Therapeutic strategies of three-dimensional stem cell spheroids and organoids for tissue repair and regeneration

Recent Advances in Three-Dimensional Multicellular Spheroid Culture and Future Development

(2021) Honglin Shen et al.   Micromachines

Trabecular bone organoid model for studying the regulation of localized bone remodeling

(2021) Yongkuk Park et al.   Science Advances

Injection of hybrid 3D spheroids composed of podocytes, mesenchymal stem cells, and vascular endothelial cells into the renal cortex improves kidney function and replenishes glomerular podocytes

(2021) Wen‐Yu Yang et al.   Bioengineering & Translational Medicine

An Organoid for Woven Bone

(2021) Anat Akiva et al.   ADVANCED FUNCTIONAL MATERIALS

Increased Mesenchymal Stem Cell Functionalization in Three-Dimensional Manufacturing Settings for Enhanced Therapeutic Applications

(2021) Dimitrios Kouroupis et al.   Frontiers in Bioengineering and Biotechnology

Engineering Vascularized Organoid-on-a-Chip Models

(2021) Venktesh S. Shirure et al.   Annual Review of Biomedical Engineering

Role of Matrix-Associated Autologous Chondrocyte Implantation with Spheroids in the Treatment of Large Chondral Defects in the Knee: A Systematic Review

(2021) Lucienne Angela Vonk et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Intramyocardial delivery of human cardiac stem cell spheroids with enhanced cell engraftment ability and cardiomyogenic potential for myocardial infarct repair

(2021) Min Ah Han et al.   JOURNAL OF CONTROLLED RELEASE

Microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids

(2021) Ann-Na Cho et al.   Nature Communications

Plasma-assisted multiscale topographic scaffolds for soft and hard tissue regeneration

(2021) Woochan Kim et al.   npj Regenerative Medicine

Ca2+-supplying black phosphorus-based scaffolds fabricated with microfluidic technology for osteogenesis

(2021) Zhanrong Li et al.   Bioactive Materials

All-in-One Hydrogel Realizing Adipose-Derived Stem Cell Spheroid Production and In Vivo Injection via “Gel–Sol” Transition for Angiogenesis in Hind Limb Ischemia

(2020) Yuhao Hong et al.   ACS Applied Materials & Interfaces

Safety and efficacy of matrix-associated autologous chondrocyte implantation with spheroid technology is independent of spheroid dose after 4 years

(2020) Philipp Niemeyer et al.   KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY

Cardioprotective effects of genetically engineered cardiac stem cells by spheroid formation on ischemic cardiomyocytes

(2020) Han Saem Jeong et al.   MOLECULAR MEDICINE

Integrin-specific hydrogels modulate transplanted human bone marrow-derived mesenchymal stem cell survival, engraftment, and reparative activities

(2020) Amy Y. Clark et al.   Nature Communications

Matrix-Associated Autologous Chondrocyte Implantation with Spheroid Technology Is Superior to Arthroscopic Microfracture at 36 Months Regarding Activities of Daily Living and Sporting Activities after Treatment

(2020) Arnd Hoburg et al.   Cartilage

Human adipose–derived mesenchymal stem cell–based medical microrobot system for knee cartilage regeneration in vivo

(2020) Gwangjun Go et al.   Science Robotics

High‐Throughput Differentiation of Embryonic Stem Cells into Cardiomyocytes with a Microfabricated Magnetic Pattern and Cyclic Stimulation

(2020) Gaëtan Mary et al.   ADVANCED FUNCTIONAL MATERIALS

Polymer Hydrogels to Guide Organotypic and Organoid Cultures

(2020) Valentina Magno et al.   ADVANCED FUNCTIONAL MATERIALS

Structure and ingredient-based biomimetic scaffolds combining with autologous bone marrow-derived mesenchymal stem cell sheets for bone-tendon healing

(2020) Yifu Tang et al.   BIOMATERIALS

Excellent histological results in terms of articular cartilage regeneration after spheroid-based autologous chondrocyte implantation (ACI)

(2020) David Grevenstein et al.   KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY

Scaffold‐Free Bio‐3D Printing Using Spheroids as “Bio‐Inks” for Tissue (Re‐)Construction and Drug Response Tests

(2020) Daiki Murata et al.   Advanced Healthcare Materials

Development of a Novel Hanging Drop Platform for Engineering Controllable 3D Microenvironments

