Development of glycosynthases with broad glycan specificity for the efficient glyco-remodeling of antibodies

Glycoengineering of antibody (Herceptin) through yeast expression and in vitro enzymatic glycosylation

(2018) Chiu-Ping Liu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Glyco-Engineering of Human IgG-Fc to Modulate Biologic Activities

(2016) Roy Jefferis   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

N-glycosylation heterogeneity and the influence on structure, function and pharmacokinetics of monoclonal antibodies and Fc fusion proteins

(2016) Fabian Higel et al.   EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS

Glycosynthase Mutants of Endoglycosidase S2 Show Potent Transglycosylation Activity and Remarkably Relaxed Substrate Specificity for Antibody Glycosylation Remodeling

(2016) Tiezheng Li et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Endo-F3 Glycosynthase Mutants Enable Chemoenzymatic Synthesis of Core-fucosylated Triantennary Complex Type Glycopeptides and Glycoproteins

(2016) John P. Giddens et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Modular synthesis of N-glycans and arrays for the hetero-ligand binding analysis of HIV antibodies

(2016) Sachin S. Shivatare et al.   Nature Chemistry

Using glyco-engineering to produce therapeutic proteins

(2015) Martina Dicker et al.   EXPERT OPINION ON BIOLOGICAL THERAPY

Fc glycans of therapeutic antibodies as critical quality attributes

(2015) Dietmar Reusch et al.   GLYCOBIOLOGY

EndoS and EndoS2 hydrolyze Fc-glycans on therapeutic antibodies with different glycoform selectivity and can be used for rapid quantification of high-mannose glycans

(2015) Jonathan Sjögren et al.   GLYCOBIOLOGY

Antibody Glycosylation and Its Impact on the Pharmacokinetics and Pharmacodynamics of Monoclonal Antibodies and Fc-Fusion Proteins

(2015) Liming Liu   JOURNAL OF PHARMACEUTICAL SCIENCES

Evaluation of a glycoengineered monoclonal antibody via LC-MS analysis in combination with multiple enzymatic digestion

(2015) Renpeng Liu et al.   mAbs

A common glycan structure on immunoglobulin G for enhancement of effector functions

(2015) Chin-Wei Lin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Glycoengineered Monoclonal Antibodies with Homogeneous Glycan (M3, G0, G2, and A2) Using a Chemoenzymatic Approach Have Different Affinities for FcγRIIIa and Variable Antibody-Dependent Cellular Cytotoxicity Activities

(2015) Masaki Kurogochi et al.   PLoS One

Role of sialylation in the anti-inflammatory activity of intravenous immunoglobulin - F(ab′)2versus Fc sialylation

(2014) I. Schwab et al.   CLINICAL AND EXPERIMENTAL IMMUNOLOGY

Antibodies to watch in 2015

(2014) Janice M Reichert   mAbs

The therapeutic monoclonal antibody market

(2014) Dawn M Ecker et al.   mAbs

EndoS2is a unique and conserved enzyme of serotype M49 group AStreptococcusthat hydrolyses N-linked glycans on IgG and α1-acid glycoprotein

(2013) Jonathan Sjögren et al.   BIOCHEMICAL JOURNAL

Glycoengineered CD20 antibody obinutuzumab activates neutrophils and mediates phagocytosis through CD16B more efficiently than rituximab

(2013) J. Golay et al.   BLOOD

LC-MS/MS biopharmaceutical glycoanalysis: identification of desirable reference material characteristics

(2012) John E. Schiel et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Chemoenzymatic Glycoengineering of Intact IgG Antibodies for Gain of Functions

(2012) Wei Huang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Chemoenzymatic Synthesis and Fcγ Receptor Binding of Homogeneous Glycoforms of Antibody Fc Domain. Presence of a Bisecting Sugar Moiety Enhances the Affinity of Fc to FcγIIIa Receptor

(2011) Guozhang Zou et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Unique carbohydrate-carbohydrate interactions are required for high affinity binding between Fc RIII and antibodies lacking core fucose

(2011) C. Ferrara et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Efficient transfer of sialo-oligosaccharide onto proteins by combined use of a glycosynthase-like mutant of Mucor hiemalis endoglycosidase and synthetic sialo-complex-type sugar oxazoline

(2010) Midori Umekawa et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

A Novel Role for the IgG Fc Glycan: The Anti-inflammatory Activity of Sialylated IgG Fcs

(2010) Robert M. Anthony et al.   JOURNAL OF CLINICAL IMMUNOLOGY

Glycan Engineering and Production of ‘Humanized’ Glycoprotein in Yeast Cells

(2009) Yasunori Chiba et al.   BIOLOGICAL & PHARMACEUTICAL BULLETIN

Expression systems for therapeutic glycoprotein production

(2009) Yves Durocher et al.   CURRENT OPINION IN BIOTECHNOLOGY

Efficient Glycosynthase Mutant Derived fromMucor hiemalisEndo-β-N-acetylglucosaminidase Capable of Transferring Oligosaccharide from Both Sugar Oxazoline and NaturalN-Glycan

(2009) Midori Umekawa et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Glycosynthases Enable a Highly Efficient Chemoenzymatic Synthesis ofN-Glycoproteins Carrying Intact NaturalN-Glycans

(2009) Wei Huang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Glycosylation as a strategy to improve antibody-based therapeutics

(2009) Roy Jefferis   NATURE REVIEWS DRUG DISCOVERY

Glycosylation of Recombinant Antibody Therapeutics

(2005) Royston Jefferis   BIOTECHNOLOGY PROGRESS