Fixation can change the appearance of phase separation in living cells

Transcription activation is enhanced by multivalent interactions independent of phase separation

(2022) Jorge Trojanowski et al.   MOLECULAR CELL

Tuning levels of low-complexity domain interactions to modulate endogenous oncogenic transcription

(2022) Shasha Chong et al.   MOLECULAR CELL

Evaluation of chromatin mesoscale organization

(2022) Dana Lorber et al.   APL Bioengineering

Targeting androgen receptor phase separation to overcome antiandrogen resistance

(2022) Jingjing Xie et al.   Nature Chemical Biology

Recovering mixtures of fast-diffusing states from short single-particle trajectories

(2022) Alec Heckert et al.   eLife

Single molecule microscopy reveals key physical features of repair foci in living cells

(2021) Judith Miné-Hattab et al.   eLife

Translocation of chromatin proteins to nucleoli—The influence of protein dynamics on post‐fixation localization

(2021) Mirosław Zarębski et al.   CYTOMETRY PART A

The oncogenic transcription factor FUS-CHOP can undergo nuclear liquid–liquid phase separation

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Using quantitative reconstitution to investigate multicomponent condensates

(2021) Simon L. Currie et al.   RNA

Physical observables to determine the nature of membrane-less cellular sub-compartments

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G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules

(2020) Peiguo Yang et al.   CELL

Composition-dependent thermodynamics of intracellular phase separation

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Clusters of bacterial RNA polymerase are biomolecular condensates that assemble through liquid–liquid phase separation

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Coacervates as models of membraneless organelles

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Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates

(2019) Simon Alberti et al.   CELL

Evidence for DNA-mediated nuclear compartmentalization distinct from phase separation

(2019) David Trombley McSwiggen et al.   eLife

Pol II phosphorylation regulates a switch between transcriptional and splicing condensates

(2019) Yang Eric Guo et al.   NATURE

Evaluating phase separation in live cells: diagnosis, caveats, and functional consequences

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A Solid-State Conceptualization of Information Transfer from Gene to Message to Protein

(2018) Masato Kato et al.   Annual Review of Biochemistry

A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins

(2018) Jie Wang et al.   CELL

NMR analyses on N-hydroxymethylated nucleobases – implications for formaldehyde toxicity and nucleic acid demethylases

(2018) S. Shishodia et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Imaging dynamic and selective low-complexity domain interactions that control gene transcription

(2018) Shasha Chong et al.   SCIENCE

Protein Phase Separation: A New Phase in Cell Biology

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Robust model-based analysis of single-particle tracking experiments with Spot-On

(2018) Anders S Hansen et al.   eLife

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Transcription Factors Activate Genes through the Phase-Separation Capacity of Their Activation Domains

(2018) Ann Boija et al.   CELL

Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds

(2018) Dan Bracha et al.   CELL

Biomolecular condensates: organizers of cellular biochemistry

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ATP as a biological hydrotrope

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Liquid phase condensation in cell physiology and disease

(2017) Yongdae Shin et al.   SCIENCE

Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS

(2017) Luca Lanzanò et al.   Nature Communications

CTCF and cohesin regulate chromatin loop stability with distinct dynamics

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Phase separation in biology; functional organization of a higher order

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Bright photoactivatable fluorophores for single-molecule imaging

(2016) Jonathan B Grimm et al.   NATURE METHODS

Human mutations affect the epigenetic/bookmarking function of HNF1B

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A dynamic mode of mitotic bookmarking by transcription factors

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Critical importance of appropriate fixation conditions for faithful imaging of receptor microclusters

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Formaldehyde Crosslinking: A Tool for the Study of Chromatin Complexes

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Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles

(2015) Timothy J. Nott et al.   MOLECULAR CELL

RNA transcription modulates phase transition-driven nuclear body assembly

(2015) Joel Berry et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

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Probing the target search of DNA-binding proteins in mammalian cells using TetR as model searcher

(2015) Davide Normanno et al.   Nature Communications

Liquid demixing of intrinsically disordered proteins is seeded by poly(ADP-ribose)

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Supramolecular Protein Assemblies in the Nucleus of Human Cells

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Liquid-Liquid Phase Separation in Biology

(2014) Anthony A. Hyman et al.   Annual Review of Cell and Developmental Biology

In vivo formaldehyde cross-linking: it is time for black box analysis

(2014) Alexey Gavrilov et al.   Briefings in Functional Genomics

Measuring Chromatin Interaction Dynamics on the Second Time Scale at Single-Copy Genes

(2013) K. Poorey et al.   SCIENCE

Phase transitions in the assembly of multivalent signalling proteins

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Immunolabeling artifacts and the need for live-cell imaging

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Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

Assessing the Tendency of Fluorescent Proteins to Oligomerize Under Physiologic Conditions

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Peptide–nucleotide microdroplets as a step towards a membrane-free protocell model

(2011) Shogo Koga et al.   Nature Chemistry

A Guide to Simple and Informative Binding Assays

(2010) Thomas D. Pollard   MOLECULAR BIOLOGY OF THE CELL

A single fixation protocol for proteome-wide immunofluorescence localization studies

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A Temporal Threshold for Formaldehyde Crosslinking and Fixation

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Utility of formaldehyde cross-linking and mass spectrometry in the study of protein–protein interactions

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Dynamic multiple-target tracing to probe spatiotemporal cartography of cell membranes

(2008) Arnauld Sergé et al.   NATURE METHODS

Highly inclined thin illumination enables clear single-molecule imaging in cells

(2008) Makio Tokunaga et al.   NATURE METHODS

Agonist-mediated docking of androgen receptor onto the mitotic chromatin platform discriminates intrinsic mode of action of prostate cancer drugs

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