Metabolic Engineering for Efficient Ketocarotenoid Accumulation in the Green Microalga Chlamydomonas reinhardtii

Simultaneous accumulation of astaxanthin and β-carotene in Chlamydomonas reinhardtii by the introduction of foreign β-carotene hydroxylase gene in response to high light stress

(2022) Kunmei Huang et al.   BIOTECHNOLOGY LETTERS

Combinatorial Engineering Enables Photoautotrophic Growth in High Cell Density Phosphite-Buffered Media to Support Engineered Chlamydomonas reinhardtii Bio-Production Concepts

(2022) Malak N. Abdallah et al.   Frontiers in Microbiology

The Spermidine Synthase Gene SPD1: A Novel Auxotrophic Marker for Chlamydomonas reinhardtii Designed by Enhanced CRISPR/Cas9 Gene Editing

(2022) Robert A. Freudenberg et al.   Cells

Sustainable production of astaxanthin in microorganisms: the past, present, and future

(2022) Xiangyu Zhu et al.   CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION

Advanced pathway engineering for phototrophic putrescine production

(2022) Robert A. Freudenberg et al.   PLANT BIOTECHNOLOGY JOURNAL

Molecular Advancements Establishing Chlamydomonas as a Host for Biotechnological Exploitation

(2022) Michael Schroda et al.   Frontiers in Plant Science

Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production

(2022) Alexander Einhaus et al.   METABOLIC ENGINEERING

Teaching an old ‘doc’ new tricks for algal biotechnology: Strategic filter use enables multi-scale fluorescent protein signal detection

(2022) Sergio Gutiérrez et al.   Frontiers in Bioengineering and Biotechnology

Lycopene β-cyclase expression influences plant physiology, development and metabolism in tobacco plants

(2021) Stella Kossler et al.   JOURNAL OF EXPERIMENTAL BOTANY

Rational Promoter Engineering Enables Robust Terpene Production in Microalgae

(2021) Alexander Einhaus et al.   ACS Synthetic Biology

Heterologous expression of cyanobacterial Orange Carotenoid Protein (OCP2) as a soluble carrier of ketocarotenoids in Chlamydomonas reinhardtii

(2021) Matteo Pivato et al.   Algal Research-Biomass Biofuels and Bioproducts

Fruit-Specific Expression of crtB, HpBHY, CrBKT and SlLCYB in a Special Tomato Landrace Triggers Hyper Production of Carotenoids in the Fruit

(2021) Yuanyuan Lin et al.   JOURNAL OF PLANT BIOLOGY

Turning a green alga red: engineering astaxanthin biosynthesis by intragenic pseudogene revival in Chlamydomonas reinhardtii

(2020) Federico Perozeni et al.   PLANT BIOTECHNOLOGY JOURNAL

Over-Expression of LcPDS, LcZDS, and LcCRTISO, Genes From Wolfberry for Carotenoid Biosynthesis, Enhanced Carotenoid Accumulation, and Salt Tolerance in Tobacco

(2020) Chen Li et al.   Frontiers in Plant Science

Microbial astaxanthin biosynthesis: recent achievements, challenges, and commercialization outlook

(2020) Congqiang Zhang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

High light intensity increases the concentrations of β-carotene and zeaxanthin in marine red macroalgae

(2020) Xiujun Xie et al.   Algal Research-Biomass Biofuels and Bioproducts

Metabolic engineering of ketocarotenoids biosynthetic pathway in Chlamydomonas reinhardtii strain CC-4102

(2020) Nam Trung Tran et al.   Scientific Reports

Introns mediate post-transcriptional enhancement of nuclear gene expression in the green microalga Chlamydomonas reinhardtii

(2020) Thomas Baier et al.   PLoS Genetics

Tailoring cyanobacteria as a new platform for highly efficient synthesis of astaxanthin

(2020) Jinjin Diao et al.   METABOLIC ENGINEERING

Synthetic conversion of leaf chloroplasts into carotenoid-rich plastids reveals mechanistic basis of natural chromoplast development

(2020) Briardo Llorente et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Photosynthesis without β-carotene

(2020) Pengqi Xu et al.   eLife

Coordinated Expression of Astaxanthin Biosynthesis Genes for Improved Astaxanthin Production in Escherichia coli

(2020) Zhongkuo Gong et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Reprogramming microorganisms for the biosynthesis of astaxanthin via metabolic engineering

(2020) Xia Wan et al.   PROGRESS IN LIPID RESEARCH

High cell density cultivation enables efficient and sustainable recombinant polyamine production in the microalga Chlamydomonas reinhardtii

(2020) Robert A. Freudenberg et al.   BIORESOURCE TECHNOLOGY

Intronserter, an advanced online tool for design of intron containing transgenes

(2019) Daniel Jaeger et al.   Algal Research-Biomass Biofuels and Bioproducts

Metabolic Engineering of Chlamydomonas reinhardtii for Enhanced β-Carotene and Lutein Production

(2019) Jayant Pralhad Rathod et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Improved Astaxanthin Production with Corynebacterium glutamicum by Application of a Membrane Fusion Protein

(2019) Nadja A. Henke et al.   Marine Drugs

Nourseothricin N-acetyl transferase (NAT), a new selectable marker for nuclear gene expression in Chlamydomonas

(2019) Xinjia Yang et al.   Plant Methods

Good News for Nuclear Transgene Expression in Chlamydomonas

(2019) Michael Schroda   Cells

Green algal hydrocarbon metabolism is an exceptional source of sustainable chemicals

(2019) Julian Wichmann et al.   CURRENT OPINION IN BIOTECHNOLOGY

Effects of the microalgae Chlamydomonas on gastrointestinal health

(2019) Francis J. Fields et al.   Journal of Functional Foods

Phototrophic production of heterologous diterpenoids and a hydroxy-functionalized derivative from Chlamydomonas reinhardtii

