Coacervates as models of membraneless organelles

Reversible pH-Responsive Coacervate Formation in Lipid Vesicles Activates Dormant Enzymatic Reactions

(2020) Celina Love et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Multiphase Complex Coacervate Droplets

(2020) Tiemei Lu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Nonspherical Coacervate Shapes in an Enzyme-Driven Active System

(2020) Willem Kasper Spoelstra et al.   LANGMUIR

Proteome-wide observation of the phenomenon of life on the edge of solubility

(2020) Giulia Vecchi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Valence and patterning of aromatic residues determine the phase behavior of prion-like domains

(2020) Erik W. Martin et al.   SCIENCE

Phase separation provides a mechanism to reduce noise in cells

(2020) A. Klosin et al.   SCIENCE

SPLIT: Stable Protein Coacervation Using a Light Induced Transition

(2020) Ellen H. Reed et al.   ACS Synthetic Biology

pH-Controlled Coacervate–Membrane Interactions within Liposomes

(2020) Mart G. F. Last et al.   ACS Nano

Supramolecular Nanoscaffolds within Cytomimetic Protocells as Signal Localization Hubs

(2020) Eva Magdalena Estirado et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

The Wide World of Coacervates: From the Sea to Neurodegeneration

(2020) Emanuele Astoricchio et al.   TRENDS IN BIOCHEMICAL SCIENCES

Fission and Internal Fusion of Protocell with Membraneless “Organelles” Formed by Liquid–Liquid Phase Separation

(2020) Hairong Jing et al.   LANGMUIR

De novo engineering of intracellular condensates using artificial disordered proteins

(2020) Michael Dzuricky et al.   Nature Chemistry

Lipid sponge droplets as programmable synthetic organelles

(2020) Ahanjit Bhattacharya et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Liquid–Liquid Phase Separation in Crowded Environments

(2020) Alain A. M. André et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

The nucleolus as a multiphase liquid condensate

(2020) Denis L. J. Lafontaine et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Tunable multiphase dynamics of arginine and lysine liquid condensates

(2020) Rachel S. Fisher et al.   Nature Communications

Active coacervate droplets as a model for membraneless organelles and protocells

(2020) Carsten Donau et al.   Nature Communications

Prebiotically-relevant low polyion multivalency can improve functionality of membraneless compartments

(2020) Fatma Pir Cakmak et al.   Nature Communications

Lower Critical Solution Temperature in Polyelectrolyte Complex Coacervates

(2019) Samim Ali et al.   ACS Macro Letters

Template-directed RNA polymerization and enhanced ribozyme catalysis inside membraneless compartments formed by coacervates

(2019) Raghav R. Poudyal et al.   Nature Communications

Matter over mind: Liquid phase separation and neurodegeneration

(2019) Shana Elbaum-Garfinkle   JOURNAL OF BIOLOGICAL CHEMISTRY

Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates

(2019) Simon Alberti et al.   CELL

Dynamic synthetic cells based on liquid‐liquid phase separation

(2019) Nicolas Martin   CHEMBIOCHEM

Physicochemical Characterization of Polymer‐stabilized Coacervate Protocells

(2019) N. Amy Yewdall et al.   CHEMBIOCHEM

Spontaneous driving forces give rise to protein−RNA condensates with coexisting phases and complex material properties

(2019) Steven Boeynaems et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Upper Critical Solution Temperature (UCST) Behavior of Coacervate of Cationic Protamine and Multivalent Anions

(2019) Hyungbin Kim et al.   Polymers

Engineered Ribonucleoprotein Granules Inhibit Translation in Protocells

(2019) Joseph R. Simon et al.   MOLECULAR CELL

Spatial Organization in Proteinaceous Membrane‐Stabilized Coacervate Protocells

(2019) Junbo Li et al.   Small

Spatiotemporal control of coacervate formation within liposomes

(2019) Siddharth Deshpande et al.   Nature Communications

Mimicking Cellular Compartmentalization in a Hierarchical Protocell through Spontaneous Spatial Organization

(2019) Alexander F. Mason et al.   ACS Central Science

Liquid–Liquid Phase Separation in Disease

(2019) Simon Alberti et al.   Annual Review of Genetics

Charge-Based Separation of Proteins Using Polyelectrolyte Complexes as Models for Membraneless Organelles

