Engineering a powerful green cell factory for robust photoautotrophic diterpenoid production

The Spermidine Synthase Gene SPD1: A Novel Auxotrophic Marker for Chlamydomonas reinhardtii Designed by Enhanced CRISPR/Cas9 Gene Editing

(2022) Robert A. Freudenberg et al.   Cells

Methyl Jasmonate and Methyl-β-Cyclodextrin Individually Boost Triterpenoid Biosynthesis in Chlamydomonas Reinhardtii UVM4

(2021) Audrey S. Commault et al.   Pharmaceuticals

Rational Promoter Engineering Enables Robust Terpene Production in Microalgae

(2021) Alexander Einhaus et al.   ACS Synthetic Biology

Strategies for enhancing terpenoids accumulation in microalgae

(2021) Peng-Wei Huang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Turning a green alga red: engineering astaxanthin biosynthesis by intragenic pseudogene revival in Chlamydomonas reinhardtii

(2020) Federico Perozeni et al.   PLANT BIOTECHNOLOGY JOURNAL

Introns mediate post-transcriptional enhancement of nuclear gene expression in the green microalga Chlamydomonas reinhardtii

(2020) Thomas Baier et al.   PLoS Genetics

An epigenetic gene silencing pathway selectively acting on transgenic DNA in the green alga Chlamydomonas

(2020) Juliane Neupert et al.   Nature Communications

High cell density cultivation enables efficient and sustainable recombinant polyamine production in the microalga Chlamydomonas reinhardtii

(2020) Robert A. Freudenberg et al.   BIORESOURCE TECHNOLOGY

Identifying and engineering the ideal microbial terpenoid production host

(2019) Sandra Moser et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Methyl jasmonate treatment affects the regulation of the 2-C-methyl-D-erythritol 4-phosphate pathway and early steps of the triterpenoid biosynthesis in Chlamydomonas reinhardtii

(2019) Audrey S. Commault et al.   Algal Research-Biomass Biofuels and Bioproducts

The genetic basis of grape and wine aroma

(2019) Jerry Lin et al.   Horticulture Research

Intronserter, an advanced online tool for design of intron containing transgenes

(2019) Daniel Jaeger et al.   Algal Research-Biomass Biofuels and Bioproducts

Heterologous production of labdane-type diterpenes in the green alga Chlamydomonas reinhardtii

(2019) Dimitra Papaefthimiou et al.   PHYTOCHEMISTRY

Nourseothricin N-acetyl transferase (NAT), a new selectable marker for nuclear gene expression in Chlamydomonas

(2019) Xinjia Yang et al.   Plant Methods

Good News for Nuclear Transgene Expression in Chlamydomonas

(2019) Michael Schroda   Cells

Green algal hydrocarbon metabolism is an exceptional source of sustainable chemicals

(2019) Julian Wichmann et al.   CURRENT OPINION IN BIOTECHNOLOGY

High-titer production of lathyrane diterpenoids from sugar by engineered Saccharomyces cerevisiae

(2018) Jeff Wong et al.   METABOLIC ENGINEERING

Phototrophic production of heterologous diterpenoids and a hydroxy-functionalized derivative from Chlamydomonas reinhardtii

(2018) Kyle J. Lauersen et al.   METABOLIC ENGINEERING

Tailored carbon partitioning for phototrophic production of ( E )-α-bisabolene from the green microalga Chlamydomonas reinhardtii

(2018) Julian Wichmann et al.   METABOLIC ENGINEERING

Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii

(2018) Thomas Baier et al.   NUCLEIC ACIDS RESEARCH

Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii

(2018) Pierre Crozet et al.   ACS Synthetic Biology

Eukaryotic microalgae as hosts for light-driven heterologous isoprenoid production

(2018) Kyle J. Lauersen   PLANTA

Identification of Chlamydomonas reinhardtii endogenous genic flanking sequences for improved transgene expression

(2017) Cristina López-Paz et al.   PLANT JOURNAL

Total biosynthesis of the cyclic AMP booster forskolin from Coleus forskohlii

(2017) Irini Pateraki et al.   eLife

Overcoming the plasticity of plant specialized metabolism for selective diterpene production in yeast

(2017) Codruta Ignea et al.   Scientific Reports

Engineering the methylerythritol phosphate pathway in cyanobacteria for photosynthetic isoprene production from CO2

(2016) Xiang Gao et al.   Energy & Environmental Science

Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii

(2016) Kyle J. Lauersen et al.   METABOLIC ENGINEERING

Why do plants produce so many terpenoid compounds?

