using a Modified CRISPR/Cas9 System

(2017) Yuming Lu et al.   Molecular Plant

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

(2017) Nicole M. Gaudelli et al.   NATURE

Inhibition of 53BP1 favors homology-dependent DNA repair and increases CRISPR–Cas9 genome-editing efficiency

(2017) Marella D Canny et al.   NATURE BIOTECHNOLOGY

Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion

(2017) Yuan Zong et al.   NATURE BIOTECHNOLOGY

CRISPR-STOP: gene silencing through base-editing-induced nonsense mutations

(2017) Cem Kuscu et al.   NATURE METHODS

Recruitment of CRISPR-Cas systems by Tn7-like transposons

(2017) Joseph E. Peters et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing

(2017) Jared Carlson-Stevermer et al.   Nature Communications

Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system

(2017) Yihan Zhang et al.   Nature Communications

Characterization of the interplay between DNA repair and CRISPR/Cas9-induced DNA lesions at an endogenous locus

(2017) Anne Bothmer et al.   Nature Communications

Improving the DNA specificity and applicability of base editing through protein engineering and protein delivery

(2017) Holly A. Rees et al.   Nature Communications

Highly efficient and precise base editing in discarded human tripronuclear embryos

(2017) Guanglei Li et al.   Protein & Cell

Correction of β-thalassemia mutant by base editor in human embryos

(2017) Puping Liang et al.   Protein & Cell

Synthetically modified guide RNA and donor DNA are a versatile platform for CRISPR-Cas9 engineering

(2017) Kunwoo Lee et al.   eLife

Ectopic expression of RAD52 and dn53BP1 improves homology-directed repair during CRISPR–Cas9 genome editing

(2017) Bruna S. Paulsen et al.   Nature Biomedical Engineering

Precise genome-wide base editing by the CRISPR Nickase system in yeast

(2017) Atsushi Satomura et al.   Scientific Reports

Deep mutational scanning of S. pyogenes Cas9 reveals important functional domains

(2017) Jeffrey M. Spencer et al.   Scientific Reports

Efficient introduction of specific homozygous and heterozygous mutations using CRISPR/Cas9

(2016) Dominik Paquet et al.   NATURE

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

Enhancing homology-directed genome editing by catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA

(2016) Christopher D Richardson et al.   NATURE BIOTECHNOLOGY

Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems

(2016) K. Nishida et al.   SCIENCE

Repair Pathway Choices and Consequences at the Double-Strand Break

(2016) Raphael Ceccaldi et al.   TRENDS IN CELL BIOLOGY

RS-1 enhances CRISPR/Cas9- and TALEN-mediated knock-in efficiency

(2016) Jun Song et al.   Nature Communications

Two Distinct Pathways Support Gene Correction by Single-Stranded Donors at DNA Nicks

(2016) Luther Davis et al.   Cell Reports

Post-translational Regulation of Cas9 during G1 Enhances Homology-Directed Repair

(2016) Tony Gutschner et al.   Cell Reports

A Cas9 Variant for Efficient Generation of Indel-Free Knockin or Gene-Corrected Human Pluripotent Stem Cells

(2016) Sara E. Howden et al.   Stem Cell Reports

Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining

(2015) Takeshi Maruyama et al.   NATURE BIOTECHNOLOGY

Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells

(2015) Van Trung Chu et al.   NATURE BIOTECHNOLOGY

Nuclear domain ‘knock-in’ screen for the evaluation and identification of small molecule enhancers of CRISPR-based genome editing

(2015) Jordan Pinder et al.   NUCLEIC ACIDS RESEARCH

Pharmacological inhibition of DNA-PK stimulates Cas9-mediated genome editing

(2015) Francis Robert et al.   Genome Medicine

Nonhomologous end joining: A good solution for bad ends

(2014) Crystal A. Waters et al.   DNA REPAIR

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

(2014) S. Kim et al.   GENOME RESEARCH

Aptamer-guided gene targeting in yeast and human cells

(2014) Patrick Ruff et al.   NUCLEIC ACIDS RESEARCH

Homology-directed repair of DNA nicks via pathways distinct from canonical double-strand break repair

(2014) L. Davis et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Is Non-Homologous End-Joining Really an Inherently Error-Prone Process?

(2014) Mireille Bétermier et al.   PLoS Genetics

Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

(2014) Steven Lin et al.   eLife

Fusion protein linkers: Property, design and functionality

(2012) Xiaoying Chen et al.   ADVANCED DRUG DELIVERY REVIEWS

Playing the End Game: DNA Double-Strand Break Repair Pathway Choice

(2012) J. Ross Chapman et al.   MOLECULAR CELL

Bi-allelic gene targeting in mouse embryonic stem cells

(2011) Peri H. Tate et al.   METHODS

Regulation of Homologous Recombination in Eukaryotes

(2010) Wolf-Dietrich Heyer et al.   Annual Review of Genetics

DNA Repair in Mammalian Cells

(2009) C. Kunz et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Ku and DNA-dependent Protein Kinase Dynamic Conformations and Assembly Regulate DNA Binding and the Initial Non-homologous End Joining Complex

(2009) Michal Hammel et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

DNA Damage, Aging, and Cancer

(2009) Jan H.J. Hoeijmakers   NEW ENGLAND JOURNAL OF MEDICINE

Comparison of nonhomologous end joining and homologous recombination in human cells

(2008) Z MAO et al.   DNA REPAIR

A protocol for removal of antibiotic resistance cassettes from human embryonic stem cells genetically modified by homologous recombination or transgenesis

(2008) Richard P Davis et al.   Nature Protocols