Stable kinetochore‐microtubule attachment requires loop‐dependent Ndc80‐Ndc80 binding

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Small changes in phospho-occupancy at the kinetochore–microtubule interface drive mitotic fidelity

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Three-dimensional structure of kinetochore-fibers in human mitotic spindles

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Ensemble-Level Organization of Human Kinetochores and Evidence for Distinct Tension and Attachment Sensors

(2020) Emanuele Roscioli et al.   Cell Reports

The Hec1/Ndc80 tail domain is required for force generation at kinetochores, but is dispensable for kinetochore–microtubule attachment formation and Ska complex recruitment

(2020) Robert T. Wimbish et al.   MOLECULAR BIOLOGY OF THE CELL

Tight bending of the Ndc80 complex provides intrinsic regulation of its binding to microtubules

(2019) Emily Anne Scarborough et al.   eLife

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Electron tomography reveals aspects of spindle structure important for mechanical stability at metaphase

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Molecular determinants of the Ska-Ndc80 interaction and their influence on microtubule tracking and force-coupling

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Human Ska complex and Ndc80 complex interact to form a load-bearing assembly that strengthens kinetochore–microtubule attachments

(2018) Luke A. Helgeson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface

(2018) Simon Jenni et al.   SCIENCE

Multivalency of NDC80 in the outer kinetochore is essential to track shortening microtubules and generate forces

(2018) Vladimir A Volkov et al.   eLife

Simplified Protocol for Cross-linking Mass Spectrometry Using the MS-Cleavable Cross-linker DSBU with Efficient Cross-link Identification

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The kinetochore–microtubule interface at a glance

(2018) Julie K. Monda et al.   JOURNAL OF CELL SCIENCE

Measuring NDC80 binding reveals the molecular basis of tension-dependent kinetochore-microtubule attachments

(2018) Tae Yeon Yoo et al.   eLife

Aurora A kinase phosphorylates Hec1 to regulate metaphase kinetochore–microtubule dynamics

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UCSF ChimeraX: Meeting modern challenges in visualization and analysis

(2017) Thomas D. Goddard et al.   PROTEIN SCIENCE

Aurora-B kinase pathway controls the lateral to end-on conversion of kinetochore-microtubule attachments in human cells

(2017) Roshan L. Shrestha et al.   Nature Communications

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biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes

(2016) Florian Weissmann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Conserved Tetramer Junction in the Kinetochore Ndc80 Complex

(2016) Roberto Valverde et al.   Cell Reports

Molecular basis of outer kinetochore assembly on CENP-T

(2016) Pim J Huis in 't Veld et al.   eLife

Ca2+-independent sortase-A exhibits high selective protein ligation activity in the cytoplasm ofEscherichia coli

(2015) Hidehiko Hirakawa et al.   Biotechnology Journal

Quest for Orthologs Entails Quest for Tree of Life: In Search of the Gene Stream

(2015) Brigitte Boeckmann et al.   Genome Biology and Evolution

Multiple assembly mechanisms anchor the KMN spindle checkpoint platform at human mitotic kinetochores

(2015) Soonjoung Kim et al.   JOURNAL OF CELL BIOLOGY

Multisite phosphorylation of the NDC80 complex gradually tunes its microtubule-binding affinity

(2015) Anatoly V. Zaytsev et al.   MOLECULAR BIOLOGY OF THE CELL

Stable kinetochore–microtubule attachment is sufficient to silence the spindle assembly checkpoint in human cells

(2015) Eric C. Tauchman et al.   Nature Communications

Kinetochore–microtubule attachment is sufficient to satisfy the human spindle assembly checkpoint

(2015) Banafsheh Etemad et al.   Nature Communications

A quantitative description of Ndc80 complex linkage to human kinetochores

(2015) Aussie Suzuki et al.   Nature Communications

Ca2+-independent sortase-A exhibits high selective protein ligation activity in the cytoplasm ofEscherichia coli

(2015) Hidehiko Hirakawa et al.   Biotechnology Journal

The Aurora B Kinase in Chromosome Bi-Orientation and Spindle Checkpoint Signaling