(2020) Chin-Yi Cho et al.   Frontiers in Cell and Developmental Biology

Tendon-Inspired Nanotopographic Scaffold for Tissue Regeneration in Rotator Cuff Injuries

(2020) Woochan Kim et al.   ACS Omega

The Effect of Thiol Structure on Allyl Sulfide Photodegradable Hydrogels and their Application as a Degradable Scaffold for Organoid Passaging

(2020) F. Max Yavitt et al.   ADVANCED MATERIALS

Stem cell-laden injectable hydrogel microspheres for cancellous bone regeneration

(2020) Jiezhou Wu et al.   CHEMICAL ENGINEERING JOURNAL

Hair-bearing human skin generated entirely from pluripotent stem cells

(2020) Jiyoon Lee et al.   NATURE

High-throughput automated organoid culture via stem-cell aggregation in microcavity arrays

(2020) Nathalie Brandenberg et al.   Nature Biomedical Engineering

Thermosensitive Injectable Chitosan/Collagen/β-Glycerophosphate Composite Hydrogels for Enhancing Wound Healing by Encapsulating Mesenchymal Stem Cell Spheroids

(2020) Ming Yang et al.   ACS Omega

Injectable Therapeutic Organoids Using Sacrificial Hydrogels

(2020) Ninna S. Rossen et al.   iScience

Alginate/laponite hydrogel microspheres co-encapsulating dental pulp stem cells and VEGF for endodontic regeneration

(2020) Ruitao Zhang et al.   Acta Biomaterialia

Human adipose-derived stem cell spheroids incorporating platelet-derived growth factor (PDGF) and bio-minerals for vascularized bone tissue engineering

(2020) Jinkyu Lee et al.   BIOMATERIALS

iPSC-Derived Liver Organoids: A Journey from Drug Screening, to Disease Modeling, Arriving to Regenerative Medicine

(2020) Cristina Olgasi et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Scaffold-based and Scaffold-free Strategies in Dental Pulp Regeneration

(2020) Waruna Lakmal Dissanayaka et al.   JOURNAL OF ENDODONTICS

Homeostatic mini-intestines through scaffold-guided organoid morphogenesis

(2020) Mikhail Nikolaev et al.   NATURE

Mesenchymal Stem Cell Spheroids Embedded in an Injectable Thermosensitive Hydrogel: An In Situ Drug Formation Platform for Accelerated Wound Healing

(2020) Mohammad Ali Nilforoushzadeh et al.   ACS Biomaterials-Science & Engineering

Microtechnology-based methods for organoid models

(2020) Vanessa Velasco et al.   Microsystems & Nanoengineering

Convergence of 3D printed biomimetic wound dressings and adult stem cell therapy

(2020) Abbas Shafiee et al.   BIOMATERIALS

A review of manufacturing capabilities of cell spheroid generation technologies and future development

(2020) Dan Liu et al.   BIOTECHNOLOGY AND BIOENGINEERING

Human embryonic stem cell-derived organoid retinoblastoma reveals a cancerous origin

(2020) Hui Liu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Enzyme-Crosslinked Gelatin Hydrogel with Adipose-Derived Stem Cell Spheroid Facilitating Wound Repair in the Murine Burn Model

(2020) Ting-Yu Lu et al.   Polymers

A matrigel-free method to generate matured human cerebral organoids using 3D-Printed microwell arrays

(2020) Cheng Chen et al.   Bioactive Materials

Human blood vessel organoids as a model of diabetic vasculopathy

(2019) Reiner A. Wimmer et al.   NATURE

Nonadhesive Alginate Hydrogels Support Growth of Pluripotent Stem Cell-Derived Intestinal Organoids

(2019) Meghan M. Capeling et al.   Stem Cell Reports

Synergistic interplay between human MSCs and HUVECs in 3D spheroids laden in collagen/fibrin hydrogels for bone tissue engineering

(2019) Dong Nyoung Heo et al.   Acta Biomaterialia

Mechanical Property of Hydrogels and the Presence of Adipose Stem Cells in Tumor Stroma Affect Spheroid Formation in the 3D Osteosarcoma Model

(2019) B. Kundu et al.   ACS Applied Materials & Interfaces

Advances in Hydrogels in Organoids and Organs‐on‐a‐Chip

(2019) Haitao Liu et al.   ADVANCED MATERIALS

Oriented collagen fiber membranes formed through counter-rotating extrusion and their application in tendon regeneration

