Structural specializations of the sperm tail

In situ cryo-electron tomography reveals the asymmetric architecture of mammalian sperm axonemes

(2023) Zhen Chen et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Axonemal structures reveal mechanoregulatory and disease mechanisms

(2023) Travis Walton et al.   NATURE

Structural and functional insight into regulation of kinesin-1 by microtubule-associated protein MAP7

(2022) Luke S. Ferro et al.   SCIENCE

Fasting improves therapeutic response in hepatocellular carcinoma through p53-dependent metabolic synergism

(2022) Jelena Krstic et al.   Science Advances

Ciliary central apparatus structure reveals mechanisms of microtubule patterning

(2022) Miao Gui et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Dali server: structural unification of protein families

(2022) Liisa Holm   NUCLEIC ACIDS RESEARCH

DeepTracer-ID: De novo protein identification from cryo-EM maps

(2022) Luca Chang et al.   BIOPHYSICAL JOURNAL

SPACA9 is a lumenal protein of human ciliary singlet and doublet microtubules

(2022) Miao Gui et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Analysis of copy number variation in men with non‐obstructive azoospermia

(2022) M. J. Wyrwoll et al.   Andrology

Three-dimensional flagella structures from animals’ closest unicellular relatives, the Choanoflagellates

(2022) Justine M Pinskey et al.   eLife

Tubulin glycylation controls axonemal dynein activity, flagellar beat, and male fertility

(2021) Sudarshan Gadadhar et al.   SCIENCE

The multi‐scale architecture of mammalian sperm flagella and implications for ciliary motility

(2021) Miguel Ricardo Leung et al.   EMBO JOURNAL

Cryo-EM structure of a microtubule-bound parasite kinesin motor and implications for its mechanism and inhibition

(2021) Alexander D. Cook et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Highly accurate protein structure prediction with AlphaFold

(2021) John Jumper et al.   NATURE

Cryo-EM structure of cortical microtubules from human parasite Toxoplasma gondii identifies their microtubule inner proteins

(2021) Xiangli Wang et al.   Nature Communications

Fertilization mode drives sperm length evolution across the animal tree of life

(2021) Ariel F. Kahrl et al.   Nature Ecology & Evolution

DeepEMhancer: a deep learning solution for cryo-EM volume post-processing

(2021) Ruben Sanchez-Garcia et al.   Communications Biology

De novo identification of mammalian ciliary motility proteins using cryo-EM

(2021) Miao Gui et al.   CELL

TSSK3, a novel target for male contraception, is required for spermiogenesis

(2021) Saman Nayyab et al.   MOLECULAR REPRODUCTION AND DEVELOPMENT

In-cell structures of conserved supramolecular protein arrays at the mitochondria–cytoskeleton interface in mammalian sperm

(2021) Miguel Ricardo Leung et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

findMySequence: a neural-network-based approach for identification of unknown proteins in X-ray crystallography and cryo-EM

(2021) Grzegorz Chojnowski et al.   IUCrJ

Testis-specific serine kinase protein family in male fertility and as targets for non-hormonal male contraception†

(2020) Ana M Salicioni et al.   BIOLOGY OF REPRODUCTION

Propulsive nanomachines: the convergent evolution of archaella, flagella, and cilia

(2020) Morgan Beeby et al.   FEMS MICROBIOLOGY REVIEWS

MAP6 is an intraluminal protein that induces neuronal microtubules to coil

(2020) Camille Cuveillier et al.   Science Advances

Towards a roadmap to advance non-hormonal contraceptive multipurpose prevention technologies: strategic insights from key stakeholders†

(2020) Anke Hemmerling et al.   BIOLOGY OF REPRODUCTION

Cfap97d1 is important for flagellar axoneme maintenance and male mouse fertility

(2020) Seiya Oura et al.   PLoS Genetics

DeepTracer for fast de novo cryo-EM protein structure modeling and special studies on CoV-related complexes

(2020) Jonas Pfab et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Loss-of-function mutations in QRICH2 cause male infertility with multiple morphological abnormalities of the sperm flagella