(2015) Veronica Krenn et al.   Frontiers in Oncology

SpyAvidin Hubs Enable Precise and Ultrastable Orthogonal Nanoassembly

(2014) Michael Fairhead et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Lateral to End-on Conversion of Chromosome-Microtubule Attachment Requires Kinesins CENP-E and MCAK

(2013) Roshan L. Shrestha et al.   CURRENT BIOLOGY

Phosphorylation of Microtubule-binding Protein Hec1 by Mitotic Kinase Aurora B Specifies Spindle Checkpoint Kinase Mps1 Signaling at the Kinetochore

(2013) Tongge Zhu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Molecular requirements for the formation of a kinetochore–microtubule interface by Dam1 and Ndc80 complexes

(2013) Fabienne Lampert et al.   JOURNAL OF CELL BIOLOGY

The internal loop of fission yeast Ndc80 binds Alp7/TACC-Alp14/TOG and ensures proper chromosome attachment

(2013) Ngang Heok Tang et al.   MOLECULAR BIOLOGY OF THE CELL

The Ndc80 internal loop is required for recruitment of the Ska complex to establish end-on microtubule attachment to kinetochores

(2012) Gang Zhang et al.   JOURNAL OF CELL SCIENCE

Recruitment of the human Cdt1 replication licensing protein by the loop domain of Hec1 is required for stable kinetochore–microtubule attachment

(2012) Dileep Varma et al.   NATURE CELL BIOLOGY

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

Multimodal microtubule binding by the Ndc80 kinetochore complex

(2012) Gregory M Alushin et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

The Spatial Arrangement of Chromosomes during Prometaphase Facilitates Spindle Assembly

(2011) Valentin Magidson et al.   CELL

The Ndc80 Loop Region Facilitates Formation of Kinetochore Attachment to the Dynamic Microtubule Plus End

(2011) Jean-François Maure et al.   CURRENT BIOLOGY

Ndc80 Internal Loop Interacts with Dis1/TOG to Ensure Proper Kinetochore-Spindle Attachment in Fission Yeast

(2011) Kuo-Shun Hsu et al.   CURRENT BIOLOGY

Temporal changes in Hec1 phosphorylation control kinetochore-microtubule attachment stability during mitosis

(2011) K. F. DeLuca et al.   JOURNAL OF CELL SCIENCE

The Ndc80 complex uses a tripartite attachment point to couple microtubule depolymerization to chromosome movement

(2011) John G. Tooley et al.   MOLECULAR BIOLOGY OF THE CELL

The NDC80 complex proteins Nuf2 and Hec1 make distinct contributions to kinetochore–microtubule attachment in mitosis

(2011) Lynsie J.R. Sundin et al.   MOLECULAR BIOLOGY OF THE CELL

Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine

(2010) Stefano Santaguida et al.   JOURNAL OF CELL BIOLOGY

Tension directly stabilizes reconstituted kinetochore-microtubule attachments

(2010) Bungo Akiyoshi et al.   NATURE

The Ndc80 kinetochore complex forms oligomeric arrays along microtubules

(2010) Gregory M. Alushin et al.   NATURE

A streptavidin variant with slower biotin dissociation and increased mechanostability

(2010) Claire E Chivers et al.   NATURE METHODS

Jalview Version 2--a multiple sequence alignment editor and analysis workbench

(2009) A. M. Waterhouse et al.   BIOINFORMATICS

The Ndc80 Kinetochore Complex Forms Load-Bearing Attachments to Dynamic Microtubule Tips via Biased Diffusion

(2009) Andrew F. Powers et al.   CELL

Implications for Kinetochore-Microtubule Attachment from the Structure of an Engineered Ndc80 Complex

(2008) Claudio Ciferri et al.   CELL

Kinetochore-Microtubule Attachment Relies on the Disordered N-Terminal Tail Domain of Hec1

(2008) Geoffrey J. Guimaraes et al.   CURRENT BIOLOGY

Kinetochore Attachments Require an Interaction between Unstructured Tails on Microtubules and Ndc80Hec1

(2008) Stephanie A. Miller et al.   CURRENT BIOLOGY

Architecture and Flexibility of the Yeast Ndc80 Kinetochore Complex

(2008) Hong-Wei Wang et al.   JOURNAL OF MOLECULAR BIOLOGY