The multi‐scale architecture of mammalian sperm flagella and implications for ciliary motility

The inner junction complex of the cilia is an interaction hub that involves tubulin post-translational modifications

(2020) Ahmad Abdelzaher Zaki Khalifa et al.   eLife

A helical inner scaffold provides a structural basis for centriole cohesion

(2020) Maeva Le Guennec et al.   Science Advances

Human sperm uses asymmetric and anisotropic flagellar controls to regulate swimming symmetry and cell steering

(2020) Hermes Gadêlha et al.   Science Advances

Inner lumen proteins stabilize doublet microtubules in cilia and flagella

(2019) Mikito Owa et al.   Nature Communications

Flagellar ultrastructure suppresses buckling instabilities and enables mammalian sperm navigation in high-viscosity media

(2019) Hermes Gadêlha et al.   Journal of the Royal Society Interface

Axonemal doublet microtubules can split into two complete singlets in human sperm flagellum tips

(2019) Davide Zabeo et al.   FEBS LETTERS

Mind the gap: Micro-expansion joints drastically decrease the bending of FIB-milled cryo-lamellae

(2019) Georg Wolff et al.   JOURNAL OF STRUCTURAL BIOLOGY

Tubulin lattice in cilia is in a stressed form regulated by microtubule inner proteins

(2019) Muneyoshi Ichikawa et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of the Decorated Ciliary Doublet Microtubule

(2019) Meisheng Ma et al.   CELL

Structural organization of the C1a-e-c supercomplex within the ciliary central apparatus

(2019) Gang Fu et al.   JOURNAL OF CELL BIOLOGY

Real-time cryo-electron microscopy data preprocessing with Warp

(2019) Dimitry Tegunov et al.   NATURE METHODS

Odf2 haploinsufficiency causes a new type of decapitated and decaudated spermatozoa, Odf2-DDS, in mice

(2019) Chizuru Ito et al.   Scientific Reports

Cryo electron tomography with Volta phase plate reveals novel structural foundations of the 96-nm axonemal repeat in the pathogen Trypanosoma brucei

(2019) Simon Imhof et al.   eLife

Electron cryotomography of intact motile cilia defines the basal body to axoneme transition

(2019) Garrett A. Greenan et al.   JOURNAL OF CELL BIOLOGY

Assembly, Functions and Evolution of Archaella, Flagella and Cilia

(2018) Shahid Khan et al.   CURRENT BIOLOGY

A novel atypical sperm centriole is functional during human fertilization

(2018) Emily L. Fishman et al.   Nature Communications

A lumenal interrupted helix in human sperm tail microtubules

(2018) Davide Zabeo et al.   Scientific Reports

Systematic studies of all PIH proteins in zebrafish reveal their distinct roles in axonemal dynein assembly

(2018) Hiroshi Yamaguchi et al.   eLife

Rapid Evolution of Sperm Produces Diverse Centriole Structures that Reveal the Most Rudimentary Structure Needed for Function

(2018) Tomer Avidor-Reiss   Cells

Sperm form and function: what do we know about the role of sexual selection?

(2018) Stefan Lüpold et al.   REPRODUCTION

Insights into centriole geometry revealed by cryotomography of doublet and triplet centrioles

(2018) Garrett A Greenan et al.   eLife

Coordination of eukaryotic cilia and flagella

(2018) Kirsty Y. Wan   Essays in Biochemistry

Outer dense fibers stabilize the axoneme to maintain sperm motility

(2017) Wenlong Zhao et al.   JOURNAL OF CELLULAR AND MOLECULAR MEDICINE

Optimized cryo-focused ion beam sample preparation aimed at in situ structural studies of membrane proteins

(2017) Miroslava Schaffer et al.   JOURNAL OF STRUCTURAL BIOLOGY

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

Convolutional neural networks for automated annotation of cellular cryo-electron tomograms

(2017) Muyuan Chen et al.   NATURE METHODS

UCSF ChimeraX: Meeting modern challenges in visualization and analysis

(2017) Thomas D. Goddard et al.   PROTEIN SCIENCE

Analysis of the small non-protein-coding RNA profile of mouse spermatozoa reveals specific enrichment of piRNAs within mature spermatozoa

(2017) Kate Hutcheon et al.   RNA Biology

Human sperm steer with second harmonics of the flagellar beat

(2017) Guglielmo Saggiorato et al.   Nature Communications

Subnanometre-resolution structure of the doublet microtubule reveals new classes of microtubule-associated proteins

