The Spermidine Synthase Gene SPD1: A Novel Auxotrophic Marker for Chlamydomonas reinhardtii Designed by Enhanced CRISPR/Cas9 Gene Editing

Herbicide Selection Promotes Antibiotic Resistance in Soil Microbiomes

(2021) Hanpeng Liao et al.   MOLECULAR BIOLOGY AND EVOLUTION

Rational Promoter Engineering Enables Robust Terpene Production in Microalgae

(2021) Alexander Einhaus et al.   ACS Synthetic Biology

Turning a green alga red: engineering astaxanthin biosynthesis by intragenic pseudogene revival in Chlamydomonas reinhardtii

(2020) Federico Perozeni et al.   PLANT BIOTECHNOLOGY JOURNAL

Physiological and Molecular Responses of a Newly Evolved Auxotroph of Chlamydomonas to B12 Deprivation

(2020) Freddy Bunbury et al.   PLANT PHYSIOLOGY

TIM, a targeted insertional mutagenesis method utilizing CRISPR/Cas9 in Chlamydomonas reinhardtii

(2020) Tyler Picariello et al.   PLoS One

Site-Specific Gene Knock-Out and On-Site Heterologous Gene Overexpression in Chlamydomonas reinhardtii via a CRISPR-Cas9-Mediated Knock-in Method

(2020) Jongrae Kim et al.   Frontiers in Plant Science

Introns mediate post-transcriptional enhancement of nuclear gene expression in the green microalga Chlamydomonas reinhardtii

(2020) Thomas Baier et al.   PLoS Genetics

CRISPR based targeted genome editing of Chlamydomonas reinhardtii using programmed Cas9-gRNA ribonucleoprotein

(2020) Dhananjay Dhokane et al.   MOLECULAR BIOLOGY REPORTS

Cell Synchronization Enhances Nuclear Transformation and Genome Editing via Cas9 Enabling Homologous Recombination in Chlamydomonas reinhardtii

(2020) Max Angstenberger et al.   ACS Synthetic Biology

Achievements and challenges of genetic engineering of the model green alga Chlamydomonas reinhardtii

(2020) Nam Trung Tran et al.   Algal Research-Biomass Biofuels and Bioproducts

Genome Editing by CRISPR-Cas: A Game Change in the Genetic Manipulation of Chlamydomonas

(2020) Manel Ghribi et al.   Life-Basel

High cell density cultivation enables efficient and sustainable recombinant polyamine production in the microalga Chlamydomonas reinhardtii

(2020) Robert A. Freudenberg et al.   BIORESOURCE TECHNOLOGY

Polyamine Function in Plants: Metabolism, Regulation on Development, and Roles in Abiotic Stress Responses

(2019) Dandan Chen et al.   Frontiers in Plant Science

Temporal and metabolic overlap between lipid accumulation and programmed cell death due to nitrogen starvation in the unicellular chlorophyte Chlamydomonas reinhardtii

(2019) Santosh Sathe et al.   PHYCOLOGICAL RESEARCH

A Series of Fortunate Events: Introducing Chlamydomonas as a Reference Organism

(2019) Patrice A. Salomé et al.   PLANT CELL

Polyamines as Quality Control Metabolites Operating at the Post-Transcriptional Level

(2019) Laetitia Poidevin et al.   Plants-Basel

Intronserter, an advanced online tool for design of intron containing transgenes

(2019) Daniel Jaeger et al.   Algal Research-Biomass Biofuels and Bioproducts

Conservation of Thermospermine Synthase Activity in Vascular and Non-vascular Plants

(2019) Anna Solé-Gil et al.   Frontiers in Plant Science

A Toxicological Evaluation of Chlamydomonas reinhardtii, a Green Algae

(2018) Timothy S. Murbach et al.   INTERNATIONAL JOURNAL OF TOXICOLOGY

Phototrophic production of heterologous diterpenoids and a hydroxy-functionalized derivative from Chlamydomonas reinhardtii

(2018) Kyle J. Lauersen et al.   METABOLIC ENGINEERING

Tailored carbon partitioning for phototrophic production of ( E )-α-bisabolene from the green microalga Chlamydomonas reinhardtii

(2018) Julian Wichmann et al.   METABOLIC ENGINEERING

The algal chloroplast as a synthetic biology platform for production of therapeutic proteins

(2018) Yuliya M. Dyo et al.   MICROBIOLOGY-SGM

Next-generation biocontainment systems for engineered organisms

(2018) Jeong Wook Lee et al.   Nature Chemical Biology

Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii

(2018) Thomas Baier et al.   NUCLEIC ACIDS RESEARCH

Effect of ornithine decarboxylase and norspermidine in modulating cell division in the green alga Chlamydomonas reinhardtii