(2016) Eran Pichersky et al.   NEW PHYTOLOGIST

Carnosic acid biosynthesis elucidated by a synthetic biology platform

(2016) Codruta Ignea et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Elucidation of the biosynthesis of carnosic acid and its reconstitution in yeast

(2016) Ulschan Scheler et al.   Nature Communications

Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit

(2015) Kyle J. Lauersen et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Combined metabolome and transcriptome profiling provides new insights into diterpene biosynthesis in S. pomifera glandular trichomes

(2015) Fotini A. Trikka et al.   BMC GENOMICS

Efficient diterpene production in yeast by engineering Erg20p into a geranylgeranyl diphosphate synthase

(2015) Codruta Ignea et al.   METABOLIC ENGINEERING

Sclareol production in the moss Physcomitrella patens and observations on growth and terpenoid biosynthesis

(2015) Xi-Wu Pan et al.   Plant Biotechnology Reports

Plant diterpene synthases: exploring modularity and metabolic diversity for bioengineering

(2015) Philipp Zerbe et al.   TRENDS IN BIOTECHNOLOGY

IdsA is the major geranylgeranyl pyrophosphate synthase involved in carotenogenesis inCorynebacterium glutamicum

(2014) Sabine A. E. Heider et al.   FEBS Journal

Engineering cyanobacteria for the production of a cyclic hydrocarbon fuel from CO2 and H2O

(2014) Charles Halfmann et al.   GREEN CHEMISTRY

Engineering central metabolic modules of Escherichia coli for improving β-carotene production

(2013) Jing Zhao et al.   METABOLIC ENGINEERING

Discovery and functional characterization of two diterpene synthases for sclareol biosynthesis in Salvia sclarea (L.) and their relevance for perfume manufacture

(2012) Anne Caniard et al.   BMC PLANT BIOLOGY

Toward a Biosynthetic Route to Sclareol and Amber Odorants

(2012) Michel Schalk et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Biosynthesis of isoprene in Escherichia coli via methylerythritol phosphate (MEP) pathway

(2011) Yaru Zhao et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Pathway engineering for functional isoprenoids

(2011) Norihiko Misawa   CURRENT OPINION IN BIOTECHNOLOGY

Functional effect of grapevine 1-deoxy-D-xylulose 5-phosphate synthase substitution K284N on Muscat flavour formation

(2011) Juri Battilana et al.   JOURNAL OF EXPERIMENTAL BOTANY

Phytozome: a comparative platform for green plant genomics

(2011) David M. Goodstein et al.   NUCLEIC ACIDS RESEARCH

A Modular Cloning System for Standardized Assembly of Multigene Constructs

(2011) Ernst Weber et al.   PLoS One

Photobiology of microalgae mass cultures: understanding the tools for the next green revolution

(2011) Mario R Tredici   Biofuels-UK

A candidate gene association study on muscat flavor in grapevine (Vitis vinifera L.)

(2010) Francesco Emanuelli et al.   BMC PLANT BIOLOGY

Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli

(2010) P. K. Ajikumar et al.   SCIENCE

Extracellular terpenoid hydrocarbon extraction and quantitation from the green microalgae Botryococcus braunii var. Showa

(2009) Ela Eroglu et al.   BIORESOURCE TECHNOLOGY

Unravelling the regulatory mechanisms that modulate the MEP pathway in higher plants

(2009) E. Cordoba et al.   JOURNAL OF EXPERIMENTAL BOTANY

Terpenoid biomaterials

(2008) Jörg Bohlmann et al.   PLANT JOURNAL

Generation ofChlamydomonasstrains that efficiently express nuclear transgenes

(2008) Juliane Neupert et al.   PLANT JOURNAL