Membrane-bound mRNA immunogens lower the threshold to activate HIV Env V2 apex-directed broadly neutralizing B cell precursors in humanized mice

Vaccination induces maturation in a mouse model of diverse unmutated VRC01-class precursors to HIV-neutralizing antibodies with >50% breadth

(2021) Xuejun Chen et al.   IMMUNITY

Safety and Efficacy of the BNT162b2 mRNA Covid-19 Vaccine through 6 Months

(2021) Stephen J. Thomas et al.   NEW ENGLAND JOURNAL OF MEDICINE

Vaccine genetics of IGHV1-2 VRC01-class broadly neutralizing antibody precursor naïve human B cells

(2021) Jeong Hyun Lee et al.   npj Vaccines

UCSF ChimeraX : Structure visualization for researchers, educators, and developers

(2020) Eric F. Pettersen et al.   PROTEIN SCIENCE

Multiplexed CRISPR/CAS9‐mediated engineering of pre‐clinical mouse models bearing native human B cell receptors

(2020) Xuesong Wang et al.   EMBO JOURNAL

SARS-CoV-2 mRNA Vaccines Foster Potent Antigen-Specific Germinal Center Responses Associated with Neutralizing Antibody Generation

(2020) Katlyn Lederer et al.   IMMUNITY

Efficacy and Safety of the mRNA-1273 SARS-CoV-2 Vaccine

(2020) Lindsey R. Baden et al.   NEW ENGLAND JOURNAL OF MEDICINE

High frequency of shared clonotypes in human B cell receptor repertoires

(2019) Cinque Soto et al.   NATURE

Slow Delivery Immunization Enhances HIV Neutralizing Antibody and Germinal Center Responses via Modulation of Immunodominance

(2019) Kimberly M. Cirelli et al.   CELL

Coevolution of HIV-1 and broadly neutralizing antibodies

(2019) Nicole A. Doria-Rose et al.   Current Opinion in HIV and AIDS

A generalized HIV vaccine design strategy for priming of broadly neutralizing antibody responses

(2019) Jon M. Steichen et al.   SCIENCE

Precursor Frequency and Affinity Determine B Cell Competitive Fitness in Germinal Centers, Tested with Germline-Targeting HIV Vaccine Immunogens

(2018) Robert K. Abbott et al.   IMMUNITY

mRNA vaccines — a new era in vaccinology

(2018) Norbert Pardi et al.   NATURE REVIEWS DRUG DISCOVERY

Anti–HIV-1 B cell responses are dependent on B cell precursor frequency and antigen-binding affinity

(2018) Pia Dosenovic et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The human naive B cell repertoire contains distinct subclasses for a germline-targeting HIV-1 vaccine immunogen

(2018) Colin Havenar-Daughton et al.   Science Translational Medicine

Co-evolution of HIV Envelope and Apex-Targeting Neutralizing Antibody Lineage Provides Benchmarks for Vaccine Design

(2018) Kimmo Rantalainen et al.   Cell Reports

Structural analysis of glycoproteins: building N-linked glycans with Coot

(2018) Paul Emsley et al.   Acta Crystallographica Section D-Structural Biology

One‐step CRISPR/Cas9 method for the rapid generation of human antibody heavy chain knock‐in mice

(2018) Ying‐Cing Lin et al.   EMBO JOURNAL

Recent progress in broadly neutralizing antibodies to HIV

(2018) Devin Sok et al.   NATURE IMMUNOLOGY

New tools for automated high-resolution cryo-EM structure determination in RELION-3

(2018) Jasenko Zivanov et al.   eLife

Glycans Function as Anchors for Antibodies and Help Drive HIV Broadly Neutralizing Antibody Development

(2017) Raiees Andrabi et al.   IMMUNITY

HIV Envelope Glycoform Heterogeneity and Localized Diversity Govern the Initiation and Maturation of a V2 Apex Broadly Neutralizing Antibody Lineage

(2017) Elise Landais et al.   IMMUNITY

Host controls of HIV broadly neutralizing antibody development

(2017) Garnett Kelsoe et al.   IMMUNOLOGICAL REVIEWS

Ontogeny-based immunogens for the induction of V2-directed HIV broadly neutralizing antibodies