(2019) Jéré J. van Lente et al.   BIOMACROMOLECULES

Photoswitchable Phase Separation and Oligonucleotide Trafficking in DNA Coacervate Microdroplets

(2019) Nicolas Martin et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Formation of Multiphase Complex Coacervates and Partitioning of Biomolecules within them

(2019) Gregory A. Mountain et al.   BIOMACROMOLECULES

Quantifying Dynamics in Phase-Separated Condensates Using Fluorescence Recovery after Photobleaching

(2019) Nicole O. Taylor et al.   BIOPHYSICAL JOURNAL

Ion Content of Polyelectrolyte Complex Coacervates and the Donnan Equilibrium

(2019) Joseph B. Schlenoff et al.   MACROMOLECULES

Properties of Stress Granule and P-Body Proteomes

(2019) Ji-Young Youn et al.   MOLECULAR CELL

Intermolecular Charge-Transfer Modulates Liquid–Liquid Phase Separation and Liquid-to-Solid Maturation of an Intrinsically Disordered pH-Responsive Domain

(2019) Priyanka Dogra et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Probing and engineering liquid-phase organelles

(2019) Dan Bracha et al.   NATURE BIOTECHNOLOGY

Physical Principles and Extant Biology Reveal Roles for RNA-Containing Membraneless Compartments in Origins of Life Chemistry

(2018) Raghav R. Poudyal et al.   BIOCHEMISTRY

FUS Phase Separation Is Modulated by a Molecular Chaperone and Methylation of Arginine Cation-π Interactions

(2018) Seema Qamar et al.   CELL

Oligonucleotide–Peptide Complexes: Phase Control by Hybridization

(2018) Jeffrey R. Vieregg et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Stress granule formation via ATP depletion-triggered phase separation

(2018) Jean David Wurtz et al.   NEW JOURNAL OF PHYSICS

Reversible generation of coacervate droplets in an enzymatic network

(2018) Karina K. Nakashima et al.   Soft Matter

Membraneless Compartmentalization Facilitates Enzymatic Cascade Reactions and Reduces Substrate Inhibition

(2018) Taisuke Kojima et al.   ACS Applied Materials & Interfaces

Multifunctional Protein Materials and Microreactors using Low Complexity Domains as Molecular Adhesives

(2018) Lenka Faltova et al.   ACS Nano

Temperature-Dependent Complex Coacervation of Engineered Elastin-like Polypeptide and Hyaluronic Acid Polyelectrolytes

(2018) James D. Tang et al.   BIOMACROMOLECULES

The P Granules of C. elegans: A Genetic Model for the Study of RNA–Protein Condensates

(2018) Geraldine Seydoux   JOURNAL OF MOLECULAR BIOLOGY

Methods for Physical Characterization of Phase-Separated Bodies and Membrane-less Organelles

(2018) Diana M. Mitrea et al.   JOURNAL OF MOLECULAR BIOLOGY

Electric field-induced circulation and vacuolization regulate enzyme reactions in coacervate-based protocells

(2018) Yudan Yin et al.   Soft Matter

Compartmentalised RNA catalysis in membrane-free coacervate protocells

(2018) Björn Drobot et al.   Nature Communications

Controllable protein phase separation and modular recruitment to form responsive membraneless organelles

(2018) Benjamin S. Schuster et al.   Nature Communications

Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds

(2018) Dan Bracha et al.   CELL

Membraneless nuclear organelles and the search for phases within phases

(2018) Iain A. Sawyer et al.   Wiley Interdisciplinary Reviews-RNA

Hierarchical Self-Assembly of a Copolymer-Stabilized Coacervate Protocell

(2017) Alexander F. Mason et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

P-Body Purification Reveals the Condensation of Repressed mRNA Regulons

(2017) Arnaud Hubstenberger et al.   MOLECULAR CELL

Phase behaviour of disordered proteins underlying low density and high permeability of liquid organelles

(2017) Ming-Tzo Wei et al.   Nature Chemistry

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Structural and hydrodynamic properties of an intrinsically disordered region of a germ cell-specific protein on phase separation

(2017) Jacob P. Brady et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rheological characterization of liquid-to-solid transitions in bulk polyelectrolyte complexes