(2019) Shuang Yang et al.   BIOMATERIALS

Engineering Stem Cell Self-organization to Build Better Organoids

(2019) Jonathan A. Brassard et al.   Cell Stem Cell

An Air Bubble-Isolating Rotating Wall Vessel Bioreactor for Improved Spheroid/Organoid Formation

(2019) Michael Alexander Phelan et al.   TISSUE ENGINEERING PART C-METHODS

Dual stem cell therapy synergistically improves cardiac function and vascular regeneration following myocardial infarction

(2019) Soon-Jung Park et al.   Nature Communications

Hydrogel Nanospike Patch as a Flexible Anti-Pathogenic Scaffold for Regulating Stem Cell Behavior

(2019) Sunho Park et al.   ACS Nano

Therapeutic potential of spheroids of stem cells from human exfoliated deciduous teeth for chronic liver fibrosis and hemophilia A

(2019) Yoshiaki Takahashi et al.   PEDIATRIC SURGERY INTERNATIONAL

Stem cell-based bone and dental regeneration: a view of microenvironmental modulation

(2019) Chenxi Zheng et al.   International Journal of Oral Science

Merging organoid and organ-on-a-chip technology to generate complex multi-layer tissue models in a human retina-on-a-chip platform

(2019) Kevin Achberger et al.   eLife

Engineered materials for organoid systems

(2019) Michael J. Kratochvil et al.   Nature Reviews Materials

Biocompatible and Biodegradable 3D Double-Network Fibrous Scaffold for Excellent Cell Growth

(2019) Zhanrong Li et al.   Journal of Biomedical Nanotechnology

Automated real-time monitoring of human pluripotent stem cell aggregation in stirred tank reactors

(2019) Ivo Schwedhelm et al.   Scientific Reports

Stem cell spheroids incorporating fibers coated with adenosine and polydopamine as a modular building blocks for bone tissue engineering

(2019) Taufiq Ahmad et al.   BIOMATERIALS

Lotus seedpod-inspired hydrogels as an all-in-one platform for culture and delivery of stem cell spheroids

(2019) Se-jeong Kim et al.   BIOMATERIALS

The Regenerative Effect of Portal Vein Injection of Liver Organoids by Retrorsine/Partial Hepatectomy in Rats

(2019) Tomonori Tsuchida et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Magnetoresponsive stem cell spheroid-based cartilage recovery platform utilizing electromagnetic fields

(2019) Ami Yoo et al.   SENSORS AND ACTUATORS B-CHEMICAL

Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture

(2019) Giovanni Giuseppe Giobbe et al.   Nature Communications

Cerebral Organoids Repair Ischemic Stroke Brain Injury

(2019) Shu-Na Wang et al.   Translational Stroke Research

Developmentally Engineered Callus Organoid Bioassemblies Exhibit Predictive In Vivo Long Bone Healing

(2019) Gabriella Nilsson Hall et al.   Advanced Science

Spheroid Culture System Methods and Applications for Mesenchymal Stem Cells

(2019) Na-Eun Ryu et al.   Cells

Directing Induced Pluripotent Stem Cell Derived Neural Stem Cell Fate with a Three-Dimensional Biomimetic Hydrogel for Spinal Cord Injury Repair

(2018) Lei Fan et al.   ACS Applied Materials & Interfaces

Component effect of stem cell-loaded thermosensitive polypeptide hydrogels on cartilage repair

(2018) He Liu et al.   Acta Biomaterialia

Injectable degradable PVA microgels prepared by microfluidic technology for controlled osteogenic differentiation of mesenchymal stem cells

(2018) Yong Hou et al.   Acta Biomaterialia

Functional tissue-engineered microtissue derived from cartilage extracellular matrix for articular cartilage regeneration

(2018) Heyong Yin et al.   Acta Biomaterialia

A hybrid injectable hydrogel from hyperbranched PEG macromer as a stem cell delivery and retention platform for diabetic wound healing

(2018) Qian Xu et al.   Acta Biomaterialia

Fabrication of in vitro 3D mineralized tissue by fusion of composite spheroids incorporating biomineral-coated nanofibers and human adipose-derived stem cells

(2018) Taufiq Ahmad et al.   Acta Biomaterialia

Cell sheet composed of adipose-derived stem cells demonstrates enhanced skin wound healing with reduced scar formation

(2018) Jiashing Yu et al.   Acta Biomaterialia

3D heterogeneous islet organoid generation from human embryonic stem cells using a novel engineered hydrogel platform