(2019) Ying Shen et al.   Nature Communications

Inner lumen proteins stabilize doublet microtubules in cilia and flagella

(2019) Mikito Owa et al.   Nature Communications

Flagellar ultrastructure suppresses buckling instabilities and enables mammalian sperm navigation in high-viscosity media

(2019) Hermes Gadêlha et al.   Journal of the Royal Society Interface

Taxon-specific expansion and loss of tektins inform metazoan ciliary diversity

(2019) Benjamin R. Bastin et al.   BMC EVOLUTIONARY BIOLOGY

PACRG and FAP20 form the inner junction of axonemal doublet microtubules and regulate ciliary motility

(2019) Erin E. Dymek et al.   MOLECULAR BIOLOGY OF THE CELL

Comprehensive analysis of mouse CTA functions in cancer cells and roles of TEKT5 in cancer cells and testicular germ cells

(2019) Nana Aoki et al.   MOLECULAR AND CELLULAR BIOLOGY

The present crisis in male reproductive health: an urgent need for a political, social, and research roadmap

(2019) Christopher De Jonge et al.   Andrology

The forgotten men: rising rates of male infertility urgently require new approaches for its prevention, diagnosis and treatment

(2019) Vardit Ravitsky et al.   BIOLOGY OF REPRODUCTION

Structure of the Decorated Ciliary Doublet Microtubule

(2019) Meisheng Ma et al.   CELL

A microtubule RELION-based pipeline for cryo-EM image processing

(2019) Alexander D. Cook et al.   JOURNAL OF STRUCTURAL BIOLOGY

Real-time cryo-electron microscopy data preprocessing with Warp

(2019) Dimitry Tegunov et al.   NATURE METHODS

Cryo electron tomography with Volta phase plate reveals novel structural foundations of the 96-nm axonemal repeat in the pathogen Trypanosoma brucei

(2019) Simon Imhof et al.   eLife

Asymmetries in the cilia of Chlamydomonas

(2019) Susan K. Dutcher   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Near-atomic model of microtubule-tau interactions

(2018) Elizabeth H. Kellogg et al.   SCIENCE

Systematic studies of all PIH proteins in zebrafish reveal their distinct roles in axonemal dynein assembly

(2018) Hiroshi Yamaguchi et al.   eLife

Real-space refinement in PHENIX for cryo-EM and crystallography

(2018) Pavel V. Afonine et al.   Acta Crystallographica Section D-Structural Biology

Coordination of eukaryotic cilia and flagella

(2018) Kirsty Y. Wan   Essays in Biochemistry

Efficient and robust proteome-wide approaches for cross-linking mass spectrometry

(2018) Oleg Klykov et al.   Nature Protocols

New tools for automated high-resolution cryo-EM structure determination in RELION-3

(2018) Jasenko Zivanov et al.   eLife

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

UCSF ChimeraX: Meeting modern challenges in visualization and analysis

(2017) Thomas D. Goddard et al.   PROTEIN SCIENCE

Functional anatomy of the mammalian sperm flagellum

(2016) Charles B. Lindemann et al.   Cytoskeleton

Gctf: Real-time CTF determination and correction

(2016) Kai Zhang   JOURNAL OF STRUCTURAL BIOLOGY

Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice

(2016) Haruhiko Miyata et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Testis-specific serine/threonine protein kinase 4 (Tssk4) phosphorylates Odf2 at Ser-76

(2016) Xiaoli Wang et al.   Scientific Reports

Association of polymorphisms in tektin-t gene with idiopathic asthenozoospermia in Sichuan, China

(2015) Shao-hong Zhang et al.   JOURNAL OF ASSISTED REPRODUCTION AND GENETICS

Human FAM154A (SAXO1) is a microtubule-stabilizing protein specific to cilia and related structures