(2017) Yalin Liu et al.   Soft Matter

Aberrant Compartment Formation by HSPB2 Mislocalizes Lamin A and Compromises Nuclear Integrity and Function

(2017) Federica F. Morelli et al.   Cell Reports

Effects of oxidative and thermal stresses on stress granule formation in human induced pluripotent stem cells

(2017) Freshteh Palangi et al.   PLoS One

Compositional Control of Phase-Separated Cellular Bodies

(2016) Salman F. Banani et al.   CELL

Coexisting Liquid Phases Underlie Nucleolar Subcompartments

(2016) Marina Feric et al.   CELL

The physics of protein self-assembly

(2016) Jennifer J. McManus et al.   CURRENT OPINION IN COLLOID & INTERFACE SCIENCE

Polyamine/Nucleotide Coacervates Provide Strong Compartmentalization of Mg2+, Nucleotides, and RNA

(2016) Erica A. Frankel et al.   LANGMUIR

RNA-Based Coacervates as a Model for Membraneless Organelles: Formation, Properties, and Interfacial Liposome Assembly

(2016) William M. Aumiller et al.   LANGMUIR

Selective Uptake and Refolding of Globular Proteins in Coacervate Microdroplets

(2016) Nicolas Martin et al.   LANGMUIR

Sequence Determinants of Intracellular Phase Separation by Complex Coacervation of a Disordered Protein

(2016) Chi W. Pak et al.   MOLECULAR CELL

Membraneless organelles can melt nucleic acid duplexes and act as biomolecular filters

(2016) Timothy J. Nott et al.   Nature Chemistry

Growth and division of active droplets provides a model for protocells

(2016) David Zwicker et al.   Nature Physics

Sequence-Specific Polyampholyte Phase Separation in Membraneless Organelles

(2016) Yi-Hsuan Lin et al.   PHYSICAL REVIEW LETTERS

Complex coacervation of supercharged proteins with polyelectrolytes

(2016) Allie C. Obermeyer et al.   Soft Matter

Phase Separation by Low Complexity Domains Promotes Stress Granule Assembly and Drives Pathological Fibrillization

(2015) Amandine Molliex et al.   CELL

Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles

(2015) Timothy J. Nott et al.   MOLECULAR CELL

Phosphorylation-mediated RNA/peptide complex coacervation as a model for intracellular liquid organelles

(2015) William M. Aumiller et al.   Nature Chemistry

Rational design of functional and tunable oscillating enzymatic networks

(2015) Sergey N. Semenov et al.   Nature Chemistry

The Polyelectrolyte Complex/Coacervate Continuum

(2014) Qifeng Wang et al.   MACROMOLECULES

Linear Viscoelasticity of Polyelectrolyte Complex Coacervates

(2013) Evan Spruijt et al.   MACROMOLECULES

Conformations of intrinsically disordered proteins are influenced by linear sequence distributions of oppositely charged residues

(2013) R. K. Das et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cell-free Formation of RNA Granules: Low Complexity Sequence Domains Form Dynamic Fibers within Hydrogels

(2012) Masato Kato et al.   CELL

Phase transitions in the assembly of multivalent signalling proteins

(2012) Pilong Li et al.   NATURE

Peptide–nucleotide microdroplets as a step towards a membrane-free protocell model

(2011) Shogo Koga et al.   Nature Chemistry

Soft active aggregates: mechanics, dynamics and self-assembly of liquid-like intracellular protein bodies

(2011) Clifford P. Brangwynne   Soft Matter

Binodal Compositions of Polyelectrolyte Complexes

(2010) Evan Spruijt et al.   MACROMOLECULES

Relaxation Dynamics at Different Time Scales in Electrostatic Complexes: Time-Salt Superposition

(2010) Evan Spruijt et al.   PHYSICAL REVIEW LETTERS

Local Water Dynamics in Coacervated Polyelectrolytes Monitored through Dynamic Nuclear Polarization-Enhanced1H NMR

(2009) Ravinath Kausik et al.   MACROMOLECULES

Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation

(2009) C. P. Brangwynne et al.   SCIENCE

Stress granules: the Tao of RNA triage

(2008) Paul Anderson et al.   TRENDS IN BIOCHEMICAL SCIENCES