(2018) Joseph Candiello et al.   BIOMATERIALS

Engineering spheroids potentiating cell-cell and cell-ECM interactions by self-assembly of stem cell microlayer

(2018) Yu Bin Lee et al.   BIOMATERIALS

3D neural tissue models: From spheroids to bioprinting

(2018) Pei Zhuang et al.   BIOMATERIALS

Engineering innervated secretory epithelial organoids by magnetic three-dimensional bioprinting for stimulating epithelial growth in salivary glands

(2018) Christabella Adine et al.   BIOMATERIALS

Optimal electrical stimulation boosts stem cell therapy in nerve regeneration

(2018) Jian Du et al.   BIOMATERIALS

In vivo therapeutic applications of cell spheroids

(2018) Chin Siang Ong et al.   BIOTECHNOLOGY ADVANCES

Pulp Regeneration by 3-dimensional Dental Pulp Stem Cell Constructs

(2018) Y. Itoh et al.   JOURNAL OF DENTAL RESEARCH

A three-dimensional neural spheroid model for capillary-like network formation

(2018) Molly E. Boutin et al.   JOURNAL OF NEUROSCIENCE METHODS

Human brain organoid-on-a-chip to model prenatal nicotine exposure

(2018) Yaqing Wang et al.   LAB ON A CHIP

Generation of human brain region–specific organoids using a miniaturized spinning bioreactor

(2018) Xuyu Qian et al.   Nature Protocols

Generation of spatial-patterned early-developing cardiac organoids using human pluripotent stem cells

(2018) Plansky Hoang et al.   Nature Protocols

Spheroids-on-a-chip: Recent advances and design considerations in microfluidic platforms for spheroid formation and culture

(2018) Khashayar Moshksayan et al.   SENSORS AND ACTUATORS B-CHEMICAL

Hypoxic Preconditioning of Mesenchymal Stem Cells with Subsequent Spheroid Formation Accelerates Repair of Segmental Bone Defects

(2018) Steve S. Ho et al.   STEM CELLS

Neural Differentiation of Spheroids Derived from Human Induced Pluripotent Stem Cells–Mesenchymal Stem Cells Coculture

(2018) Liqing Song et al.   TISSUE ENGINEERING PART A

Scaffold-free three-dimensional cell culturing using magnetic levitation

(2018) Esra Türker et al.   Biomaterials Science

Accelerated and Improved Differentiation of Retinal Organoids from Pluripotent Stem Cells in Rotating-Wall Vessel Bioreactors

(2018) Tyler DiStefano et al.   Stem Cell Reports

A Simple Bioreactor-Based Method to Generate Kidney Organoids from Pluripotent Stem Cells

(2018) Aneta Przepiorski et al.   Stem Cell Reports

Hydrogel microenvironments for cancer spheroid growth and drug screening

(2018) Yunfeng Li et al.   Science Advances

Functionalized Scaffold for in Situ Efficient Gene Transfection of Mesenchymal Stem Cells Spheroids toward Chondrogenesis

(2018) Kunxi Zhang et al.   ACS Applied Materials & Interfaces

Cartilage regeneration using arthroscopic flushing fluid-derived mesenchymal stem cells encapsulated in a one-step rapid cross-linked hydrogel

(2018) Jun Li et al.   Acta Biomaterialia

Preconditioning of bone marrow-derived mesenchymal stem cells with N-acetyl-L-cysteine enhances bone regeneration via reinforced resistance to oxidative stress

(2018) Jun Watanabe et al.   BIOMATERIALS

Discovery of a periosteal stem cell mediating intramembranous bone formation

(2018) Shawon Debnath et al.   NATURE

Deciduous autologous tooth stem cells regenerate dental pulp after implantation into injured teeth

(2018) Kun Xuan et al.   Science Translational Medicine

Efficient scalable production of therapeutic microvesicles derived from human mesenchymal stem cells

(2018) Jae Min Cha et al.   Scientific Reports

Hydrogels with an embossed surface: An all-in-one platform for mass production and culture of human adipose-derived stem cell spheroids

(2018) Se-jeong Kim et al.   BIOMATERIALS

Engineered tendon-fibrocartilage-bone composite and bone marrow-derived mesenchymal stem cell sheet augmentation promotes rotator cuff healing in a non-weight-bearing canine model

