Using quantitative reconstitution to investigate multicomponent condensates

Structure–Function Properties in Disordered Condensates

(2021) Kamal Bhandari et al.   JOURNAL OF PHYSICAL CHEMISTRY B

High-fidelity reconstitution of stress granules and nucleoli in mammalian cellular lysate

(2021) Brian D. Freibaum et al.   JOURNAL OF CELL BIOLOGY

Designer Condensates: A Toolkit for the Biomolecular Architect

(2021) Renee L. Hastings et al.   JOURNAL OF MOLECULAR BIOLOGY

Biomolecular condensates at the nexus of cellular stress, protein aggregation disease and ageing

(2021) Simon Alberti et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Ligand effects on phase separation of multivalent macromolecules

(2021) Kiersten M. Ruff et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Biomolecular condensates amplify mRNA decapping by biasing enzyme conformation

(2021) Ryan W. Tibble et al.   Nature Chemical Biology

Roles of liquid–liquid phase separation in bacterial RNA metabolism

(2021) Vidhyadhar Nandana et al.   CURRENT OPINION IN MICROBIOLOGY

Generic nature of the condensed states of proteins

(2021) Monika Fuxreiter et al.   NATURE CELL BIOLOGY

Mechanistic dissection of increased enzymatic rate in a phase-separated compartment

(2021) William Peeples et al.   Nature Chemical Biology

Liquid-like chromatin in the cell: What can we learn from imaging and computational modeling?

(2021) Yuji Itoh et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Physical Principles Underlying the Complex Biology of Intracellular Phase Transitions

(2020) Jeong-Mo Choi et al.   Annual Review of Biophysics

Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization

(2020) David W. Sanders et al.   CELL

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules

(2020) Peiguo Yang et al.   CELL

RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation

(2020) Jordina Guillén-Boixet et al.   CELL

Composition-dependent thermodynamics of intracellular phase separation

(2020) Joshua A. Riback et al.   NATURE

Liquid network connectivity regulates the stability and composition of biomolecular condensates with many components

(2020) Jorge R. Espinosa et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Analysis of biomolecular condensates and protein phase separation with microfluidic technology

(2020) Miriam Linsenmeier et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

An In Vitro Assembly System Identifies Roles for RNA Nucleation and ATP in Yeast Stress Granule Formation

(2020) Kyle Begovich et al.   MOLECULAR CELL

RNA contributions to the form and function of biomolecular condensates

(2020) Christine Roden et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Phase transition of RNA−protein complexes into ordered hollow condensates

(2020) Ibraheem Alshareedah et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Pat1 increases the range of decay factors and RNA bound by the Lsm1-7 complex

(2020) Joseph H Lobel et al.   RNA

Biomolecular Condensates in the Nucleus

(2020) Benjamin R. Sabari et al.   TRENDS IN BIOCHEMICAL SCIENCES

A quantitative inventory of yeast P body proteins reveals principles of composition and specificity

(2020) Wenmin Xing et al.   eLife

Pat1 promotes processing body assembly by enhancing the phase separation of the DEAD-box ATPase Dhh1 and RNA

(2019) Ruchika Sachdev et al.   eLife

A molecular assembly phase transition and kinetic proofreading modulate Ras activation by SOS

(2019) William Y. C. Huang et al.   SCIENCE

Stoichiometry controls activity of phase-separated clusters of actin signaling proteins

(2019) Lindsay B. Case et al.   SCIENCE

Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates

(2019) Simon Alberti et al.   CELL

Regulation of Transmembrane Signaling by Phase Separation

(2019) Lindsay B. Case et al.   Annual Review of Biophysics

Evidence for DNA-mediated nuclear compartmentalization distinct from phase separation

(2019) David Trombley McSwiggen et al.   eLife

A composition-dependent molecular clutch between T cell signaling condensates and actin

(2019) Jonathon A Ditlev et al.   eLife

DEAD-box ATPases are global regulators of phase-separated organelles

(2019) Maria Hondele et al.   NATURE

Label-Free Analysis of Protein Aggregation and Phase Behavior

(2019) Zenon Toprakcioglu et al.   ACS Nano

Organization of Chromatin by Intrinsic and Regulated Phase Separation

(2019) Bryan A. Gibson et al.   CELL

Evaluating phase separation in live cells: diagnosis, caveats, and functional consequences

(2019) David T. McSwiggen et al.   GENES & DEVELOPMENT

PhaSepDB: a database of liquid–liquid phase separation related proteins

(2019) Kaiqiang You et al.   NUCLEIC ACIDS RESEARCH

PhaSePro: the database of proteins driving liquid–liquid phase separation

(2019) Bálint Mészáros et al.   NUCLEIC ACIDS RESEARCH

Learning the pattern of epistasis linking genotype and phenotype in a protein

(2019) Frank J. Poelwijk et al.   Nature Communications

LASSI: A lattice model for simulating phase transitions of multivalent proteins

(2019) Jeong-Mo Choi et al.   PLoS Computational Biology

GC content shapes mRNA storage and decay in human cells

(2019) Maïté Courel et al.   eLife

Theories for Sequence-Dependent Phase Behaviors of Biomolecular Condensates

(2018) Yi-Hsuan Lin et al.   BIOCHEMISTRY

Context-Dependent and Disease-Specific Diversity in Protein Interactions within Stress Granules