(2017) Penny L. Moore et al.   IMMUNOLOGICAL REVIEWS

Germline-targeting immunogens

(2017) Leonidas Stamatatos et al.   IMMUNOLOGICAL REVIEWS

Native-like Env trimers as a platform for HIV-1 vaccine design

(2017) Rogier W. Sanders et al.   IMMUNOLOGICAL REVIEWS

Functional Stability of HIV-1 Envelope Trimer Affects Accessibility to Broadly Neutralizing Antibodies at Its Apex

(2017) Syna Kuriakose Gift et al.   JOURNAL OF VIROLOGY

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

Developing an HIV vaccine

(2017) Barton F. Haynes et al.   SCIENCE

Broadly Neutralizing Antibodies to HIV and Their Role in Vaccine Design

(2016) Dennis R. Burton et al.   Annual Review of Immunology

Tailored Immunogens Direct Affinity Maturation toward HIV Neutralizing Antibodies

(2016) Bryan Briney et al.   CELL

Sequential Immunization Elicits Broadly Neutralizing Anti-HIV-1 Antibodies in Ig Knockin Mice

(2016) Amelia Escolano et al.   CELL

Induction of HIV Neutralizing Antibody Lineages in Mice with Diverse Precursor Repertoires

(2016) Ming Tian et al.   CELL

HIV-Host Interactions: Implications for Vaccine Design

(2016) Barton F. Haynes et al.   Cell Host & Microbe

HIV Vaccine Design to Target Germline Precursors of Glycan-Dependent Broadly Neutralizing Antibodies

(2016) Jon M. Steichen et al.   IMMUNITY

Reverse vaccinology 2.0: Human immunology instructs vaccine antigen design

(2016) Rino Rappuoli et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Gctf: Real-time CTF determination and correction

(2016) Kai Zhang   JOURNAL OF STRUCTURAL BIOLOGY

Sequencing and cloning of antigen-specific antibodies from mouse memory B cells

(2016) Lotta von Boehmer et al.   Nature Protocols

Sustained antigen availability during germinal center initiation enhances antibody responses to vaccination

(2016) Hok Hei Tam et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Priming HIV-1 broadly neutralizing antibody precursors in human Ig loci transgenic mice

(2016) D. Sok et al.   SCIENCE

Broadly Neutralizing Antibody Responses in a Large Longitudinal Sub-Saharan HIV Primary Infection Cohort

(2016) Elise Landais et al.   PLoS Pathogens

Automated structure refinement of macromolecular assemblies from cryo-EM maps using Rosetta

(2016) Ray Yu-Ruei Wang et al.   eLife

Accelerated cryo-EM structure determination with parallelisation using GPUs in RELION-2

(2016) Dari Kimanius et al.   eLife

Immunization for HIV-1 Broadly Neutralizing Antibodies in Human Ig Knockin Mice

(2015) Pia Dosenovic et al.   CELL

Effect of vaccine administration modality on immunogenicity and efficacy

(2015) Lu Zhang et al.   Expert Review of Vaccines

Identification of Common Features in Prototype Broadly Neutralizing Antibodies to HIV Envelope V2 Apex to Facilitate Vaccine Design

(2015) Raiees Andrabi et al.   IMMUNITY

Expression kinetics of nucleoside-modified mRNA delivered in lipid nanoparticles to mice by various routes

(2015) Norbert Pardi et al.   JOURNAL OF CONTROLLED RELEASE

Murine Antibody Responses to Cleaved Soluble HIV-1 Envelope Trimers Are Highly Restricted in Specificity

(2015) Joyce K. Hu et al.   JOURNAL OF VIROLOGY

EMRinger: side chain–directed model and map validation for 3D cryo-electron microscopy

(2015) Benjamin A Barad et al.   NATURE METHODS

Privateer: software for the conformational validation of carbohydrate structures

(2015) Jon Agirre et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structures of HIV-1 Env V1V2 with broadly neutralizing antibodies reveal commonalities that enable vaccine design

(2015) Jason Gorman et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Priming a broadly neutralizing antibody response to HIV-1 using a germline-targeting immunogen

(2015) J. G. Jardine et al.   SCIENCE

Privateer: software for the conformational validation of carbohydrate structures

(2015) Jon Agirre et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structures of HIV-1 Env V1V2 with broadly neutralizing antibodies reveal commonalities that enable vaccine design