(2018) Qian Liu et al.   BIOMATERIALS

Human cardiomyocytes undergo enhanced maturation in embryonic stem cell-derived organoid transplants

(2018) Fahimeh Varzideh et al.   BIOMATERIALS

Adipose-derived stem cell therapies for bone regeneration

(2017) Marta Barba et al.   EXPERT OPINION ON BIOLOGICAL THERAPY

Dental Pulp Tissue Regeneration Using Dental Pulp Stem Cells Isolated and Expanded in Human Serum

(2017) Evandro Piva et al.   JOURNAL OF ENDODONTICS

Advances in multicellular spheroids formation

(2017) X. Cui et al.   Journal of the Royal Society Interface

In vivo genome editing and organoid transplantation models of colorectal cancer and metastasis

(2017) Jatin Roper et al.   NATURE BIOTECHNOLOGY

Synthetic hydrogels for human intestinal organoid generation and colonic wound repair

(2017) Ricardo Cruz-Acuña et al.   NATURE CELL BIOLOGY

Modeling Physiological Events in 2D vs. 3D Cell Culture

(2017) Kayla Duval et al.   PHYSIOLOGY

Use of CRISPR-modified human stem cell organoids to study the origin of mutational signatures in cancer

(2017) Jarno Drost et al.   SCIENCE

Life is 3D: Boosting Spheroid Function for Tissue Engineering

(2017) Matthias W. Laschke et al.   TRENDS IN BIOTECHNOLOGY

Multiscale cytometry and regulation of 3D cell cultures on a chip

(2017) Sébastien Sart et al.   Nature Communications

Bioengineered constructs combined with exercise enhance stem cell-mediated treatment of volumetric muscle loss

(2017) Marco Quarta et al.   Nature Communications

Pre-vascularization Enhances Therapeutic Effects of Human Mesenchymal Stem Cell Sheets in Full Thickness Skin Wound Repair

(2017) Lei Chen et al.   Theranostics

Massive and Reproducible Production of Liver Buds Entirely from Human Pluripotent Stem Cells

(2017) Takanori Takebe et al.   Cell Reports

Microfluidic Technology for the Generation of Cell Spheroids and Their Applications

(2017) Raja Vadivelu et al.   Micromachines

Stem cell-based bone regeneration in diseased microenvironments: Challenges and solutions

(2017) Bing-Dong Sui et al.   BIOMATERIALS

A Quiescent, Regeneration-Responsive Tissue Engineered Mesenchymal Stem Cell Bone Marrow Niche Model via Magnetic Levitation

(2016) Emily Elizabeth Louise Lewis et al.   ACS Nano

Mesenchymal stem cells in tendon repair and regeneration: basic understanding and translational challenges

(2016) Daniel J. Leong et al.   Annals of the New York Academy of Sciences

Brain-Region-Specific Organoids Using Mini-bioreactors for Modeling ZIKV Exposure

(2016) Xuyu Qian et al.   CELL

Engineering Stem Cell Organoids

(2016) Xiaolei Yin et al.   Cell Stem Cell

Organoids: Modeling Development and the Stem Cell Niche in a Dish

(2016) Kai Kretzschmar et al.   DEVELOPMENTAL CELL

Organoid-on-a-chip and body-on-a-chip systems for drug screening and disease modeling

(2016) Aleksander Skardal et al.   DRUG DISCOVERY TODAY

Adaptable stirred-tank culture strategies for large scale production of multicellular spheroid-based tumor cell models

(2016) Vítor E. Santo et al.   JOURNAL OF BIOTECHNOLOGY

3D spheroid culture enhances survival and therapeutic capacities of MSCs injected into ischemic kidney

(2016) Yong Xu et al.   JOURNAL OF CELLULAR AND MOLECULAR MEDICINE

Nanowires and Electrical Stimulation Synergistically Improve Functions of hiPSC Cardiac Spheroids

(2016) Dylan J. Richards et al.   NANO LETTERS

Designer matrices for intestinal stem cell and organoid culture

(2016) Nikolce Gjorevski et al.   NATURE

A 3D bioprinting system to produce human-scale tissue constructs with structural integrity

(2016) Hyun-Wook Kang et al.   NATURE BIOTECHNOLOGY

Quantitative proteomic analyses of mammary organoids reveals distinct signatures after exposure to environmental chemicals

(2016) Katherine E. Williams et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Viscoelastic lithography for fabricating self-organizing soft micro-honeycomb structures with ultra-high aspect ratios