(2015) D. Dacheux et al.   JOURNAL OF CELL SCIENCE

CTFFIND4: Fast and accurate defocus estimation from electron micrographs

(2015) Alexis Rohou et al.   JOURNAL OF STRUCTURAL BIOLOGY

A new protocol to accurately determine microtubule lattice seam location

(2015) Rui Zhang et al.   JOURNAL OF STRUCTURAL BIOLOGY

Tissue-based map of the human proteome

(2015) M. Uhlen et al.   SCIENCE

Ultrastructural evidence that motility changes caused by variations in ATP, Mg2+, and ADP correlate to conformational changes in reactivated bull sperm axonemes

(2014) Kathleen A. Lesich et al.   Cytoskeleton

Insights into the Structure and Function of Ciliary and Flagellar Doublet Microtubules

(2014) Richard Linck et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

FAP20 is an inner junction protein of doublet microtubules essential for both the planar asymmetrical waveform and stability of flagella in Chlamydomonas

(2014) Haru-aki Yanagisawa et al.   MOLECULAR BIOLOGY OF THE CELL

Tssk4 is essential for maintaining the structural integrity of sperm flagellum

(2014) Xiaoli Wang et al.   MOLECULAR HUMAN REPRODUCTION

Cryo-electron tomography reveals ciliary defects underlying human RSPH1 primary ciliary dyskinesia

(2014) Jianfeng Lin et al.   Nature Communications

Structural Basis for the Association of MAP6 Protein with Microtubules and Its Regulation by Calmodulin

(2013) Julien Lefèvre et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Human disease locus discovery and mapping to molecular pathways through phylogenetic profiling

(2013) Y. Tabach et al.   Molecular Systems Biology

Comparative structural analysis of eukaryotic flagella and cilia from Chlamydomonas, Tetrahymena, and sea urchins

(2012) Gaia Pigino et al.   JOURNAL OF STRUCTURAL BIOLOGY

The structural heterogeneity of radial spokes in cilia and flagella is conserved

(2011) Jianfeng Lin et al.   Cytoskeleton

Cryo-electron tomography reveals conserved features of doublet microtubules in flagella

(2011) D. Nicastro et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Multidimensional Analysis of Uncharacterized Sperm Proteins inCiona intestinalis: EST-Based Analysis and Functional Immunoscreening of Testis-Expressed Genes

(2011) Alu Konno et al.   ZOOLOGICAL SCIENCE

Accelerated Profile HMM Searches

(2011) Sean R. Eddy   PLoS Computational Biology

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Pitchfork Regulates Primary Cilia Disassembly and Left-Right Asymmetry

(2010) Doris Kinzel et al.   DEVELOPMENTAL CELL

Dali server: conservation mapping in 3D

(2010) Liisa Holm et al.   NUCLEIC ACIDS RESEARCH

c.822+126T>G/C: a novel triallelic polymorphism of the TSSK6 gene associated with spermatogenic impairment in a Chinese population

(2009) Dan Su et al.   ASIAN JOURNAL OF ANDROLOGY

Tssk6 is required for Izumo relocalization and gamete fusion in the mouse

(2009) J. Sosnik et al.   JOURNAL OF CELL SCIENCE

Targeted deletion of Tssk1 and 2 causes male infertility due to haploinsufficiency

(2008) Bingfang Xu et al.   DEVELOPMENTAL BIOLOGY

A possible association of a human tektin-t gene mutation (A229V) with isolated non-syndromic asthenozoospermia: Case Report

(2008) D. Zuccarello et al.   HUMAN REPRODUCTION

Mutation Screening and Association Study of the TSSK4 Gene in Chinese Infertile Men With Impaired Spermatogenesis

(2008) D. Su et al.   JOURNAL OF ANDROLOGY

Tektin 3 is required for progressive sperm motility in mice

(2008) Angshumoy Roy et al.   MOLECULAR REPRODUCTION AND DEVELOPMENT

MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification

(2008) Jürgen Cox et al.   NATURE BIOTECHNOLOGY

Microtubule Dynamics Depart from the Wormlike Chain Model

(2008) Katja M. Taute et al.   PHYSICAL REVIEW LETTERS

The tektin family of microtubule-stabilizing proteins

(2008) Linda A Amos   GENOME BIOLOGY