Combinatorial Engineering Enables Photoautotrophic Growth in High Cell Density Phosphite-Buffered Media to Support Engineered Chlamydomonas reinhardtii Bio-Production Concepts

Heterologous expression of phosphite dehydrogenase in the chloroplast or nucleus enables phosphite utilization and genetic selection in Picochlorum spp.

(2022) Lukas R. Dahlin et al.   Algal Research-Biomass Biofuels and Bioproducts

Rational Promoter Engineering Enables Robust Terpene Production in Microalgae

(2021) Alexander Einhaus et al.   ACS Synthetic Biology

Turning a green alga red: engineering astaxanthin biosynthesis by intragenic pseudogene revival in Chlamydomonas reinhardtii

(2020) Federico Perozeni et al.   PLANT BIOTECHNOLOGY JOURNAL

High density cultivation for efficient sesquiterpenoid biosynthesis in Synechocystis sp. PCC 6803

(2020) Dennis Dienst et al.   Scientific Reports

A Phosphite Dehydrogenase Variant with Promiscuous Access to Nicotinamide Cofactor Pools Sustains Fast Phosphite-Dependent Growth of Transplastomic Chlamydomonas reinhardtii

(2020) Edoardo Cutolo et al.   Plants-Basel

Introns mediate post-transcriptional enhancement of nuclear gene expression in the green microalga Chlamydomonas reinhardtii

(2020) Thomas Baier et al.   PLoS Genetics

An epigenetic gene silencing pathway selectively acting on transgenic DNA in the green alga Chlamydomonas

(2020) Juliane Neupert et al.   Nature Communications

High cell density cultivation enables efficient and sustainable recombinant polyamine production in the microalga Chlamydomonas reinhardtii

(2020) Robert A. Freudenberg et al.   BIORESOURCE TECHNOLOGY

The phosphite oxidoreductase gene, ptxD as a bio-contained chloroplast marker and crop-protection tool for algal biotechnology using Chlamydomonas

(2019) Saowalak Changko et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Phototrophic production of heterologous diterpenoids and a hydroxy-functionalized derivative from Chlamydomonas reinhardtii

(2018) Kyle J. Lauersen et al.   METABOLIC ENGINEERING

Tailored carbon partitioning for phototrophic production of ( E )-α-bisabolene from the green microalga Chlamydomonas reinhardtii

(2018) Julian Wichmann et al.   METABOLIC ENGINEERING

The algal chloroplast as a synthetic biology platform for production of therapeutic proteins

(2018) Yuliya M. Dyo et al.   MICROBIOLOGY-SGM

Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii

(2018) Thomas Baier et al.   NUCLEIC ACIDS RESEARCH

Synthetic metabolic pathways for photobiological conversion of CO2 into hydrocarbon fuel

(2018) Ian Sofian Yunus et al.   METABOLIC ENGINEERING

Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii

(2018) Pierre Crozet et al.   ACS Synthetic Biology

Eukaryotic microalgae as hosts for light-driven heterologous isoprenoid production

(2018) Kyle J. Lauersen   PLANTA

Engineered Fusion Proteins for Efficient Protein Secretion and Purification of a Human Growth Factor from the Green Microalga Chlamydomonas reinhardtii

(2018) Thomas Baier et al.   ACS Synthetic Biology

New tools for chloroplast genetic engineering allow the synthesis of human growth hormone in the green alga Chlamydomonas reinhardtii

(2016) Thanyanan Wannathong et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii

(2016) Kyle J. Lauersen et al.   METABOLIC ENGINEERING

A novel genetic engineering platform for the effective management of biological contaminants for the production of microalgae

(2016) Maribel M. Loera-Quezada et al.   PLANT BIOTECHNOLOGY JOURNAL

Efficient expression of nuclear transgenes in the green alga Chlamydomonas: synthesis of an HIV antigen and development of a new selectable marker

(2016) Rouhollah Barahimipour et al.   PLANT MOLECULAR BIOLOGY

Synthetic promoters capable of driving robust nuclear gene expression in the green alga Chlamydomonas reinhardtii

(2016) Melissa A. Scranton et al.   Algal Research-Biomass Biofuels and Bioproducts

Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit

(2015) Kyle J. Lauersen et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Investigating the dynamics of recombinant protein secretion from a microalgal host

(2015) Kyle J. Lauersen et al.   JOURNAL OF BIOTECHNOLOGY

Ice recrystallization inhibition mediated by a nuclear-expressed and -secreted recombinant ice-binding protein in the microalga Chlamydomonas reinhardtii

(2013) Kyle J. Lauersen et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Efficient recombinant protein production and secretion from nuclear transgenes in Chlamydomonas reinhardtii

(2012) Kyle J. Lauersen et al.   JOURNAL OF BIOTECHNOLOGY

Engineering phosphorus metabolism in plants to produce a dual fertilization and weed control system

(2012) Damar Lizbeth López-Arredondo et al.   NATURE BIOTECHNOLOGY

A revised mineral nutrient supplement increases biomass and growth rate in Chlamydomonas reinhardtii

(2011) Janette Kropat et al.   PLANT JOURNAL

Generation ofChlamydomonasstrains that efficiently express nuclear transgenes

(2008) Juliane Neupert et al.   PLANT JOURNAL