Methods for the Assessment of NET Formation: From Neutrophil Biology to Translational Research

Versatile, do-it-yourself, low-cost spinning disk confocal microscope

(2022) Aaron R. Halpern et al.   Biomedical Optics Express

Neutrophil Extracellular Traps (NETs) and Covid-19: A new frontiers for therapeutic modality

(2022) Hayder M. Al-Kuraishy et al.   INTERNATIONAL IMMUNOPHARMACOLOGY

Histone H3 clipping is a novel signature of human neutrophil extracellular traps

(2022) Dorothea Ogmore Tilley et al.   eLife

Scrutinizing Mechanisms of the ‘Obesity Paradox in Sepsis’: Obesity Is Accompanied by Diminished Formation of Neutrophil Extracellular Traps (NETs) Due to Restricted Neutrophil–Platelet Interactions

(2021) Iwona Cichon et al.   Cells

Intravital Imaging Allows Organ-Specific Insights Into Immune Functions

(2021) Selina K. Jorch et al.   Frontiers in Cell and Developmental Biology

ELISA detection of MPO-DNA complexes in human plasma is error-prone and yields limited information on neutrophil extracellular traps formed in vivo

(2021) Hubert Hayden et al.   PLoS One

Angiotensin II triggers release of neutrophil extracellular traps, linking thromboinflammation with essential hypertension

(2021) Akrivi Chrysanthopoulou et al.   JCI Insight

A novel cell-based assay for dynamically detecting neutrophil extracellular traps-induced lung epithelial injuries

(2020) Dandan Lv et al.   EXPERIMENTAL CELL RESEARCH

Neutrophil extracellular traps released by neutrophils impair revascularization and vascular remodeling after stroke

(2020) Lijing Kang et al.   Nature Communications

Neutrophil swarming delays the growth of clusters of pathogenic fungi

(2020) Alex Hopke et al.   Nature Communications

Production of Neutrophil Extracellular Traps Contributes to the Pathogenesis of Francisella tularemia

(2020) Sivasami Pulavendran et al.   Frontiers in Immunology

Complement and tissue factor–enriched neutrophil extracellular traps are key drivers in COVID-19 immunothrombosis

(2020) Panagiotis Skendros et al.   JOURNAL OF CLINICAL INVESTIGATION

To NET or not to NET:current opinions and state of the science regarding the formation of neutrophil extracellular traps

(2019) Sebastian Boeltz et al.   CELL DEATH AND DIFFERENTIATION

Systemic levels of anti-PAD4 autoantibodies correlate with airway obstruction in cystic fibrosis

(2019) Ruchi Yadav et al.   Journal of Cystic Fibrosis

The Neutrophil’s Role During Health and Disease

(2019) Pei Xiong Liew et al.   PHYSIOLOGICAL REVIEWS

Neutrophil Extracellular Traps

(2019) Charlotte Thålin et al.   ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY

Neutrophil extracellular traps (NET) induced by different stimuli: A comparative proteomic analysis

(2019) Andrea Petretto et al.   PLoS One

Neutrophil Diversity in Health and Disease

(2019) Carlos Silvestre-Roig et al.   TRENDS IN IMMUNOLOGY

NETosis, complement, and coagulation: a triangular relationship

(2018) Cynthia M. de Bont et al.   Cellular & Molecular Immunology

Neutrophil extracellular traps are associated with disease severity and microbiota diversity in patients with chronic obstructive pulmonary disease

(2018) Alison J. Dicker et al.   JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

REDD1/Autophagy Pathway Is Associated with Neutrophil-Driven IL-1β Inflammatory Response in Active Ulcerative Colitis

(2018) Iliana Angelidou et al.   JOURNAL OF IMMUNOLOGY

Citrullinated histone H3, a biomarker of neutrophil extracellular trap formation, predicts the risk of venous thromboembolism in cancer patients

(2018) L.-M. Mauracher et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Citrullinated histone H3 as a novel prognostic blood marker in patients with advanced cancer

(2018) Charlotte Thålin et al.   PLoS One

Neutrophil: A Cell with Many Roles in Inflammation or Several Cell Types?