(2018) Kyle J. Lauersen et al.   METABOLIC ENGINEERING

Tailored carbon partitioning for phototrophic production of ( E )-α-bisabolene from the green microalga Chlamydomonas reinhardtii

(2018) Julian Wichmann et al.   METABOLIC ENGINEERING

Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii

(2018) Thomas Baier et al.   NUCLEIC ACIDS RESEARCH

A lycopene β-cyclase gene, IbLCYB2 , enhances carotenoid contents and abiotic stress tolerance in transgenic sweetpotato

(2018) Chen Kang et al.   PLANT SCIENCE

Over-accumulation of astaxanthin in Haematococcus pluvialis through chloroplast genetic engineering

(2018) Janeth I. Galarza et al.   Algal Research-Biomass Biofuels and Bioproducts

Fed-batch mixotrophic cultivation of Chlamydomonas reinhardtii for high-density cultures

(2018) Francis J. Fields et al.   Algal Research-Biomass Biofuels and Bioproducts

Astaxanthin overproduction in yeast by strain engineering and new gene target uncovering

(2018) Jin Jin et al.   Biotechnology for Biofuels

Metabolic engineering of Escherichia coli for high-level astaxanthin production with high productivity

(2018) Seon Young Park et al.   METABOLIC ENGINEERING

From Golden Rice to aSTARice: Bioengineering Astaxanthin Biosynthesis in Rice Endosperm

(2018) Qinlong Zhu et al.   Molecular Plant

Eukaryotic microalgae as hosts for light-driven heterologous isoprenoid production

(2018) Kyle J. Lauersen   PLANTA

Identification of Chlamydomonas reinhardtii endogenous genic flanking sequences for improved transgene expression

(2017) Cristina López-Paz et al.   PLANT JOURNAL

IncreasedNicotiana tabacumfitness through positive regulation of carotenoid, gibberellin and chlorophyll pathways promoted byDaucus carotalycopene β-cyclase (Dclcyb1) expression

(2016) J.C. Moreno et al.   JOURNAL OF EXPERIMENTAL BOTANY

Production of the Marine Carotenoid Astaxanthin by Metabolically Engineered Corynebacterium glutamicum

(2016) Nadja Henke et al.   Marine Drugs

Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii

(2016) Kyle J. Lauersen et al.   METABOLIC ENGINEERING

Reconstruction of the astaxanthin biosynthesis pathway in rice endosperm reveals a metabolic bottleneck at the level of endogenous β-carotene hydroxylase activity

(2016) Chao Bai et al.   TRANSGENIC RESEARCH

Astaxanthin-Producing Green Microalga Haematococcus pluvialis: From Single Cell to High Value Commercial Products

(2016) Md. Mahfuzur R. Shah et al.   Frontiers in Plant Science

Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit

(2015) Kyle J. Lauersen et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Highly efficient biosynthesis of astaxanthin in Saccharomyces cerevisiae by integration and tuning of algal crtZ and bkt

(2015) Pingping Zhou et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Enhanced astaxanthin production from microalga, Haematococcus pluvialis by two-stage perfusion culture with stepwise light irradiation

(2014) Joon Chul Park et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Molecular mechanisms of the coordination between astaxanthin and fatty acid biosynthesis inHaematococcus pluvialis(Chlorophyceae)

(2014) Guanqun Chen et al.   PLANT JOURNAL

Expression of bkt and bch genes from Haematococcus pluvialis in transgenic Chlamydomonas

(2014) KaiJing Zheng et al.   Science China-Life Sciences

Metabolic engineering of tomato for high-yield production of astaxanthin

(2013) Jun-Chao Huang et al.   METABOLIC ENGINEERING

Enhancement of carotenoid biosynthesis in the green microalga Dunaliella salina with light-emitting diodes and adaptive laboratory evolution

(2012) Weiqi Fu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

The role of the xanthophyll cycle and of lutein in photoprotection of photosystem II

(2011) Peter Jahns et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

Functional characterization of various algal carotenoid ketolases reveals that ketolating zeaxanthin efficiently is essential for high production of astaxanthin in transgenic Arabidopsis

(2011) Y.-J. Zhong et al.   JOURNAL OF EXPERIMENTAL BOTANY

A revised mineral nutrient supplement increases biomass and growth rate in Chlamydomonas reinhardtii

(2011) Janette Kropat et al.   PLANT JOURNAL

Xanthophyll cycle – a mechanism protecting plants against oxidative stress

(2011) Dariusz Latowski et al.   REDOX REPORT

Overexpression of an exogenous phytoene synthase gene in the unicellular alga Chlamydomonas reinhardtii leads to an increase in the content of carotenoids

(2010) I. Couso et al.   BIOTECHNOLOGY PROGRESS

Characterization of the  -Carotene Hydroxylase Gene DSM2 Conferring Drought and Oxidative Stress Resistance by Increasing Xanthophylls and Abscisic Acid Synthesis in Rice

(2010) H. Du et al.   PLANT PHYSIOLOGY

Overexpression of CrtR-b2 (carotene beta hydroxylase 2) from S. lycopersicum L. differentially affects xanthophyll synthesis and accumulation in transgenic tomato plants

(2010) Caterina D’Ambrosio et al.   TRANSGENIC RESEARCH

Stability Studies on Astaxanthin Extracted from Fermented Shrimp Byproducts

(2009) Roberto E. Armenta et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Generation ofChlamydomonasstrains that efficiently express nuclear transgenes

(2008) Juliane Neupert et al.   PLANT JOURNAL