Designing and executing prime editing experiments in mammalian cells
Published 2022 View Full Article
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Title
Designing and executing prime editing experiments in mammalian cells
Authors
Keywords
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Journal
Nature Protocols
Volume -, Issue -, Pages -
Publisher
Springer Science and Business Media LLC
Online
2022-08-09
DOI
10.1038/s41596-022-00724-4
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- (2022) Steven Erwood et al. NATURE BIOTECHNOLOGY
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- (2022) Desirée Böck et al. Science Translational Medicine
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- (2022) Guiquan Zhang et al. Nature Communications
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- (2022) Junhong Choi et al. NATURE
- Bi-PE: bi-directional priming improves CRISPR/Cas9 prime editing in mammalian cells
- (2022) Rui Tao et al. NUCLEIC ACIDS RESEARCH
- In vivo base editing rescues Hutchinson–Gilford progeria syndrome in mice
- (2021) Luke W. Koblan et al. NATURE
- Quantitative evaluation of chromosomal rearrangements in gene-edited human stem cells by CAST-Seq
- (2021) Giandomenico Turchiano et al. Cell Stem Cell
- PrimeDesign software for rapid and simplified design of prime editing guide RNAs
- (2021) Jonathan Y. Hsu et al. Nature Communications
- Programmable C:G to G:C genome editing with CRISPR-Cas9-directed base excision repair proteins
- (2021) Liwei Chen et al. Nature Communications
- Adenine base editing and prime editing of chemically derived hepatic progenitors rescue genetic liver disease
- (2021) Yohan Kim et al. Cell Stem Cell
- CRISPR prime editing with ribonucleoprotein complexes in zebrafish and primary human cells
- (2021) Karl Petri et al. NATURE BIOTECHNOLOGY
- High-efficiency prime editing with optimized, paired pegRNAs in plants
- (2021) Qiupeng Lin et al. NATURE BIOTECHNOLOGY
- Genome-wide specificity of prime editors in plants
- (2021) Shuai Jin et al. NATURE BIOTECHNOLOGY
- Chromothripsis as an on-target consequence of CRISPR–Cas9 genome editing
- (2021) Mitchell L. Leibowitz et al. NATURE GENETICS
- Generation and characterization of human induced pluripotent stem cells (iPSCs) from three male and three female patients with CDKL5 Deficiency Disorder (CDD)
- (2021) Pin-Fang Chen et al. Stem Cell Research
- Improved prime editors enable pathogenic allele correction and cancer modelling in adult mice
- (2021) Pengpeng Liu et al. Nature Communications
- Enhancing prime editing by Csy4-mediated processing of pegRNA
- (2021) Yao Liu et al. CELL RESEARCH
- In vivo somatic cell base editing and prime editing
- (2021) Gregory A. Newby et al. MOLECULAR THERAPY
- Dual-AAV delivering split prime editor system for in vivo genome editing
- (2021) Shengyao Zhi et al. MOLECULAR THERAPY
- Base editing of haematopoietic stem cells rescues sickle cell disease in mice
- (2021) Gregory A. Newby et al. NATURE
- Efficient C•G-to-G•C base editors developed using CRISPRi screens, target-library analysis, and machine learning
- (2021) Luke W. Koblan et al. NATURE BIOTECHNOLOGY
- CRISPR-Cas9 In Vivo Gene Editing for Transthyretin Amyloidosis
- (2021) Julian D. Gillmore et al. NEW ENGLAND JOURNAL OF MEDICINE
- pegIT - a web-based design tool for prime editing
- (2021) Mads Valdemar Anderson et al. NUCLEIC ACIDS RESEARCH
- Optimization of C-to-G base editors with sequence context preference predictable by machine learning methods
- (2021) Tanglong Yuan et al. Nature Communications
- Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain
- (2021) Myungjae Song et al. Nature Communications
- Modeling a cataract disorder in mice with prime editing
- (2021) Jianxiang Lin et al. Molecular Therapy-Nucleic Acids
- Efficient and precise generation of Tay–Sachs disease model in rabbit by prime editing system
- (2021) Yuqiang Qian et al. Cell Discovery
- Application of prime editing to the correction of mutations and phenotypes in adult mice with liver and eye diseases
- (2021) Hyewon Jang et al. Nature Biomedical Engineering
- Enhanced prime editing systems by manipulating cellular determinants of editing outcomes
- (2021) Peter J. Chen et al. CELL
- Mapping the genetic landscape of DNA double-strand break repair
- (2021) Jeffrey A. Hussmann et al. CELL
- Deletion and replacement of long genomic sequences using prime editing
- (2021) Tingting Jiang et al. NATURE BIOTECHNOLOGY
- Engineered pegRNAs improve prime editing efficiency
- (2021) James W. Nelson et al. NATURE BIOTECHNOLOGY
- Precise genomic deletions using paired prime editing
- (2021) Junhong Choi et al. NATURE BIOTECHNOLOGY
- Programmable deletion, replacement, integration and inversion of large DNA sequences with twin prime editing
- (2021) Andrew V. Anzalone et al. NATURE BIOTECHNOLOGY
- Increasing the efficiency and precision of prime editing with guide RNA pairs
- (2021) Yuan Zhuang et al. Nature Chemical Biology
- Comprehensive analysis of prime editing outcomes in human embryonic stem cells
- (2021) Omer Habib et al. NUCLEIC ACIDS RESEARCH
- CRISPR-engineered T cells in patients with refractory cancer
- (2020) Edward A. Stadtmauer et al. SCIENCE
- Bi-directional differentiation of single bronchioalveolar stem cells during lung repair
- (2020) Kuo Liu et al. Cell Discovery
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- (2020) Qiupeng Lin et al. NATURE BIOTECHNOLOGY
- Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
- (2020) Michelle F. Richter et al. NATURE BIOTECHNOLOGY
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- (2020) Oana M. Enache et al. NATURE GENETICS
- Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
- (2020) Andrew V. Anzalone et al. NATURE BIOTECHNOLOGY
- CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells
- (2020) Ibrahim C. Kurt et al. NATURE BIOTECHNOLOGY
- Glycosylase base editors enable C-to-A and C-to-G base changes
- (2020) Dongdong Zhao et al. NATURE BIOTECHNOLOGY
- Predicting the efficiency of prime editing guide RNAs in human cells
- (2020) Hui Kwon Kim et al. NATURE BIOTECHNOLOGY
- Unbiased investigation of specificities of prime editing systems in human cells
- (2020) Do Yon Kim et al. NUCLEIC ACIDS RESEARCH
- Prime editing for functional repair in patient-derived disease models
- (2020) Imre F. Schene et al. Nature Communications
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- (2020) Yuning Song et al. Molecular Therapy-Nucleic Acids
- A web tool for the design of prime-editing guide RNAs
- (2020) Ryan D. Chow et al. Nature Biomedical Engineering
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- (2020) Michael V. Zuccaro et al. CELL
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- (2020) Haydar Frangoul et al. NEW ENGLAND JOURNAL OF MEDICINE
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- (2019) Andrew V. Anzalone et al. NATURE
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- (2019) Beau R. Webber et al. Nature Communications
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- (2018) Georgia Giannoukos et al. BMC GENOMICS
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- (2018) Michael Kosicki et al. NATURE BIOTECHNOLOGY
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- (2012) J. Ross Chapman et al. MOLECULAR CELL
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- (2012) M. Jinek et al. SCIENCE
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