(2016) Gi Seok Jeong et al.   Nature Communications

Alginate–marine collagen–agarose composite hydrogels as matrices for biomimetic 3D cell spheroid formation

(2016) Suil Shin et al.   RSC Advances

Regeneration of hyaline-like cartilage and subchondral bone simultaneously by poly(l-glutamic acid) based osteochondral scaffolds with induced autologous adipose derived stem cells

(2016) Kunxi Zhang et al.   Journal of Materials Chemistry B

Peripheral nerve regeneration: Experimental strategies and future perspectives

(2015) Alessandro Faroni et al.   ADVANCED DRUG DELIVERY REVIEWS

Graphene Potentiates the Myocardial Repair Efficacy of Mesenchymal Stem Cells by Stimulating the Expression of Angiogenic Growth Factors and Gap Junction Protein

(2015) Jooyeon Park et al.   ADVANCED FUNCTIONAL MATERIALS

In-situ birth of MSCs multicellular spheroids in poly( l -glutamic acid)/chitosan scaffold for hyaline-like cartilage regeneration

(2015) Kunxi Zhang et al.   BIOMATERIALS

Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver

(2015) Meritxell Huch et al.   CELL

Three-dimensional spheroid culture of human umbilical cord mesenchymal stem cells promotes cell yield and stemness maintenance

(2015) Yi Li et al.   CELL AND TISSUE RESEARCH

Regeneration of Thyroid Function by Transplantation of Differentiated Pluripotent Stem Cells

(2015) Anita A. Kurmann et al.   Cell Stem Cell

Microfluidic 3D cell culture: from tools to tissue models

(2015) Vincent van Duinen et al.   CURRENT OPINION IN BIOTECHNOLOGY

Three-Dimensional Cell Culture: A Breakthrough in Vivo

(2015) Delphine Antoni et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Ductal pancreatic cancer modeling and drug screening using human pluripotent stem cell– and patient-derived tumor organoids

(2015) Ling Huang et al.   NATURE MEDICINE

In vitro generation of human pluripotent stem cell derived lung organoids

(2015) Briana R Dye et al.   eLife

Drug discovery through stem cell-based organoid models

(2014) Adrian Ranga et al.   ADVANCED DRUG DELIVERY REVIEWS

Switchable Water-Adhesive, Superhydrophobic Palladium-Layered Silicon Nanowires Potentiate the Angiogenic Efficacy of Human Stem Cell Spheroids

(2014) Jungmok Seo et al.   ADVANCED MATERIALS

Multiscale patterned transplantable stem cell patches for bone tissue regeneration

(2014) Jangho Kim et al.   BIOMATERIALS

Substrate-mediated nanoparticle/gene delivery to MSC spheroids and their applications in peripheral nerve regeneration

(2014) Ting-Chen Tseng et al.   BIOMATERIALS

Mesenchymal stem cell spheroids exhibit enhanced in-vitro and in-vivo osteoregenerative potential

(2014) Yuichiro Yamaguchi et al.   BMC BIOTECHNOLOGY

Lung Stem Cell Differentiation in Mice Directed by Endothelial Cells via a BMP4-NFATc1-Thrombospondin-1 Axis

(2014) Joo-Hyeon Lee et al.   CELL

3D tumor models: history, advances and future perspectives

(2014) Parul Benien et al.   Future Oncology

Scaffold-free Prevascularized Microtissue Spheroids for Pulp Regeneration

(2014) W.L. Dissanayaka et al.   JOURNAL OF DENTAL RESEARCH

Modelling human development and disease in pluripotent stem-cell-derived gastric organoids

(2014) Kyle W. McCracken et al.   NATURE

Modulating the stem cell niche for tissue regeneration

(2014) Steven W Lane et al.   NATURE BIOTECHNOLOGY

Single Lgr5- or Lgr6-expressing taste stem/progenitor cells generate taste bud cells ex vivo

(2014) Wenwen Ren et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Formation of Well-defined Embryoid Bodies from Dissociated Human Induced Pluripotent Stem Cells using Microfabricated Cell-repellent Microwell Arrays

(2014) Giuseppe Pettinato et al.   Scientific Reports

The substrate-dependent regeneration capacity of mesenchymal stem cell spheroids derived on various biomaterial surfaces

(2014) Guo-Shiang Huang et al.   Biomaterials Science

Unlimited in vitro expansion of adult bi-potent pancreas progenitors through the Lgr5/R-spondin axis