(2018) Annalisa Tassoni et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY

High-throughput system for quantifying and characterizing homologous recombination in Chlamydomonas reinhardtii

(2018) Hussam Hassan Nour-Eldin et al.   Algal Research-Biomass Biofuels and Bioproducts

One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing

(2018) Manuel Serif et al.   Nature Communications

Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii

(2018) Pierre Crozet et al.   ACS Synthetic Biology

Rapid and high efficiency transformation of Chlamydomonas reinhardtii by square-wave electroporation

(2018) Liang Wang et al.   BIOSCIENCE REPORTS

Polyamines in Microalgae: Something Borrowed, Something New

(2018) Hung-Yun Lin et al.   Marine Drugs

Engineered Fusion Proteins for Efficient Protein Secretion and Purification of a Human Growth Factor from the Green Microalga Chlamydomonas reinhardtii

(2018) Thomas Baier et al.   ACS Synthetic Biology

Antibiotic resistance: a rundown of a global crisis

(2018) Bilal Aslam et al.   Infection and Drug Resistance

eIF5A Functions Globally in Translation Elongation and Termination

(2017) Anthony P. Schuller et al.   MOLECULAR CELL

Targeting of Photoreceptor Genes inChlamydomonas reinhardtiivia Zinc-Finger Nucleases and CRISPR/Cas9

(2017) Andre Greiner et al.   PLANT CELL

Identification of Chlamydomonas reinhardtii endogenous genic flanking sequences for improved transgene expression

(2017) Cristina López-Paz et al.   PLANT JOURNAL

PCR artifact in testing for homologous recombination in genomic editing in zebrafish

(2017) Minho Won et al.   PLoS One

Efficient targeted DNA editing and replacement inChlamydomonas reinhardtiiusing Cpf1 ribonucleoproteins and single-stranded DNA

(2017) Aron Ferenczi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Towards a synthetic nuclear transcription system in green algae: Characterization of Chlamydomonas reinhardtii nuclear transcription factors and identification of targeted promoters

(2017) Melissa S. Anderson et al.   Algal Research-Biomass Biofuels and Bioproducts

Towards developing algal synthetic biology

(2016) M. A. Scaife et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Polyamines in Eukaryotes, Bacteria, and Archaea

(2016) Anthony J. Michael   JOURNAL OF BIOLOGICAL CHEMISTRY

Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii

(2016) Kyle J. Lauersen et al.   METABOLIC ENGINEERING

DNA-free two-gene knockout in Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins

(2016) Kwangryul Baek et al.   Scientific Reports

CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii

(2016) Sung-Eun Shin et al.   Scientific Reports

Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites

(2015) Jeongbin Park et al.   BIOINFORMATICS

Remaining Mysteries of Molecular Biology: The Role of Polyamines in the Cell

(2015) Leonor Miller-Fleming et al.   JOURNAL OF MOLECULAR BIOLOGY

The response of Chlamydomonas reinhardtii to nitrogen deprivation: a systems biology analysis

(2015) Jeong-Jin Park et al.   PLANT JOURNAL

Fundamental shift in vitamin B12 eco-physiology of a model alga demonstrated by experimental evolution

(2014) Katherine E Helliwell et al.   ISME Journal

PredAlgo: A New Subcellular Localization Prediction Tool Dedicated to Green Algae

(2012) Marianne Tardif et al.   MOLECULAR BIOLOGY AND EVOLUTION

A revised mineral nutrient supplement increases biomass and growth rate in Chlamydomonas reinhardtii

(2011) Janette Kropat et al.   PLANT JOURNAL

Micro-algae come of age as a platform for recombinant protein production

(2010) Elizabeth Specht et al.   BIOTECHNOLOGY LETTERS

Polyamines: molecules with regulatory functions in plant abiotic stress tolerance

(2010) Rubén Alcázar et al.   PLANTA

Functional significance of eIF5A and its hypusine modification in eukaryotes

(2009) M. H. Park et al.   AMINO ACIDS

Chlamydomonas (Chlorophyceae) colony PCR

(2009) Muqing Cao et al.   PROTOPLASMA

Is it possible to improve homologous recombination in Chlamydomonas reinhardtii?

(2008) Miroslava Slaninová et al.   BIOLOGIA

Generation ofChlamydomonasstrains that efficiently express nuclear transgenes

(2008) Juliane Neupert et al.   PLANT JOURNAL

Hypusine modification for growth is the major function of spermidine in Saccharomyces cerevisiae polyamine auxotrophs grown in limiting spermidine

(2008) M. K. Chattopadhyay et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA