Engineering the enzyme toolbox to tailor glycosylation in small molecule natural products and protein biologics

Ex vivo enzymatic treatment converts blood type A donor lungs into universal blood type lungs

(2022) Aizhou Wang et al.   Science Translational Medicine

Functional and Structural Dissection of a Plant Steroid 3-O-Glycosyltransferase Facilitated the Engineering Enhancement of Sugar Donor Promiscuity

(2022) Wei Huang et al.   ACS Catalysis

Immunobiology of Carbohydrates: Implications for Novel Vaccine and Adjuvant Design Against Infectious Diseases

(2022) Giuseppe Stefanetti et al.   Frontiers in Cellular and Infection Microbiology

Author Correction: Cycloalkane-modified amphiphilic polymers provide direct extraction of membrane proteins for CryoEM analysis

(2022) Anna J. Higgins et al.   Communications Biology

Carbohydrate-active enzymes (CAZymes) in the gut microbiome

(2022) Jacob F. Wardman et al.   NATURE REVIEWS MICROBIOLOGY

O-/N-/S-Specificity in Glycosyltransferase Catalysis: From Mechanistic Understanding to Engineering

(2021) David Teze et al.   ACS Catalysis

Glycosyltransferase Co‐Immobilization for Natural Product Glycosylation: Cascade Biosynthesis of the C ‐Glucoside Nothofagin with Efficient Reuse of Enzymes

(2021) Hui Liu et al.   ADVANCED SYNTHESIS & CATALYSIS

Genetic glycoengineering in mammalian cells

(2021) Yoshiki Narimatsu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Biocatalytic Application of a Membrane‐Bound Coumarin C‐Glucosyltransferase in the Synthesis of Coumarin and Benzofuran C‐Glucosides

(2021) Dawei Chen et al.   ADVANCED SYNTHESIS & CATALYSIS

Carbohydrate based meningococcal vaccines: past and present overview

(2021) Francesco Berti et al.   GLYCOCONJUGATE JOURNAL

Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles

(2021) Jasmine M. Hershewe et al.   Nature Communications

Advanced glycosidases as ingenious biosynthetic instruments

(2021) Zuzana Mészáros et al.   BIOTECHNOLOGY ADVANCES

Protein C-Mannosylation and C-Mannosyl Tryptophan in Chemical Biology and Medicine

(2021) Shiho Minakata et al.   MOLECULES

Efficient O-Glycosylation of Triterpenes Enabled by Protein Engineering of Plant Glycosyltransferase UGT74AC1

(2020) Jiao Li et al.   ACS Catalysis

Supramolecular Photothermal Nanomedicine Mediated Distant Tumor Inhibition via PD-1 and TIM-3 Blockage

(2020) Tong-Yi Huang et al.   Frontiers in Chemistry

Selective N-glycan editing on living cell surfaces to probe glycoconjugate function

(2020) Feng Tang et al.   Nature Chemical Biology

Flavonoids, terpenoids, and polyketide antibiotics: Role of glycosylation and biocatalytic tactics in engineering glycosylation

(2020) Ushasree Mrudulakumari Vasudevan et al.   BIOTECHNOLOGY ADVANCES

N-glycans as functional effectors of genetic and epigenetic disease risk

(2020) Tamara Štambuk et al.   MOLECULAR ASPECTS OF MEDICINE

Global view of human protein glycosylation pathways and functions

(2020) Katrine T. Schjoldager et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

SELECT-GLYCOCIN: a recombinant microbial system for expression and high-throughput screening of glycocins

(2020) Pravinkumar Choudhary et al.   GLYCOCONJUGATE JOURNAL

Glycoengineering of Antibodies for Modulating Functions

(2019) Lai-Xi Wang et al.   Annual Review of Biochemistry

An enzymatic pathway in the human gut microbiome that converts A to universal O type blood

(2019) Peter Rahfeld et al.   Nature Microbiology

A cell-free biosynthesis platform for modular construction of protein glycosylation pathways

(2019) Weston Kightlinger et al.   Nature Communications

Toward universal donor blood: Enzymatic conversion of A and B to O type

(2019) Peter Rahfeld et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

(2019) Nagesh K. Tripathi et al.   Frontiers in Bioengineering and Biotechnology

Site-specific immobilization of endoglycosidases for streamlined chemoenzymatic glycan remodeling of antibodies

(2018) Tiezheng Li et al.   CARBOHYDRATE RESEARCH

Development of glycosynthases with broad glycan specificity for the efficient glyco-remodeling of antibodies

(2018) Sachin S. Shivatare et al.   CHEMICAL COMMUNICATIONS

Expression of recombinant proteins in insect and mammalian cells

(2018) Edward A. McKenzie et al.   METHODS

Employing a biochemical protecting group for a sustainable indigo dyeing strategy

(2018) Tammy M Hsu et al.   Nature Chemical Biology

Structural absorption by barbule microstructures of super black bird of paradise feathers

(2018) Dakota E. McCoy et al.   Nature Communications

Probing and Engineering Key Residues for Bis-C-glycosylation and Promiscuity of a C-Glycosyltransferase

(2018) Dawei Chen et al.   ACS Catalysis

Cell-free protein synthesis as a novel tool for directed glycoengineering of active erythropoietin

(2018) Anne Zemella et al.   Scientific Reports

Rapid Discovery of Glycocins through Pathway Refactoring in Escherichia coli

(2018) Hengqian Ren et al.   ACS Chemical Biology

Biotherapeutics: Challenges and Opportunities for Predictive Toxicology of Monoclonal Antibodies

(2018) Dale Johnson   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Structural dissection of sterol glycosyltransferase UGT51 from Saccharomyces cerevisiae for substrate specificity

(2018) Liuqing Chen et al.   JOURNAL OF STRUCTURAL BIOLOGY

Double-slit photoelectron interference in strong-field ionization of the neon dimer

(2018) Maksim Kunitski et al.   Nature Communications

An iterative glycosyltransferase EntS catalyzes transfer and extension of O- and S-linked monosaccharide in enterocin 96

(2017) Rupa Nagar et al.   GLYCOBIOLOGY

Enzyme replacement therapy and beyond—in memoriam Roscoe O. Brady, M.D. (1923–2016)

(2017) Markus Ries   JOURNAL OF INHERITED METABOLIC DISEASE

Biosynthesis of plant-derived ginsenoside Rh2 in yeast via repurposing a key promiscuous microbial enzyme

(2017) Yu Zhuang et al.   METABOLIC ENGINEERING

Improving the Catalytic Property of the Glycoside Hydrolase LXYL-P1–2 by Directed Evolution

(2017) Jing-Jing Chen et al.   MOLECULES

Chemoenzymatic synthesis of glycoengineered IgG antibodies and glycosite-specific antibody–drug conjugates

(2017) Feng Tang et al.   Nature Protocols

Glycosylation engineering of therapeutic IgG antibodies: challenges for the safety, functionality and efficacy

(2017) Yusuke Mimura et al.   Protein & Cell

Glycosyltransferase cascades for natural product glycosylation: Use of plant instead of bacterial sucrose synthases improves the UDP-glucose recycling from sucrose and UDP

(2017) Alexander Gutmann et al.   Biotechnology Journal

Advances in Enzymatic Glycoside Synthesis

(2016) Phillip M. Danby et al.   ACS Chemical Biology

Optimal Synthetic Glycosylation of a Therapeutic Antibody

(2016) Thomas B. Parsons et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Enzymatic Synthesis of Acylphloroglucinol 3-C -Glucosides from 2-O -Glucosides using a C -Glycosyltransferase from Mangifera indica

(2016) Dawei Chen et al.   CHEMISTRY-A EUROPEAN JOURNAL

The glycocins: in a class of their own

(2016) Gillian E Norris et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Biological roles of glycans

(2016) Ajit Varki   GLYCOBIOLOGY

Glycosynthase Mutants of Endoglycosidase S2 Show Potent Transglycosylation Activity and Remarkably Relaxed Substrate Specificity for Antibody Glycosylation Remodeling

(2016) Tiezheng Li et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Endo-F3 Glycosynthase Mutants Enable Chemoenzymatic Synthesis of Core-fucosylated Triantennary Complex Type Glycopeptides and Glycoproteins

(2016) John P. Giddens et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

One-pot N-glycosylation remodeling of IgG with non-natural sialylglycopeptides enables glycosite-specific and dual-payload antibody–drug conjugates

(2016) Feng Tang et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Engineering a Carbohydrate-processing Transglycosidase into Glycosyltransferase for Natural Product Glycodiversification

(2016) Chaoning Liang et al.   Scientific Reports

A comprehensive review of glycosylated bacterial natural products

(2015) Sherif I. Elshahawi et al.   CHEMICAL SOCIETY REVIEWS

EndoS and EndoS2 hydrolyze Fc-glycans on therapeutic antibodies with different glycoform selectivity and can be used for rapid quantification of high-mannose glycans

(2015) Jonathan Sjögren et al.   GLYCOBIOLOGY

Sialylation of IgG Fc domain impairs complement-dependent cytotoxicity

(2015) Isaak Quast et al.   JOURNAL OF CLINICAL INVESTIGATION

Reaction Mechanisms in Carbohydrate-Active Enzymes: Glycoside Hydrolases and Glycosyltransferases. Insights from ab Initio Quantum Mechanics/Molecular Mechanics Dynamic Simulations

(2015) Albert Ardèvol et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Toward Efficient Enzymes for the Generation of Universal Blood through Structure-Guided Directed Evolution

(2015) David H. Kwan et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A common glycan structure on immunoglobulin G for enhancement of effector functions

(2015) Chin-Wei Lin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Increased efficiency of Campylobacter jejuni N-oligosaccharyltransferase PglB by structure-guided engineering

(2015) J. Ihssen et al.   Open Biology

Glycoengineered Monoclonal Antibodies with Homogeneous Glycan (M3, G0, G2, and A2) Using a Chemoenzymatic Approach Have Different Affinities for FcγRIIIa and Variable Antibody-Dependent Cellular Cytotoxicity Activities