(2013) Meritxell Huch et al.   EMBO JOURNAL

3Din vitrotissue models and their potential for drug screening

(2013) Lauren Kimlin et al.   Expert Opinion on Drug Discovery

Generation of inner ear sensory epithelia from pluripotent stem cells in 3D culture

(2013) Karl R. Koehler et al.   NATURE

In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration

(2013) Meritxell Huch et al.   NATURE

Vascularized and functional human liver from an iPSC-derived organ bud transplant

(2013) Takanori Takebe et al.   NATURE

Cerebral organoids model human brain development and microcephaly

(2013) Madeline A. Lancaster et al.   NATURE

Directed differentiation of human pluripotent cells to ureteric bud kidney progenitor-like cells

(2013) Yun Xia et al.   NATURE CELL BIOLOGY

A fully defined and scalable 3D culture system for human pluripotent stem cell expansion and differentiation

(2013) Y. Lei et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Spheroid culture as a tool for creating 3D complex tissues

(2013) Eelco Fennema et al.   TRENDS IN BIOTECHNOLOGY

Development of a valve-based cell printer for the formation of human embryonic stem cell spheroid aggregates

(2013) Alan Faulkner-Jones et al.   Biofabrication

Establishment of a Novel Lingual Organoid Culture System: Generation of Organoids Having Mature Keratinized Epithelium from Adult Epithelial Stem Cells

(2013) Hiroko Hisha et al.   Scientific Reports

Kidney Protection and Regeneration Following Acute Injury: Progress Through Stem Cell Therapy

(2012) Florian E. Tögel et al.   AMERICAN JOURNAL OF KIDNEY DISEASES

Self-Formation of Optic Cups and Storable Stratified Neural Retina from Human ESCs

(2012) Tokushige Nakano et al.   Cell Stem Cell

Three-dimensional cell culture: the missing link in drug discovery

(2012) Susan Breslin et al.   DRUG DISCOVERY TODAY

Unlike Bone, Cartilage Regeneration Remains Elusive

(2012) D. J. Huey et al.   SCIENCE

Transplantation of Cord Blood Mesenchymal Stem Cells as Spheroids Enhances Vascularization

(2012) Suk Ho Bhang et al.   TISSUE ENGINEERING PART A

Maleimide Cross-Linked Bioactive PEG Hydrogel Exhibits Improved Reaction Kinetics and Cross-Linking for Cell Encapsulation and In Situ Delivery

(2011) Edward A. Phelps et al.   ADVANCED MATERIALS

From 3D cell culture to organs-on-chips

(2011) Dongeun Huh et al.   TRENDS IN CELL BIOLOGY

High-throughput 3D spheroid culture and drug testing using a 384 hanging drop array

(2010) Yi-Chung Tung et al.   ANALYST

Lgr5+ve Stem Cells Drive Self-Renewal in the Stomach and Build Long-Lived Gastric Units In Vitro

(2010) Nick Barker et al.   Cell Stem Cell

Biomaterials and their potential applications for dental tissue engineering

(2010) Kerstin M. Galler et al.   JOURNAL OF MATERIALS CHEMISTRY

Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro

(2010) Jason R. Spence et al.   NATURE

Three-dimensional tissue culture based on magnetic cell levitation

(2010) Glauco R. Souza et al.   Nature Nanotechnology

Organotypic 3D cell culture models: using the rotating wall vessel to study host–pathogen interactions

(2010) Jennifer Barrila et al.   NATURE REVIEWS MICROBIOLOGY

Potential and Bottlenecks of Bioreactors in 3D Cell Culture and Tissue Manufacturing

(2009) David Wendt et al.   ADVANCED MATERIALS

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

(2009) Toshiro Sato et al.   NATURE

Recent advances in three-dimensional multicellular spheroid culture for biomedical research

(2008) Ruei-Zhen Lin et al.   Biotechnology Journal

Stem Cell Trafficking in Tissue Development, Growth, and Disease

(2008) Diana J. Laird et al.   CELL

Self-Organized Formation of Polarized Cortical Tissues from ESCs and Its Active Manipulation by Extrinsic Signals

(2008) Mototsugu Eiraku et al.   Cell Stem Cell

Stem Cell Therapy for Liver Disease: Parameters Governing the Success of Using Bone Marrow Mesenchymal Stem Cells

(2008) Tom K. Kuo et al.   GASTROENTEROLOGY