(2017) Muneyoshi Ichikawa et al.   Nature Communications

Functional anatomy of the mammalian sperm flagellum

(2016) Charles B. Lindemann et al.   Cytoskeleton

Axoneme Structure from Motile Cilia

(2016) Takashi Ishikawa   Cold Spring Harbor Perspectives in Biology

Evolution of Cilia

(2016) David R. Mitchell   Cold Spring Harbor Perspectives in Biology

Intraflagellar transport is essential for mammalian spermiogenesis but is absent in mature sperm

(2015) Jovenal T. San Agustin et al.   MOLECULAR BIOLOGY OF THE CELL

High-yield and high-purity isolation of hepatic stellate cells from normal and fibrotic mouse livers

(2015) Ingmar Mederacke et al.   Nature Protocols

Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive

(2015) H. Miyata et al.   SCIENCE

Episodic rolling and transient attachments create diversity in sperm swimming behavior

(2014) Donner F Babcock et al.   BMC BIOLOGY

Ultrastructural evidence that motility changes caused by variations in ATP, Mg2+, and ADP correlate to conformational changes in reactivated bull sperm axonemes

(2014) Kathleen A. Lesich et al.   Cytoskeleton

Structural mechanism of the dynein power stroke

(2014) Jianfeng Lin et al.   NATURE CELL BIOLOGY

Volta potential phase plate for in-focus phase contrast transmission electron microscopy

(2014) R. Danev et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cryo-electron tomography reveals ciliary defects underlying human RSPH1 primary ciliary dyskinesia

(2014) Jianfeng Lin et al.   Nature Communications

Native Architecture of the Centriole Proximal Region Reveals Features Underlying Its 9-Fold Radial Symmetry

(2013) Paul Guichard et al.   CURRENT BIOLOGY

Three-Dimensional Structure of the Bovine Sperm Connecting Piece Revealed by Electron Cryotomography1

(2012) Puey Ounjai et al.   BIOLOGY OF REPRODUCTION

Conserved structural motifs in the central pair complex of eukaryotic flagella

(2012) Blanca I. Carbajal-González et al.   Cytoskeleton

Comparative structural analysis of eukaryotic flagella and cilia from Chlamydomonas, Tetrahymena, and sea urchins

(2012) Gaia Pigino et al.   JOURNAL OF STRUCTURAL BIOLOGY

A filtered backprojection algorithm with characteristics of the iterative landweber algorithm

(2012) Gengsheng L. Zeng   MEDICAL PHYSICS

Complex sperm evolution

(2012) M. J. G. Gage   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Focused ion beam micromachining of eukaryotic cells for cryoelectron tomography

(2012) A. Rigort et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mammalian Sperm Motility: Observation and Theory

(2011) E.A. Gaffney et al.   Annual Review of Fluid Mechanics

Three-dimensional structure of basal body triplet revealed by electron cryo-tomography

(2011) Sam Li et al.   EMBO JOURNAL

Clustering and variance maps for cryo-electron tomography using wedge-masked differences

(2011) John M. Heumann et al.   JOURNAL OF STRUCTURAL BIOLOGY

Cryo-electron tomography reveals conserved features of doublet microtubules in flagella

(2011) D. Nicastro et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Speriolin is a novel human and mouse sperm centrosome protein

(2010) M. Goto et al.   HUMAN REPRODUCTION

High-precision tracking of sperm swimming fine structure provides strong test of resistive force theory

(2010) B. M. Friedrich et al.   JOURNAL OF EXPERIMENTAL BIOLOGY

Thinking about flagellar oscillation

(2009) Charles J. Brokaw   CELL MOTILITY AND THE CYTOSKELETON

Bend propagation in the flagella of migrating human sperm, and its modulation by viscosity

(2009) D. J. Smith et al.   CELL MOTILITY AND THE CYTOSKELETON

Mutations inHydinimpair ciliary motility in mice

(2008) Karl-Ferdinand Lechtreck et al.   JOURNAL OF CELL BIOLOGY

An Improved Cryogen for Plunge Freezing

(2008) William F. Tivol et al.   MICROSCOPY AND MICROANALYSIS

Microtubule Dynamics Depart from the Wormlike Chain Model

(2008) Katja M. Taute et al.   PHYSICAL REVIEW LETTERS