(2015) Masaki Kurogochi et al.   PLoS One

Chemoenzymatic Fc Glycosylation via Engineered Aldehyde Tags

(2014) Elizabeth L. Smith et al.   BIOCONJUGATE CHEMISTRY

Towards the synthesis of glycosylated dihydrochalcone natural products using glycosyltransferase-catalysed cascade reactions

(2014) Alexander Gutmann et al.   GREEN CHEMISTRY

Structural Characterization of Anti-Inflammatory Immunoglobulin G Fc Proteins

(2014) Alysia A. Ahmed et al.   JOURNAL OF MOLECULAR BIOLOGY

Engineered oligosaccharyltransferases with greatly relaxed acceptor-site specificity

(2014) Anne A Ollis et al.   Nature Chemical Biology

A two-step enzymatic glycosylation of polypeptides with complex N -glycans

(2013) Joseph V. Lomino et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Synthesis of Glycans and Glycopolymers Through Engineered Enzymes

(2013) Zachary Armstrong et al.   BIOPOLYMERS

Chemoenzymatic synthesis of immunogenic meningococcal group C polysialic acid-tetanus Hc fragment glycoconjugates

(2013) Pumtiwitt C. McCarthy et al.   GLYCOCONJUGATE JOURNAL

Metabolic engineering of Saccharomyces cerevisiae for production of ginsenosides

(2013) Zhubo Dai et al.   METABOLIC ENGINEERING

Expanding the glycoengineering toolbox: the rise of bacterial N-linked protein glycosylation

(2013) Jenny L. Baker et al.   TRENDS IN BIOTECHNOLOGY

Switching betweenO- andC-Glycosyltransferase through Exchange of Active-Site Motifs

(2012) Alexander Gutmann et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Remarkable Transglycosylation Activity of Glycosynthase Mutants of Endo-D, an Endo-β-N-acetylglucosaminidase fromStreptococcus pneumoniae

(2012) Shu-Quan Fan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Chemoenzymatic Glycoengineering of Intact IgG Antibodies for Gain of Functions

(2012) Wei Huang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Probing the Regiospecificity of Enzyme-Catalyzed Steroid Glycosylation

(2012) Maoquan Zhou et al.   ORGANIC LETTERS

Structural, Dynamic, and Chemical Characterization of a Novel S-Glycosylated Bacteriocin

(2011) Hariprasad Venugopal et al.   BIOCHEMISTRY

Bioactivities of Glycoalkaloids and Their Aglycones from Solanum Species

(2011) Sinead Eileen Milner et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Characterization and Acceptor Preference of a Soluble Meningococcal Group C Polysialyltransferase

(2011) D. C. Peterson et al.   JOURNAL OF BACTERIOLOGY

Expanding the Nucleotide and Sugar 1-Phosphate Promiscuity of Nucleotidyltransferase RmlA via Directed Evolution

(2011) Rocco Moretti et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Toward Efficient Enzymatic Glycan Synthesis: Directed Evolution and Enzyme Engineering

(2011) David H. Kwan et al.   JOURNAL OF CARBOHYDRATE CHEMISTRY

Substrate Selectivity of the Sublancin S-Glycosyltransferase

(2011) Huan Wang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Chemoenzymatic Synthesis and Fcγ Receptor Binding of Homogeneous Glycoforms of Antibody Fc Domain. Presence of a Bisecting Sugar Moiety Enhances the Affinity of Fc to FcγIIIa Receptor

(2011) Guozhang Zou et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Sublancin is not a lantibiotic but an S-linked glycopeptide

(2011) Trent J Oman et al.   Nature Chemical Biology

Site-specific enzymatic polysialylation of therapeutic proteins using bacterial enzymes

(2011) T. Lindhout et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Production of glycoprotein vaccines in Escherichia coli

(2010) Julian Ihssen et al.   Microbial Cell Factories

Chimeric Glycosyltransferases for the Generation of Hybrid Glycopeptides

(2009) Andrew W. Truman et al.   CHEMISTRY & BIOLOGY

Efficient Glycosynthase Mutant Derived fromMucor hiemalisEndo-β-N-acetylglucosaminidase Capable of Transferring Oligosaccharide from Both Sugar Oxazoline and NaturalN-Glycan

(2009) Midori Umekawa et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Engineering complex-type N-glycosylation in Pichia pastoris using GlycoSwitch technology

(2009) Pieter P Jacobs et al.   Nature Protocols

Probing the Aglycon Promiscuity of an Engineered Glycosyltransferase

(2008) Richard W. Gantt et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

The Glycosylphosphatidylinositol Anchor: A Complex Membrane-Anchoring Structure for Proteins†

(2008) Margot G. Paulick et al.   BIOCHEMISTRY

Glycoengineering of Human IgG1-Fc through Combined Yeast Expression andin VitroChemoenzymatic Glycosylation†§

(2008) Yadong Wei et al.   BIOCHEMISTRY

Safety-Related Regulatory Actions for Biologicals Approved in the United States and the European Union

(2008)   JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION

Glycosyltransferases: Structures, Functions, and Mechanisms

(2007) L.L. Lairson et al.   Annual Review of Biochemistry