(2018) Carlos Rosales   Frontiers in Physiology

Chlamydia trachomatis paralyses neutrophils to evade the host innate immune response

(2018) Karthika Rajeeve et al.   Nature Microbiology

Expanding Neutrophil Horizons: New Concepts in Inflammation

(2018) Simon M. Chatfield et al.   Journal of Innate Immunity

REDD1/autophagy pathway promotes thromboinflammation and fibrosis in human systemic lupus erythematosus (SLE) through NETs decorated with tissue factor (TF) and interleukin-17A (IL-17A)

(2018) Eleni Frangou et al.   ANNALS OF THE RHEUMATIC DISEASES

A Flow Cytometry‐Based Assay for High‐Throughput Detection and Quantification of Neutrophil Extracellular Traps in Mixed Cell Populations

(2018) Olga Zharkova et al.   CYTOMETRY PART A

Validation of an enzyme-linked immunosorbent assay for the quantification of citrullinated histone H3 as a marker for neutrophil extracellular traps in human plasma

(2017) Charlotte Thålin et al.   IMMUNOLOGIC RESEARCH

A High-Throughput Real-Time Imaging Technique To Quantify NETosis and Distinguish Mechanisms of Cell Death in Human Neutrophils

(2017) Sarthak Gupta et al.   JOURNAL OF IMMUNOLOGY

In vitro induction of NETosis: Comprehensive live imaging comparison and systematic review

(2017) Tamara Hoppenbrouwers et al.   PLoS One

NETopathies? Unraveling the Dark Side of Old Diseases through Neutrophils

(2017) Alexandros Mitsios et al.   Frontiers in Immunology

Neutrophil Extracellular Traps Promote AngiogenesisHighlights

(2016) Lulwah Aldabbous et al.   ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY

NETosis markers: Quest for specific, objective, and quantitative markers

(2016) Sakiko Masuda et al.   CLINICA CHIMICA ACTA

Flow cytometry: basic principles and applications

(2016) Aysun Adan et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Granulopoiesis and granules of human neutrophils

(2016) Jack B. Cowland et al.   IMMUNOLOGICAL REVIEWS

Clearance of apoptotic neutrophils and resolution of inflammation

(2016) Mallary C. Greenlee-Wacker   IMMUNOLOGICAL REVIEWS

Neutrophil-derived chemokines on the road to immunity

(2016) Cristina Tecchio et al.   SEMINARS IN IMMUNOLOGY

Neutrophil extracellular traps are indirectly triggered by lipopolysaccharide and contribute to acute lung injury

(2016) Shuai Liu et al.   Scientific Reports

Flow cytometric assay for direct quantification of neutrophil extracellular traps in blood samples

(2015) Mathilde Gavillet et al.   AMERICAN JOURNAL OF HEMATOLOGY

Automatic determination of NET (neutrophil extracellular traps) coverage in fluorescent microscopy images

(2015) Luis Pedro Coelho et al.   BIOINFORMATICS

Neutrophil extracellular trap formation is associated with autophagy-related signalling in ANCA-associated vasculitis

(2015) S. Tang et al.   CLINICAL AND EXPERIMENTAL IMMUNOLOGY

Expression of functional tissue factor by neutrophil extracellular traps in culprit artery of acute myocardial infarction

(2015) Dimitrios A. Stakos et al.   EUROPEAN HEART JOURNAL

A novel image-based quantitative method for the characterization of NETosis

(2015) Wenpu Zhao et al.   JOURNAL OF IMMUNOLOGICAL METHODS

How neutrophil extracellular traps orchestrate the local immune response in gout

(2015) Christian Maueröder et al.   JOURNAL OF MOLECULAR MEDICINE-JMM

Molecular mechanisms of NET formation and degradation revealed by intravital imaging in the liver vasculature

(2015) Elzbieta Kolaczkowska et al.   Nature Communications

Neutrophil extracellular traps regulate IL-1β-mediated inflammation in familial Mediterranean fever

(2014) Eirini Apostolidou et al.   ANNALS OF THE RHEUMATIC DISEASES

Acetylated histones contribute to the immunostimulatory potential of neutrophil extracellular traps in systemic lupus erythematosus