(2018) Sebastian Markmiller et al.   CELL

High-Density Proximity Mapping Reveals the Subcellular Organization of mRNA-Associated Granules and Bodies

(2018) Ji-Young Youn et al.   MOLECULAR CELL

RNA self-assembly contributes to stress granule formation and defining the stress granule transcriptome

(2018) Briana Van Treeck et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

RNA buffers the phase separation behavior of prion-like RNA binding proteins

(2018) Shovamayee Maharana et al.   SCIENCE

Intrinsically Disordered Regions Can Contribute Promiscuous Interactions to RNP Granule Assembly

(2018) David S.W. Protter et al.   Cell Reports

Context-dependent deposition and regulation of mRNAs in P-bodies

(2018) Congwei Wang et al.   eLife

P-Body Purification Reveals the Condensation of Repressed mRNA Regulons

(2017) Arnaud Hubstenberger et al.   MOLECULAR CELL

The Stress Granule Transcriptome Reveals Principles of mRNA Accumulation in Stress Granules

(2017) Anthony Khong et al.   MOLECULAR CELL

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

A synergistic network of interactions promotes the formation of in vitro processing bodies and protects mRNA against decapping

(2017) Stefan Schütz et al.   NUCLEIC ACIDS RESEARCH

Numerous interactions act redundantly to assemble a tunable size of P bodies inSaccharomyces cerevisiae

(2017) Bhalchandra S. Rao et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

ATPase-Modulated Stress Granules Contain a Diverse Proteome and Substructure

(2016) Saumya Jain et al.   CELL

Compositional Control of Phase-Separated Cellular Bodies

(2016) Salman F. Banani et al.   CELL

The Activity-Dependent Regulation of Protein Kinase Stability by the Localization to P-Bodies

(2016) B. Zhang et al.   GENETICS

Sequence Determinants of Intracellular Phase Separation by Complex Coacervation of a Disordered Protein

(2016) Chi W. Pak et al.   MOLECULAR CELL

Principles and Properties of Stress Granules

(2016) David S.W. Protter et al.   TRENDS IN CELL BIOLOGY

ATPase activity of the DEAD-box protein Dhh1 controls processing body formation

(2016) Christopher Frederick Mugler et al.   eLife

RNA Controls PolyQ Protein Phase Transitions

(2015) Huaiying Zhang et al.   MOLECULAR CELL

Formation and Maturation of Phase-Separated Liquid Droplets by RNA-Binding Proteins

(2015) Yuan Lin et al.   MOLECULAR CELL

The disordered P granule protein LAF-1 drives phase separation into droplets with tunable viscosity and dynamics

(2015) Shana Elbaum-Garfinkle et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In Vitro Reconstitution of a Cellular Phase-Transition Process that Involves the mRNA Decapping Machinery

(2014) Simon A. Fromm et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Liquid-Liquid Phase Separation in Biology

(2014) Anthony A. Hyman et al.   Annual Review of Cell and Developmental Biology

Enzyme clustering accelerates processing of intermediates through metabolic channeling

(2014) Michele Castellana et al.   NATURE BIOTECHNOLOGY

Eukaryotic Stress Granules Are Cleared by Autophagy and Cdc48/VCP Function

(2013) J. Ross Buchan et al.   CELL

Cell-free Formation of RNA Granules: Low Complexity Sequence Domains Form Dynamic Fibers within Hydrogels

(2012) Masato Kato et al.   CELL

RNA Degradation in Saccharomyces cerevisae

(2012) Roy Parker   GENETICS

Phase transitions in the assembly of multivalent signalling proteins

(2012) Pilong Li et al.   NATURE

P-Bodies and Stress Granules: Possible Roles in the Control of Translation and mRNA Degradation

(2012) C. J. Decker et al.   Cold Spring Harbor Perspectives in Biology

Coupled 5′ Nucleotide Recognition and Processivity in Xrn1-Mediated mRNA Decay

(2011) Martin Jinek et al.   MOLECULAR CELL

From unwinding to clamping — the DEAD box RNA helicase family

(2011) Patrick Linder et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Decapping Activators in Saccharomyces cerevisiae Act by Multiple Mechanisms

(2010) Tracy Nissan et al.   MOLECULAR CELL

The Nucleus Introduced

(2010) T. Pederson   Cold Spring Harbor Perspectives in Biology

The Nucleolus

(2010) T. Pederson   Cold Spring Harbor Perspectives in Biology

P bodies promote stress granule assembly inSaccharomyces cerevisiae

(2008) J. Ross Buchan et al.   JOURNAL OF CELL BIOLOGY