(2015) Jason Gorman et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Developmental pathway for potent V1V2-directed HIV-neutralizing antibodies

(2014) Nicole A. Doria-Rose et al.   NATURE

Vaccine Induction of Antibodies against a Structurally Heterogeneous Site of Immune Pressure within HIV-1 Envelope Protein Variable Regions 1 and 2

(2013) Hua-Xin Liao et al.   IMMUNITY

Engineering HIV envelope protein to activate germline B cell receptors of broadly neutralizing anti-CD4 binding site antibodies

(2013) Andrew T. McGuire et al.   JOURNAL OF EXPERIMENTAL MEDICINE

B Cells from Knock-in Mice Expressing Broadly Neutralizing HIV Antibody b12 Carry an Innocuous B Cell Receptor Responsive to HIV Vaccine Candidates

(2013) T. Ota et al.   JOURNAL OF IMMUNOLOGY

Structural basis for diverse N-glycan recognition by HIV-1–neutralizing V1–V2–directed antibody PG16

(2013) Marie Pancera et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Asymmetric recognition of the HIV-1 trimer by broadly neutralizing antibody PG9

(2013) Jean-Philippe Julien et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Delineating Antibody Recognition in Polyclonal Sera from Patterns of HIV-1 Isolate Neutralization

(2013) I. S. Georgiev et al.   SCIENCE

Antibodies in HIV-1 Vaccine Development and Therapy

(2013) F. Klein et al.   SCIENCE

Rational HIV Immunogen Design to Target Specific Germline B Cell Receptors

(2013) J. Jardine et al.   SCIENCE

Recombinant HIV Envelope Proteins Fail to Engage Germline Versions of Anti-CD4bs bNAbs

(2013) Sam Hoot et al.   PLoS Pathogens

The Effects of Somatic Hypermutation on Neutralization and Binding in the PGT121 Family of Broadly Neutralizing HIV Antibodies

(2013) Devin Sok et al.   PLoS Pathogens

Human Peripheral Blood Antibodies with Long HCDR3s Are Established Primarily at Original Recombination Using a Limited Subset of Germline Genes

(2012) Bryan S. Briney et al.   PLoS One

Complex-type N-glycan recognition by potent broadly neutralizing HIV antibodies

(2012) H. Mouquet et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of HIV-1 gp120 V1/V2 domain with broadly neutralizing antibody PG9

(2011) Jason S. McLellan et al.   NATURE

Quantitative analysis of cryo-EM density map segmentation by watershed and scale-space filtering, and fitting of structures by alignment to regions

(2010) Grigore D. Pintilie et al.   JOURNAL OF STRUCTURAL BIOLOGY

Crystal Structure of PG16 and Chimeric Dissection with Somatically Related PG9: Structure-Function Analysis of Two Quaternary-Specific Antibodies That Effectively Neutralize HIV-1

(2010) M. Pancera et al.   JOURNAL OF VIROLOGY

FastTree 2 – Approximately Maximum-Likelihood Trees for Large Alignments

(2010) Morgan N. Price et al.   PLoS One

Structure and function of broadly reactive antibody PG16 reveal an H3 subdomain that mediates potent neutralization of HIV-1

(2010) R. Pejchal et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural Basis for Broad and Potent Neutralization of HIV-1 by Antibody VRC01

(2010) T. Zhou et al.   SCIENCE

A Limited Number of Antibody Specificities Mediate Broad and Potent Serum Neutralization in Selected HIV-1 Infected Individuals

(2010) Laura M. Walker et al.   PLoS Pathogens

MolProbity: all-atom structure validation for macromolecular crystallography

(2009) Vincent B. Chen et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Germline-like predecessors of broadly neutralizing antibodies lack measurable binding to HIV-1 envelope glycoproteins: Implications for evasion of immune responses and design of vaccine immunogens

(2009) Xiaodong Xiao et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Broad and Potent Neutralizing Antibodies from an African Donor Reveal a New HIV-1 Vaccine Target

(2009) L. M. Walker et al.   SCIENCE

IMGT/V-QUEST: the highly customized and integrated system for IG and TR standardized V-J and V-D-J sequence analysis

(2008) X. Brochet et al.   NUCLEIC ACIDS RESEARCH