(2014) E. Pieterse et al.   CLINICAL AND EXPERIMENTAL IMMUNOLOGY

The necessity of and strategies for improving confidence in the accuracy of western blots

(2014) Rajeshwary Ghosh et al.   Expert Review of Proteomics

Release of Cystic Fibrosis Airway Inflammatory Markers from Pseudomonas aeruginosa-Stimulated Human Neutrophils Involves NADPH Oxidase-Dependent Extracellular DNA Trap Formation

(2014) D.-g. Yoo et al.   JOURNAL OF IMMUNOLOGY

Neutrophil extracellular traps promote differentiation and function of fibroblasts

(2014) Akrivi Chrysanthopoulou et al.   JOURNAL OF PATHOLOGY

Neutrophils at work

(2014) William M Nauseef et al.   NATURE IMMUNOLOGY

Aggregated neutrophil extracellular traps limit inflammation by degrading cytokines and chemokines

(2014) Christine Schauer et al.   NATURE MEDICINE

Tissue factor expression in neutrophil extracellular traps and neutrophil derived microparticles in antineutrophil cytoplasmic antibody associated vasculitis may promote thromboinflammation and the thrombophilic state associated with the disease

(2013) Konstantinos Kambas et al.   ANNALS OF THE RHEUMATIC DISEASES

Structural and functional alterations of cellular components as revealed by electron microscopy

(2013) Maria Condello et al.   MICROSCOPY RESEARCH AND TECHNIQUE

NETs Are a Source of Citrullinated Autoantigens and Stimulate Inflammatory Responses in Rheumatoid Arthritis

(2013) R. Khandpur et al.   Science Translational Medicine

Neutrophil extracellular traps: Is immunity the second function of chromatin?

(2012) Volker Brinkmann et al.   JOURNAL OF CELL BIOLOGY

Platelets induce neutrophil extracellular traps in transfusion-related acute lung injury

(2012) Axelle Caudrillier et al.   JOURNAL OF CLINICAL INVESTIGATION

Proteins derived from neutrophil extracellular traps may serve as self-antigens and mediate organ damage in autoimmune diseases

(2012) Jason S. Knight et al.   Frontiers in Immunology

Ultrastructural characterization of cystic fibrosis sputum using atomic force and scanning electron microscopy

(2011) Reinhard Manzenreiter et al.   Journal of Cystic Fibrosis

The Use of Spinning-Disk Confocal Microscopy for the Intravital Analysis of Platelet Dynamics in Response to Systemic and Local Inflammation

(2011) Craig N. Jenne et al.   PLoS One

Neutrophil Extracellular Trap Formation Is Associated with IL-1β and Autophagy-Related Signaling in Gout

(2011) Ioannis Mitroulis et al.   PLoS One

Multiphoton microscopy: An introduction to gastroenterologists

(2011) Hye Jin Cho   WORLD JOURNAL OF GASTROENTEROLOGY

Neutrophil extracellular trap cell death requires both autophagy and superoxide generation

(2010) Quinten Remijsen et al.   CELL RESEARCH

Neutrophil elastase and myeloperoxidase regulate the formation of neutrophil extracellular traps

(2010) Venizelos Papayannopoulos et al.   JOURNAL OF CELL BIOLOGY

Impairment of neutrophil extracellular trap degradation is associated with lupus nephritis

(2010) A. Hakkim et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Extracellular DNA traps promote thrombosis

(2010) T. A. Fuchs et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Impaired neutrophil extracellular trap (NET) formation: a novel innate immune deficiency of human neonates

(2009) C. C. Yost et al.   BLOOD

Viable neutrophils release mitochondrial DNA to form neutrophil extracellular traps

(2009) S Yousefi et al.   CELL DEATH AND DIFFERENTIATION

Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation

(2009) Yanming Wang et al.   JOURNAL OF CELL BIOLOGY

Mouse Neutrophil Extracellular Traps in Microbial Infections

(2009) David Ermert et al.   Journal of Innate Immunity

Netting neutrophils in autoimmune small-vessel vasculitis

(2009) Kai Kessenbrock et al.   NATURE MEDICINE

Visualization of neutrophil extracellular traps in TEM

(2007) Wolf Dietrich Krautgartner et al.   MICRON